(Circular) (Six-base) MAPSORT of: pFN21ASDA1363.seq Check: 6083 from: 1 to: 5953 pFN21ASDA1363 5953 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2205 2205 Size: 5953 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3763 278 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2079 3296 1937 Size: 142 1217 4594 Fragments arranged by size: 4594 1217 142 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2552 4016 5400 5668 1632 Size: 920 1464 1384 268 1917 Fragments arranged by size: 1917 1464 1384 920 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 3053 4852 5829 1203 Size: 1850 1799 977 1327 Fragments arranged by size: 1850 1799 1327 977 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 4682 4682 Size: 5953 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5953 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5953 AlwNI CAG_nnn'CTG Cuts at: 2980 3696 5268 2980 Size: 716 1572 3665 Fragments arranged by size: 3665 1572 716 ApaI G_GGCC'C Cuts at: 2875 2875 Size: 5953 ApaLI G'TGCA_C Cuts at: 2148 4010 5166 2148 Size: 1862 1156 2935 Fragments arranged by size: 2935 1862 1156 ApoI r'AATT_y Cuts at: 1493 2209 2496 2574 3262 3488 3640 5749 1493 Size: 716 287 78 688 226 152 2109 1697 Fragments arranged by size: 2109 1697 716 688 287 226 152 78 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 3278 1870 Size: 73 1335 4545 Fragments arranged by size: 4545 1335 73 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3274 4729 618 Size: 325 217 627 1487 1455 1842 Fragments arranged by size: 1842 1487 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2875 3272 1391 Size: 1484 397 4072 Fragments arranged by size: 4072 1484 397 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2241 928 Size: 971 342 4640 Fragments arranged by size: 4640 971 342 BbvCI CC'TCA_GC Cuts at: 2427 2427 Size: 5953 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4144 4178 1903 Size: 34 2207 34 3678 Fragments arranged by size: 3678 2207 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 1548 Size: 5953 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2057 4564 136 Size: 107 122 71 631 605 385 2507 1525 Fragments arranged by size: 2507 1525 631 605 385 122 107 71 BglII A'GATC_T Cuts at: 5948 5948 Size: 5953 BlpI GC'TnA_GC Cuts at: 3370 3370 Size: 5953 Bme1580I G_kGCm'C Cuts at: 1790 2152 2875 4014 5170 1790 Size: 362 723 1139 1156 2573 Fragments arranged by size: 2573 1156 1139 723 362 BmgBI CAC'GTC Cuts at: 1318 3054 1318 Size: 1736 4217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3684 4642 442 Size: 416 53 83 399 24 2267 958 1753 Fragments arranged by size: 2267 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4613 1239 Size: 3374 2579 Bpu10I CC'TnA_GC Cuts at: 2427 2490 2427 Size: 63 5890 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2622 4078 4943 5241 2622 Size: 1456 865 298 3334 Fragments arranged by size: 3334 1456 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4616 882 Size: 617 3117 2219 Fragments arranged by size: 3117 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3250 3744 493 Size: 711 2046 494 2702 Fragments arranged by size: 2702 2046 711 494 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2133 3760 4142 4777 Size: 53 83 186 936 600 1627 382 635 Cuts at: 4777 275 Size: 1451 Fragments arranged by size: 1627 1451 936 635 600 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3204 4386 4784 5058 5205 5616 3204 Size: 1182 398 274 147 411 3541 Fragments arranged by size: 3541 1182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5728 Fragments arranged by size: 5728 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2379 3020 1186 Size: 1193 641 4119 Fragments arranged by size: 4119 1193 641 BseYI C'CCAG_C Cuts at: 2370 5156 5652 2370 Size: 2786 496 2671 Fragments arranged by size: 2786 2671 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2831 1163 Size: 573 1095 4285 Fragments arranged by size: 4285 1095 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3403 4164 5192 1540 Size: 275 186 1402 761 1028 2301 Fragments arranged by size: 2301 1402 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2152 4014 4099 5170 2152 Size: 1862 85 1071 2935 Fragments arranged by size: 2935 1862 1071 85 BsmI GAATG_Cn' Cuts at: 2443 3464 3557 2443 Size: 1021 93 4839 Fragments arranged by size: 4839 1021 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3069 3069 Size: 5953 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2875 3272 4014 4099 5170 1391 Size: 399 362 723 397 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 723 399 397 362 85 BspEI T'CCGG_A Cuts at: 3204 5616 3204 Size: 2412 3541 BspHI T'CATG_A Cuts at: 2500 3842 5572 2500 Size: 1342 1730 2881 Fragments arranged by size: 2881 1730 1342 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2205 3308 844 Size: 1361 1103 3489 Fragments arranged by size: 3489 1361 1103 BsrBI CCG'CTC Cuts at: 1394 1823 2991 3840 1394 Size: 429 1168 849 3507 Fragments arranged by size: 3507 1168 849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2427 2823 2953 3003 4448 4622 65 Size: 1183 1179 396 130 50 1445 174 1396 Fragments arranged by size: 1445 1396 1183 1179 396 174 130 50 BsrFI r'CCGG_y Cuts at: 1700 1810 3113 4597 4784 1700 Size: 110 1303 1484 187 2869 Fragments arranged by size: 2869 1484 1303 187 110 BsrGI T'GTAC_A Cuts at: 96 2414 2932 96 Size: 2318 518 3117 Fragments arranged by size: 3117 2318 518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 4013 5025 4013 Size: 1012 4941 BstAPI GCAn_nnn'nTGC Cuts at: 794 2577 794 Size: 1783 4170 BstBI TT'CG_AA Cuts at: 2007 4757 2007 Size: 2750 3203 BstEII G'GTnAC_C Cuts at: 1184 2266 3124 1184 Size: 1082 858 4013 Fragments arranged by size: 4013 1082 858 BstXI CCAn_nnnn'nTGG Cuts at: 3103 3103 Size: 5953 BstYI r'GATC_y Cuts at: 1972 3283 3650 4035 4052 5504 5590 5602 5948 Size: 1311 367 385 17 1452 86 12 346 Cuts at: 5948 1972 Size: 1977 Fragments arranged by size: 1977 1452 1311 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2359 513 Size: 552 1294 4107 Fragments arranged by size: 4107 1294 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5214 Fragments arranged by size: 5214 683 56 BtsI GCAGTG_nn' Cuts at: 3024 3469 4263 4283 3024 Size: 445 794 20 4694 Fragments arranged by size: 4694 794 445 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2064 3400 4289 Size: 54 1425 50 275 18 234 1336 889 Cuts at: 4289 5918 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1336 889 275 234 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2322 2847 3883 1353 Size: 969 525 1036 3423 Fragments arranged by size: 3423 1036 969 525 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2983 4540 4914 5060 387 Size: 1064 138 1394 1557 374 146 1280 Fragments arranged by size: 1557 1394 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2552 4016 4613 5400 5668 1239 Size: 393 920 1464 597 787 268 1524 Fragments arranged by size: 1524 1464 920 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2312 2871 2312 Size: 559 5394 EcoRI G'AATT_C Cuts at: 3262 3640 3262 Size: 378 5575 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 2063 5100 1678 Size: 385 3037 2531 Fragments arranged by size: 3037 2531 385 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2975 3007 3118 3150 4594 Size: 32 135 32 1403 32 111 32 1444 Cuts at: 4594 4626 4668 4700 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1444 1403 135 111 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2827 3297 3543 669 Size: 988 281 889 470 246 3079 Fragments arranged by size: 3079 988 889 470 281 246 HindIII A'AGCT_T Cuts at: 748 2673 2697 748 Size: 1925 24 4004 Fragments arranged by size: 4004 1925 24 HpaI GTT'AAC Cuts at: 2827 3543 2827 Size: 716 5237 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 3552 3552 Size: 5953 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2130 5067 5251 1305 Size: 825 2937 184 2007 Fragments arranged by size: 2937 2007 825 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2066 10 Size: 54 1425 343 234 3897 Fragments arranged by size: 3897 1425 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 3911 4270 4429 518 Size: 648 2745 359 159 2042 Fragments arranged by size: 2745 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 3369 4046 5194 5439 1606 Size: 483 1280 677 1148 245 2120 Fragments arranged by size: 2120 1280 1148 677 483 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5401 NdeI CA'TA_TG Cuts at: 387 387 Size: 5953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 3311 4856 1132 Size: 97 2082 1545 2229 Fragments arranged by size: 2229 2082 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5953 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 2179 1108 Size: 1071 4882 PfoI T'CCnGG_A Cuts at: 2961 2961 Size: 5953 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 5535 5567 4022 Size: 32 1481 32 4408 Fragments arranged by size: 4408 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 3523 3523 Size: 5953 PspOMI G'GGCC_C Cuts at: 2871 2871 Size: 5953 PstI C_TGCA'G Cuts at: 830 3027 3305 830 Size: 2197 278 3478 Fragments arranged by size: 3478 2197 278 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SapI GCTCTTCn'nnn_ Cuts at: 2322 2322 Size: 5953 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 4201 5913 4201 Size: 1712 4241 SfcI C'TryA_G Cuts at: 826 1045 3023 3301 4436 5117 5308 826 Size: 219 1978 278 1135 681 191 1471 Fragments arranged by size: 1978 1471 1135 681 278 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 3280 1872 Size: 1408 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2637 4057 4958 5220 820 Size: 197 926 694 1420 901 262 1553 Fragments arranged by size: 1553 1420 926 901 694 262 197 SnaBI TAC'GTA Cuts at: 493 3250 3744 493 Size: 2757 494 2702 Fragments arranged by size: 2757 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5953 StyI C'CwwG_G Cuts at: 513 1065 2376 2620 3392 513 Size: 552 1311 244 772 3074 Fragments arranged by size: 3074 1311 772 552 244 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2285 2767 3980 3997 4150 4335 4780 Size: 1255 705 482 1213 17 153 185 445 Cuts at: 4780 325 Size: 1498 Fragments arranged by size: 1498 1255 1213 705 482 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2414 2932 4199 Size: 274 80 33 51 1443 437 518 1267 Cuts at: 4199 5911 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1267 518 437 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5913 5945 5913 Size: 32 5921 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3193 3193 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5953 XmaI C'CCGG_G Cuts at: 1870 3278 1870 Size: 1408 4545 XmnI GAAnn'nnTTC Cuts at: 4082 4082 Size: 5953 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3761 276 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI