(Circular) (Six-base) MAPSORT of: pFN21ASDA1269.seq Check: 5003 from: 1 to: 6319 pFN21ASDA1269 6319 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2552 3133 2552 Size: 581 5738 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2283 2459 2762 3041 3251 4382 5766 6034 Size: 651 176 303 279 210 1131 1384 268 Cuts at: 6034 1632 Size: 1917 Fragments arranged by size: 1917 1384 1131 651 303 279 268 210 176 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 3372 5218 6195 1203 Size: 2169 1846 977 1327 Fragments arranged by size: 2169 1846 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 2087 3521 1166 Size: 921 1434 3964 Fragments arranged by size: 3964 1434 921 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AlwNI CAG_nnn'CTG Cuts at: 2791 3542 4062 5634 2791 Size: 751 520 1572 3476 Fragments arranged by size: 3476 1572 751 520 ApaI G_GGCC'C Cuts at: 3476 3476 Size: 6319 ApaLI G'TGCA_C Cuts at: 2045 2414 4376 5532 2045 Size: 369 1962 1156 2832 Fragments arranged by size: 2832 1962 1156 369 ApoI r'AATT_y Cuts at: 1493 2408 3628 3854 4006 6115 1493 Size: 915 1220 226 152 2109 1697 Fragments arranged by size: 2109 1697 1220 915 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2961 3178 3305 3548 3644 1870 Size: 73 1018 217 127 243 96 4545 Fragments arranged by size: 4545 1018 243 217 127 96 73 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3271 3640 5095 618 Size: 325 217 627 1484 369 1455 1842 Fragments arranged by size: 1842 1484 1455 627 369 325 217 BanII G_rGCy'C Cuts at: 1391 2578 3476 3638 1391 Size: 1187 898 162 4072 Fragments arranged by size: 4072 1187 898 162 BbeI G_GCGC'C Cuts at: 3275 3275 Size: 6319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2123 2688 928 Size: 971 224 565 4559 Fragments arranged by size: 4559 971 565 224 BbvCI CC'TCA_GC Cuts at: 3041 3041 Size: 6319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3305 4211 5421 3305 Size: 906 1210 4203 Fragments arranged by size: 4203 1210 906 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3134 3167 4930 Size: 107 122 71 631 605 1462 33 1763 Cuts at: 4930 136 Size: 1525 Fragments arranged by size: 1763 1525 1462 631 605 122 107 71 33 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 3736 3736 Size: 6319 Bme1580I G_kGCm'C Cuts at: 1790 2049 2418 3189 3213 3476 3534 4380 5536 Size: 259 369 771 24 263 58 846 1156 Cuts at: 5536 1790 Size: 2573 Fragments arranged by size: 2573 1156 846 771 369 263 259 58 24 BmgBI CAC'GTC Cuts at: 1318 2038 2601 2641 1318 Size: 720 563 40 4996 Fragments arranged by size: 4996 720 563 40 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3148 3158 4050 Size: 416 53 83 399 24 1731 10 892 Cuts at: 4050 5008 442 Size: 958 1753 Fragments arranged by size: 1753 1731 958 892 416 399 83 53 24 10 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2345 2345 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2356 2999 3496 4979 1239 Size: 1117 643 497 1483 2579 Fragments arranged by size: 2579 1483 1117 643 497 Bpu10I CC'TnA_GC Cuts at: 2480 2588 3041 3392 2480 Size: 108 453 351 5407 Fragments arranged by size: 5407 453 351 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2219 4444 5309 5607 2219 Size: 2225 865 298 2931 Fragments arranged by size: 2931 2225 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2655 2666 2792 3076 4982 882 Size: 617 1156 11 126 284 1906 2219 Fragments arranged by size: 2219 1906 1156 617 284 126 11 BsaAI yAC'GTr Cuts at: 493 1204 3110 3616 4110 493 Size: 711 1906 506 494 2702 Fragments arranged by size: 2702 1906 711 506 494 BsaBI GATnn'nnATC Cuts at: 2997 6313 2997 Size: 3316 3003 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2748 3065 3272 4126 Size: 53 83 186 936 1215 317 207 854 Cuts at: 4126 4508 5143 275 Size: 382 635 1451 Fragments arranged by size: 1451 1215 936 854 635 382 317 207 186 83 53 BsaWI w'CCGG_w Cuts at: 2077 4752 5150 5424 5571 5982 2077 Size: 2675 398 274 147 411 2414 Fragments arranged by size: 2675 2414 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2565 2595 2972 3002 1178 Size: 30 165 30 1162 30 377 30 4495 Fragments arranged by size: 4495 1162 377 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2215 3572 1186 Size: 883 146 1357 3933 Fragments arranged by size: 3933 1357 883 146 BseYI C'CCAG_C Cuts at: 2229 3033 3195 3347 5522 6018 2229 Size: 804 162 152 2175 496 2530 Fragments arranged by size: 2530 2175 804 496 162 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2897 3428 1163 Size: 573 1161 531 4054 Fragments arranged by size: 4054 1161 573 531 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2607 3129 3402 3769 4530 5558 Size: 275 186 606 522 273 367 761 1028 Cuts at: 5558 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 606 522 367 275 273 186 BsiHKAI G_wGCw'C Cuts at: 2049 2418 3054 3150 3398 3564 4380 4465 5536 Size: 369 636 96 248 166 816 85 1071 Cuts at: 5536 2049 Size: 2832 Fragments arranged by size: 2832 1071 816 636 369 248 166 96 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2418 2578 3054 3150 3189 3213 Size: 399 259 369 160 476 96 39 24 Cuts at: 3213 3398 3476 3534 3564 3638 4380 4465 5536 Size: 185 78 58 30 74 742 85 1071 Cuts at: 5536 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 476 399 369 259 185 160 96 85 78 74 58 39 30 24 BspEI T'CCGG_A Cuts at: 2077 5982 2077 Size: 3905 2414 BspHI T'CATG_A Cuts at: 3054 4208 5938 3054 Size: 1154 1730 3435 Fragments arranged by size: 3435 1730 1154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2552 3133 3674 844 Size: 1708 581 541 3489 Fragments arranged by size: 3489 1708 581 541 BsrBI CCG'CTC Cuts at: 1394 1823 2943 3607 4206 1394 Size: 429 1120 664 599 3507 Fragments arranged by size: 3507 1120 664 599 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4814 4988 65 Size: 1183 3566 174 1396 Fragments arranged by size: 3566 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2750 3067 3124 4963 5150 1700 Size: 110 940 317 57 1839 187 2869 Fragments arranged by size: 2869 1839 940 317 187 110 57 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 3602 4379 5391 3602 Size: 777 1012 4530 Fragments arranged by size: 4530 1012 777 BstAPI GCAn_nnn'nTGC Cuts at: 794 3542 794 Size: 2748 3571 BstBI TT'CG_AA Cuts at: 2007 5123 2007 Size: 3116 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstXI CCAn_nnnn'nTGG Cuts at: 3250 3355 3580 3250 Size: 105 225 5989 Fragments arranged by size: 5989 225 105 BstYI r'GATC_y Cuts at: 1972 3360 3649 4016 4401 4418 5870 5956 5968 Size: 1388 289 367 385 17 1452 86 12 Cuts at: 5968 6314 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1388 385 367 346 289 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3555 3555 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 2022 2208 2700 3132 3588 513 Size: 552 957 186 492 432 456 3244 Fragments arranged by size: 3244 957 552 492 456 432 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5580 Fragments arranged by size: 5580 683 56 BtsI GCAGTG_nn' Cuts at: 2082 2094 2104 2993 3835 4629 4649 2082 Size: 12 10 889 842 794 20 3752 Fragments arranged by size: 3752 889 842 794 20 12 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2421 2604 2703 Size: 54 1425 50 275 18 591 183 99 Cuts at: 2703 2916 3126 3399 3567 3766 4655 6284 8 Size: 213 210 273 168 199 889 1629 43 Fragments arranged by size: 1629 1425 889 591 275 273 213 210 199 183 168 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2604 3126 3399 3766 1537 Size: 275 792 522 273 367 4090 Fragments arranged by size: 4090 792 522 367 275 273 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 2444 3334 4249 1353 Size: 906 185 890 915 3423 Fragments arranged by size: 3423 915 906 890 185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2356 2459 2762 2999 3041 3251 Size: 393 651 73 103 303 237 42 210 Cuts at: 3251 3496 4382 4979 5766 6034 1239 Size: 245 886 597 787 268 1524 Fragments arranged by size: 1524 886 787 651 597 393 303 268 245 237 210 103 73 42 EcoNI CCTnn'n_nnAGG Cuts at: 3254 3254 Size: 6319 EcoO109I rG'GnC_Cy Cuts at: 2830 2965 3473 2830 Size: 135 508 5676 Fragments arranged by size: 5676 508 135 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 3173 3275 5466 1678 Size: 1495 102 2191 2531 Fragments arranged by size: 2531 2191 1495 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3303 3335 4960 4992 5034 Size: 32 135 32 1731 32 1625 32 42 Cuts at: 5034 5066 1373 Size: 32 2626 Fragments arranged by size: 2626 1731 1625 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KasI G'GCGC_C Cuts at: 3271 3271 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3519 3918 3519 Size: 399 5920 MluI A'CGCG_T Cuts at: 3372 6195 3372 Size: 2823 3496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2562 5433 5617 1305 Size: 1257 2871 184 2007 Fragments arranged by size: 2871 2007 1257 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2918 10 Size: 54 1425 343 1086 3411 Fragments arranged by size: 3411 1425 1086 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 2363 2462 3521 3578 4277 4636 Size: 648 921 276 99 1059 57 699 359 Cuts at: 4636 4795 518 Size: 159 2042 Fragments arranged by size: 2042 1059 921 699 648 359 276 159 99 57 MspA1I CmG'CkG Cuts at: 1606 2195 2204 2210 2702 2821 3199 3301 3445 Size: 589 9 6 492 119 378 102 144 Cuts at: 3445 3451 3735 4412 5560 5805 1606 Size: 6 284 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 589 492 378 284 245 144 119 102 9 6 6 NaeI GCC'GGC Cuts at: 2752 2752 Size: 6319 NarI GG'CG_CC Cuts at: 3272 3272 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2022 3132 3588 513 Size: 552 957 1110 456 3244 Fragments arranged by size: 3244 1110 957 552 456 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 2750 2750 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2393 2893 3677 5222 1132 Size: 97 1164 500 784 1545 2229 Fragments arranged by size: 2229 1545 1164 784 500 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 2791 1108 Size: 1683 4636 PfoI T'CCnGG_A Cuts at: 2772 2826 2772 Size: 54 6265 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PmlI CAC'GTG Cuts at: 3110 3110 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2830 2965 2830 Size: 135 6184 PshAI GACnn'nnGTC Cuts at: 1613 3068 1613 Size: 1455 4864 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspOMI G'GGCC_C Cuts at: 3472 3472 Size: 6319 PstI C_TGCA'G Cuts at: 830 2089 2817 2844 3450 3486 3671 830 Size: 1259 728 27 606 36 185 3478 Fragments arranged by size: 3478 1259 728 606 185 36 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 PvuII CAG'CTG Cuts at: 2204 3199 3451 2204 Size: 995 252 5072 Fragments arranged by size: 5072 995 252 SacII CC_GC'GG Cuts at: 2211 2703 2211 Size: 492 5827 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SexAI A'CCwGG_T Cuts at: 2898 2898 Size: 6319 SfcI C'TryA_G Cuts at: 826 1045 2085 2813 2840 3446 3482 3667 4802 Size: 219 1040 728 27 606 36 185 1135 Cuts at: 4802 5483 5674 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1040 728 681 606 219 191 185 36 27 SfiI GGCCn_nnn'nGGCC Cuts at: 3134 3134 Size: 6319 SfoI GGC'GCC Cuts at: 3273 3273 Size: 6319 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 2963 3180 3550 3646 1872 Size: 1091 217 370 96 4545 Fragments arranged by size: 4545 1091 370 217 96 SmlI C'TyrA_G Cuts at: 820 1017 1943 2234 4423 5324 5586 820 Size: 197 926 291 2189 901 262 1553 Fragments arranged by size: 2189 1553 926 901 291 262 197 SnaBI TAC'GTA Cuts at: 493 3616 4110 493 Size: 3123 494 2702 Fragments arranged by size: 3123 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 2893 3677 1132 Size: 1761 784 3774 Fragments arranged by size: 3774 1761 784 SrfI GCCC'GGGC Cuts at: 3180 3180 Size: 6319 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 2811 3500 1517 Size: 1294 689 4336 Fragments arranged by size: 4336 1294 689 StyI C'CwwG_G Cuts at: 513 1065 2022 3012 3132 3214 3588 3758 513 Size: 552 957 990 120 82 374 170 3074 Fragments arranged by size: 3074 990 957 552 374 170 120 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3167 4346 4363 4516 4701 5146 325 Size: 1255 1587 1179 17 153 185 445 1498 Fragments arranged by size: 1587 1498 1255 1179 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2610 2857 4565 Size: 274 80 33 51 1443 633 247 1708 Cuts at: 4565 6277 96 Size: 1712 138 Fragments arranged by size: 1712 1708 1443 633 274 247 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2565 2597 2972 3004 6279 6311 2565 Size: 32 375 32 3275 32 2573 Fragments arranged by size: 3275 2573 375 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2528 2765 1316 Size: 1212 237 4870 Fragments arranged by size: 4870 1212 237 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3503 3577 3503 Size: 74 6245 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 2961 3178 3548 3644 1870 Size: 1091 217 370 96 4545 Fragments arranged by size: 4545 1091 370 217 96 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SanDI SapI SwaI