Restriction map for pFN21ASDA1267
##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       AccIII      AclI        AgeI        AhdI        
Alw44I      ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AspI        BalI        
BbrPI       BcgI        BglII       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     ClaI        
CpoI        DraI        DrdI        Eam1105I    EcoNI       
EcoO65I     EcoRV       EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        KspI        MluI        
MluNI       MroI        MscI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I       1452  GGTACC               G^GTACC
AccI         1602  GTCTAC               GT^[AC][GT]AC
AccII        2626  CGCG                 CG^CG
AcyI           12  GGCGCC               G[GA]^CG[CT]C
AflII        2113  CTTAAG               C^TTAAG
AflIII       1777  ACACGT               A^C[GA][CT]GT
AocI         3200  CCTCAGG              CC^T[ACGT]AGG
Asp718I      1452  GGTACC               G^GTACC
AviII        1897  TGCGCA               TGC^GCA
AvrII        2965  CCTAGG               C^CTAGG
BamHI         171  GGATCC               G^GATCC
BbeI           12  GGCGCC               GGCGC^C
BbvCI        1699  GCTGAGG              CCTCAGC(-5/-2)
BciVI        3048  GGATAC               GTATCC(6/5)
BclI          504  TGATCA               T^GATCA
BfrI         2113  CTTAAG               C^TTAAG
BglI         2661  GCCCAGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2965  CCTAGG               C^CTAGG
BpmI          759  CTCCAG               CTGGAG(16/14)
BsaAI        1418  TACGTG               [CT]AC^GT[GA]
BsaHI          12  GGCGCC               G[GA]^CG[CT]C
BsiEI        2856  CGGCCG               CG[GA][CT]^CG
BspHI         507  TCATGA               T^CATGA
BsrDI         159  CATTGC               GCAATG(2/0)
BsrFI         717  GCCGGC               [GA]^CCGG[CT]
BssSI        2805  CACGAG               CACGAG(-5/-1)
BstUI        2626  CGCG                 CG^CG
Bsu36I       3200  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        717  GCCGGC               [GA]^CCGG[CT]
DraIII       1901  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         2856  CGGCCG               C^GGCCG
EclXI        2856  CGGCCG               C^GGCCG
Eco52I       2856  CGGCCG               C^GGCCG
Eco81I       3200  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1385  GAATTC               G^AATTC
FbaI          504  TGATCA               T^GATCA
FspI         1897  TGCGCA               TGC^GCA
Hin1I          12  GGCGCC               G[GA]^CG[CT]C
KasI           12  GGCGCC               G^GCGCC
KpnI         1452  GGTACC               GGTAC^C
MfeI          618  CAATTG               C^AATTG
MunI          618  CAATTG               C^AATTG
MvnI         2626  CGCG                 CG^CG
NaeI          717  GCCGGC               GCC^GGC
NarI           12  GGCGCC               GG^CGCC
NgoMIV        717  GCCGGC               G^CCGGC
PshAI        1292  GACGCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          507  TCATGA               T^CATGA
SacI         2571  GAGCTC               GAGCT^C
SapI          559  GCTCTTC              GCTCTTC(1/4)
SexAI         747  ACCTGGT              A^CC[AT]GGT
SfoI           12  GGCGCC               GGC^GCC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI         1385  GAATTC               [GA]^AATT[CT]
             1940  AAATTC              
Aor51HI       908  AGCGCT               AGC^GCT
             2306  AGCGCT              
ApoI         1385  GAATTC               [GA]^AATT[CT]
             1940  AAATTC              
AvaI         1267  CCCGAG               C^[CT]CG[GA]G
             3017  CTCGGG              
BlpI          364  GCTGAGC              GC^T[ACGT]AGC
             3295  GCTCAGC             
Bpu1102I      364  GCTGAGC              GC^T[ACGT]AGC
             3295  GCTCAGC             
BsaBI         584  GATCTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1882  GATGTGAATC          
BsaWI          53  TCCGGT               [AT]^CCGG[AT]
             1853  ACCGGA              
BsmBI        1737  CGTCTC               CGTCTC(1/5)
             1290  GAGACG              
BsoBI        1267  CCCGAG               C^[CT]CG[GA]G
             3017  CTCGGG              
BsrBI          17  CCGCTC               CCGCTC(-3/-3)
             3106  GAGCGG              
BstYI         171  GGATCC               [GA]^GATC[CT]
              583  GGATCT              
CelII         364  GCTGAGC              GC^T[ACGT]AGC
             3295  GCTCAGC             
EaeI         2856  CGGCCG               [CT]^GGCC[GA]
             3216  TGGCCG              
Eco47III      908  AGCGCT               AGC^GCT
             2306  AGCGCT              
MamI          584  GATCTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1882  GATGTGAATC          
MflI          171  GGATCC               [GA]^GATC[CT]
              583  GGATCT              
NspI         2048  GCATGC               [GA]CATG^[CT]
             2760  GCATGC              
PstI          164  CTGCAG               CTGCA^G
             1316  CTGCAG              
SphI         2048  GCATGC               GCATG^C
             2760  GCATGC              
XhoII         171  GGATCC               [GA]^GATC[CT]
              583  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpuAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        
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