Restriction map for
pFN21ASDA1391
(Circular) (Six-base) MAPSORT of: pFN21ASDA1391.seq Check: 6344 from: 1 to: 8302
pFN21ASDA1391 8302 bp
With 182 enzymes: SgfI *
January 16, 2009 15:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6112 278
Size: 53 83 186 936 4576 2468
Fragments arranged by size:
4576 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5645 1937
Size: 3708 4594
Acc65I G'GTAC_C
Cuts at: 5623 5623
Size: 8302
AclI AA'CG_TT
Cuts at: 1563 6429 6802 1563
Size: 4866 373 3063
Fragments arranged by size:
4866 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2546 2969 3199 3682 4271 5337 6365 7749
Size: 914 423 230 483 589 1066 1028 1384
Cuts at: 7749 8017 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1066 1028 914 589 483 423
268 230
AfeI AGC'GCT
Cuts at: 2161 2161
Size: 8302
AflII C'TTAA_G
Cuts at: 820 1017 5305 5420 820
Size: 197 4288 115 3702
Fragments arranged by size:
4288 3702 197 115
AflIII A'CryG_T
Cuts at: 1203 2703 4286 7201 8178 1203
Size: 1500 1583 2915 977 1327
Fragments arranged by size:
2915 1583 1500 1327 977
AgeI A'CCGG_T
Cuts at: 7133 7133
Size: 8302
AhdI GACnn_n'nnGTC
Cuts at: 7031 7031
Size: 8302
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8302
AlwNI CAG_nnn'CTG
Cuts at: 2980 4316 6045 7617 2980
Size: 1336 1729 1572 3665
Fragments arranged by size:
3665 1729 1572 1336
ApaI G_GGCC'C
Cuts at: 4051 4051
Size: 8302
ApaLI G'TGCA_C
Cuts at: 4063 6359 7515 4063
Size: 2296 1156 4850
Fragments arranged by size:
4850 2296 1156
ApoI r'AATT_y
Cuts at: 1493 2674 3114 3265 3614 4608 5611 5837 5989
Size: 1181 440 151 349 994 1003 226 152
Cuts at: 5989 8098 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1181 1003 994 440 349 226
152 151
AseI AT'TA_AT
Cuts at: 160 1032 4719 6856 160
Size: 872 3687 2137 1606
Fragments arranged by size:
3687 2137 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8302
AvaI C'yCGr_G
Cuts at: 1870 1943 2922 3905 5627 1870
Size: 73 979 983 1722 4545
Fragments arranged by size:
4545 1722 983 979 73
BamHI G'GATC_C
Cuts at: 5632 5999 5632
Size: 367 7935
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5623 7078 618
Size: 325 217 627 3836 1455 1842
Fragments arranged by size:
3836 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2901 4051 4140 4566 5471 5621 1391
Size: 1510 1150 89 426 905 150 4072
Fragments arranged by size:
4072 1510 1150 905 426 150 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4248 4581 4986 928
Size: 971 2349 333 405 4244
Fragments arranged by size:
4244 2349 971 405 333
BbvCI CC'TCA_GC
Cuts at: 5500 5500
Size: 8302
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6493 6527 1903
Size: 34 4556 34 3678
Fragments arranged by size:
4556 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4493 6194 7404 4493
Size: 1701 1210 5391
Fragments arranged by size:
5391 1701 1210
BclI T'GATC_A
Cuts at: 1548 3376 1548
Size: 1828 6474
BfrBI ATG'CAT
Cuts at: 5104 5104
Size: 8302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6913 136
Size: 107 122 71 631 605 5241 1525
Fragments arranged by size:
5241 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3017 3367 8297 3017
Size: 350 4930 3022
Fragments arranged by size:
4930 3022 350
BlpI GC'TnA_GC
Cuts at: 5719 5719
Size: 8302
Bme1580I G_kGCm'C
Cuts at: 1790 4051 4067 6363 7519 1790
Size: 2261 16 2296 1156 2573
Fragments arranged by size:
2573 2296 2261 1156 16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8302
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3040 3391 6033
Size: 416 53 83 399 24 1623 351 2642
Cuts at: 6033 6991 442
Size: 958 1753
Fragments arranged by size:
2642 1753 1623 958 416 399 351 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8302
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2178 3754 4954 2178
Size: 1576 1200 5526
Fragments arranged by size:
5526 1576 1200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2514 2876 3291 3577 4253 6962 1239
Size: 1061 214 362 415 286 676 2709 2579
Fragments arranged by size:
2709 2579 1061 676 415 362 286 214
Bpu10I CC'TnA_GC
Cuts at: 3929 5500 3929
Size: 1571 6731
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4514 6427 7292 7590 4514
Size: 1913 865 298 5226
Fragments arranged by size:
5226 1913 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3391 4655 6965 882
Size: 617 1892 1264 2310 2219
Fragments arranged by size:
2310 2219 1892 1264 617
BsaAI yAC'GTr
Cuts at: 493 1204 4390 5599 6093 493
Size: 711 3186 1209 494 2702
Fragments arranged by size:
3186 2702 1209 711 494
BsaBI GATnn'nnATC
Cuts at: 8296 8296
Size: 8302
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6109 6491 7126 275
Size: 53 83 186 936 4576 382 635 1451
Fragments arranged by size:
4576 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5018 6735 7133 7407 7554 7965 5018
Size: 1717 398 274 147 411 5355
Fragments arranged by size:
5355 1717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3264 3294 4924 4954 1178
Size: 30 165 30 1861 30 1630 30 4526
Fragments arranged by size:
4526 1861 1630 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2065 2167 2170 3760 4679 1186
Size: 879 102 3 1590 919 4809
Fragments arranged by size:
4809 1590 919 879 102 3
BseYI C'CCAG_C
Cuts at: 2040 2954 2973 4070 4139 5059 5123 7505 8001
Size: 914 19 1097 69 920 64 2382 496
Cuts at: 8001 2040
Size: 2341
Fragments arranged by size:
2382 2341 1097 920 914 496 69 64
19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2101 2829 5354 1163
Size: 573 365 728 2525 4111
Fragments arranged by size:
4111 2525 728 573 365
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5752 6513 7541 1540
Size: 275 186 3751 761 1028 2301
Fragments arranged by size:
3751 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2267 4067 5471 6363 6448 7519 2267
Size: 1800 1404 892 85 1071 3050
Fragments arranged by size:
3050 1800 1404 1071 892 85
BsmI GAATG_Cn'
Cuts at: 2607 3739 5028 5092 5104 5813 5906 2607
Size: 1132 1289 64 12 709 93 5003
Fragments arranged by size:
5003 1289 1132 709 93 64 12
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2267 2901 4051 4067 4140 4566 5471
Size: 399 477 634 1150 16 73 426 905
Cuts at: 5471 5621 6363 6448 7519 1391
Size: 150 742 85 1071 2174
Fragments arranged by size:
2174 1150 1071 905 742 634 477 426
399 150 85 73 16
BspEI T'CCGG_A
Cuts at: 5018 7965 5018
Size: 2947 5355
BspHI T'CATG_A
Cuts at: 6191 7921 6191
Size: 1730 6572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5657 844
Size: 4813 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2329 6189 1394
Size: 429 506 3860 3507
Fragments arranged by size:
3860 3507 506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5008 6797 6971 65
Size: 1183 3760 1789 174 1396
Fragments arranged by size:
3760 1789 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3997 4122 6946 7133 1700
Size: 110 2187 125 2824 187 2869
Fragments arranged by size:
2869 2824 2187 187 125 110
BsrGI T'GTAC_A
Cuts at: 96 4014 96
Size: 3918 4384
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8302
BssSI C'ACGA_G
Cuts at: 6362 7374 6362
Size: 1012 7290
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4097 4100 794
Size: 3303 3 4996
Fragments arranged by size:
4996 3303 3
BstBI TT'CG_AA
Cuts at: 2007 7106 2007
Size: 5099 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8302
BstXI CCAn_nnnn'nTGG
Cuts at: 2962 4429 2962
Size: 1467 6835
BstYI r'GATC_y
Cuts at: 1972 3017 3367 4023 5632 5999 6384 6401 7853
Size: 1045 350 656 1609 367 385 17 1452
Cuts at: 7853 7939 7951 8297 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1609 1452 1045 656 385 367 350
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3476 3665 4112 4175 3476
Size: 189 447 63 7603
Fragments arranged by size:
7603 447 189 63
BtgI C'CryG_G
Cuts at: 513 1065 2022 5082 513
Size: 552 957 3060 3733
Fragments arranged by size:
3733 3060 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2045 3063 487
Size: 683 56 819 1018 5726
Fragments arranged by size:
5726 1018 819 683 56
BtsI GCAGTG_nn'
Cuts at: 2939 3829 4028 5276 5818 6612 6632 2939
Size: 890 199 1248 542 794 20 4609
Fragments arranged by size:
4609 1248 890 794 542 199 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2421 2456 529
Size: 35 1857 35 6375
Fragments arranged by size:
6375 1857 35 35
DraI TTT'AAA
Cuts at: 3007 4294 5606 3007
Size: 1287 1312 5703
Fragments arranged by size:
5703 1312 1287
DraIII CAC_nnn'GTG
Cuts at: 4915 4915
Size: 8302
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2092 7309 809
Size: 507 776 5217 1802
Fragments arranged by size:
5217 1802 776 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2290 2364 2514
Size: 54 1425 50 275 18 460 74 150
Cuts at: 2514 3844 4419 5148 5749 6638 8267 8
Size: 1330 575 729 601 889 1629 43
Fragments arranged by size:
1629 1425 1330 889 729 601 575 460
275 150 74 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5749 1537
Size: 275 3937 4090
Fragments arranged by size:
4090 3937 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2083 2597 2769 3408 3701 5121 5199 5476
Size: 730 514 172 639 293 1420 78 277
Cuts at: 5476 6232 1353
Size: 756 3423
Fragments arranged by size:
3423 1420 756 730 639 514 293 277
172 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6889 7263 7409 387
Size: 1064 138 5300 374 146 1280
Fragments arranged by size:
5300 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5469 5469
Size: 8302
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2514 2546 2876 2969 3199 3291
Size: 393 668 214 32 330 93 230 92
Cuts at: 3291 3577 3682 4253 4271 5337 6365 6962 7749
Size: 286 105 571 18 1066 1028 597 787
Cuts at: 7749 8017 1239
Size: 268 1524
Fragments arranged by size:
1524 1066 1028 787 668 597 571 393
330 286 268 230 214 105 93 92
32 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3096 4174 4271 4600 3096
Size: 1078 97 329 6798
Fragments arranged by size:
6798 1078 329 97
EcoO109I rG'GnC_Cy
Cuts at: 3194 4047 4048 5228 5546 3194
Size: 853 1 1180 318 5950
Fragments arranged by size:
5950 1180 853 318 1
EcoRI G'AATT_C
Cuts at: 2674 3265 5611 5989 2674
Size: 591 2346 378 4987
Fragments arranged by size:
4987 2346 591 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4381 4381
Size: 8302
FspI TGC'GCA
Cuts at: 6808 6808
Size: 8302
HaeII r_GCGC'y
Cuts at: 1678 2074 2163 4555 7449 1678
Size: 396 89 2392 2894 2531
Fragments arranged by size:
2894 2531 2392 396 89
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3281 3313 3669 3701 4924
Size: 32 135 32 1709 32 356 32 1223
Cuts at: 4924 4956 6943 6975 7017 7049 1373
Size: 32 1987 32 42 32 2626
Fragments arranged by size:
2626 1987 1709 1223 356 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2233 5646 5892 669
Size: 988 281 295 3413 246 3079
Fragments arranged by size:
3413 3079 988 295 281 246
HindIII A'AGCT_T
Cuts at: 748 4163 4363 748
Size: 3415 200 4687
Fragments arranged by size:
4687 3415 200
HpaI GTT'AAC
Cuts at: 5892 5892
Size: 8302
KpnI G_GTAC'C
Cuts at: 5627 5627
Size: 8302
MfeI C'AATT_G
Cuts at: 5901 5901
Size: 8302
MluI A'CGCG_T
Cuts at: 8178 8178
Size: 8302
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7416 7600 1305
Size: 6111 184 2007
Fragments arranged by size:
6111 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 2516 3846 4421 5150
Size: 54 1425 343 534 150 1330 575 729
Cuts at: 5150 10
Size: 3162
Fragments arranged by size:
3162 1425 1330 729 575 534 343 150
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2260 4010 4427 6260 6619 6778 518
Size: 648 1094 1750 417 1833 359 159 2042
Fragments arranged by size:
2042 1833 1750 1094 648 417 359 159
MspA1I CmG'CkG
Cuts at: 1606 2115 4088 4212 4638 5315 5718 6395 7543
Size: 509 1973 124 426 677 403 677 1148
Cuts at: 7543 7788 1606
Size: 245 2120
Fragments arranged by size:
2120 1973 1148 677 677 509 426 403
245 124
NcoI C'CATG_G
Cuts at: 513 1065 2022 5082 513
Size: 552 957 3060 3733
Fragments arranged by size:
3733 3060 957 552
NdeI CA'TA_TG
Cuts at: 387 2812 387
Size: 2425 5877
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8302
NotI GC'GGCC_GC
Cuts at: 5749 5749
Size: 8302
NsiI A_TGCA'T
Cuts at: 5106 5106
Size: 8302
NspI r_CATG'y
Cuts at: 1132 1229 2241 2707 4290 5252 5660 7205 1132
Size: 97 1012 466 1583 962 408 1545 2229
Fragments arranged by size:
2229 1583 1545 1012 962 466 408 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8302
PciI A'CATG_T
Cuts at: 2703 4286 7201 2703
Size: 1583 2915 3804
Fragments arranged by size:
3804 2915 1583
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2795 5088 5130 5511 1108
Size: 1687 2293 42 381 3899
Fragments arranged by size:
3899 2293 1687 381 42
PfoI T'CCnGG_A
Cuts at: 2175 2892 3346 4269 5367 2175
Size: 717 454 923 1098 5110
Fragments arranged by size:
5110 1098 923 717 454
PmeI GTTT'AAAC
Cuts at: 5606 5606
Size: 8302
PmlI CAC'GTG
Cuts at: 4390 4390
Size: 8302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6371 6403 7884 7916 6371
Size: 32 1481 32 6757
Fragments arranged by size:
6757 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3194 5228 3194
Size: 2034 6268
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8302
PsiI TTA'TAA
Cuts at: 5872 5872
Size: 8302
PspOMI G'GGCC_C
Cuts at: 4047 4047
Size: 8302
PstI C_TGCA'G
Cuts at: 830 2971 3245 4093 5654 830
Size: 2141 274 848 1561 3478
Fragments arranged by size:
3478 2141 1561 848 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8302
PvuII CAG'CTG
Cuts at: 2115 4088 4212 4638 5315 2115
Size: 1973 124 426 677 5102
Fragments arranged by size:
5102 1973 677 426 124
SacI G_AGCT'C
Cuts at: 5471 5471
Size: 8302
SalI G'TCGA_C
Cuts at: 1936 5644 1936
Size: 3708 4594
SapI GCTCTTCn'nnn_
Cuts at: 2597 5476 2597
Size: 2879 5423
SbfI CC_TGCA'GG
Cuts at: 5654 5654
Size: 8302
ScaI AGT'ACT
Cuts at: 6550 8262 6550
Size: 1712 6590
SfcI C'TryA_G
Cuts at: 826 1045 2967 3241 4089 5386 5550 5650 6785
Size: 219 1922 274 848 1297 164 100 1135
Cuts at: 6785 7466 7657 826
Size: 681 191 1471
Fragments arranged by size:
1922 1471 1297 1135 848 681 274 219
191 164 100
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8302
SgrAI Cr'CCGG_yG
Cuts at: 1700 4122 1700
Size: 2422 5880
SmaI CCC'GGG
Cuts at: 1872 5629 1872
Size: 3757 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4529 5305 5420 6406 7307 7569
Size: 197 926 2586 776 115 986 901 262
Cuts at: 7569 820
Size: 1553
Fragments arranged by size:
2586 1553 986 926 901 776 262 197
115
SnaBI TAC'GTA
Cuts at: 493 5599 6093 493
Size: 5106 494 2702
Fragments arranged by size:
5106 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8302
SphI G_CATG'C
Cuts at: 1132 5660 1132
Size: 4528 3774
SspI AAT'ATT
Cuts at: 5 52 3130 3343 6226 5
Size: 47 3078 213 2883 2081
Fragments arranged by size:
3078 2883 2081 213 47
StuI AGG'CCT
Cuts at: 1517 2313 3918 1517
Size: 796 1605 5901
Fragments arranged by size:
5901 1605 796
StyI C'CwwG_G
Cuts at: 513 1065 2022 2628 4538 4814 5082 5486 5741
Size: 552 957 606 1910 276 268 404 255
Cuts at: 5741 513
Size: 3074
Fragments arranged by size:
3074 1910 957 606 552 404 276 268
255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4084 4939 6329 6346 6499 6684 7129
Size: 1255 2504 855 1390 17 153 185 445
Cuts at: 7129 325
Size: 1498
Fragments arranged by size:
2504 1498 1390 1255 855 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3280 4014 6548
Size: 274 80 33 51 1443 1303 734 2534
Cuts at: 6548 8260 96
Size: 1712 138
Fragments arranged by size:
2534 1712 1443 1303 734 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4374 4406 8262 8294 4374
Size: 32 3856 32 4382
Fragments arranged by size:
4382 3856 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3869 1316
Size: 2553 5749
XbaI T'CTAG_A
Cuts at: 2578 5518 5638 2578
Size: 2940 120 5242
Fragments arranged by size:
5242 2940 120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3160 3573 3160
Size: 413 7889
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8302
XmaI C'CCGG_G
Cuts at: 1870 5627 1870
Size: 3757 4545
XmnI GAAnn'nnTTC
Cuts at: 3966 4397 4612 6431 3966
Size: 431 215 1819 5837
Fragments arranged by size:
5837 1819 431 215
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6110 276
Size: 53 83 186 936 4576 2468
Fragments arranged by size:
4576 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI