Restriction map for
pFN21ASDA1279
(Linear) (Six-base) MAPSORT of: KIAA1279.seq Check: 5243 from: 1 to: 1863
KIAA1279.orf
With 182 enzymes: SgfI *
October 24, 2006 15:16 ..
AccI GT'mk_AC
Cuts at: 0 650 1376 1863
Size: 650 726 487
Fragments arranged by size:
726 650 487
Acc65I G'GTAC_C
Cuts at: 0 380 1863
Size: 380 1483
AclI AA'CG_TT
Cuts at: 0 8 1863
Size: 8 1855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 899 905 1561 1863
Size: 587 312 6 656 302
Fragments arranged by size:
656 587 312 302 6
AgeI A'CCGG_T
Cuts at: 0 1099 1863
Size: 1099 764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 42 1863
Size: 42 1821
AlwNI CAG_nnn'CTG
Cuts at: 0 660 812 1863
Size: 660 152 1051
Fragments arranged by size:
1051 660 152
ApaI G_GGCC'C
Cuts at: 0 257 1863
Size: 257 1606
ApoI r'AATT_y
Cuts at: 0 34 1701 1729 1863
Size: 34 1667 28 134
Fragments arranged by size:
1667 134 34 28
AvaI C'yCGr_G
Cuts at: 0 116 198 247 301 1863
Size: 116 82 49 54 1562
Fragments arranged by size:
1562 116 82 54 49
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 420 453 1863
Size: 420 33 1410
Fragments arranged by size:
1410 420 33
BamHI G'GATC_C
Cuts at: 0 1488 1863
Size: 1488 375
BanI G'GyrC_C
Cuts at: 0 202 380 1863
Size: 202 178 1483
Fragments arranged by size:
1483 202 178
BanII G_rGCy'C
Cuts at: 0 248 257 1367 1863
Size: 248 9 1110 496
Fragments arranged by size:
1110 496 248 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 875 1807 1863
Size: 875 932 56
Fragments arranged by size:
932 875 56
BbvCI CC'TCA_GC
Cuts at: 0 233 1863
Size: 233 1630
BfrBI ATG'CAT
Cuts at: 0 1338 1863
Size: 1338 525
BglI GCCn_nnn'nGGC
Cuts at: 0 252 1863
Size: 252 1611
BglII A'GATC_T
Cuts at: 0 854 1863
Size: 854 1009
Bme1580I G_kGCm'C
Cuts at: 0 257 1863
Size: 257 1606
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 554 1863
Size: 554 1309
Bpu10I CC'TnA_GC
Cuts at: 0 233 1483 1595 1863
Size: 233 1250 112 268
Fragments arranged by size:
1250 268 233 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 736 1170 1863
Size: 629 107 434 693
Fragments arranged by size:
693 629 434 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 1146 1568 1863
Size: 1146 422 295
Fragments arranged by size:
1146 422 295
BsaWI w'CCGG_w
Cuts at: 0 81 1099 1863
Size: 81 1018 764
Fragments arranged by size:
1018 764 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 1312 1342 1863
Size: 316 30 966 30 521
Fragments arranged by size:
966 521 316 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 340 358 527 1075 1863
Size: 340 18 169 548 788
Fragments arranged by size:
788 548 340 169 18
BseYI C'CCAG_C
Cuts at: 0 263 1863
Size: 263 1600
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1108 1863
Size: 455 653 755
Fragments arranged by size:
755 653 455
BsiHKAI G_wGCw'C
Cuts at: 0 350 722 1284 1863
Size: 350 372 562 579
Fragments arranged by size:
579 562 372 350
BsmI GAATG_Cn'
Cuts at: 0 975 1070 1300 1863
Size: 975 95 230 563
Fragments arranged by size:
975 563 230 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1317 1660 1863
Size: 1317 343 203
Fragments arranged by size:
1317 343 203
Bsp1286I G_dGCh'C
Cuts at: 0 248 257 350 722 1284 1367 1863
Size: 248 9 93 372 562 83 496
Fragments arranged by size:
562 496 372 248 93 83 9
BspEI T'CCGG_A
Cuts at: 0 81 1863
Size: 81 1782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 368 1863
Size: 368 1495
BsrBI CCG'CTC
Cuts at: 0 177 271 1863
Size: 177 94 1592
Fragments arranged by size:
1592 177 94
BsrDI GCAATG_nn'
Cuts at: 0 1469 1863
Size: 1469 394
BsrFI r'CCGG_y
Cuts at: 0 205 228 383 1099 1863
Size: 205 23 155 716 764
Fragments arranged by size:
764 716 205 155 23
BstEII G'GTnAC_C
Cuts at: 0 208 1863
Size: 208 1655
BstYI r'GATC_y
Cuts at: 0 854 1415 1488 1680 1863
Size: 854 561 73 192 183
Fragments arranged by size:
854 561 192 183 73
Bsu36I CC'TnA_GG
Cuts at: 0 161 182 1760 1863
Size: 161 21 1578 103
Fragments arranged by size:
1578 161 103 21
BtgI C'CryG_G
Cuts at: 0 13 1863
Size: 13 1850
DraIII CAC_nnn'GTG
Cuts at: 0 354 1863
Size: 354 1509
EarI CTCTTCn'nnn_
Cuts at: 0 125 566 1127 1561 1863
Size: 125 441 561 434 302
Fragments arranged by size:
561 441 434 302 125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 899 905 1561 1863
Size: 587 312 6 656 302
Fragments arranged by size:
656 587 312 302 6
EcoO109I rG'GnC_Cy
Cuts at: 0 253 1863
Size: 253 1610
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 595 810 1633 1863
Size: 595 215 823 230
Fragments arranged by size:
823 595 230 215
FspI TGC'GCA
Cuts at: 0 376 1863
Size: 376 1487
HaeII r_GCGC'y
Cuts at: 0 49 116 124 145 1863
Size: 49 67 8 21 1718
Fragments arranged by size:
1718 67 49 21 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 388 420 1006 1038 1105 1137
Size: 316 32 40 32 586 32 67 32
Cuts at: 1137 1797 1829 1863
Size: 660 32 34
Fragments arranged by size:
660 586 316 67 40 34 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1407 1863
Size: 1407 456
KpnI G_GTAC'C
Cuts at: 0 384 1863
Size: 384 1479
MspA1I CmG'CkG
Cuts at: 0 823 1863
Size: 823 1040
NaeI GCC'GGC
Cuts at: 0 230 1863
Size: 230 1633
NgoMIV G'CCGG_C
Cuts at: 0 228 1863
Size: 228 1635
NsiI A_TGCA'T
Cuts at: 0 1340 1863
Size: 1340 523
NspI r_CATG'y
Cuts at: 0 1438 1863
Size: 1438 425
PasI CC'CwG_GG
Cuts at: 0 219 1863
Size: 219 1644
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 660 1863
Size: 219 441 1203
Fragments arranged by size:
1203 441 219
PspOMI G'GGCC_C
Cuts at: 0 253 1863
Size: 253 1610
PstI C_TGCA'G
Cuts at: 0 1128 1680 1863
Size: 1128 552 183
Fragments arranged by size:
1128 552 183
PvuII CAG'CTG
Cuts at: 0 823 1863
Size: 823 1040
SapI GCTCTTCn'nnn_
Cuts at: 0 1561 1863
Size: 1561 302
ScaI AGT'ACT
Cuts at: 0 1546 1863
Size: 1546 317
SexAI A'CCwGG_T
Cuts at: 0 350 1863
Size: 350 1513
SfcI C'TryA_G
Cuts at: 0 737 1124 1373 1676 1863
Size: 737 387 249 303 187
Fragments arranged by size:
737 387 303 249 187
SmaI CCC'GGG
Cuts at: 0 118 200 1863
Size: 118 82 1663
Fragments arranged by size:
1663 118 82
SmlI C'TyrA_G
Cuts at: 0 644 715 1149 1863
Size: 644 71 434 714
Fragments arranged by size:
714 644 434 71
SrfI GCCC'GGGC
Cuts at: 0 118 1863
Size: 118 1745
TatI w'GTAC_w
Cuts at: 0 697 1544 1863
Size: 697 847 319
Fragments arranged by size:
847 697 319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1312 1344 1863
Size: 1312 32 519
Fragments arranged by size:
1312 519 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1692 1863
Size: 1692 171
XmaI C'CCGG_G
Cuts at: 0 116 198 1863
Size: 116 82 1665
Fragments arranged by size:
1665 116 82
XmnI GAAnn'nnTTC
Cuts at: 0 1070 1613 1863
Size: 1070 543 250
Fragments arranged by size:
1070 543 250
Enzymes that do cut:
AccI Acc65I AclI AcuI AgeI AlfI AlwNI ApaI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BfrBI BglI BglII Bme1580I BplI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstEII BstYI
Bsu36I BtgI DraIII EarI Eco57MI EcoO109I FalI FspI
HaeII Hin4I HincII KpnI MspA1I NaeI NgoMIV NsiI
NspI PasI PflMI PspOMI PstI PvuII SapI ScaI
SexAI SfcI SmaI SmlI SrfI TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AhdI AleI AloI
ApaLI AscI AseI AsiSI AvrII BbeI BcgI BciVI
BclI BlpI BmgBI BmrI BmtI BpmI BsaAI BsaBI
BsaHI BsiEI BsiWI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspAI HindIII HpaI KasI MfeI MluI MmeI
MscI MslI NarI NcoI NdeI NheI NotI NruI
PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII Tth111I XbaI
XhoI ZraI