Restriction map for
pFN21ASDA1247
(Linear) (Six-base) MAPSORT of: KIAA1247.seq Check: 6376 from: 1 to: 2610
KIAA1247.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 174 2610
Size: 174 2436
AccI GT'mk_AC
Cuts at: 0 938 2610
Size: 938 1672
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1435 1728 1975 2610
Size: 1435 293 247 635
Fragments arranged by size:
1435 635 293 247
AfeI AGC'GCT
Cuts at: 0 847 2610
Size: 847 1763
AflIII A'CryG_T
Cuts at: 0 926 2153 2264 2418 2610
Size: 926 1227 111 154 192
Fragments arranged by size:
1227 926 192 154 111
AleI CACnn'nnGTG
Cuts at: 0 258 1803 2610
Size: 258 1545 807
Fragments arranged by size:
1545 807 258
ApaI G_GGCC'C
Cuts at: 0 7 800 1042 1438 1710 2610
Size: 7 793 242 396 272 900
Fragments arranged by size:
900 793 396 272 242 7
ApoI r'AATT_y
Cuts at: 0 820 2317 2610
Size: 820 1497 293
Fragments arranged by size:
1497 820 293
AvaI C'yCGr_G
Cuts at: 0 9 2610
Size: 9 2601
BanI G'GyrC_C
Cuts at: 0 2211 2610
Size: 2211 399
BanII G_rGCy'C
Cuts at: 0 7 800 1042 1438 1464 1710 2610
Size: 7 793 242 396 26 246 900
Fragments arranged by size:
900 793 396 246 242 26 7
BbeI G_GCGC'C
Cuts at: 0 2215 2610
Size: 2215 395
BbvCI CC'TCA_GC
Cuts at: 0 2437 2610
Size: 2437 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 2610
Size: 34 2576
BglI GCCn_nnn'nGGC
Cuts at: 0 95 351 974 2610
Size: 95 256 623 1636
Fragments arranged by size:
1636 623 256 95
BlpI GC'TnA_GC
Cuts at: 0 1537 2610
Size: 1537 1073
Bme1580I G_kGCm'C
Cuts at: 0 7 800 1042 1438 1481 1710 2610
Size: 7 793 242 396 43 229 900
Fragments arranged by size:
900 793 396 242 229 43 7
BmgBI CAC'GTC
Cuts at: 0 617 2610
Size: 617 1993
BmrI ACTGGGnnnn_n'
Cuts at: 0 2236 2590 2610
Size: 2236 354 20
Fragments arranged by size:
2236 354 20
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 354 2610
Size: 354 2256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1775 2022 2610
Size: 820 955 247 588
Fragments arranged by size:
955 820 588 247
Bpu10I CC'TnA_GC
Cuts at: 0 2437 2610
Size: 2437 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 2008 2116 2610
Size: 1144 864 108 494
Fragments arranged by size:
1144 864 494 108
BsaI GGTCTCn'nnnn_
Cuts at: 0 2556 2610
Size: 2556 54
BsaAI yAC'GTr
Cuts at: 0 456 929 1032 1650 2156 2177 2267 2421
Size: 456 473 103 618 506 21 90 154
Cuts at: 2421 2610
Size: 189
Fragments arranged by size:
618 506 473 456 189 154 103 90
21
BsaHI Gr'CG_yC
Cuts at: 0 1286 1400 1551 2212 2610
Size: 1286 114 151 661 398
Fragments arranged by size:
1286 661 398 151 114
BsaWI w'CCGG_w
Cuts at: 0 407 2610
Size: 407 2203
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 2020 2050 2610
Size: 433 30 1557 30 560
Fragments arranged by size:
1557 560 433 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 267 330 1309 2454 2610
Size: 267 63 979 1145 156
Fragments arranged by size:
1145 979 267 156 63
BseYI C'CCAG_C
Cuts at: 0 54 171 1371 1446 1670 2006 2600 2610
Size: 54 117 1200 75 224 336 594 10
Fragments arranged by size:
1200 594 336 224 117 75 54 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1494 1870 2178 2229 2610
Size: 1494 376 308 51 381
Fragments arranged by size:
1494 381 376 308 51
BsiEI CG_ry'CG
Cuts at: 0 1183 2610
Size: 1183 1427
BsmI GAATG_Cn'
Cuts at: 0 2382 2610
Size: 2382 228
BsmBI CGTCTCn'nnnn_
Cuts at: 0 877 901 2610
Size: 877 24 1709
Fragments arranged by size:
1709 877 24
Bsp1286I G_dGCh'C
Cuts at: 0 7 800 1042 1438 1464 1481 1710 2610
Size: 7 793 242 396 26 17 229 900
Fragments arranged by size:
900 793 396 242 229 26 17 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 174 1140 2610
Size: 89 85 966 1470
Fragments arranged by size:
1470 966 89 85
BsrBI CCG'CTC
Cuts at: 0 1179 1613 2610
Size: 1179 434 997
Fragments arranged by size:
1179 997 434
BsrDI GCAATG_nn'
Cuts at: 0 1112 2610
Size: 1112 1498
BsrFI r'CCGG_y
Cuts at: 0 289 1054 1182 2610
Size: 289 765 128 1428
Fragments arranged by size:
1428 765 289 128
BsrGI T'GTAC_A
Cuts at: 0 1850 2610
Size: 1850 760
BssSI C'ACGA_G
Cuts at: 0 16 364 1474 1897 2610
Size: 16 348 1110 423 713
Fragments arranged by size:
1110 713 423 348 16
BstEII G'GTnAC_C
Cuts at: 0 955 1948 2610
Size: 955 993 662
Fragments arranged by size:
993 955 662
BstXI CCAn_nnnn'nTGG
Cuts at: 0 467 816 1107 1446 1629 2580 2610
Size: 467 349 291 339 183 951 30
Fragments arranged by size:
951 467 349 339 291 183 30
BstZ17I GTA'TAC
Cuts at: 0 939 2610
Size: 939 1671
Bsu36I CC'TnA_GG
Cuts at: 0 683 1712 1933 2610
Size: 683 1029 221 677
Fragments arranged by size:
1029 683 677 221
BtgI C'CryG_G
Cuts at: 0 675 878 951 1403 2610
Size: 675 203 73 452 1207
Fragments arranged by size:
1207 675 452 203 73
BtsI GCAGTG_nn'
Cuts at: 0 1393 2387 2467 2610
Size: 1393 994 80 143
Fragments arranged by size:
1393 994 143 80
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1716 1751 2610
Size: 1716 35 859
Fragments arranged by size:
1716 859 35
DraIII CAC_nnn'GTG
Cuts at: 0 521 2610
Size: 521 2089
DrdI GACnn_nn'nnGTC
Cuts at: 0 861 1018 2401 2610
Size: 861 157 1383 209
Fragments arranged by size:
1383 861 209 157
EaeI y'GGCC_r
Cuts at: 0 966 1180 1544 1619 1699 1966 2548 2610
Size: 966 214 364 75 80 267 582 62
Fragments arranged by size:
966 582 364 267 214 80 75 62
EagI C'GGCC_G
Cuts at: 0 1180 2610
Size: 1180 1430
EarI CTCTTCn'nnn_
Cuts at: 0 718 1570 2314 2554 2610
Size: 718 852 744 240 56
Fragments arranged by size:
852 744 718 240 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 115 2610
Size: 115 2495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1435 1728 1775 1975 2022 2610
Size: 820 615 293 47 200 47 588
Fragments arranged by size:
820 615 588 293 200 47 47
EcoO109I rG'GnC_Cy
Cuts at: 0 4 796 1020 1038 1039 1329 1434 1435
Size: 4 792 224 18 1 290 105 1
Cuts at: 1435 1706 1707 2061 2230 2610
Size: 271 1 354 169 380
Fragments arranged by size:
792 380 354 290 271 224 169 105
18 4 1 1 1
EcoRI G'AATT_C
Cuts at: 0 820 2610
Size: 820 1790
HaeII r_GCGC'y
Cuts at: 0 849 2139 2215 2255 2610
Size: 849 1290 76 40 355
Fragments arranged by size:
1290 849 355 76 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1135 1167 2610
Size: 1135 32 1443
Fragments arranged by size:
1443 1135 32
KasI G'GCGC_C
Cuts at: 0 2211 2610
Size: 2211 399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 505 578 1493 2610
Size: 462 43 73 915 1117
Fragments arranged by size:
1117 915 462 73 43
MscI TGG'CCA
Cuts at: 0 1621 1701 2550 2610
Size: 1621 80 849 60
Fragments arranged by size:
1621 849 80 60
MslI CAynn'nnrTG
Cuts at: 0 142 184 256 258 814 829 865 1627
Size: 142 42 72 2 556 15 36 762
Cuts at: 1627 1792 1803 2610
Size: 165 11 807
Fragments arranged by size:
807 762 556 165 142 72 42 36
15 11 2
MspA1I CmG'CkG
Cuts at: 0 840 1371 1523 2370 2610
Size: 840 531 152 847 240
Fragments arranged by size:
847 840 531 240 152
NaeI GCC'GGC
Cuts at: 0 1056 2610
Size: 1056 1554
NarI GG'CG_CC
Cuts at: 0 2212 2610
Size: 2212 398
NcoI C'CATG_G
Cuts at: 0 878 2610
Size: 878 1732
NdeI CA'TA_TG
Cuts at: 0 1002 2610
Size: 1002 1608
NgoMIV G'CCGG_C
Cuts at: 0 1054 2610
Size: 1054 1556
NspI r_CATG'y
Cuts at: 0 834 900 2166 2610
Size: 834 66 1266 444
Fragments arranged by size:
1266 834 444 66
PflMI CCAn_nnn'nTGG
Cuts at: 0 165 2610
Size: 165 2445
PfoI T'CCnGG_A
Cuts at: 0 1106 2610
Size: 1106 1504
PmlI CAC'GTG
Cuts at: 0 2156 2177 2610
Size: 2156 21 433
Fragments arranged by size:
2156 433 21
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 2610
Size: 325 32 2253
Fragments arranged by size:
2253 325 32
PpuMI rG'GwC_Cy
Cuts at: 0 1020 1329 2610
Size: 1020 309 1281
Fragments arranged by size:
1281 1020 309
PshAI GACnn'nnGTC
Cuts at: 0 1334 2610
Size: 1334 1276
PsiI TTA'TAA
Cuts at: 0 509 2610
Size: 509 2101
PspOMI G'GGCC_C
Cuts at: 0 3 796 1038 1434 1706 2610
Size: 3 793 242 396 272 904
Fragments arranged by size:
904 793 396 272 242 3
PstI C_TGCA'G
Cuts at: 0 1865 1916 2144 2610
Size: 1865 51 228 466
Fragments arranged by size:
1865 466 228 51
PvuII CAG'CTG
Cuts at: 0 1371 2370 2610
Size: 1371 999 240
Fragments arranged by size:
1371 999 240
SanDI GG'GwC_CC
Cuts at: 0 1020 2610
Size: 1020 1590
SbfI CC_TGCA'GG
Cuts at: 0 1865 2610
Size: 1865 745
ScaI AGT'ACT
Cuts at: 0 1486 2341 2610
Size: 1486 855 269
Fragments arranged by size:
1486 855 269
SfcI C'TryA_G
Cuts at: 0 1861 1912 2140 2610
Size: 1861 51 228 470
Fragments arranged by size:
1861 470 228 51
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 974 2610
Size: 974 1636
SfoI GGC'GCC
Cuts at: 0 2213 2610
Size: 2213 397
SmlI C'TyrA_G
Cuts at: 0 1159 2023 2131 2610
Size: 1159 864 108 479
Fragments arranged by size:
1159 864 479 108
SphI G_CATG'C
Cuts at: 0 2166 2610
Size: 2166 444
SspI AAT'ATT
Cuts at: 0 703 2610
Size: 703 1907
StuI AGG'CCT
Cuts at: 0 1121 1507 1655 1760 1867 2171 2610
Size: 1121 386 148 105 107 304 439
Fragments arranged by size:
1121 439 386 304 148 107 105
StyI C'CwwG_G
Cuts at: 0 563 878 2610
Size: 563 315 1732
Fragments arranged by size:
1732 563 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 46 270 1648 2020 2200 2610
Size: 46 224 1378 372 180 410
Fragments arranged by size:
1378 410 372 224 180 46
TatI w'GTAC_w
Cuts at: 0 1484 1577 1850 2339 2610
Size: 1484 93 273 489 271
Fragments arranged by size:
1484 489 273 271 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 698 730 2020 2052 2068 2100 2610
Size: 698 32 1290 32 16 32 510
Fragments arranged by size:
1290 698 510 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 0 1828 2610
Size: 1828 782
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 138 1614 2610
Size: 138 1476 996
Fragments arranged by size:
1476 996 138
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI ApaI ApoI
AvaI BanI BanII BbeI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtsI CspCI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PflMI PfoI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII
SanDI SbfI ScaI SfcI SfiI SfoI SmlI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BciVI BclI BfrBI BglII BmtI BsaBI BsiHKAI
BsiWI BspEI BspHI BssHII BstAPI BstBI BstYI BtgZI
ClaI DraI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NheI
NotI NruI NsiI PacI PasI PciI PmeI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SexAI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI XbaI
XhoI XmaI XmnI ZraI