(Circular) (Six-base) MAPSORT of: pFN21ASDA1281.seq Check: 516 from: 1 to: 9265 pFN21ASDA1281 9265 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6408 6496 6408 Size: 88 9177 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7075 278 Size: 53 83 186 936 5539 2468 Fragments arranged by size: 5539 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5703 5760 6608 1937 Size: 3766 57 848 4594 Fragments arranged by size: 4594 3766 848 57 Acc65I G'GTAC_C Cuts at: 3735 6586 3735 Size: 2851 6414 AclI AA'CG_TT Cuts at: 1563 7392 7765 1563 Size: 5829 373 3063 Fragments arranged by size: 5829 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2660 5585 7328 8712 8980 1632 Size: 1028 2925 1743 1384 268 1917 Fragments arranged by size: 2925 1917 1743 1384 1028 268 AfeI AGC'GCT Cuts at: 2478 2478 Size: 9265 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9068 AflIII A'CryG_T Cuts at: 1203 3083 8164 9141 1203 Size: 1880 5081 977 1327 Fragments arranged by size: 5081 1880 1327 977 AgeI A'CCGG_T Cuts at: 3037 8096 3037 Size: 5059 4206 AhdI GACnn_n'nnGTC Cuts at: 4882 5296 7994 4882 Size: 414 2698 6153 Fragments arranged by size: 6153 2698 414 AleI CACnn'nnGTG Cuts at: 1166 4748 1166 Size: 3582 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3862 5082 6528 1184 Size: 2678 1220 1446 3921 Fragments arranged by size: 3921 2678 1446 1220 AlwNI CAG_nnn'CTG Cuts at: 5362 7008 8580 5362 Size: 1646 1572 6047 Fragments arranged by size: 6047 1646 1572 ApaI G_GGCC'C Cuts at: 3126 3265 3126 Size: 139 9126 ApaLI G'TGCA_C Cuts at: 7322 8478 7322 Size: 1156 8109 ApoI r'AATT_y Cuts at: 1493 2116 2162 2273 2308 2470 3148 3598 3997 Size: 623 46 111 35 162 678 450 399 Cuts at: 3997 4150 6574 6800 6952 9061 1493 Size: 153 2424 226 152 2109 1697 Fragments arranged by size: 2424 2109 1697 678 623 450 399 226 162 153 152 111 46 35 AseI AT'TA_AT Cuts at: 160 1032 4136 7819 160 Size: 872 3104 3683 1606 Fragments arranged by size: 3683 3104 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9265 AvaI C'yCGr_G Cuts at: 1870 1943 2405 3355 4726 5713 5997 6448 6590 Size: 73 462 950 1371 987 284 451 142 Cuts at: 6590 1870 Size: 4545 Fragments arranged by size: 4545 1371 987 950 462 451 284 142 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3852 3885 3852 Size: 33 9232 BamHI G'GATC_C Cuts at: 2059 6595 6962 2059 Size: 4536 367 4362 Fragments arranged by size: 4536 4362 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2445 2621 2721 3735 3892 Size: 325 217 627 658 176 100 1014 157 Cuts at: 3892 4762 4771 5066 6396 6586 8041 618 Size: 870 9 295 1330 190 1455 1842 Fragments arranged by size: 1842 1455 1330 1014 870 658 627 325 295 217 190 176 157 100 9 BanII G_rGCy'C Cuts at: 1391 2768 2847 3126 3265 3362 4416 5283 5421 Size: 1377 79 279 139 97 1054 867 138 Cuts at: 5421 5907 6584 1391 Size: 486 677 4072 Fragments arranged by size: 4072 1377 1054 867 677 486 279 139 138 97 79 BbeI G_GCGC'C Cuts at: 2449 2625 2449 Size: 176 9089 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 8294 BbvCI CC'TCA_GC Cuts at: 3483 4665 5205 3483 Size: 1182 540 7543 Fragments arranged by size: 7543 1182 540 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7456 7490 1903 Size: 34 5519 34 3678 Fragments arranged by size: 5519 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7157 8367 7157 Size: 1210 8055 BclI T'GATC_A Cuts at: 1548 5220 1548 Size: 3672 5593 BfrBI ATG'CAT Cuts at: 5273 5273 Size: 9265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7876 136 Size: 107 122 71 631 605 6204 1525 Fragments arranged by size: 6204 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3804 9260 3804 Size: 5456 3809 BlpI GC'TnA_GC Cuts at: 5622 6339 6682 5622 Size: 717 343 8205 Fragments arranged by size: 8205 717 343 Bme1580I G_kGCm'C Cuts at: 1790 2724 2786 2833 3126 3265 4228 5071 5868 Size: 934 62 47 293 139 963 843 797 Cuts at: 5868 7326 8482 1790 Size: 1458 1156 2573 Fragments arranged by size: 2573 1458 1156 963 934 843 797 293 139 62 47 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2796 3268 4317 Size: 416 53 83 399 24 1379 472 1049 Cuts at: 4317 5397 6996 7954 442 Size: 1080 1599 958 1753 Fragments arranged by size: 1753 1599 1379 1080 1049 958 472 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9265 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5859 5859 Size: 9265 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2215 2472 3320 3541 3654 3797 6144 6263 Size: 976 257 848 221 113 143 2347 119 Cuts at: 6263 7925 1239 Size: 1662 2579 Fragments arranged by size: 2579 2347 1662 976 848 257 221 143 119 113 Bpu10I CC'TnA_GC Cuts at: 2257 3483 4665 5205 2257 Size: 1226 1182 540 6317 Fragments arranged by size: 6317 1226 1182 540 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3609 7390 8255 8553 3609 Size: 3781 865 298 4321 Fragments arranged by size: 4321 3781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 7928 882 Size: 617 816 5613 2219 Fragments arranged by size: 5613 2219 816 617 BsaAI yAC'GTr Cuts at: 493 1204 3207 3226 6562 7056 493 Size: 711 2003 19 3336 494 2702 Fragments arranged by size: 3336 2702 2003 711 494 19 BsaBI GATnn'nnATC Cuts at: 9259 9259 Size: 9265 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2446 2622 7072 7454 Size: 53 83 186 936 913 176 4450 382 Cuts at: 7454 8089 275 Size: 635 1451 Fragments arranged by size: 4450 1451 936 913 635 382 186 176 83 53 BsaWI w'CCGG_w Cuts at: 3037 5500 7698 8096 8370 8517 8928 3037 Size: 2463 2198 398 274 147 411 3374 Fragments arranged by size: 3374 2463 2198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3539 3569 1178 Size: 30 165 30 2136 30 6874 Fragments arranged by size: 6874 2136 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 2894 2897 2900 2903 5409 5570 5915 Size: 1417 291 3 3 3 2506 161 345 Cuts at: 5915 5957 6010 1186 Size: 42 53 4441 Fragments arranged by size: 4441 2506 1417 345 291 161 53 42 3 3 3 BseYI C'CCAG_C Cuts at: 2479 3989 4246 4429 4528 4703 5434 5867 6092 Size: 1510 257 183 99 175 731 433 225 Cuts at: 6092 8468 8964 2479 Size: 2376 496 2780 Fragments arranged by size: 2780 2376 1510 731 496 433 257 225 183 175 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3229 6386 1163 Size: 573 1493 3157 4042 Fragments arranged by size: 4042 3157 1493 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6715 7476 8504 1540 Size: 275 186 4714 761 1028 2301 Fragments arranged by size: 4714 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5096 5421 7326 7411 8482 5096 Size: 325 1905 85 1071 5879 Fragments arranged by size: 5879 1905 1071 325 85 BsiWI C'GTAC_G Cuts at: 3222 3222 Size: 9265 BsmI GAATG_Cn' Cuts at: 3851 4742 4957 6776 6869 3851 Size: 891 215 1819 93 6247 Fragments arranged by size: 6247 1819 891 215 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4493 4493 Size: 9265 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2724 2768 2786 2833 2847 3126 3265 Size: 399 934 44 18 47 14 279 139 Cuts at: 3265 3362 4228 4416 5071 5096 5283 5421 5868 Size: 97 866 188 655 25 187 138 447 Cuts at: 5868 5907 6584 7326 7411 8482 1391 Size: 39 677 742 85 1071 2174 Fragments arranged by size: 2174 1071 934 866 742 677 655 447 399 279 188 187 139 138 97 85 47 44 39 25 18 14 BspEI T'CCGG_A Cuts at: 5500 8928 5500 Size: 3428 5837 BspHI T'CATG_A Cuts at: 7154 8884 7154 Size: 1730 7535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2679 4872 5050 6408 6496 6620 844 Size: 1835 2193 178 1358 88 124 3489 Fragments arranged by size: 3489 2193 1835 1358 178 124 88 BsrBI CCG'CTC Cuts at: 1394 1823 6343 7152 1394 Size: 429 4520 809 3507 Fragments arranged by size: 4520 3507 809 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2755 3964 4233 7760 7934 65 Size: 1183 1507 1209 269 3527 174 1396 Fragments arranged by size: 3527 1507 1396 1209 1183 269 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3037 3895 7909 8096 1700 Size: 110 1227 858 4014 187 2869 Fragments arranged by size: 4014 2869 1227 858 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9265 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9265 BssSI C'ACGA_G Cuts at: 7325 8337 7325 Size: 1012 8253 BstAPI GCAn_nnn'nTGC Cuts at: 794 2671 3341 794 Size: 1877 670 6718 Fragments arranged by size: 6718 1877 670 BstBI TT'CG_AA Cuts at: 2007 8069 2007 Size: 6062 3203 BstEII G'GTnAC_C Cuts at: 1184 6434 1184 Size: 5250 4015 BstXI CCAn_nnnn'nTGG Cuts at: 2690 4405 5776 2690 Size: 1715 1371 6179 Fragments arranged by size: 6179 1715 1371 BstYI r'GATC_y Cuts at: 1972 2059 3804 6595 6962 7347 7364 8816 8902 Size: 87 1745 2791 367 385 17 1452 86 Cuts at: 8902 8914 9260 1972 Size: 12 346 1977 Fragments arranged by size: 2791 1977 1745 1452 385 367 346 87 86 17 12 BstZ17I GTA'TAC Cuts at: 5761 5761 Size: 9265 Bsu36I CC'TnA_GG Cuts at: 3731 3731 Size: 9265 BtgI C'CryG_G Cuts at: 513 1065 2556 513 Size: 552 1491 7222 Fragments arranged by size: 7222 1491 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2106 487 Size: 683 56 880 7646 Fragments arranged by size: 7646 880 683 56 BtsI GCAGTG_nn' Cuts at: 2701 2773 3856 4281 6781 7575 7595 2701 Size: 72 1083 425 2500 794 20 4371 Fragments arranged by size: 4371 2500 1083 794 425 72 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9265 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2197 2232 2737 2772 529 Size: 35 1633 35 505 35 7022 Fragments arranged by size: 7022 1633 505 35 35 35 DraI TTT'AAA Cuts at: 4584 5722 6569 4584 Size: 1138 847 7280 Fragments arranged by size: 7280 1138 847 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8272 809 Size: 507 6956 1802 Fragments arranged by size: 6956 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4650 5263 5307 Size: 54 1425 50 275 18 2820 613 44 Cuts at: 5307 5472 6388 6712 7601 9230 8 Size: 165 916 324 889 1629 43 Fragments arranged by size: 2820 1629 1425 916 889 613 324 275 165 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6712 1537 Size: 275 4900 4090 Fragments arranged by size: 4900 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2871 3323 3670 6198 6547 7195 1353 Size: 1518 452 347 2528 349 648 3423 Fragments arranged by size: 3423 2528 1518 648 452 349 347 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7852 8226 8372 387 Size: 1064 138 6263 374 146 1280 Fragments arranged by size: 6263 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5419 5419 Size: 9265 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2215 2472 2660 3320 3541 3654 3797 Size: 393 583 257 188 660 221 113 143 Cuts at: 3797 5585 6144 6263 7328 7925 8712 8980 1239 Size: 1788 559 119 1065 597 787 268 1524 Fragments arranged by size: 1788 1524 1065 787 660 597 583 559 393 268 257 221 188 143 119 113 EcoNI CCTnn'n_nnAGG Cuts at: 2675 4713 6468 2675 Size: 2038 1755 5472 Fragments arranged by size: 5472 2038 1755 EcoO109I rG'GnC_Cy Cuts at: 2707 3123 3262 3927 4015 2707 Size: 416 139 665 88 7957 Fragments arranged by size: 7957 665 416 139 88 EcoRI G'AATT_C Cuts at: 2308 2470 3997 6574 6952 2308 Size: 162 1527 2577 378 4621 Fragments arranged by size: 4621 2577 1527 378 162 EcoRV GAT'ATC Cuts at: 3490 3490 Size: 9265 FspI TGC'GCA Cuts at: 7771 7771 Size: 9265 HaeII r_GCGC'y Cuts at: 1678 2449 2480 2625 2957 4725 8412 1678 Size: 771 31 145 332 1768 3687 2531 Fragments arranged by size: 3687 2531 1768 771 332 145 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3539 3571 3791 3823 4064 Size: 32 135 32 1967 32 220 32 241 Cuts at: 4064 4096 4401 4433 4868 4900 5282 5314 5696 Size: 32 305 32 435 32 382 32 382 Cuts at: 5696 5728 5918 5950 6000 6022 6032 6054 6362 Size: 32 190 32 50 22 10 22 308 Cuts at: 6362 6394 7906 7938 7980 8012 1373 Size: 32 1512 32 42 32 2626 Fragments arranged by size: 2626 1967 1512 435 382 382 308 305 241 220 190 135 50 42 32 32 32 32 32 32 32 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 2932 4788 5886 6238 6609 6855 Size: 988 281 994 1856 1098 352 371 246 Cuts at: 6855 669 Size: 3079 Fragments arranged by size: 3079 1856 1098 994 988 371 352 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 9265 HpaI GTT'AAC Cuts at: 5886 6855 5886 Size: 969 8296 KasI G'GCGC_C Cuts at: 2445 2621 2445 Size: 176 9089 KpnI G_GTAC'C Cuts at: 3739 6590 3739 Size: 2851 6414 MfeI C'AATT_G Cuts at: 6864 6864 Size: 9265 MluI A'CGCG_T Cuts at: 9141 9141 Size: 9265 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2092 3073 4077 4608 4866 8379 8563 1305 Size: 787 981 1004 531 258 3513 184 2007 Fragments arranged by size: 3513 2007 1004 981 787 531 258 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4652 5265 5309 10 Size: 54 1425 343 2820 613 44 3966 Fragments arranged by size: 3966 2820 1425 613 343 54 44 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2688 2727 3844 4403 4421 4748 Size: 648 861 661 39 1117 559 18 327 Cuts at: 4748 5019 5754 7223 7582 7741 518 Size: 271 735 1469 359 159 2042 Fragments arranged by size: 2042 1469 1117 861 735 661 648 559 359 327 271 159 39 18 MspA1I CmG'CkG Cuts at: 1606 2499 2508 2558 2807 3453 4814 5078 6338 Size: 893 9 50 249 646 1361 264 1260 Cuts at: 6338 6394 6681 7358 8506 8751 1606 Size: 56 287 677 1148 245 2120 Fragments arranged by size: 2120 1361 1260 1148 893 677 646 287 264 249 245 56 50 9 NarI GG'CG_CC Cuts at: 2446 2622 2446 Size: 176 9089 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8713 NdeI CA'TA_TG Cuts at: 387 5275 387 Size: 4888 4377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9265 NotI GC'GGCC_GC Cuts at: 6712 6712 Size: 9265 NsiI A_TGCA'T Cuts at: 5275 5275 Size: 9265 NspI r_CATG'y Cuts at: 1132 1229 3087 4784 5050 6065 6290 6623 8168 Size: 97 1858 1697 266 1015 225 333 1545 Cuts at: 8168 1132 Size: 2229 Fragments arranged by size: 2229 1858 1697 1545 1015 333 266 225 97 PasI CC'CwG_GG Cuts at: 1348 2302 5054 1348 Size: 954 2752 5559 Fragments arranged by size: 5559 2752 954 PciI A'CATG_T Cuts at: 3083 8164 3083 Size: 5081 4184 PflMI CCAn_nnn'nTGG Cuts at: 1108 5362 1108 Size: 4254 5011 PfoI T'CCnGG_A Cuts at: 2651 6160 6541 2651 Size: 3509 381 5375 Fragments arranged by size: 5375 3509 381 PmeI GTTT'AAAC Cuts at: 6569 6569 Size: 9265 PmlI CAC'GTG Cuts at: 3207 3207 Size: 9265 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 7334 7366 8847 8879 3545 Size: 32 3757 32 1481 32 3931 Fragments arranged by size: 3931 3757 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3927 3927 Size: 9265 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9265 PsiI TTA'TAA Cuts at: 6835 6835 Size: 9265 PspOMI G'GGCC_C Cuts at: 3122 3261 3122 Size: 139 9126 PspXI vC'TCGA_Gb Cuts at: 4726 4726 Size: 9265 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3206 3238 3206 Size: 32 9233 PstI C_TGCA'G Cuts at: 830 2047 2812 3396 5077 6617 830 Size: 1217 765 584 1681 1540 3478 Fragments arranged by size: 3478 1681 1540 1217 765 584 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9265 PvuII CAG'CTG Cuts at: 4814 5078 6338 4814 Size: 264 1260 7741 Fragments arranged by size: 7741 1260 264 SacI G_AGCT'C Cuts at: 5421 5421 Size: 9265 SacII CC_GC'GG Cuts at: 2559 2559 Size: 9265 SalI G'TCGA_C Cuts at: 1936 6607 1936 Size: 4671 4594 SanDI GG'GwC_CC Cuts at: 3927 3927 Size: 9265 SapI GCTCTTCn'nnn_ Cuts at: 3670 3670 Size: 9265 SbfI CC_TGCA'GG Cuts at: 6617 6617 Size: 9265 ScaI AGT'ACT Cuts at: 5345 6056 7513 9225 5345 Size: 711 1457 1712 5385 Fragments arranged by size: 5385 1712 1457 711 SfcI C'TryA_G Cuts at: 826 1045 2043 2808 3392 4968 5073 5323 5658 Size: 219 998 765 584 1576 105 250 335 Cuts at: 5658 6333 6613 7748 8429 8620 826 Size: 675 280 1135 681 191 1471 Fragments arranged by size: 1576 1471 1135 998 765 681 675 584 335 280 250 219 191 105 SfoI GGC'GCC Cuts at: 2447 2623 2447 Size: 176 9089 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9265 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9265 SmaI CCC'GGG Cuts at: 1872 3357 6450 6592 1872 Size: 1485 3093 142 4545 Fragments arranged by size: 4545 3093 1485 142 SmlI C'TyrA_G Cuts at: 820 1017 1943 3624 4726 5713 7369 8270 8532 Size: 197 926 1681 1102 987 1656 901 262 Cuts at: 8532 820 Size: 1553 Fragments arranged by size: 1681 1656 1553 1102 987 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6562 7056 493 Size: 6069 494 2702 Fragments arranged by size: 6069 2702 494 SpeI A'CTAG_T Cuts at: 152 2192 152 Size: 2040 7225 SphI G_CATG'C Cuts at: 1132 6065 6290 6623 1132 Size: 4933 225 333 3774 Fragments arranged by size: 4933 3774 333 225 SspI AAT'ATT Cuts at: 5 52 3028 7189 5 Size: 47 2976 4161 2081 Fragments arranged by size: 4161 2976 2081 47 StuI AGG'CCT Cuts at: 1517 2291 1517 Size: 774 8491 StyI C'CwwG_G Cuts at: 513 1065 2208 2440 3655 4548 6704 513 Size: 552 1143 232 1215 893 2156 3074 Fragments arranged by size: 3074 2156 1215 1143 893 552 232 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3062 3708 6040 6434 7292 7309 7462 Size: 1255 1482 646 2332 394 858 17 153 Cuts at: 7462 7647 8092 325 Size: 185 445 1498 Fragments arranged by size: 2332 1498 1482 1255 858 646 445 394 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3711 5343 6054 Size: 274 80 33 51 1443 1734 1632 711 Cuts at: 6054 7511 9223 96 Size: 1457 1712 138 Fragments arranged by size: 1734 1712 1632 1457 1443 711 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2040 2072 5994 6026 9225 9257 2040 Size: 32 3922 32 3199 32 2048 Fragments arranged by size: 3922 3199 2048 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9265 XbaI T'CTAG_A Cuts at: 6601 6601 Size: 9265 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2447 2693 3148 5273 6422 2447 Size: 246 455 2125 1149 5290 Fragments arranged by size: 5290 2125 1149 455 246 XhoI C'TCGA_G Cuts at: 1943 4726 5713 1943 Size: 2783 987 5495 Fragments arranged by size: 5495 2783 987 XmaI C'CCGG_G Cuts at: 1870 3355 6448 6590 1870 Size: 1485 3093 142 4545 Fragments arranged by size: 4545 3093 1485 142 XmnI GAAnn'nnTTC Cuts at: 7394 7394 Size: 9265 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7073 276 Size: 53 83 186 936 5539 2468 Fragments arranged by size: 5539 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII DraIII FalI FseI FspAI NaeI NgoMIV NruI PacI RsrII SexAI SfiI SrfI SwaI