(Circular) (Six-base) MAPSORT of: pFN21ASDA1431.seq Check: 7886 from: 1 to: 7333 pFN21ASDA1431 7333 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5143 278 Size: 53 83 186 936 3607 2468 Fragments arranged by size: 3607 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4676 1937 Size: 2739 4594 Acc65I G'GTAC_C Cuts at: 4654 4654 Size: 7333 AclI AA'CG_TT Cuts at: 1563 5460 5833 1563 Size: 3897 373 3063 Fragments arranged by size: 3897 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2997 3547 3631 3715 3883 4051 4471 5396 Size: 1365 550 84 84 168 168 420 925 Cuts at: 5396 6780 7048 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1365 925 550 420 268 168 168 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7136 AflIII A'CryG_T Cuts at: 1203 6232 7209 1203 Size: 5029 977 1327 Fragments arranged by size: 5029 1327 977 AgeI A'CCGG_T Cuts at: 6164 6164 Size: 7333 AhdI GACnn_n'nnGTC Cuts at: 6062 6062 Size: 7333 AleI CACnn'nnGTG Cuts at: 1166 4110 1166 Size: 2944 4389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7333 AlwNI CAG_nnn'CTG Cuts at: 5076 6648 5076 Size: 1572 5761 ApaLI G'TGCA_C Cuts at: 5390 6546 5390 Size: 1156 6177 ApoI r'AATT_y Cuts at: 1493 2773 3220 3342 3597 4521 4592 4642 4868 Size: 1280 447 122 255 924 71 50 226 Cuts at: 4868 5020 7129 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1280 924 447 255 226 152 122 71 50 AseI AT'TA_AT Cuts at: 160 1032 5887 160 Size: 872 4855 1606 Fragments arranged by size: 4855 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7333 AvaI C'yCGr_G Cuts at: 1870 1943 2068 2097 2151 4658 1870 Size: 73 125 29 54 2507 4545 Fragments arranged by size: 4545 2507 125 73 54 29 BamHI G'GATC_C Cuts at: 4663 5030 4663 Size: 367 6966 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2538 4654 6109 618 Size: 325 217 627 291 460 2116 1455 1842 Fragments arranged by size: 2116 1842 1455 627 460 325 291 217 BanII G_rGCy'C Cuts at: 1391 2233 2880 3320 4652 1391 Size: 842 647 440 1332 4072 Fragments arranged by size: 4072 1332 842 647 440 BbeI G_GCGC'C Cuts at: 2082 2082 Size: 7333 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4477 928 Size: 971 2578 3784 Fragments arranged by size: 3784 2578 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5524 5558 1903 Size: 34 3587 34 3678 Fragments arranged by size: 3678 3587 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5225 6435 5225 Size: 1210 6123 BclI T'GATC_A Cuts at: 1548 1548 Size: 7333 BfrBI ATG'CAT Cuts at: 3743 3743 Size: 7333 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2138 5944 136 Size: 107 122 71 631 605 466 3806 1525 Fragments arranged by size: 3806 1525 631 605 466 122 107 71 BglII A'GATC_T Cuts at: 4454 7328 4454 Size: 2874 4459 BlpI GC'TnA_GC Cuts at: 2838 4750 2838 Size: 1912 5421 Bme1580I G_kGCm'C Cuts at: 1790 2226 2543 5394 6550 1790 Size: 436 317 2851 1156 2573 Fragments arranged by size: 2851 2573 1156 436 317 BmgBI CAC'GTC Cuts at: 1318 4566 1318 Size: 3248 4085 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2686 3531 3867 Size: 416 53 83 399 24 1269 845 336 Cuts at: 3867 5064 6022 442 Size: 1197 958 1753 Fragments arranged by size: 1753 1269 1197 958 845 416 399 336 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7333 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3339 3339 Size: 7333 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2112 2300 2672 3372 3526 3627 3795 3963 Size: 873 188 372 700 154 101 168 168 Cuts at: 3963 4299 4467 4551 5993 1239 Size: 336 168 84 1442 2579 Fragments arranged by size: 2579 1442 873 700 372 336 188 168 168 168 154 101 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2152 2567 5458 6323 6621 2152 Size: 415 2891 865 298 2864 Fragments arranged by size: 2891 2864 865 415 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2933 5996 882 Size: 617 1434 3063 2219 Fragments arranged by size: 3063 2219 1434 617 BsaAI yAC'GTr Cuts at: 493 1204 4630 5124 493 Size: 711 3426 494 2702 Fragments arranged by size: 3426 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7327 7327 Size: 7333 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 2079 5140 5522 Size: 53 83 186 936 527 19 3061 382 Cuts at: 5522 6157 275 Size: 635 1451 Fragments arranged by size: 3061 1451 936 635 527 382 186 83 53 19 BsaWI w'CCGG_w Cuts at: 3689 5766 6164 6438 6585 6996 3689 Size: 2077 398 274 147 411 4026 Fragments arranged by size: 4026 2077 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2360 2390 1178 Size: 30 165 30 957 30 6121 Fragments arranged by size: 6121 957 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2214 2381 2470 1186 Size: 1028 167 89 6049 Fragments arranged by size: 6049 1028 167 89 BseYI C'CCAG_C Cuts at: 2446 2761 6536 7032 2446 Size: 315 3775 496 2747 Fragments arranged by size: 3775 2747 496 315 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4172 1163 Size: 573 2436 4324 Fragments arranged by size: 4324 2436 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4783 5544 6572 1540 Size: 275 186 2782 761 1028 2301 Fragments arranged by size: 2782 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2233 3320 5394 5479 6550 2233 Size: 1087 2074 85 1071 3016 Fragments arranged by size: 3016 2074 1087 1071 85 BsmI GAATG_Cn' Cuts at: 4058 4232 4301 4844 4937 4058 Size: 174 69 543 93 6454 Fragments arranged by size: 6454 543 174 93 69 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2233 2543 2880 3320 4652 5394 Size: 399 436 7 310 337 440 1332 742 Cuts at: 5394 5479 6550 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1332 1071 742 440 436 399 337 310 85 7 BspEI T'CCGG_A Cuts at: 6996 6996 Size: 7333 BspHI T'CATG_A Cuts at: 5222 6952 5222 Size: 1730 5603 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4688 844 Size: 3844 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2065 2204 5220 1394 Size: 429 242 139 3016 3507 Fragments arranged by size: 3507 3016 429 242 139 BsrDI GCAATG_nn' Cuts at: 65 1248 5828 6002 65 Size: 1183 4580 174 1396 Fragments arranged by size: 4580 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5977 6164 1700 Size: 110 4167 187 2869 Fragments arranged by size: 4167 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2403 2932 3670 4342 96 Size: 2307 529 738 672 3087 Fragments arranged by size: 3087 2307 738 672 529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7333 BssSI C'ACGA_G Cuts at: 5393 6405 5393 Size: 1012 6321 BstAPI GCAn_nnn'nTGC Cuts at: 794 4053 794 Size: 3259 4074 BstBI TT'CG_AA Cuts at: 2007 6137 2007 Size: 4130 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7333 BstYI r'GATC_y Cuts at: 1972 4454 4579 4663 5030 5415 5432 6884 6970 Size: 2482 125 84 367 385 17 1452 86 Cuts at: 6970 6982 7328 1972 Size: 12 346 1977 Fragments arranged by size: 2482 1977 1452 385 367 346 125 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6781 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2427 4247 487 Size: 683 56 1201 1820 3573 Fragments arranged by size: 3573 1820 1201 683 56 BtsI GCAGTG_nn' Cuts at: 2247 2625 4306 4849 5643 5663 2247 Size: 378 1681 543 794 20 3917 Fragments arranged by size: 3917 1681 794 543 378 20 ClaI AT'CG_AT Cuts at: 1554 3546 1554 Size: 1992 5341 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2351 2386 4557 4592 529 Size: 35 1787 35 2171 35 3270 Fragments arranged by size: 3270 2171 1787 35 35 35 DraI TTT'AAA Cuts at: 4637 4637 Size: 7333 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6340 809 Size: 507 5024 1802 Fragments arranged by size: 5024 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4195 4780 5669 Size: 54 1425 50 275 18 2365 585 889 Cuts at: 5669 7298 8 Size: 1629 43 Fragments arranged by size: 2365 1629 1425 889 585 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4780 1537 Size: 275 2968 4090 Fragments arranged by size: 4090 2968 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 3183 5263 1353 Size: 906 924 2080 3423 Fragments arranged by size: 3423 2080 924 906 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2735 5920 6294 6440 387 Size: 1064 138 1146 3185 374 146 1280 Fragments arranged by size: 3185 1280 1146 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2231 3318 2231 Size: 1087 6246 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2112 2300 2672 2997 3372 3526 3547 Size: 393 480 188 372 325 375 154 21 Cuts at: 3547 3627 3631 3715 3795 3883 3963 4051 4299 Size: 80 4 84 80 88 80 88 248 Cuts at: 4299 4467 4471 4551 5396 5993 6780 7048 1239 Size: 168 4 80 845 597 787 268 1524 Fragments arranged by size: 1524 845 787 597 480 393 375 372 325 268 248 188 168 154 88 88 84 80 80 80 80 21 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 3128 3128 Size: 7333 EcoO109I rG'GnC_Cy Cuts at: 2216 2216 Size: 7333 EcoRI G'AATT_C Cuts at: 3342 3597 4521 4642 5020 3342 Size: 255 924 121 378 5655 Fragments arranged by size: 5655 924 378 255 121 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2608 2608 Size: 7333 FspI TGC'GCA Cuts at: 5839 5839 Size: 7333 HaeII r_GCGC'y Cuts at: 1678 2082 6480 1678 Size: 404 4398 2531 Fragments arranged by size: 4398 2531 404 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 2801 2833 2837 Size: 32 135 32 890 32 307 32 4 Cuts at: 2837 2869 2981 3013 3389 3421 3499 3531 4148 Size: 32 112 32 376 32 78 32 617 Cuts at: 4148 4180 5974 6006 6048 6080 1373 Size: 32 1794 32 42 32 2626 Fragments arranged by size: 2626 1794 890 617 376 307 135 112 78 42 32 32 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 2740 4542 4677 4923 669 Size: 988 281 802 1802 135 246 3079 Fragments arranged by size: 3079 1802 988 802 281 246 135 HindIII A'AGCT_T Cuts at: 748 3065 3275 3471 3810 748 Size: 2317 210 196 339 4271 Fragments arranged by size: 4271 2317 339 210 196 HpaI GTT'AAC Cuts at: 4923 4923 Size: 7333 KasI G'GCGC_C Cuts at: 2078 2078 Size: 7333 KpnI G_GTAC'C Cuts at: 4658 4658 Size: 7333 MfeI C'AATT_G Cuts at: 4932 4932 Size: 7333 MluI A'CGCG_T Cuts at: 7209 7209 Size: 7333 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2400 2466 6447 6631 1305 Size: 1095 66 3981 184 2007 Fragments arranged by size: 3981 2007 1095 184 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 4197 10 Size: 54 1425 343 2365 3146 Fragments arranged by size: 3146 2365 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3204 3706 4042 4110 4378 5291 5650 Size: 648 2038 502 336 68 268 913 359 Cuts at: 5650 5809 518 Size: 159 2042 Fragments arranged by size: 2042 2038 913 648 502 359 336 268 159 68 MspA1I CmG'CkG Cuts at: 1606 4749 5426 6574 6819 1606 Size: 3143 677 1148 245 2120 Fragments arranged by size: 3143 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2079 2079 Size: 7333 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6781 NdeI CA'TA_TG Cuts at: 387 2971 3703 4039 4375 387 Size: 2584 732 336 336 3345 Fragments arranged by size: 3345 2584 732 336 336 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7333 NotI GC'GGCC_GC Cuts at: 4780 4780 Size: 7333 NsiI A_TGCA'T Cuts at: 3745 3745 Size: 7333 NspI r_CATG'y Cuts at: 1132 1229 4548 4691 6236 1132 Size: 97 3319 143 1545 2229 Fragments arranged by size: 3319 2229 1545 143 97 PasI CC'CwG_GG Cuts at: 1348 2320 1348 Size: 972 6361 PciI A'CATG_T Cuts at: 6232 6232 Size: 7333 PflMI CCAn_nnn'nTGG Cuts at: 1108 2326 1108 Size: 1218 6115 PmeI GTTT'AAAC Cuts at: 4637 4637 Size: 7333 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5402 5434 6915 6947 5402 Size: 32 1481 32 5788 Fragments arranged by size: 5788 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7333 PsiI TTA'TAA Cuts at: 3366 4461 4903 3366 Size: 1095 442 5796 Fragments arranged by size: 5796 1095 442 PstI C_TGCA'G Cuts at: 830 4685 830 Size: 3855 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7333 SacI G_AGCT'C Cuts at: 2233 3320 2233 Size: 1087 6246 SalI G'TCGA_C Cuts at: 1936 4675 1936 Size: 2739 4594 SapI GCTCTTCn'nnn_ Cuts at: 2259 2259 Size: 7333 SbfI CC_TGCA'GG Cuts at: 4685 4685 Size: 7333 ScaI AGT'ACT Cuts at: 2657 5581 7293 2657 Size: 2924 1712 2697 Fragments arranged by size: 2924 2697 1712 SfcI C'TryA_G Cuts at: 826 1045 2118 2349 2428 4681 5816 6497 6688 Size: 219 1073 231 79 2253 1135 681 191 Cuts at: 6688 826 Size: 1471 Fragments arranged by size: 2253 1471 1135 1073 681 231 219 191 79 SfoI GGC'GCC Cuts at: 2080 2080 Size: 7333 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7333 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7333 SmaI CCC'GGG Cuts at: 1872 2070 2099 2153 4660 1872 Size: 198 29 54 2507 4545 Fragments arranged by size: 4545 2507 198 54 29 SmlI C'TyrA_G Cuts at: 820 1017 1943 2167 2582 5437 6338 6600 820 Size: 197 926 224 415 2855 901 262 1553 Fragments arranged by size: 2855 1553 926 901 415 262 224 197 SnaBI TAC'GTA Cuts at: 493 4630 5124 493 Size: 4137 494 2702 Fragments arranged by size: 4137 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7333 SphI G_CATG'C Cuts at: 1132 4691 1132 Size: 3559 3774 SspI AAT'ATT Cuts at: 5 52 5257 5 Size: 47 5205 2081 Fragments arranged by size: 5205 2081 47 StuI AGG'CCT Cuts at: 1517 3389 4148 1517 Size: 1872 759 4702 Fragments arranged by size: 4702 1872 759 StyI C'CwwG_G Cuts at: 513 1065 2136 2504 4772 513 Size: 552 1071 368 2268 3074 Fragments arranged by size: 3074 2268 1071 552 368 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2775 4001 5360 5377 5530 5715 6160 Size: 1255 1195 1226 1359 17 153 185 445 Cuts at: 6160 325 Size: 1498 Fragments arranged by size: 1498 1359 1255 1226 1195 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2403 2655 2932 Size: 274 80 33 51 1443 426 252 277 Cuts at: 2932 3670 3766 4169 4342 5579 7291 96 Size: 738 96 403 173 1237 1712 138 Fragments arranged by size: 1712 1443 1237 738 426 403 277 274 252 173 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2239 2271 2360 2392 2817 2849 4555 4587 7293 Size: 32 89 32 425 32 1706 32 2706 Cuts at: 7293 7325 2239 Size: 32 2247 Fragments arranged by size: 2706 2247 1706 425 89 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7333 XbaI T'CTAG_A Cuts at: 4669 4669 Size: 7333 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7333 XmaI C'CCGG_G Cuts at: 1870 2068 2097 2151 4658 1870 Size: 198 29 54 2507 4545 Fragments arranged by size: 4545 2507 198 54 29 XmnI GAAnn'nnTTC Cuts at: 2591 2774 2952 3473 3560 4357 4400 5462 2591 Size: 183 178 521 87 797 43 1062 4462 Fragments arranged by size: 4462 1062 797 521 183 178 87 43 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5141 276 Size: 53 83 186 936 3607 2468 Fragments arranged by size: 3607 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI