(Circular) (Six-base) MAPSORT of: pFN21ASDA1433.seq Check: 1116 from: 1 to: 6646 pFN21ASDA1433 6646 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4456 278 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3989 1937 Size: 2052 4594 Acc65I G'GTAC_C Cuts at: 3967 3967 Size: 6646 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 2424 4709 6093 6361 1632 Size: 602 190 2285 1384 268 1917 Fragments arranged by size: 2285 1917 1384 602 268 190 AfeI AGC'GCT Cuts at: 2537 2537 Size: 6646 AflII C'TTAA_G Cuts at: 820 1017 2270 820 Size: 197 1253 5196 Fragments arranged by size: 5196 1253 197 AflIII A'CryG_T Cuts at: 1203 5545 6522 1203 Size: 4342 977 1327 Fragments arranged by size: 4342 1327 977 AgeI A'CCGG_T Cuts at: 5477 5477 Size: 6646 AhdI GACnn_n'nnGTC Cuts at: 5375 5375 Size: 6646 AleI CACnn'nnGTG Cuts at: 1166 3542 1166 Size: 2376 4270 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2315 2403 1184 Size: 1131 88 5427 Fragments arranged by size: 5427 1131 88 AlwNI CAG_nnn'CTG Cuts at: 2927 3876 4389 5961 2927 Size: 949 513 1572 3612 Fragments arranged by size: 3612 1572 949 513 ApaLI G'TGCA_C Cuts at: 4703 5859 4703 Size: 1156 5490 ApoI r'AATT_y Cuts at: 1493 2465 3416 3955 4181 4333 6442 1493 Size: 972 951 539 226 152 2109 1697 Fragments arranged by size: 2109 1697 972 951 539 226 152 AseI AT'TA_AT Cuts at: 160 1032 5200 160 Size: 872 4168 1606 Fragments arranged by size: 4168 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 3971 1870 Size: 73 2028 4545 Fragments arranged by size: 4545 2028 73 BamHI G'GATC_C Cuts at: 3976 4343 3976 Size: 367 6279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2343 3967 5422 618 Size: 325 217 627 556 1624 1455 1842 Fragments arranged by size: 1842 1624 1455 627 556 325 217 BanII G_rGCy'C Cuts at: 1391 3257 3467 3704 3965 1391 Size: 1866 210 237 261 4072 Fragments arranged by size: 4072 1866 261 237 210 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3867 928 Size: 971 1968 3707 Fragments arranged by size: 3707 1968 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4837 4871 1903 Size: 34 2900 34 3678 Fragments arranged by size: 3678 2900 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2389 4538 5748 2389 Size: 2149 1210 3287 Fragments arranged by size: 3287 2149 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6646 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2120 5257 136 Size: 107 122 71 631 605 448 3137 1525 Fragments arranged by size: 3137 1525 631 605 448 122 107 71 BglII A'GATC_T Cuts at: 6641 6641 Size: 6646 BlpI GC'TnA_GC Cuts at: 2630 4063 2630 Size: 1433 5213 Bme1580I G_kGCm'C Cuts at: 1790 4707 5863 1790 Size: 2917 1156 2573 Fragments arranged by size: 2917 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6646 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3030 3225 4377 Size: 416 53 83 399 24 1613 195 1152 Cuts at: 4377 5335 442 Size: 958 1753 Fragments arranged by size: 1753 1613 1152 958 416 399 195 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3351 1056 Size: 2295 4351 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2469 2554 2469 Size: 85 6561 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3201 3535 3660 3696 5306 1239 Size: 1962 334 125 36 1610 2579 Fragments arranged by size: 2579 1962 1610 334 125 36 Bpu10I CC'TnA_GC Cuts at: 3698 3698 Size: 6646 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2387 2594 4771 5636 5934 2387 Size: 207 2177 865 298 3099 Fragments arranged by size: 3099 2177 865 298 207 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5309 882 Size: 617 3810 2219 Fragments arranged by size: 3810 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3943 4437 493 Size: 711 2739 494 2702 Fragments arranged by size: 2739 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6640 6640 Size: 6646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4453 4835 5470 275 Size: 53 83 186 936 2920 382 635 1451 Fragments arranged by size: 2920 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2796 5079 5477 5751 5898 6309 2796 Size: 2283 398 274 147 411 3133 Fragments arranged by size: 3133 2283 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2400 2430 3283 3313 3781 Size: 30 165 30 997 30 853 30 468 Cuts at: 3781 3811 1178 Size: 30 4013 Fragments arranged by size: 4013 997 853 468 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2546 3271 3274 1186 Size: 1360 725 3 4558 Fragments arranged by size: 4558 1360 725 3 BseYI C'CCAG_C Cuts at: 2319 3266 5849 6345 2319 Size: 947 2583 496 2620 Fragments arranged by size: 2620 2583 947 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2233 1163 Size: 573 497 5576 Fragments arranged by size: 5576 573 497 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4096 4857 5885 1540 Size: 275 186 2095 761 1028 2301 Fragments arranged by size: 2301 2095 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3257 3549 4707 4792 5863 3257 Size: 292 1158 85 1071 4040 Fragments arranged by size: 4040 1158 1071 292 85 BsmI GAATG_Cn' Cuts at: 4157 4250 4157 Size: 93 6553 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3257 3467 3549 3704 3965 4707 4792 Size: 399 1467 210 82 155 261 742 85 Cuts at: 4792 5863 1391 Size: 1071 2174 Fragments arranged by size: 2174 1467 1071 742 399 261 210 155 85 82 BspEI T'CCGG_A Cuts at: 6309 6309 Size: 6646 BspHI T'CATG_A Cuts at: 4535 6265 4535 Size: 1730 4916 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4001 844 Size: 3157 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4533 1394 Size: 429 2710 3507 Fragments arranged by size: 3507 2710 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5141 5315 65 Size: 1183 3893 174 1396 Fragments arranged by size: 3893 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3313 5290 5477 1700 Size: 110 1503 1977 187 2869 Fragments arranged by size: 2869 1977 1503 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 4706 5718 4706 Size: 1012 5634 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6646 BstBI TT'CG_AA Cuts at: 2007 5450 2007 Size: 3443 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6646 BstXI CCAn_nnnn'nTGG Cuts at: 2029 3520 2029 Size: 1491 5155 BstYI r'GATC_y Cuts at: 1972 3976 4343 4728 4745 6197 6283 6295 6641 Size: 2004 367 385 17 1452 86 12 346 Cuts at: 6641 1972 Size: 1977 Fragments arranged by size: 2004 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2989 3143 513 Size: 552 1924 154 4016 Fragments arranged by size: 4016 1924 552 154 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5907 Fragments arranged by size: 5907 683 56 BtsI GCAGTG_nn' Cuts at: 2294 3273 3636 3852 4162 4956 4976 2294 Size: 979 363 216 310 794 20 3964 Fragments arranged by size: 3964 979 794 363 310 216 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6611 DraI TTT'AAA Cuts at: 2832 3950 2832 Size: 1118 5528 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5653 809 Size: 507 4337 1802 Fragments arranged by size: 4337 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3070 4093 4982 Size: 54 1425 50 275 18 1240 1023 889 Cuts at: 4982 6611 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1240 1023 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4093 1537 Size: 275 2281 4090 Fragments arranged by size: 4090 2281 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2523 2648 2673 2784 3247 3313 3886 4576 Size: 1170 125 25 111 463 66 573 690 Cuts at: 4576 1353 Size: 3423 Fragments arranged by size: 3423 1170 690 573 463 125 111 66 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5233 5607 5753 387 Size: 1064 138 3644 374 146 1280 Fragments arranged by size: 3644 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3255 3255 Size: 6646 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2424 3201 3535 3660 3696 4709 Size: 393 602 190 777 334 125 36 1013 Cuts at: 4709 5306 6093 6361 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1013 787 777 602 597 393 334 268 190 125 36 EcoNI CCTnn'n_nnAGG Cuts at: 3675 3675 Size: 6646 EcoO109I rG'GnC_Cy Cuts at: 2098 2848 2098 Size: 750 5896 EcoRI G'AATT_C Cuts at: 3955 4333 3955 Size: 378 6268 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2102 2722 2102 Size: 620 6026 FspI TGC'GCA Cuts at: 5152 5152 Size: 6646 HaeII r_GCGC'y Cuts at: 1678 2539 5793 1678 Size: 861 3254 2531 Fragments arranged by size: 3254 2531 861 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2400 2432 3199 3231 3811 Size: 32 135 32 828 32 767 32 580 Cuts at: 3811 3843 5287 5319 5361 5393 1373 Size: 32 1444 32 42 32 2626 Fragments arranged by size: 2626 1444 828 767 580 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3990 4236 669 Size: 988 281 2052 246 3079 Fragments arranged by size: 3079 2052 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6646 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KpnI G_GTAC'C Cuts at: 3971 3971 Size: 6646 MfeI C'AATT_G Cuts at: 2888 4245 2888 Size: 1357 5289 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2442 5760 5944 1305 Size: 1137 3318 184 2007 Fragments arranged by size: 3318 2007 1137 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3072 10 Size: 54 1425 343 1240 3584 Fragments arranged by size: 3584 1425 1240 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 2567 2942 3542 4604 4963 5122 Size: 648 1257 144 375 600 1062 359 159 Cuts at: 5122 518 Size: 2042 Fragments arranged by size: 2042 1257 1062 648 600 375 359 159 144 MspA1I CmG'CkG Cuts at: 1606 2323 2383 2524 2690 3520 3876 4062 4739 Size: 717 60 141 166 830 356 186 677 Cuts at: 4739 5887 6132 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 830 717 677 356 245 186 166 141 60 NcoI C'CATG_G Cuts at: 513 1065 2989 513 Size: 552 1924 4170 Fragments arranged by size: 4170 1924 552 NdeI CA'TA_TG Cuts at: 387 3055 387 Size: 2668 3978 NheI G'CTAG_C Cuts at: 1052 3347 1052 Size: 2295 4351 NotI GC'GGCC_GC Cuts at: 4093 4093 Size: 6646 NspI r_CATG'y Cuts at: 1132 1229 2302 2314 2390 2428 4004 5549 1132 Size: 97 1073 12 76 38 1576 1545 2229 Fragments arranged by size: 2229 1576 1545 1073 97 76 38 12 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6646 PciI A'CATG_T Cuts at: 5545 5545 Size: 6646 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6646 PfoI T'CCnGG_A Cuts at: 3712 3712 Size: 6646 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4715 4747 6228 6260 4715 Size: 32 1481 32 5101 Fragments arranged by size: 5101 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 2848 2098 Size: 750 5896 PshAI GACnn'nnGTC Cuts at: 1613 3218 1613 Size: 1605 5041 PsiI TTA'TAA Cuts at: 4216 4216 Size: 6646 PstI C_TGCA'G Cuts at: 830 3115 3859 3998 830 Size: 2285 744 139 3478 Fragments arranged by size: 3478 2285 744 139 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6646 PvuII CAG'CTG Cuts at: 2323 2524 2690 3520 3876 2323 Size: 201 166 830 356 5093 Fragments arranged by size: 5093 830 356 201 166 SacI G_AGCT'C Cuts at: 3257 3257 Size: 6646 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SapI GCTCTTCn'nnn_ Cuts at: 2648 3313 3886 2648 Size: 665 573 5408 Fragments arranged by size: 5408 665 573 SbfI CC_TGCA'GG Cuts at: 3998 3998 Size: 6646 ScaI AGT'ACT Cuts at: 4894 6606 4894 Size: 1712 4934 SfcI C'TryA_G Cuts at: 826 1045 2192 3111 3458 3855 3994 5129 5810 Size: 219 1147 919 347 397 139 1135 681 Cuts at: 5810 6001 826 Size: 191 1471 Fragments arranged by size: 1471 1147 1135 919 681 397 347 219 191 139 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 3973 1872 Size: 2101 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2270 2366 2573 4750 5651 5913 Size: 197 926 327 96 207 2177 901 262 Cuts at: 5913 820 Size: 1553 Fragments arranged by size: 2177 1553 926 901 327 262 207 197 96 SnaBI TAC'GTA Cuts at: 493 3943 4437 493 Size: 3450 494 2702 Fragments arranged by size: 3450 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 2314 2390 4004 1132 Size: 1182 76 1614 3774 Fragments arranged by size: 3774 1614 1182 76 SspI AAT'ATT Cuts at: 5 52 4570 5 Size: 47 4518 2081 Fragments arranged by size: 4518 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6646 StyI C'CwwG_G Cuts at: 513 1065 2989 4085 513 Size: 552 1924 1096 3074 Fragments arranged by size: 3074 1924 1096 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3183 3778 3787 4673 4690 4843 5028 Size: 1255 1603 595 9 886 17 153 185 Cuts at: 5028 5473 325 Size: 445 1498 Fragments arranged by size: 1603 1498 1255 886 595 445 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4892 6604 96 Size: 274 80 33 51 1443 2915 1712 138 Fragments arranged by size: 2915 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3628 3660 3781 3813 6606 6638 3628 Size: 32 121 32 2793 32 3636 Fragments arranged by size: 3636 2793 121 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6646 XbaI T'CTAG_A Cuts at: 3982 3982 Size: 6646 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3449 3449 Size: 6646 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6646 XmaI C'CCGG_G Cuts at: 1870 3971 1870 Size: 2101 4545 XmnI GAAnn'nnTTC Cuts at: 4775 4775 Size: 6646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4454 276 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI