(Circular) (Six-base) MAPSORT of: pFN21ASDA1252.seq Check: 6042 from: 1 to: 6433 pFN21ASDA1252 6433 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4243 278 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2085 2964 3776 1937 Size: 148 879 812 4594 Fragments arranged by size: 4594 879 812 148 Acc65I G'GTAC_C Cuts at: 3754 3754 Size: 6433 AclI AA'CG_TT Cuts at: 1563 4560 4933 1563 Size: 2997 373 3063 Fragments arranged by size: 3063 2997 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2940 4496 5880 6148 1632 Size: 1308 1556 1384 268 1917 Fragments arranged by size: 1917 1556 1384 1308 268 AflII C'TTAA_G Cuts at: 820 1017 2411 820 Size: 197 1394 4842 Fragments arranged by size: 4842 1394 197 AflIII A'CryG_T Cuts at: 1203 2328 5332 6309 1203 Size: 1125 3004 977 1327 Fragments arranged by size: 3004 1327 1125 977 AgeI A'CCGG_T Cuts at: 5264 5264 Size: 6433 AhdI GACnn_n'nnGTC Cuts at: 5162 5162 Size: 6433 AleI CACnn'nnGTG Cuts at: 1166 3255 1166 Size: 2089 4344 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2166 1184 Size: 982 5451 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2584 2616 2584 Size: 32 6401 AlwNI CAG_nnn'CTG Cuts at: 2383 4176 5748 2383 Size: 1793 1572 3068 Fragments arranged by size: 3068 1793 1572 ApaLI G'TGCA_C Cuts at: 4490 5646 4490 Size: 1156 5277 ApoI r'AATT_y Cuts at: 1493 3742 3968 4120 6229 1493 Size: 2249 226 152 2109 1697 Fragments arranged by size: 2249 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4987 160 Size: 872 3955 1606 Fragments arranged by size: 3955 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 AvaI C'yCGr_G Cuts at: 1870 1943 3758 1870 Size: 73 1815 4545 Fragments arranged by size: 4545 1815 73 BamHI G'GATC_C Cuts at: 3383 3763 4130 3383 Size: 380 367 5686 Fragments arranged by size: 5686 380 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3590 3754 5209 618 Size: 325 217 627 1803 164 1455 1842 Fragments arranged by size: 1842 1803 1455 627 325 217 164 BanII G_rGCy'C Cuts at: 1391 2064 2139 2390 2499 3752 1391 Size: 673 75 251 109 1253 4072 Fragments arranged by size: 4072 1253 673 251 109 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2194 3581 3646 928 Size: 971 295 1387 65 3715 Fragments arranged by size: 3715 1387 971 295 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4624 4658 1903 Size: 34 2687 34 3678 Fragments arranged by size: 3678 2687 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3005 4325 5535 3005 Size: 1320 1210 3903 Fragments arranged by size: 3903 1320 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6433 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2933 3236 5044 Size: 107 122 71 631 605 1261 303 1808 Cuts at: 5044 136 Size: 1525 Fragments arranged by size: 1808 1525 1261 631 605 303 122 107 71 BglII A'GATC_T Cuts at: 6428 6428 Size: 6433 BlpI GC'TnA_GC Cuts at: 3850 3850 Size: 6433 Bme1580I G_kGCm'C Cuts at: 1790 2746 4494 5650 1790 Size: 956 1748 1156 2573 Fragments arranged by size: 2573 1748 1156 956 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6433 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3459 4164 5122 Size: 416 53 83 399 24 2042 705 958 Cuts at: 5122 442 Size: 1753 Fragments arranged by size: 2042 1753 958 705 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6433 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2440 3002 2440 Size: 562 5871 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2703 2836 3041 5093 1239 Size: 1464 133 205 2052 2579 Fragments arranged by size: 2579 2052 1464 205 133 Bpu10I CC'TnA_GC Cuts at: 3429 3429 Size: 6433 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3296 4558 5423 5721 3296 Size: 1262 865 298 4008 Fragments arranged by size: 4008 1262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5096 882 Size: 617 3597 2219 Fragments arranged by size: 3597 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3730 4224 493 Size: 711 2526 494 2702 Fragments arranged by size: 2702 2526 711 494 BsaBI GATnn'nnATC Cuts at: 6427 6427 Size: 6433 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2433 4240 4622 5257 Size: 53 83 186 936 900 1807 382 635 Cuts at: 5257 275 Size: 1451 Fragments arranged by size: 1807 1451 936 900 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4866 5264 5538 5685 6096 4866 Size: 398 274 147 411 5203 Fragments arranged by size: 5203 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6208 Fragments arranged by size: 6208 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 1186 Size: 1309 5124 BseYI C'CCAG_C Cuts at: 5636 6132 5636 Size: 496 5937 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5860 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3883 4644 5672 1540 Size: 275 186 1882 761 1028 2301 Fragments arranged by size: 2301 1882 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2390 2499 3027 4494 4579 5650 2390 Size: 109 528 1467 85 1071 3173 Fragments arranged by size: 3173 1467 1071 528 109 85 BsmI GAATG_Cn' Cuts at: 3944 4037 3944 Size: 93 6340 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2139 2390 2499 2746 3027 3752 Size: 399 274 75 251 109 247 281 725 Cuts at: 3752 4494 4579 5650 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 725 399 281 274 251 247 109 85 75 BspEI T'CCGG_A Cuts at: 6096 6096 Size: 6433 BspHI T'CATG_A Cuts at: 3555 4322 6052 3555 Size: 767 1730 3936 Fragments arranged by size: 3936 1730 767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3788 844 Size: 2944 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4320 1394 Size: 429 2497 3507 Fragments arranged by size: 3507 2497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2119 2841 3372 4928 5102 65 Size: 1183 871 722 531 1556 174 1396 Fragments arranged by size: 1556 1396 1183 871 722 531 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5077 5264 1700 Size: 110 3267 187 2869 Fragments arranged by size: 3267 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2469 3669 96 Size: 2373 1200 2860 Fragments arranged by size: 2860 2373 1200 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6433 BssSI C'ACGA_G Cuts at: 4493 5505 4493 Size: 1012 5421 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6433 BstBI TT'CG_AA Cuts at: 2007 5237 2007 Size: 3230 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6433 BstXI CCAn_nnnn'nTGG Cuts at: 3083 3586 3707 3083 Size: 503 121 5809 Fragments arranged by size: 5809 503 121 BstYI r'GATC_y Cuts at: 1972 2689 2908 3383 3763 4130 4515 4532 5984 Size: 717 219 475 380 367 385 17 1452 Cuts at: 5984 6070 6082 6428 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 717 475 385 380 367 346 219 86 17 12 BstZ17I GTA'TAC Cuts at: 2086 2086 Size: 6433 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5881 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3181 487 Size: 683 56 1955 3739 Fragments arranged by size: 3739 1955 683 56 BtsI GCAGTG_nn' Cuts at: 2859 3949 4743 4763 2859 Size: 1090 794 20 4529 Fragments arranged by size: 4529 1090 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6433 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6398 DraI TTT'AAA Cuts at: 2233 3737 2233 Size: 1504 4929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5440 809 Size: 507 4124 1802 Fragments arranged by size: 4124 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2925 3880 4769 Size: 54 1425 50 275 18 1095 955 889 Cuts at: 4769 6398 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1095 955 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3880 1537 Size: 275 2068 4090 Fragments arranged by size: 4090 2068 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2836 4363 1353 Size: 1483 1527 3423 Fragments arranged by size: 3423 1527 1483 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5020 5394 5540 387 Size: 1064 138 3431 374 146 1280 Fragments arranged by size: 3431 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2388 2497 2388 Size: 109 6324 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2703 2836 2940 3041 4496 5093 5880 Size: 393 1071 133 104 101 1455 597 787 Cuts at: 5880 6148 1239 Size: 268 1524 Fragments arranged by size: 1524 1455 1071 787 597 393 268 133 104 101 EcoNI CCTnn'n_nnAGG Cuts at: 2504 2504 Size: 6433 EcoO109I rG'GnC_Cy Cuts at: 2797 2797 Size: 6433 EcoRI G'AATT_C Cuts at: 3742 4120 3742 Size: 378 6055 EcoRV GAT'ATC Cuts at: 2551 2551 Size: 6433 FspI TGC'GCA Cuts at: 4939 4939 Size: 6433 HaeII r_GCGC'y Cuts at: 1678 5580 1678 Size: 3902 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2701 2733 3528 3560 5074 Size: 32 135 32 1129 32 795 32 1514 Cuts at: 5074 5106 5148 5180 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1514 1129 795 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2965 3622 3777 4023 669 Size: 988 281 1027 657 155 246 3079 Fragments arranged by size: 3079 1027 988 657 281 246 155 HindIII A'AGCT_T Cuts at: 748 2364 748 Size: 1616 4817 HpaI GTT'AAC Cuts at: 4023 4023 Size: 6433 KpnI G_GTAC'C Cuts at: 3758 3758 Size: 6433 MfeI C'AATT_G Cuts at: 3362 4032 3362 Size: 670 5763 MluI A'CGCG_T Cuts at: 6309 6309 Size: 6433 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5547 5731 1305 Size: 4242 184 2007 Fragments arranged by size: 4242 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2927 10 Size: 54 1425 343 1095 3516 Fragments arranged by size: 3516 1425 1095 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2666 2864 3081 3255 3584 4391 4750 Size: 648 1500 198 217 174 329 807 359 Cuts at: 4750 4909 518 Size: 159 2042 Fragments arranged by size: 2042 1500 807 648 359 329 217 198 174 159 MspA1I CmG'CkG Cuts at: 1606 3068 3849 4526 5674 5919 1606 Size: 1462 781 677 1148 245 2120 Fragments arranged by size: 2120 1462 1148 781 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5881 NdeI CA'TA_TG Cuts at: 387 387 Size: 6433 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6433 NotI GC'GGCC_GC Cuts at: 3880 3880 Size: 6433 NspI r_CATG'y Cuts at: 1132 1229 2332 3791 5336 1132 Size: 97 1103 1459 1545 2229 Fragments arranged by size: 2229 1545 1459 1103 97 PasI CC'CwG_GG Cuts at: 1348 2377 3691 1348 Size: 1029 1314 4090 Fragments arranged by size: 4090 1314 1029 PciI A'CATG_T Cuts at: 2328 5332 2328 Size: 3004 3429 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6433 PfoI T'CCnGG_A Cuts at: 2852 2852 Size: 6433 PmeI GTTT'AAAC Cuts at: 3737 3737 Size: 6433 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4502 4534 6015 6047 4502 Size: 32 1481 32 4888 Fragments arranged by size: 4888 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2797 2797 Size: 6433 PshAI GACnn'nnGTC Cuts at: 1613 2970 1613 Size: 1357 5076 PsiI TTA'TAA Cuts at: 4003 4003 Size: 6433 PstI C_TGCA'G Cuts at: 830 3785 830 Size: 2955 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6433 PvuII CAG'CTG Cuts at: 3068 3068 Size: 6433 SacI G_AGCT'C Cuts at: 2390 2499 2390 Size: 109 6324 SalI G'TCGA_C Cuts at: 1936 2963 3775 1936 Size: 1027 812 4594 Fragments arranged by size: 4594 1027 812 SanDI GG'GwC_CC Cuts at: 2797 2797 Size: 6433 SapI GCTCTTCn'nnn_ Cuts at: 2836 2836 Size: 6433 SbfI CC_TGCA'GG Cuts at: 3785 3785 Size: 6433 ScaI AGT'ACT Cuts at: 4681 6393 4681 Size: 1712 4721 SfcI C'TryA_G Cuts at: 826 1045 3781 4916 5597 5788 826 Size: 219 2736 1135 681 191 1471 Fragments arranged by size: 2736 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6433 SmaI CCC'GGG Cuts at: 1872 3760 1872 Size: 1888 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 3311 4537 5438 5700 820 Size: 197 926 468 900 1226 901 262 1553 Fragments arranged by size: 1553 1226 926 901 900 468 262 197 SnaBI TAC'GTA Cuts at: 493 3730 4224 493 Size: 3237 494 2702 Fragments arranged by size: 3237 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6433 SphI G_CATG'C Cuts at: 1132 3791 1132 Size: 2659 3774 SspI AAT'ATT Cuts at: 5 52 4357 5 Size: 47 4305 2081 Fragments arranged by size: 4305 2081 47 StuI AGG'CCT Cuts at: 1517 2054 1517 Size: 537 5896 StyI C'CwwG_G Cuts at: 513 1065 2307 3872 513 Size: 552 1242 1565 3074 Fragments arranged by size: 3074 1565 1242 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3042 3089 3478 3578 3704 4460 4477 Size: 1255 1462 47 389 100 126 756 17 Cuts at: 4477 4630 4815 5260 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1462 1255 756 445 389 185 153 126 100 47 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 2469 3132 Size: 274 80 33 51 1443 441 51 663 Cuts at: 3132 3669 4679 6391 96 Size: 537 1010 1712 138 Fragments arranged by size: 1712 1443 1010 663 537 441 274 138 80 51 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6393 6425 6393 Size: 32 6401 Tth111I GACn'n_nGTC Cuts at: 1316 3683 1316 Size: 2367 4066 XbaI T'CTAG_A Cuts at: 3769 3769 Size: 6433 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2217 3601 2217 Size: 1384 5049 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6433 XmaI C'CCGG_G Cuts at: 1870 3758 1870 Size: 1888 4545 XmnI GAAnn'nnTTC Cuts at: 4562 4562 Size: 6433 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4241 276 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI