(Circular) (Six-base) MAPSORT of: pFN21ASDA1266.seq Check: 5598 from: 1 to: 6340 pFN21ASDA1266 6340 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3683 1937 Size: 1746 4594 Acc65I G'GTAC_C Cuts at: 3661 3661 Size: 6340 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2697 4403 5787 6055 1632 Size: 1065 1706 1384 268 1917 Fragments arranged by size: 1917 1706 1384 1065 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 2787 3174 5239 6216 1203 Size: 1584 387 2065 977 1327 Fragments arranged by size: 2065 1584 1327 977 387 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 5069 5069 Size: 6340 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6340 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2541 1184 Size: 1357 4983 AlwNI CAG_nnn'CTG Cuts at: 2675 2843 4083 5655 2675 Size: 168 1240 1572 3360 Fragments arranged by size: 3360 1572 1240 168 ApaI G_GGCC'C Cuts at: 3035 3035 Size: 6340 ApaLI G'TGCA_C Cuts at: 4397 5553 4397 Size: 1156 5184 ApoI r'AATT_y Cuts at: 1493 3281 3649 3875 4027 6136 1493 Size: 1788 368 226 152 2109 1697 Fragments arranged by size: 2109 1788 1697 368 226 152 AseI AT'TA_AT Cuts at: 160 1032 3351 4894 160 Size: 872 2319 1543 1606 Fragments arranged by size: 2319 1606 1543 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 2541 3665 1870 Size: 73 598 1124 4545 Fragments arranged by size: 4545 1124 598 73 BamHI G'GATC_C Cuts at: 2401 3670 4037 2401 Size: 1269 367 4704 Fragments arranged by size: 4704 1269 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3661 5116 618 Size: 325 217 627 1874 1455 1842 Fragments arranged by size: 1874 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2482 3035 3659 1391 Size: 1091 553 624 4072 Fragments arranged by size: 4072 1091 624 553 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3245 928 Size: 971 1346 4023 Fragments arranged by size: 4023 1346 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4531 4565 1903 Size: 34 2594 34 3678 Fragments arranged by size: 3678 2594 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2230 2563 4232 5442 2230 Size: 333 1669 1210 3128 Fragments arranged by size: 3128 1669 1210 333 BclI T'GATC_A Cuts at: 1548 1548 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3368 4951 136 Size: 107 122 71 631 605 1696 1583 1525 Fragments arranged by size: 1696 1583 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6335 6335 Size: 6340 BlpI GC'TnA_GC Cuts at: 3757 3757 Size: 6340 Bme1580I G_kGCm'C Cuts at: 1790 3035 4401 5557 1790 Size: 1245 1366 1156 2573 Fragments arranged by size: 2573 1366 1245 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6340 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3106 3225 4071 Size: 416 53 83 399 24 1689 119 846 Cuts at: 4071 5029 442 Size: 958 1753 Fragments arranged by size: 1753 1689 958 846 416 399 119 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2685 1056 Size: 1629 4711 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5000 1239 Size: 3761 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4465 5330 5628 4465 Size: 865 298 5177 Fragments arranged by size: 5177 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5003 882 Size: 617 3504 2219 Fragments arranged by size: 3504 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2456 3175 3618 3637 4131 493 Size: 711 1252 719 443 19 494 2702 Fragments arranged by size: 2702 1252 719 711 494 443 19 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4147 4529 5164 275 Size: 53 83 186 936 2614 382 635 1451 Fragments arranged by size: 2614 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4773 5171 5445 5592 6003 4773 Size: 398 274 147 411 5110 Fragments arranged by size: 5110 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2622 2651 2652 2681 2971 Size: 30 165 30 1219 29 1 29 290 Cuts at: 2971 3001 1178 Size: 30 4517 Fragments arranged by size: 4517 1219 290 165 30 30 30 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6340 BseYI C'CCAG_C Cuts at: 5543 6039 5543 Size: 496 5844 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2200 1163 Size: 573 464 5303 Fragments arranged by size: 5303 573 464 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3338 3790 4551 5579 1540 Size: 275 186 1337 452 761 1028 2301 Fragments arranged by size: 2301 1337 1028 761 452 275 186 BsiHKAI G_wGCw'C Cuts at: 4401 4486 5557 4401 Size: 85 1071 5184 Fragments arranged by size: 5184 1071 85 BsmI GAATG_Cn' Cuts at: 3204 3567 3851 3944 3204 Size: 363 284 93 5600 Fragments arranged by size: 5600 363 284 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2482 3035 3659 4401 4486 5557 1391 Size: 399 692 553 624 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 692 624 553 399 85 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 4229 5959 4229 Size: 1730 4610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3695 844 Size: 2851 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4227 1394 Size: 429 2404 3507 Fragments arranged by size: 3507 2404 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4835 5009 65 Size: 1183 3587 174 1396 Fragments arranged by size: 3587 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4984 5171 1700 Size: 110 3174 187 2869 Fragments arranged by size: 3174 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 3159 4400 5412 3159 Size: 1241 1012 4087 Fragments arranged by size: 4087 1241 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3199 3377 794 Size: 2405 178 3757 Fragments arranged by size: 3757 2405 178 BstBI TT'CG_AA Cuts at: 2007 5144 2007 Size: 3137 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6340 BstXI CCAn_nnnn'nTGG Cuts at: 2482 2482 Size: 6340 BstYI r'GATC_y Cuts at: 1972 2401 3465 3670 4037 4422 4439 5891 5977 Size: 429 1064 205 367 385 17 1452 86 Cuts at: 5977 5989 6335 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1064 429 385 367 346 205 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5788 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5601 Fragments arranged by size: 5601 683 56 BtsI GCAGTG_nn' Cuts at: 2188 2608 3053 3240 3856 4650 4670 2188 Size: 420 445 187 616 794 20 3858 Fragments arranged by size: 3858 794 616 445 420 187 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6305 DraI TTT'AAA Cuts at: 3644 3644 Size: 6340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5347 809 Size: 507 4031 1802 Fragments arranged by size: 4031 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3335 3787 4676 Size: 54 1425 50 275 18 1505 452 889 Cuts at: 4676 6305 8 Size: 1629 43 Fragments arranged by size: 1629 1505 1425 889 452 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3335 3787 1537 Size: 275 1523 452 4090 Fragments arranged by size: 4090 1523 452 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 3117 4270 1353 Size: 1335 429 1153 3423 Fragments arranged by size: 3423 1335 1153 429 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4927 5301 5447 387 Size: 1064 138 3338 374 146 1280 Fragments arranged by size: 3338 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2697 4403 5000 5787 6055 1239 Size: 393 1065 1706 597 787 268 1524 Fragments arranged by size: 1706 1524 1065 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2973 2973 Size: 6340 EcoO109I rG'GnC_Cy Cuts at: 2312 2633 3031 3154 2312 Size: 321 398 123 5498 Fragments arranged by size: 5498 398 321 123 EcoRI G'AATT_C Cuts at: 3649 4027 3649 Size: 378 5962 EcoRV GAT'ATC Cuts at: 2321 2321 Size: 6340 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2762 2762 Size: 6340 FspI TGC'GCA Cuts at: 4846 4846 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 5487 1678 Size: 3809 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 2192 2224 2651 Size: 32 135 32 473 32 115 32 427 Cuts at: 2651 2683 3116 3148 4981 5013 5055 5087 1373 Size: 32 433 32 1833 32 42 32 2626 Fragments arranged by size: 2626 1833 473 433 427 135 115 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3684 3930 669 Size: 988 281 1746 246 3079 Fragments arranged by size: 3079 1746 988 281 246 HindIII A'AGCT_T Cuts at: 748 3249 748 Size: 2501 3839 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KpnI G_GTAC'C Cuts at: 3665 3665 Size: 6340 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5454 5638 1305 Size: 4149 184 2007 Fragments arranged by size: 4149 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4518 Fragments arranged by size: 4518 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2523 2589 3597 4298 4657 4816 518 Size: 648 1357 66 1008 701 359 159 2042 Fragments arranged by size: 2042 1357 1008 701 648 359 159 66 MspA1I CmG'CkG Cuts at: 1606 3756 4433 5581 5826 1606 Size: 2150 677 1148 245 2120 Fragments arranged by size: 2150 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5788 NdeI CA'TA_TG Cuts at: 387 2520 387 Size: 2133 4207 NheI G'CTAG_C Cuts at: 1052 2681 1052 Size: 1629 4711 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 2042 2791 3392 3447 3698 5243 1132 Size: 97 813 749 601 55 251 1545 2229 Fragments arranged by size: 2229 1545 813 749 601 251 97 55 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6340 PciI A'CATG_T Cuts at: 2787 5239 2787 Size: 2452 3888 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6340 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PmlI CAC'GTG Cuts at: 2456 3175 3618 2456 Size: 719 443 5178 Fragments arranged by size: 5178 719 443 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4409 4441 5922 5954 4409 Size: 32 1481 32 4795 Fragments arranged by size: 4795 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2312 2633 3154 2312 Size: 321 521 5498 Fragments arranged by size: 5498 521 321 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6340 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PspOMI G'GGCC_C Cuts at: 3031 3031 Size: 6340 PstI C_TGCA'G Cuts at: 830 2536 3235 3692 830 Size: 1706 699 457 3478 Fragments arranged by size: 3478 1706 699 457 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SbfI CC_TGCA'GG Cuts at: 3692 3692 Size: 6340 ScaI AGT'ACT Cuts at: 4588 6300 4588 Size: 1712 4628 SfcI C'TryA_G Cuts at: 826 1045 2532 3147 3231 3586 3688 4823 5504 Size: 219 1487 615 84 355 102 1135 681 Cuts at: 5504 5695 826 Size: 191 1471 Fragments arranged by size: 1487 1471 1135 681 615 355 219 191 102 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 3667 1872 Size: 1795 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4444 5345 5607 820 Size: 197 926 2501 901 262 1553 Fragments arranged by size: 2501 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3637 4131 493 Size: 3144 494 2702 Fragments arranged by size: 3144 2702 494 SpeI A'CTAG_T Cuts at: 152 2906 152 Size: 2754 3586 SphI G_CATG'C Cuts at: 1132 2042 3698 1132 Size: 910 1656 3774 Fragments arranged by size: 3774 1656 910 SspI AAT'ATT Cuts at: 5 52 2780 4264 5 Size: 47 2728 1484 2081 Fragments arranged by size: 2728 2081 1484 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6340 StyI C'CwwG_G Cuts at: 513 1065 2750 3779 513 Size: 552 1685 1029 3074 Fragments arranged by size: 3074 1685 1029 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 3125 4367 4384 4537 4722 5167 Size: 1255 525 1020 1242 17 153 185 445 Cuts at: 5167 325 Size: 1498 Fragments arranged by size: 1498 1255 1242 1020 525 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4586 6298 96 Size: 274 80 33 51 1443 2609 1712 138 Fragments arranged by size: 2609 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2908 2940 2971 3003 3205 3237 6300 6332 2908 Size: 32 31 32 202 32 3063 32 2916 Fragments arranged by size: 3063 2916 202 32 32 32 32 31 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6340 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6340 XmaI C'CCGG_G Cuts at: 1870 3665 1870 Size: 1795 4545 XmnI GAAnn'nnTTC Cuts at: 4469 4469 Size: 6340 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI