(Circular) (Six-base) MAPSORT of: pFN21ASDA1348.seq Check: 2964 from: 1 to: 6316 pFN21ASDA1348 6316 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4126 278 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2092 3659 1937 Size: 155 1567 4594 Fragments arranged by size: 4594 1567 155 Acc65I G'GTAC_C Cuts at: 3637 3637 Size: 6316 AclI AA'CG_TT Cuts at: 1563 4443 4816 1563 Size: 2880 373 3063 Fragments arranged by size: 3063 2880 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3101 4379 5763 6031 1632 Size: 1469 1278 1384 268 1917 Fragments arranged by size: 1917 1469 1384 1278 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6119 AflIII A'CryG_T Cuts at: 1203 2939 5215 6192 1203 Size: 1736 2276 977 1327 Fragments arranged by size: 2276 1736 1327 977 AgeI A'CCGG_T Cuts at: 5147 5147 Size: 6316 AhdI GACnn_n'nnGTC Cuts at: 5045 5045 Size: 6316 AleI CACnn'nnGTG Cuts at: 1166 2630 2649 3563 1166 Size: 1464 19 914 3919 Fragments arranged by size: 3919 1464 914 19 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2845 1184 Size: 1661 4655 AlwNI CAG_nnn'CTG Cuts at: 2530 2668 2734 3382 4059 5631 2530 Size: 138 66 648 677 1572 3215 Fragments arranged by size: 3215 1572 677 648 138 66 ApaLI G'TGCA_C Cuts at: 4373 5529 4373 Size: 1156 5160 ApoI r'AATT_y Cuts at: 1493 2053 2332 2850 3625 3851 4003 6112 1493 Size: 560 279 518 775 226 152 2109 1697 Fragments arranged by size: 2109 1697 775 560 518 279 226 152 AseI AT'TA_AT Cuts at: 160 1032 2714 4870 160 Size: 872 1682 2156 1606 Fragments arranged by size: 2156 1682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 AvaI C'yCGr_G Cuts at: 1870 1943 2151 3641 1870 Size: 73 208 1490 4545 Fragments arranged by size: 4545 1490 208 73 BamHI G'GATC_C Cuts at: 3646 4013 3646 Size: 367 5949 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2155 3309 3637 5092 618 Size: 325 217 627 368 1154 328 1455 1842 Fragments arranged by size: 1842 1455 1154 627 368 328 325 217 BanII G_rGCy'C Cuts at: 1391 3372 3411 3635 1391 Size: 1981 39 224 4072 Fragments arranged by size: 4072 1981 224 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5345 BbvCI CC'TCA_GC Cuts at: 3221 3318 3221 Size: 97 6219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4507 4541 1903 Size: 34 2570 34 3678 Fragments arranged by size: 3678 2570 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4208 5418 4208 Size: 1210 5106 BclI T'GATC_A Cuts at: 1548 3444 1548 Size: 1896 4420 BfrBI ATG'CAT Cuts at: 2462 2462 Size: 6316 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4927 136 Size: 107 122 71 631 605 3255 1525 Fragments arranged by size: 3255 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6311 6311 Size: 6316 BlpI GC'TnA_GC Cuts at: 3195 3275 3733 3195 Size: 80 458 5778 Fragments arranged by size: 5778 458 80 Bme1580I G_kGCm'C Cuts at: 1790 2470 3312 4377 5533 1790 Size: 680 842 1065 1156 2573 Fragments arranged by size: 2573 1156 1065 842 680 BmgBI CAC'GTC Cuts at: 1318 3289 1318 Size: 1971 4345 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2372 3047 4047 Size: 416 53 83 399 24 955 675 1000 Cuts at: 4047 5005 442 Size: 958 1753 Fragments arranged by size: 1753 1000 958 955 675 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2089 2552 2786 3134 4976 1239 Size: 850 463 234 348 1842 2579 Fragments arranged by size: 2579 1842 850 463 348 234 Bpu10I CC'TnA_GC Cuts at: 2259 3221 3318 2259 Size: 962 97 5257 Fragments arranged by size: 5257 962 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2576 4441 5306 5604 2576 Size: 1865 865 298 3288 Fragments arranged by size: 3288 1865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3346 4979 882 Size: 617 1847 1633 2219 Fragments arranged by size: 2219 1847 1633 617 BsaAI yAC'GTr Cuts at: 493 1204 2863 2942 3613 4107 493 Size: 711 1659 79 671 494 2702 Fragments arranged by size: 2702 1659 711 671 494 79 BsaBI GATnn'nnATC Cuts at: 6310 6310 Size: 6316 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3042 4123 4505 5140 Size: 53 83 186 936 1509 1081 382 635 Cuts at: 5140 275 Size: 1451 Fragments arranged by size: 1509 1451 1081 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4749 5147 5421 5568 5979 4749 Size: 398 274 147 411 5086 Fragments arranged by size: 5086 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6091 Fragments arranged by size: 6091 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6316 BseYI C'CCAG_C Cuts at: 5519 6015 5519 Size: 496 5820 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5743 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3766 4527 5555 1540 Size: 275 186 1765 761 1028 2301 Fragments arranged by size: 2301 1765 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2538 2994 4377 4462 5533 2538 Size: 456 1383 85 1071 3321 Fragments arranged by size: 3321 1383 1071 456 85 BsmI GAATG_Cn' Cuts at: 3108 3827 3920 3108 Size: 719 93 5504 Fragments arranged by size: 5504 719 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2470 2538 2994 3312 3372 3411 3635 Size: 399 680 68 456 318 60 39 224 Cuts at: 3635 4377 4462 5533 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 680 456 399 318 224 85 68 60 39 BspEI T'CCGG_A Cuts at: 5979 5979 Size: 6316 BspHI T'CATG_A Cuts at: 4205 5935 4205 Size: 1730 4586 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3671 844 Size: 2827 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3493 4203 1394 Size: 429 1670 710 3507 Fragments arranged by size: 3507 1670 710 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2070 2078 3286 3512 4811 4985 65 Size: 1183 822 8 1208 226 1299 174 1396 Fragments arranged by size: 1396 1299 1208 1183 822 226 174 8 BsrFI r'CCGG_y Cuts at: 1700 1810 2388 4960 5147 1700 Size: 110 578 2572 187 2869 Fragments arranged by size: 2869 2572 578 187 110 BsrGI T'GTAC_A Cuts at: 96 3537 96 Size: 3441 2875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6316 BssSI C'ACGA_G Cuts at: 3413 4376 5388 3413 Size: 963 1012 4341 Fragments arranged by size: 4341 1012 963 BstAPI GCAn_nnn'nTGC Cuts at: 794 2668 3382 794 Size: 1874 714 3728 Fragments arranged by size: 3728 1874 714 BstBI TT'CG_AA Cuts at: 2007 5120 2007 Size: 3113 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6316 BstXI CCAn_nnnn'nTGG Cuts at: 2846 2846 Size: 6316 BstYI r'GATC_y Cuts at: 1972 2046 3646 4013 4398 4415 5867 5953 5965 Size: 74 1600 367 385 17 1452 86 12 Cuts at: 5965 6311 1972 Size: 346 1977 Fragments arranged by size: 1977 1600 1452 385 367 346 86 74 17 12 Bsu36I CC'TnA_GG Cuts at: 3201 3201 Size: 6316 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5764 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3518 487 Size: 683 56 2292 3285 Fragments arranged by size: 3285 2292 683 56 BtsI GCAGTG_nn' Cuts at: 2481 2511 3832 4626 4646 2481 Size: 30 1321 794 20 4151 Fragments arranged by size: 4151 1321 794 30 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6316 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6281 DraI TTT'AAA Cuts at: 3620 3620 Size: 6316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2454 5323 809 Size: 507 1138 2869 1802 Fragments arranged by size: 2869 1802 1138 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3763 4652 6281 Size: 54 1425 50 275 18 1933 889 1629 Cuts at: 6281 8 Size: 43 Fragments arranged by size: 1933 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3763 1537 Size: 275 1951 4090 Fragments arranged by size: 4090 1951 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2495 4246 1353 Size: 1142 1751 3423 Fragments arranged by size: 3423 1751 1142 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4903 5277 5423 387 Size: 1064 138 3314 374 146 1280 Fragments arranged by size: 3314 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2089 2552 2786 3101 3134 4379 4976 Size: 393 457 463 234 315 33 1245 597 Cuts at: 4976 5763 6031 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1245 787 597 463 457 393 315 268 234 33 EcoNI CCTnn'n_nnAGG Cuts at: 3317 3317 Size: 6316 EcoO109I rG'GnC_Cy Cuts at: 2764 3060 3136 3225 2764 Size: 296 76 89 5855 Fragments arranged by size: 5855 296 89 76 EcoRI G'AATT_C Cuts at: 2850 3625 4003 2850 Size: 775 378 5163 Fragments arranged by size: 5163 775 378 EcoRV GAT'ATC Cuts at: 3550 3550 Size: 6316 FspI TGC'GCA Cuts at: 4822 4822 Size: 6316 HaeII r_GCGC'y Cuts at: 1678 5463 1678 Size: 3785 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2612 2644 3441 3473 4957 Size: 32 135 32 1040 32 797 32 1484 Cuts at: 4957 4989 5031 5063 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1484 1040 797 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3660 3906 669 Size: 988 281 1722 246 3079 Fragments arranged by size: 3079 1722 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6316 HpaI GTT'AAC Cuts at: 3906 3906 Size: 6316 KpnI G_GTAC'C Cuts at: 3641 3641 Size: 6316 MfeI C'AATT_G Cuts at: 3915 3915 Size: 6316 MluI A'CGCG_T Cuts at: 6192 6192 Size: 6316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5430 5614 1305 Size: 4125 184 2007 Fragments arranged by size: 4125 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4494 Fragments arranged by size: 4494 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2463 2630 2649 2687 2844 3563 4274 Size: 648 1297 167 19 38 157 719 711 Cuts at: 4274 4633 4792 518 Size: 359 159 2042 Fragments arranged by size: 2042 1297 719 711 648 359 167 159 157 38 19 MspA1I CmG'CkG Cuts at: 1606 2362 3079 3405 3732 4409 5557 5802 1606 Size: 756 717 326 327 677 1148 245 2120 Fragments arranged by size: 2120 1148 756 717 677 327 326 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5764 NdeI CA'TA_TG Cuts at: 387 387 Size: 6316 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6316 NotI GC'GGCC_GC Cuts at: 3763 3763 Size: 6316 NsiI A_TGCA'T Cuts at: 2464 2464 Size: 6316 NspI r_CATG'y Cuts at: 1132 1229 2466 2920 3274 3455 3674 5219 1132 Size: 97 1237 454 354 181 219 1545 2229 Fragments arranged by size: 2229 1545 1237 454 354 219 181 97 PasI CC'CwG_GG Cuts at: 1348 2600 1348 Size: 1252 5064 PciI A'CATG_T Cuts at: 5215 5215 Size: 6316 PflMI CCAn_nnn'nTGG Cuts at: 1108 2186 2530 2734 1108 Size: 1078 344 204 4690 Fragments arranged by size: 4690 1078 344 204 PmeI GTTT'AAAC Cuts at: 3620 3620 Size: 6316 PmlI CAC'GTG Cuts at: 2863 2942 2863 Size: 79 6237 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4385 4417 5898 5930 4385 Size: 32 1481 32 4771 Fragments arranged by size: 4771 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6316 PsiI TTA'TAA Cuts at: 3886 3886 Size: 6316 PstI C_TGCA'G Cuts at: 830 3059 3393 3668 830 Size: 2229 334 275 3478 Fragments arranged by size: 3478 2229 334 275 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6316 PvuII CAG'CTG Cuts at: 2362 3079 2362 Size: 717 5599 SalI G'TCGA_C Cuts at: 1936 3658 1936 Size: 1722 4594 SapI GCTCTTCn'nnn_ Cuts at: 2495 2495 Size: 6316 SbfI CC_TGCA'GG Cuts at: 3059 3668 3059 Size: 609 5707 ScaI AGT'ACT Cuts at: 2032 4564 6276 2032 Size: 2532 1712 2072 Fragments arranged by size: 2532 2072 1712 SfcI C'TryA_G Cuts at: 826 1045 2209 3055 3389 3664 4799 5480 5671 Size: 219 1164 846 334 275 1135 681 191 Cuts at: 5671 826 Size: 1471 Fragments arranged by size: 1471 1164 1135 846 681 334 275 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6316 SmaI CCC'GGG Cuts at: 1872 2153 3643 1872 Size: 281 1490 4545 Fragments arranged by size: 4545 1490 281 SmlI C'TyrA_G Cuts at: 820 1017 1943 2591 4420 5321 5583 820 Size: 197 926 648 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 648 262 197 SnaBI TAC'GTA Cuts at: 493 3613 4107 493 Size: 3120 494 2702 Fragments arranged by size: 3120 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6316 SphI G_CATG'C Cuts at: 1132 3674 1132 Size: 2542 3774 SspI AAT'ATT Cuts at: 5 52 4240 5 Size: 47 4188 2081 Fragments arranged by size: 4188 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6316 StyI C'CwwG_G Cuts at: 513 1065 2893 3755 513 Size: 552 1828 862 3074 Fragments arranged by size: 3074 1828 862 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2176 2613 4343 4360 4513 4698 5143 Size: 1255 596 437 1730 17 153 185 445 Cuts at: 5143 325 Size: 1498 Fragments arranged by size: 1730 1498 1255 596 445 437 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2030 2718 3537 Size: 274 80 33 51 1443 53 688 819 Cuts at: 3537 4562 6274 96 Size: 1025 1712 138 Fragments arranged by size: 1712 1443 1025 819 688 274 138 80 53 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6276 6308 6276 Size: 32 6284 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6316 XbaI T'CTAG_A Cuts at: 3652 3652 Size: 6316 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2909 2377 Size: 532 5784 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6316 XmaI C'CCGG_G Cuts at: 1870 2151 3641 1870 Size: 281 1490 4545 Fragments arranged by size: 4545 1490 281 XmnI GAAnn'nnTTC Cuts at: 2277 4445 2277 Size: 2168 4148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4124 276 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI