(Circular) (Six-base) MAPSORT of: pFN21ASDA1264.seq Check: 5119 from: 1 to: 6886 pFN21ASDA1264 6886 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4696 278 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4229 1937 Size: 2292 4594 Acc65I G'GTAC_C Cuts at: 3150 4207 3150 Size: 1057 5829 AclI AA'CG_TT Cuts at: 1563 5013 5386 1563 Size: 3450 373 3063 Fragments arranged by size: 3450 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4017 4949 6333 6601 1632 Size: 2385 932 1384 268 1917 Fragments arranged by size: 2385 1917 1384 932 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6689 AflIII A'CryG_T Cuts at: 1203 3546 5785 6762 1203 Size: 2343 2239 977 1327 Fragments arranged by size: 2343 2239 1327 977 AgeI A'CCGG_T Cuts at: 5717 5717 Size: 6886 AhdI GACnn_n'nnGTC Cuts at: 3901 5615 3901 Size: 1714 5172 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6886 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6886 AlwNI CAG_nnn'CTG Cuts at: 3062 4629 6201 3062 Size: 1567 1572 3747 Fragments arranged by size: 3747 1572 1567 ApaI G_GGCC'C Cuts at: 3083 3083 Size: 6886 ApaLI G'TGCA_C Cuts at: 4943 6099 4943 Size: 1156 5730 ApoI r'AATT_y Cuts at: 1493 4195 4421 4573 6682 1493 Size: 2702 226 152 2109 1697 Fragments arranged by size: 2702 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5440 160 Size: 872 4408 1606 Fragments arranged by size: 4408 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 AvaI C'yCGr_G Cuts at: 1870 1943 2689 2890 3908 4211 1870 Size: 73 746 201 1018 303 4545 Fragments arranged by size: 4545 1018 746 303 201 73 BamHI G'GATC_C Cuts at: 2491 3984 4216 4583 2491 Size: 1493 232 367 4794 Fragments arranged by size: 4794 1493 367 232 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3150 3608 3811 4207 5662 Size: 325 217 627 1363 458 203 396 1455 Cuts at: 5662 618 Size: 1842 Fragments arranged by size: 1842 1455 1363 627 458 396 325 217 203 BanII G_rGCy'C Cuts at: 1391 2994 3083 3230 3275 3579 3688 3945 4077 Size: 1603 89 147 45 304 109 257 132 Cuts at: 4077 4205 1391 Size: 128 4072 Fragments arranged by size: 4072 1603 304 257 147 132 128 109 89 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2231 2823 928 Size: 971 332 592 4991 Fragments arranged by size: 4991 971 592 332 BbvCI CC'TCA_GC Cuts at: 2856 3041 3233 2856 Size: 185 192 6509 Fragments arranged by size: 6509 192 185 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2417 2451 5077 5111 1903 Size: 34 480 34 2626 34 3678 Fragments arranged by size: 3678 2626 480 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4778 5988 4778 Size: 1210 5676 BclI T'GATC_A Cuts at: 1548 3510 1548 Size: 1962 4924 BfrBI ATG'CAT Cuts at: 2196 3414 2196 Size: 1218 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5497 136 Size: 107 122 71 631 605 3825 1525 Fragments arranged by size: 3825 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6881 6881 Size: 6886 BlpI GC'TnA_GC Cuts at: 4303 4303 Size: 6886 Bme1580I G_kGCm'C Cuts at: 1790 2535 3083 4947 6103 1790 Size: 745 548 1864 1156 2573 Fragments arranged by size: 2573 1864 1156 745 548 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6886 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2100 2404 4617 Size: 416 53 83 399 24 683 304 2213 Cuts at: 4617 5575 442 Size: 958 1753 Fragments arranged by size: 2213 1753 958 683 416 399 304 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6886 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2344 2882 3280 3953 5546 1239 Size: 1105 538 398 673 1593 2579 Fragments arranged by size: 2579 1593 1105 673 538 398 Bpu10I CC'TnA_GC Cuts at: 2856 3041 3224 3233 2856 Size: 185 183 9 6509 Fragments arranged by size: 6509 185 183 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 3109 3780 5011 5876 6174 2651 Size: 458 671 1231 865 298 3363 Fragments arranged by size: 3363 1231 865 671 458 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3345 5549 882 Size: 617 1846 2204 2219 Fragments arranged by size: 2219 2204 1846 617 BsaAI yAC'GTr Cuts at: 493 1204 2536 4183 4677 493 Size: 711 1332 1647 494 2702 Fragments arranged by size: 2702 1647 1332 711 494 BsaBI GATnn'nnATC Cuts at: 2490 3021 6880 2490 Size: 531 3859 2496 Fragments arranged by size: 3859 2496 531 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4693 5075 5710 275 Size: 53 83 186 936 3160 382 635 1451 Fragments arranged by size: 3160 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3468 5319 5717 5991 6138 6549 3468 Size: 1851 398 274 147 411 3805 Fragments arranged by size: 3805 1851 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3212 3242 1178 Size: 30 165 30 1809 30 4822 Fragments arranged by size: 4822 1809 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3265 3268 4094 1186 Size: 2079 3 826 3978 Fragments arranged by size: 3978 2079 826 3 BseYI C'CCAG_C Cuts at: 2130 2798 3263 3267 6089 6585 2130 Size: 668 465 4 2822 496 2431 Fragments arranged by size: 2822 2431 668 496 465 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6313 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2451 3025 4336 5097 6125 1540 Size: 275 186 450 574 1311 761 1028 2301 Fragments arranged by size: 2301 1311 1028 761 574 450 275 186 BsiHKAI G_wGCw'C Cuts at: 2759 3031 3230 3579 3688 3796 4041 4947 5032 Size: 272 199 349 109 108 245 906 85 Cuts at: 5032 6103 2759 Size: 1071 3542 Fragments arranged by size: 3542 1071 906 349 272 245 199 109 108 85 BsmI GAATG_Cn' Cuts at: 3003 4397 4490 3003 Size: 1394 93 5399 Fragments arranged by size: 5399 1394 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3475 3475 Size: 6886 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2535 2759 2994 3031 3083 3230 3275 Size: 399 745 224 235 37 52 147 45 Cuts at: 3275 3579 3688 3796 3945 4041 4077 4205 4947 Size: 304 109 108 149 96 36 128 742 Cuts at: 4947 5032 6103 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 745 742 399 304 235 224 149 147 128 109 108 96 85 52 45 37 36 BspEI T'CCGG_A Cuts at: 3468 6549 3468 Size: 3081 3805 BspHI T'CATG_A Cuts at: 4158 4775 6505 4158 Size: 617 1730 4539 Fragments arranged by size: 4539 1730 617 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4121 4241 844 Size: 3277 120 3489 Fragments arranged by size: 3489 3277 120 BsrBI CCG'CTC Cuts at: 1394 1823 4773 1394 Size: 429 2950 3507 Fragments arranged by size: 3507 2950 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5381 5555 65 Size: 1183 4133 174 1396 Fragments arranged by size: 4133 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2120 3401 5530 5717 1700 Size: 110 310 1281 2129 187 2869 Fragments arranged by size: 2869 2129 1281 310 187 110 BsrGI T'GTAC_A Cuts at: 96 3549 96 Size: 3453 3433 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6886 BssSI C'ACGA_G Cuts at: 4946 5958 4946 Size: 1012 5874 BstAPI GCAn_nnn'nTGC Cuts at: 794 3411 794 Size: 2617 4269 BstBI TT'CG_AA Cuts at: 2007 5690 2007 Size: 3683 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6886 BstXI CCAn_nnnn'nTGG Cuts at: 2212 2212 Size: 6886 BstYI r'GATC_y Cuts at: 1972 2491 3984 4216 4583 4968 4985 6437 6523 Size: 519 1493 232 367 385 17 1452 86 Cuts at: 6523 6535 6881 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1493 1452 519 385 367 346 232 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2321 3858 2321 Size: 1537 5349 BtgI C'CryG_G Cuts at: 513 1065 2365 3210 513 Size: 552 1300 845 4189 Fragments arranged by size: 4189 1300 845 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3400 3585 3771 3989 487 Size: 683 56 2174 185 186 218 3384 Fragments arranged by size: 3384 2174 683 218 186 185 56 BtsI GCAGTG_nn' Cuts at: 2141 3456 4402 5196 5216 2141 Size: 1315 946 794 20 3811 Fragments arranged by size: 3811 1315 946 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6886 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6851 DraI TTT'AAA Cuts at: 2935 4190 2935 Size: 1255 5631 DraIII CAC_nnn'GTG Cuts at: 3000 3000 Size: 6886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5893 809 Size: 507 4577 1802 Fragments arranged by size: 4577 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 3369 3760 Size: 54 1425 50 275 18 330 1209 391 Cuts at: 3760 4333 5222 6851 8 Size: 573 889 1629 43 Fragments arranged by size: 1629 1425 1209 889 573 391 330 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4333 1537 Size: 275 2521 4090 Fragments arranged by size: 4090 2521 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2462 3812 4816 1353 Size: 1109 1350 1004 3423 Fragments arranged by size: 3423 1350 1109 1004 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5473 5847 5993 387 Size: 1064 138 3884 374 146 1280 Fragments arranged by size: 3884 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3228 3577 3686 3228 Size: 349 109 6428 Fragments arranged by size: 6428 349 109 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2344 2882 3280 3953 4017 4949 5546 Size: 393 712 538 398 673 64 932 597 Cuts at: 5546 6333 6601 1239 Size: 787 268 1524 Fragments arranged by size: 1524 932 787 712 673 597 538 398 393 268 64 EcoNI CCTnn'n_nnAGG Cuts at: 2279 3073 4022 2279 Size: 794 949 5143 Fragments arranged by size: 5143 949 794 EcoO109I rG'GnC_Cy Cuts at: 2795 3079 3080 4015 2795 Size: 284 1 935 5666 Fragments arranged by size: 5666 935 284 1 EcoRI G'AATT_C Cuts at: 4195 4573 4195 Size: 378 6508 FspI TGC'GCA Cuts at: 5392 5392 Size: 6886 HaeII r_GCGC'y Cuts at: 1678 6033 1678 Size: 4355 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2282 2314 2606 2638 2839 Size: 32 135 32 710 32 292 32 201 Cuts at: 2839 2871 3887 3919 5527 5559 5601 5633 1373 Size: 32 1016 32 1608 32 42 32 2626 Fragments arranged by size: 2626 1608 1016 710 292 201 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3544 4230 4476 669 Size: 988 281 1606 686 246 3079 Fragments arranged by size: 3079 1606 988 686 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6886 HpaI GTT'AAC Cuts at: 4476 4476 Size: 6886 KpnI G_GTAC'C Cuts at: 3154 4211 3154 Size: 1057 5829 MfeI C'AATT_G Cuts at: 4485 4485 Size: 6886 MluI A'CGCG_T Cuts at: 6762 6762 Size: 6886 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 2337 3306 6000 6184 1305 Size: 786 246 969 2694 184 2007 Fragments arranged by size: 2694 2007 969 786 246 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3371 10 Size: 54 1425 343 1539 3525 Fragments arranged by size: 3525 1539 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 3534 3638 4844 5203 5362 518 Size: 648 1134 1234 104 1206 359 159 2042 Fragments arranged by size: 2042 1234 1206 1134 648 359 159 104 MspA1I CmG'CkG Cuts at: 1606 2212 2751 3267 3334 4302 4979 6127 6372 Size: 606 539 516 67 968 677 1148 245 Cuts at: 6372 1606 Size: 2120 Fragments arranged by size: 2120 1148 968 677 606 539 516 245 67 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6334 NdeI CA'TA_TG Cuts at: 387 387 Size: 6886 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6886 NotI GC'GGCC_GC Cuts at: 4333 4333 Size: 6886 NsiI A_TGCA'T Cuts at: 2198 3416 2198 Size: 1218 5668 NspI r_CATG'y Cuts at: 1132 1229 3550 4244 5789 1132 Size: 97 2321 694 1545 2229 Fragments arranged by size: 2321 2229 1545 694 97 PasI CC'CwG_GG Cuts at: 1348 3075 3357 1348 Size: 1727 282 4877 Fragments arranged by size: 4877 1727 282 PciI A'CATG_T Cuts at: 3546 5785 3546 Size: 2239 4647 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6886 PfoI T'CCnGG_A Cuts at: 2898 3987 2898 Size: 1089 5797 PmeI GTTT'AAAC Cuts at: 4190 4190 Size: 6886 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4955 4987 6468 6500 4955 Size: 32 1481 32 5341 Fragments arranged by size: 5341 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2795 4015 2795 Size: 1220 5666 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6886 PsiI TTA'TAA Cuts at: 4456 4456 Size: 6886 PspOMI G'GGCC_C Cuts at: 3079 3079 Size: 6886 PspXI vC'TCGA_Gb Cuts at: 2689 2689 Size: 6886 PstI C_TGCA'G Cuts at: 830 2743 3189 3333 4238 830 Size: 1913 446 144 905 3478 Fragments arranged by size: 3478 1913 905 446 144 PvuI CG_AT'CG Cuts at: 2001 3025 2001 Size: 1024 5862 PvuII CAG'CTG Cuts at: 2212 3267 3334 2212 Size: 1055 67 5764 Fragments arranged by size: 5764 1055 67 SacI G_AGCT'C Cuts at: 3230 3579 3688 3230 Size: 349 109 6428 Fragments arranged by size: 6428 349 109 SalI G'TCGA_C Cuts at: 1936 4228 1936 Size: 2292 4594 SanDI GG'GwC_CC Cuts at: 2795 2795 Size: 6886 SbfI CC_TGCA'GG Cuts at: 4238 4238 Size: 6886 ScaI AGT'ACT Cuts at: 5134 6846 5134 Size: 1712 5174 SfcI C'TryA_G Cuts at: 826 1045 2739 3185 3329 4234 5369 6050 6241 Size: 219 1694 446 144 905 1135 681 191 Cuts at: 6241 826 Size: 1471 Fragments arranged by size: 1694 1471 1135 905 681 446 219 191 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6886 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6886 SmaI CCC'GGG Cuts at: 1872 4213 1872 Size: 2341 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 2689 3124 3795 4990 5891 Size: 197 926 723 23 435 671 1195 901 Cuts at: 5891 6153 820 Size: 262 1553 Fragments arranged by size: 1553 1195 926 901 723 671 435 262 197 23 SnaBI TAC'GTA Cuts at: 493 4183 4677 493 Size: 3690 494 2702 Fragments arranged by size: 3690 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6886 SphI G_CATG'C Cuts at: 1132 4244 1132 Size: 3112 3774 SspI AAT'ATT Cuts at: 5 52 4810 5 Size: 47 4758 2081 Fragments arranged by size: 4758 2081 47 StuI AGG'CCT Cuts at: 1517 2561 1517 Size: 1044 5842 StyI C'CwwG_G Cuts at: 513 1065 2760 2947 3706 4325 513 Size: 552 1695 187 759 619 3074 Fragments arranged by size: 3074 1695 759 619 552 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2218 2353 2465 3571 4913 4930 5083 Size: 1255 638 135 112 1106 1342 17 153 Cuts at: 5083 5268 5713 325 Size: 185 445 1498 Fragments arranged by size: 1498 1342 1255 1106 638 445 185 153 135 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2457 3201 3549 Size: 274 80 33 51 1443 480 744 348 Cuts at: 3549 5132 6844 96 Size: 1583 1712 138 Fragments arranged by size: 1712 1583 1443 744 480 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3021 3053 6846 6878 3021 Size: 32 3793 32 3029 Fragments arranged by size: 3793 3029 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6886 XbaI T'CTAG_A Cuts at: 2732 3598 4222 2732 Size: 866 624 5396 Fragments arranged by size: 5396 866 624 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2767 3143 3364 2767 Size: 376 221 6289 Fragments arranged by size: 6289 376 221 XhoI C'TCGA_G Cuts at: 1943 2689 1943 Size: 746 6140 XmaI C'CCGG_G Cuts at: 1870 4211 1870 Size: 2341 4545 XmnI GAAnn'nnTTC Cuts at: 3315 5015 3315 Size: 1700 5186 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4694 276 Size: 53 83 186 936 3160 2468 Fragments arranged by size: 3160 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI