Restriction map for
pFN21ASDA1247
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BamHI
BbsI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BseAI BsiHKAI
BsiWI BspDI BspEI BspHI BspLU11I
BssHII BstAPI BstBI BstYI ClaI
CpoI DraI Eam1105I EcoNI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MflI MluI MroI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SwaI XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 937 GTATAC GT^[AC][GT]AC
AccII 756 CGCG CG^CG
AlwI 779 GGATC GGATC(4/5)
Aor51HI 845 AGCGCT AGC^GCT
AspI 1825 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 9 CCCGAG C^[CT]CG[GA]G
BanI 2211 GGCGCC G^G[CT][GA]CC
BbeI 2211 GGCGCC GGCGC^C
BbvCI 2436 GCTGAGG CCTCAGC(-5/-2)
BcgI 11 CGAGCCTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1536 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1536 GCTCAGC GC^T[ACGT]AGC
BsaI 2562 GAGACC GGTCTC(1/5)
BsaWI 407 ACCGGA [AT]^CCGG[AT]
BsiEI 1180 CGGCCG CG[GA][CT]^CG
BsmI 2376 GAATGC GAATGC(1/-1)
BsoBI 9 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1850 TGTACA T^GTACA
BsrDI 1113 CATTGC GCAATG(2/0)
BsrGI 1850 TGTACA T^GTACA
Bst1107I 937 GTATAC GTA^TAC
BstUI 756 CGCG CG^CG
BstZ17I 937 GTATAC GTA^TAC
CelII 1536 GCTCAGC GC^T[ACGT]AGC
DraIII 516 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1180 CGGCCG C^GGCCG
EclXI 1180 CGGCCG C^GGCCG
Eco47III 845 AGCGCT AGC^GCT
Eco52I 1180 CGGCCG C^GGCCG
EcoRI 820 GAATTC G^AATTC
KasI 2211 GGCGCC G^GCGCC
MvnI 756 CGCG CG^CG
NaeI 1054 GCCGGC GCC^GGC
NarI 2211 GGCGCC GG^CGCC
NcoI 878 CCATGG C^CATGG
NdeI 1001 CATATG CA^TATG
NgoMIV 1054 GCCGGC G^CCGGC
PflFI 1825 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 159 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1330 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1860 CCTGCAGG CCTGCA^GG
SfiI 967 GGCCAGTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2211 GGCGCC GGC^GCC
SphI 2162 GCATGC GCATG^C
Sse8387I 1860 CCTGCAGG CCTGCA^GG
SspBI 1850 TGTACA T^GTACA
SspI 701 AATATT AAT^ATT
Tth111I 1825 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 159 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 820 GAATTC [GA]^AATT[CT]
2317 GAATTT
ApoI 820 GAATTC [GA]^AATT[CT]
2317 GAATTT
BbrPI 2154 CACGTG CAC^GTG
2175 CACGTG
BfaI 1816 CTAG C^TAG
2335 CTAG
BsmBI 883 GAGACG CGTCTC(1/5)
907 GAGACG
BsrBI 1611 CCGCTC CCGCTC(-3/-3)
1177 GAGCGG
BstEII 955 GGTTACC G^GT[ACGT]ACC
1948 GGTCACC
BstPI 955 GGTTACC G^GT[ACGT]ACC
1948 GGTCACC
DpnII 780 GATC ^GATC
2347 GATC
EcoO65I 955 GGTTACC G^GT[ACGT]ACC
1948 GGTCACC
EcoT14I 563 CCAAGG C^C[AT][AT]GG
878 CCATGG
MaeI 1816 CTAG C^TAG
2335 CTAG
MboI 780 GATC ^GATC
2347 GATC
NdeII 780 GATC ^GATC
2347 GATC
PmaCI 2154 CACGTG CAC^GTG
2175 CACGTG
PmlI 2154 CACGTG CAC^GTG
2175 CACGTG
PpuMI 1019 GGGTCCC [GA]G^G[AT]CC[CT]
1328 AGGACCT
PvuII 1369 CAGCTG CAG^CTG
2368 CAGCTG
Sau3AI 780 GATC ^GATC
2347 GATC
ScaI 1484 AGTACT AGT^ACT
2339 AGTACT
StyI 563 CCAAGG C^C[AT][AT]GG
878 CCATGG
XcmI 131 CCAACATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1607 CCATCCGCTCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AocI ApaI AvaII BalI BanII
BbvI BcnI BglI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstXI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV NspI PleI PstI
RsaI Sau96I ScrFI SfaNI SfcI
SmlI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I