(Circular) (Six-base) MAPSORT of: pFN21ASDA1387.seq Check: 3692 from: 1 to: 7276 pFN21ASDA1387 7276 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5086 278 Size: 53 83 186 936 3550 2468 Fragments arranged by size: 3550 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4619 1937 Size: 2682 4594 Acc65I G'GTAC_C Cuts at: 4597 4597 Size: 7276 AclI AA'CG_TT Cuts at: 1563 5403 5776 1563 Size: 3840 373 3063 Fragments arranged by size: 3840 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2394 2607 2985 3453 5339 6723 6991 1632 Size: 762 213 378 468 1886 1384 268 1917 Fragments arranged by size: 1917 1886 1384 762 468 378 268 213 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7079 AflIII A'CryG_T Cuts at: 1203 2429 6175 7152 1203 Size: 1226 3746 977 1327 Fragments arranged by size: 3746 1327 1226 977 AgeI A'CCGG_T Cuts at: 6107 6107 Size: 7276 AhdI GACnn_n'nnGTC Cuts at: 6005 6005 Size: 7276 AleI CACnn'nnGTG Cuts at: 1166 2114 1166 Size: 948 6328 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7276 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3309 3341 3309 Size: 32 7244 AlwNI CAG_nnn'CTG Cuts at: 2792 4090 5019 6591 2792 Size: 1298 929 1572 3477 Fragments arranged by size: 3477 1572 1298 929 ApaLI G'TGCA_C Cuts at: 5333 6489 5333 Size: 1156 6120 ApoI r'AATT_y Cuts at: 1493 2310 2741 2878 3111 3197 3404 3412 3760 Size: 817 431 137 233 86 207 8 348 Cuts at: 3760 3830 3919 3944 4340 4585 4811 4963 7072 Size: 70 89 25 396 245 226 152 2109 Cuts at: 7072 1493 Size: 1697 Fragments arranged by size: 2109 1697 817 431 396 348 245 233 226 207 152 137 89 86 70 25 8 AseI AT'TA_AT Cuts at: 160 1032 2228 3270 5830 160 Size: 872 1196 1042 2560 1606 Fragments arranged by size: 2560 1606 1196 1042 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7276 AvaI C'yCGr_G Cuts at: 1870 1943 4601 1870 Size: 73 2658 4545 Fragments arranged by size: 4545 2658 73 BamHI G'GATC_C Cuts at: 4427 4606 4973 4427 Size: 179 367 6730 Fragments arranged by size: 6730 367 179 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 4210 4597 6052 618 Size: 325 217 627 303 2120 387 1455 1842 Fragments arranged by size: 2120 1842 1455 627 387 325 303 217 BanII G_rGCy'C Cuts at: 1391 2127 4595 1391 Size: 736 2468 4072 Fragments arranged by size: 4072 2468 736 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2073 3480 4055 4302 928 Size: 971 174 1407 575 247 3902 Fragments arranged by size: 3902 1407 971 575 247 174 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3996 4030 5467 5501 1903 Size: 34 2059 34 1437 34 3678 Fragments arranged by size: 3678 2059 1437 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2023 2215 5168 6378 2023 Size: 192 2953 1210 2921 Fragments arranged by size: 2953 2921 1210 192 BclI T'GATC_A Cuts at: 1548 1548 Size: 7276 BfrBI ATG'CAT Cuts at: 3083 3436 3083 Size: 353 6923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5887 136 Size: 107 122 71 631 605 4215 1525 Fragments arranged by size: 4215 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2298 7271 2298 Size: 4973 2303 BlpI GC'TnA_GC Cuts at: 2721 3240 4693 2721 Size: 519 1453 5304 Fragments arranged by size: 5304 1453 519 Bme1580I G_kGCm'C Cuts at: 1790 2100 3793 5337 6493 1790 Size: 310 1693 1544 1156 2573 Fragments arranged by size: 2573 1693 1544 1156 310 BmgBI CAC'GTC Cuts at: 1318 2101 1318 Size: 783 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5007 5965 442 Size: 416 53 83 399 24 3590 958 1753 Fragments arranged by size: 3590 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7276 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5936 1239 Size: 4697 2579 Bpu10I CC'TnA_GC Cuts at: 3309 4227 3309 Size: 918 6358 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2111 3567 5401 6266 6564 2111 Size: 1456 1834 865 298 2823 Fragments arranged by size: 2823 1834 1456 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5939 882 Size: 617 4440 2219 Fragments arranged by size: 4440 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2116 2430 4573 5067 493 Size: 711 912 314 2143 494 2702 Fragments arranged by size: 2702 2143 912 711 494 314 BsaBI GATnn'nnATC Cuts at: 3303 3633 7270 3303 Size: 330 3637 3309 Fragments arranged by size: 3637 3309 330 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5083 5465 6100 275 Size: 53 83 186 936 3550 382 635 1451 Fragments arranged by size: 3550 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5709 6107 6381 6528 6939 5709 Size: 398 274 147 411 6046 Fragments arranged by size: 6046 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 3309 3339 1178 Size: 30 165 30 687 30 1189 30 5115 Fragments arranged by size: 5115 1189 687 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2095 2135 2473 1186 Size: 909 40 338 5989 Fragments arranged by size: 5989 909 338 40 BseYI C'CCAG_C Cuts at: 6479 6975 6479 Size: 496 6780 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6703 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2086 4726 5487 6515 1540 Size: 275 186 85 2640 761 1028 2301 Fragments arranged by size: 2640 2301 1028 761 275 186 85 BsiHKAI G_wGCw'C Cuts at: 2127 2481 5337 5422 6493 2127 Size: 354 2856 85 1071 2910 Fragments arranged by size: 2910 2856 1071 354 85 BsmI GAATG_Cn' Cuts at: 4787 4880 4787 Size: 93 7183 BsmBI CGTCTCn'nnnn_ Cuts at: 2107 2107 Size: 7276 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2127 2481 3793 4595 5337 5422 Size: 399 310 27 354 1312 802 742 85 Cuts at: 5422 6493 1391 Size: 1071 2174 Fragments arranged by size: 2174 1312 1071 802 742 399 354 310 85 27 BspEI T'CCGG_A Cuts at: 6939 6939 Size: 7276 BspHI T'CATG_A Cuts at: 5165 6895 5165 Size: 1730 5546 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2430 4631 844 Size: 1586 2201 3489 Fragments arranged by size: 3489 2201 1586 BsrBI CCG'CTC Cuts at: 1394 1823 5163 1394 Size: 429 3340 3507 Fragments arranged by size: 3507 3340 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2081 5771 5945 65 Size: 1183 833 3690 174 1396 Fragments arranged by size: 3690 1396 1183 833 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2070 5920 6107 1700 Size: 110 260 3850 187 2869 Fragments arranged by size: 3850 2869 260 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7276 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7276 BssSI C'ACGA_G Cuts at: 5336 6348 5336 Size: 1012 6264 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7276 BstBI TT'CG_AA Cuts at: 2007 6080 2007 Size: 4073 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7276 BstXI CCAn_nnnn'nTGG Cuts at: 2116 2116 Size: 7276 BstYI r'GATC_y Cuts at: 1972 2298 4427 4606 4973 5358 5375 6827 6913 Size: 326 2129 179 367 385 17 1452 86 Cuts at: 6913 6925 7271 1972 Size: 12 346 1977 Fragments arranged by size: 2129 1977 1452 385 367 346 326 179 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6724 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6537 Fragments arranged by size: 6537 683 56 BtsI GCAGTG_nn' Cuts at: 4792 5586 5606 4792 Size: 794 20 6462 Fragments arranged by size: 6462 794 20 ClaI AT'CG_AT Cuts at: 1554 4016 1554 Size: 2462 4814 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7241 DraI TTT'AAA Cuts at: 3501 4580 3501 Size: 1079 6197 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6283 809 Size: 507 4967 1802 Fragments arranged by size: 4967 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4723 5612 7241 Size: 54 1425 50 275 18 2893 889 1629 Cuts at: 7241 8 Size: 43 Fragments arranged by size: 2893 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4723 1537 Size: 275 2911 4090 Fragments arranged by size: 4090 2911 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2445 2868 3574 3736 4146 4182 4438 4509 Size: 1092 423 706 162 410 36 256 71 Cuts at: 4509 5206 1353 Size: 697 3423 Fragments arranged by size: 3423 1092 706 697 423 410 256 162 71 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3823 5863 6237 6383 387 Size: 1064 138 2234 2040 374 146 1280 Fragments arranged by size: 2234 2040 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2125 2125 Size: 7276 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2394 2607 2985 3453 5339 5936 6723 Size: 393 762 213 378 468 1886 597 787 Cuts at: 6723 6991 1239 Size: 268 1524 Fragments arranged by size: 1886 1524 787 762 597 468 393 378 268 213 EcoNI CCTnn'n_nnAGG Cuts at: 2636 3312 2636 Size: 676 6600 EcoO109I rG'GnC_Cy Cuts at: 2356 2356 Size: 7276 EcoRI G'AATT_C Cuts at: 2741 3111 3760 3919 4585 4963 2741 Size: 370 649 159 666 378 5054 Fragments arranged by size: 5054 666 649 378 370 159 EcoRV GAT'ATC Cuts at: 3964 3964 Size: 7276 FspI TGC'GCA Cuts at: 5782 5782 Size: 7276 HaeII r_GCGC'y Cuts at: 1678 3324 6423 1678 Size: 1646 3099 2531 Fragments arranged by size: 3099 2531 1646 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 2157 2189 3735 Size: 32 135 32 462 32 91 32 1546 Cuts at: 3735 3767 3953 3985 5917 5949 5991 6023 1373 Size: 32 186 32 1932 32 42 32 2626 Fragments arranged by size: 2626 1932 1546 462 186 135 91 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4620 4866 669 Size: 988 281 2682 246 3079 Fragments arranged by size: 3079 2682 988 281 246 HindIII A'AGCT_T Cuts at: 748 2562 748 Size: 1814 5462 HpaI GTT'AAC Cuts at: 4866 4866 Size: 7276 KpnI G_GTAC'C Cuts at: 4601 4601 Size: 7276 MfeI C'AATT_G Cuts at: 4875 4875 Size: 7276 MluI A'CGCG_T Cuts at: 7152 7152 Size: 7276 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 2184 3500 3577 6390 6574 1305 Size: 702 177 1316 77 2813 184 2007 Fragments arranged by size: 2813 2007 1316 702 184 177 77 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5454 Fragments arranged by size: 5454 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 2604 5234 5593 5752 518 Size: 648 948 490 2630 359 159 2042 Fragments arranged by size: 2630 2042 948 648 490 359 159 MspA1I CmG'CkG Cuts at: 1606 2554 3167 3926 4692 5369 6517 6762 1606 Size: 948 613 759 766 677 1148 245 2120 Fragments arranged by size: 2120 1148 948 766 759 677 613 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6724 NdeI CA'TA_TG Cuts at: 387 387 Size: 7276 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7276 NotI GC'GGCC_GC Cuts at: 4723 4723 Size: 7276 NsiI A_TGCA'T Cuts at: 3085 3438 3085 Size: 353 6923 NspI r_CATG'y Cuts at: 1132 1229 2938 3373 3440 4634 6179 1132 Size: 97 1709 435 67 1194 1545 2229 Fragments arranged by size: 2229 1709 1545 1194 435 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7276 PciI A'CATG_T Cuts at: 6175 6175 Size: 7276 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7276 PmeI GTTT'AAAC Cuts at: 4580 4580 Size: 7276 PmlI CAC'GTG Cuts at: 2430 2430 Size: 7276 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3309 3341 5345 5377 6858 6890 3309 Size: 32 2004 32 1481 32 3695 Fragments arranged by size: 3695 2004 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2356 2356 Size: 7276 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7276 PsiI TTA'TAA Cuts at: 3837 3878 3950 4002 4846 3837 Size: 41 72 52 844 6267 Fragments arranged by size: 6267 844 72 52 41 PstI C_TGCA'G Cuts at: 830 4628 830 Size: 3798 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7276 PvuII CAG'CTG Cuts at: 2554 3167 3926 2554 Size: 613 759 5904 Fragments arranged by size: 5904 759 613 SacI G_AGCT'C Cuts at: 2127 2127 Size: 7276 SalI G'TCGA_C Cuts at: 1936 4618 1936 Size: 2682 4594 SbfI CC_TGCA'GG Cuts at: 4628 4628 Size: 7276 ScaI AGT'ACT Cuts at: 5524 7236 5524 Size: 1712 5564 SfcI C'TryA_G Cuts at: 826 1045 2994 3171 4392 4624 5759 6440 6631 Size: 219 1949 177 1221 232 1135 681 191 Cuts at: 6631 826 Size: 1471 Fragments arranged by size: 1949 1471 1221 1135 681 232 219 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7276 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7276 SmaI CCC'GGG Cuts at: 1872 4603 1872 Size: 2731 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 3582 5380 6281 6543 820 Size: 197 926 183 1456 1798 901 262 1553 Fragments arranged by size: 1798 1553 1456 926 901 262 197 183 SnaBI TAC'GTA Cuts at: 493 4573 5067 493 Size: 4080 494 2702 Fragments arranged by size: 4080 2702 494 SpeI A'CTAG_T Cuts at: 152 2405 152 Size: 2253 5023 SphI G_CATG'C Cuts at: 1132 4634 1132 Size: 3502 3774 SspI AAT'ATT Cuts at: 5 52 5200 5 Size: 47 5148 2081 Fragments arranged by size: 5148 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7276 StyI C'CwwG_G Cuts at: 513 1065 2517 3852 4146 4473 4715 513 Size: 552 1452 1335 294 327 242 3074 Fragments arranged by size: 3074 1452 1335 552 327 294 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2100 5303 5320 5473 5658 6103 325 Size: 1255 520 3203 17 153 185 445 1498 Fragments arranged by size: 3203 1498 1255 520 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 5522 7234 Size: 274 80 33 51 1443 855 2690 1712 Cuts at: 7234 96 Size: 138 Fragments arranged by size: 2690 1712 1443 855 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2090 2122 3607 3639 7236 7268 2090 Size: 32 1485 32 3597 32 2098 Fragments arranged by size: 3597 2098 1485 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4557 1316 Size: 3241 4035 XbaI T'CTAG_A Cuts at: 4612 4612 Size: 7276 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4480 4480 Size: 7276 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7276 XmaI C'CCGG_G Cuts at: 1870 4601 1870 Size: 2731 4545 XmnI GAAnn'nnTTC Cuts at: 4026 4308 5405 4026 Size: 282 1097 5897 Fragments arranged by size: 5897 1097 282 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5084 276 Size: 53 83 186 936 3550 2468 Fragments arranged by size: 3550 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI