(Circular) (Six-base) MAPSORT of: pFN21ASDA1244.seq Check: 2755 from: 1 to: 10246 pFN21ASDA1244 10246 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3310 5998 3310 Size: 2688 7558 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4827 8056 278 Size: 53 83 186 936 3291 3229 2468 Fragments arranged by size: 3291 3229 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2679 2748 7589 1937 Size: 742 69 4841 4594 Fragments arranged by size: 4841 4594 742 69 Acc65I G'GTAC_C Cuts at: 7567 7567 Size: 10246 AclI AA'CG_TT Cuts at: 1563 8373 8746 1563 Size: 6810 373 3063 Fragments arranged by size: 6810 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2411 3017 3266 3720 4078 4460 5270 5714 Size: 779 606 249 454 358 382 810 444 Cuts at: 5714 5899 6398 6410 6965 7184 7231 8309 9693 Size: 185 499 12 555 219 47 1078 1384 Cuts at: 9693 9961 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1078 810 779 606 555 499 454 444 382 358 268 249 219 185 47 12 AfeI AGC'GCT Cuts at: 4156 4156 Size: 10246 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10049 AflIII A'CryG_T Cuts at: 1203 2886 4045 4047 4678 9145 10122 1203 Size: 1683 1159 2 631 4467 977 1327 Fragments arranged by size: 4467 1683 1327 1159 977 631 2 AgeI A'CCGG_T Cuts at: 2958 5351 9077 2958 Size: 2393 3726 4127 Fragments arranged by size: 4127 3726 2393 AhdI GACnn_n'nnGTC Cuts at: 2899 8975 2899 Size: 6076 4170 AleI CACnn'nnGTG Cuts at: 1166 3902 1166 Size: 2736 7510 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3132 1184 Size: 1948 8298 AlwNI CAG_nnn'CTG Cuts at: 3046 3190 3928 4252 5199 5399 7989 9561 3046 Size: 144 738 324 947 200 2590 1572 3731 Fragments arranged by size: 3731 2590 1572 947 738 324 200 144 ApaI G_GGCC'C Cuts at: 5798 5798 Size: 10246 ApaLI G'TGCA_C Cuts at: 8303 9459 8303 Size: 1156 9090 ApoI r'AATT_y Cuts at: 1493 7555 7781 7933 10042 1493 Size: 6062 226 152 2109 1697 Fragments arranged by size: 6062 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 8800 160 Size: 872 7768 1606 Fragments arranged by size: 7768 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10246 AvaI C'yCGr_G Cuts at: 1870 1943 2555 2582 2872 2940 4175 4196 6371 Size: 73 612 27 290 68 1235 21 2175 Cuts at: 6371 6622 6915 7259 7571 1870 Size: 251 293 344 312 4545 Fragments arranged by size: 4545 2175 1235 612 344 312 293 290 251 73 68 27 21 AvrII C'CTAG_G Cuts at: 2950 2950 Size: 10246 BamHI G'GATC_C Cuts at: 7000 7576 7943 7000 Size: 576 367 9303 Fragments arranged by size: 9303 576 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2312 3289 4073 4103 6413 Size: 325 217 627 525 977 784 30 2310 Cuts at: 6413 7567 9022 618 Size: 1154 1455 1842 Fragments arranged by size: 2310 1842 1455 1154 977 784 627 525 325 217 30 BanII G_rGCy'C Cuts at: 1391 2126 2328 3375 3671 4190 5155 5798 7310 Size: 735 202 1047 296 519 965 643 1512 Cuts at: 7310 7565 1391 Size: 255 4072 Fragments arranged by size: 4072 1512 1047 965 735 643 519 296 255 202 BbeI G_GCGC'C Cuts at: 3293 6417 3293 Size: 3124 7122 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3340 5287 6594 928 Size: 971 1441 1947 1307 4580 Fragments arranged by size: 4580 1947 1441 1307 971 BbvCI CC'TCA_GC Cuts at: 3176 3814 3998 4577 3176 Size: 638 184 579 8845 Fragments arranged by size: 8845 638 579 184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3733 3767 6238 6272 8437 8471 1903 Size: 34 1796 34 2471 34 2165 34 3678 Fragments arranged by size: 3678 2471 2165 1796 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8138 9348 8138 Size: 1210 9036 BclI T'GATC_A Cuts at: 1548 2842 5679 1548 Size: 1294 2837 6115 Fragments arranged by size: 6115 2837 1294 BfrBI ATG'CAT Cuts at: 2253 2253 Size: 10246 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3830 5726 6272 Size: 107 122 71 631 605 2158 1896 546 Cuts at: 6272 8857 136 Size: 2585 1525 Fragments arranged by size: 2585 2158 1896 1525 631 605 546 122 107 71 BglII A'GATC_T Cuts at: 10241 10241 Size: 10246 BlpI GC'TnA_GC Cuts at: 2327 2651 3779 6668 7663 2327 Size: 324 1128 2889 995 4910 Fragments arranged by size: 4910 2889 1128 995 324 Bme1580I G_kGCm'C Cuts at: 1790 2317 4076 4915 5140 5798 5839 6655 8307 Size: 527 1759 839 225 658 41 816 1652 Cuts at: 8307 9463 1790 Size: 1156 2573 Fragments arranged by size: 2573 1759 1652 1156 839 816 658 527 225 41 BmgBI CAC'GTC Cuts at: 1318 2887 3976 1318 Size: 1569 1089 7588 Fragments arranged by size: 7588 1569 1089 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3879 5111 5581 Size: 416 53 83 399 24 2462 1232 470 Cuts at: 5581 6022 7178 7977 8935 442 Size: 441 1156 799 958 1753 Fragments arranged by size: 2462 1753 1232 1156 958 799 470 441 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10246 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2078 3804 2078 Size: 1726 8520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2021 3950 4031 4100 4648 4980 5402 5734 Size: 782 1929 81 69 548 332 422 332 Cuts at: 5734 6167 6255 6794 7406 8906 1239 Size: 433 88 539 612 1500 2579 Fragments arranged by size: 2579 1929 1500 782 612 548 539 433 422 332 332 88 81 69 Bpu10I CC'TnA_GC Cuts at: 3133 3176 3814 3998 4226 4577 6938 7291 3133 Size: 43 638 184 228 351 2361 353 6088 Fragments arranged by size: 6088 2361 638 353 351 228 184 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2365 2402 2600 4512 5849 8371 9236 9534 2365 Size: 37 198 1912 1337 2522 865 298 3077 Fragments arranged by size: 3077 2522 1912 1337 865 298 198 37 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4891 8909 882 Size: 617 3392 4018 2219 Fragments arranged by size: 4018 3392 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4048 4069 4615 7465 7543 8037 493 Size: 711 2844 21 546 2850 78 494 2702 Fragments arranged by size: 2850 2844 2702 711 546 494 78 21 BsaBI GATnn'nnATC Cuts at: 10240 10240 Size: 10246 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3290 3735 4824 6414 Size: 53 83 186 936 1757 445 1089 1590 Cuts at: 6414 8053 8435 9070 275 Size: 1639 382 635 1451 Fragments arranged by size: 1757 1639 1590 1451 1089 936 635 445 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2958 5351 8679 9077 9351 9498 9909 2958 Size: 2393 3328 398 274 147 411 3295 Fragments arranged by size: 3328 3295 2393 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3517 3547 1178 Size: 30 165 30 2114 30 7877 Fragments arranged by size: 7877 2114 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 2399 2518 3047 3104 3227 3383 5507 Size: 1021 192 119 529 57 123 156 2124 Cuts at: 5507 5825 5947 6803 1186 Size: 318 122 856 4629 Fragments arranged by size: 4629 2124 1021 856 529 318 192 156 123 122 119 57 BseYI C'CCAG_C Cuts at: 2076 2153 2308 5140 5884 6003 6564 6977 7004 Size: 77 155 2832 744 119 561 413 27 Cuts at: 7004 7209 7504 9449 9945 2076 Size: 205 295 1945 496 2377 Fragments arranged by size: 2832 2377 1945 744 561 496 413 295 205 155 119 77 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2284 2717 3204 3344 3947 3968 6704 Size: 573 548 433 487 140 603 21 2736 Cuts at: 6704 1163 Size: 4705 Fragments arranged by size: 4705 2736 603 573 548 487 433 140 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3281 6510 7696 8457 9485 1540 Size: 275 186 1280 3229 1186 761 1028 2301 Fragments arranged by size: 3229 2301 1280 1186 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2037 2328 3375 3390 3671 4086 4203 4232 4353 Size: 291 1047 15 281 415 117 29 121 Cuts at: 4353 5637 6035 6924 7310 8307 8392 9463 2037 Size: 1284 398 889 386 997 85 1071 2820 Fragments arranged by size: 2820 1284 1071 1047 997 889 415 398 386 291 281 121 117 85 29 15 BsmI GAATG_Cn' Cuts at: 4573 7036 7240 7757 7850 4573 Size: 2463 204 517 93 6969 Fragments arranged by size: 6969 2463 517 204 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2138 2639 7323 2138 Size: 501 4684 5061 Fragments arranged by size: 5061 4684 501 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2126 2317 2328 3375 3390 3671 Size: 399 247 89 191 11 1047 15 281 Cuts at: 3671 4076 4086 4190 4203 4232 4353 4915 5140 Size: 405 10 104 13 29 121 562 225 Cuts at: 5140 5155 5637 5798 5839 6035 6655 6924 7310 Size: 15 482 161 41 196 620 269 386 Cuts at: 7310 7565 8307 8392 9463 1391 Size: 255 742 85 1071 2174 Fragments arranged by size: 2174 1071 1047 742 620 562 482 405 399 386 281 269 255 247 225 196 191 161 121 104 89 85 41 29 15 15 13 11 10 BspEI T'CCGG_A Cuts at: 9909 9909 Size: 10246 BspHI T'CATG_A Cuts at: 3312 4320 8135 9865 3312 Size: 1008 3815 1730 3693 Fragments arranged by size: 3815 3693 1730 1008 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2868 3310 5889 5998 6333 6972 7601 844 Size: 2024 442 2579 109 335 639 629 3489 Fragments arranged by size: 3489 2579 2024 639 629 442 335 109 BsrBI CCG'CTC Cuts at: 1394 1823 3104 3730 4326 7290 8133 1394 Size: 429 1281 626 596 2964 843 3507 Fragments arranged by size: 3507 2964 1281 843 626 596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4509 8741 8915 65 Size: 1183 3261 4232 174 1396 Fragments arranged by size: 4232 3261 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2958 5267 5351 6950 8890 9077 1700 Size: 110 1148 2309 84 1599 1940 187 2869 Fragments arranged by size: 2869 2309 1940 1599 1148 187 110 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10246 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10246 BssSI C'ACGA_G Cuts at: 8306 9318 8306 Size: 1012 9234 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 794 Size: 1574 8672 BstBI TT'CG_AA Cuts at: 2007 9050 2007 Size: 7043 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10246 BstXI CCAn_nnnn'nTGG Cuts at: 3679 3679 Size: 10246 BstYI r'GATC_y Cuts at: 1972 2212 2771 4881 7000 7344 7576 7943 8328 Size: 240 559 2110 2119 344 232 367 385 Cuts at: 8328 8345 9797 9883 9895 10241 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 2119 2110 1977 1452 559 385 367 346 344 240 232 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5192 5192 Size: 10246 BtgI C'CryG_G Cuts at: 513 1065 2421 2483 3256 4306 4977 5414 5997 Size: 552 1356 62 773 1050 671 437 583 Cuts at: 5997 7053 513 Size: 1056 3706 Fragments arranged by size: 3706 1356 1056 1050 773 671 583 552 437 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4113 4434 6455 487 Size: 683 56 2887 321 2021 4278 Fragments arranged by size: 4278 2887 2021 683 321 56 BtsI GCAGTG_nn' Cuts at: 3223 3496 3966 5311 5511 6575 7437 7762 8556 Size: 273 470 1345 200 1064 862 325 794 Cuts at: 8556 8576 3223 Size: 20 4893 Fragments arranged by size: 4893 1345 1064 862 794 470 325 273 200 20 ClaI AT'CG_AT Cuts at: 1554 3627 1554 Size: 2073 8173 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10211 DraI TTT'AAA Cuts at: 7550 7550 Size: 10246 DraIII CAC_nnn'GTG Cuts at: 2056 2086 2484 4651 6030 2056 Size: 30 398 2167 1379 6272 Fragments arranged by size: 6272 2167 1379 398 30 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4838 9253 809 Size: 507 3522 4415 1802 Fragments arranged by size: 4415 3522 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3278 3502 4040 Size: 54 1425 50 275 18 1448 224 538 Cuts at: 4040 4338 5214 5727 6507 7693 8582 10211 8 Size: 298 876 513 780 1186 889 1629 43 Fragments arranged by size: 1629 1448 1425 1186 889 876 780 538 513 298 275 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3278 6507 7693 1537 Size: 275 1466 3229 1186 4090 Fragments arranged by size: 4090 3229 1466 1186 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2922 3858 4781 6204 6632 8176 1353 Size: 1569 936 923 1423 428 1544 3423 Fragments arranged by size: 3423 1569 1544 1423 936 923 428 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3026 4547 5534 8833 9207 9353 Size: 1064 138 1437 1521 987 3299 374 146 Cuts at: 9353 387 Size: 1280 Fragments arranged by size: 3299 1521 1437 1280 1064 987 374 146 138 EcoICRI GAG'CTC Cuts at: 2326 3373 3669 7308 2326 Size: 1047 296 3639 5264 Fragments arranged by size: 5264 3639 1047 296 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2021 2411 3017 3266 3720 3950 4031 Size: 393 389 390 606 249 454 230 81 Cuts at: 4031 4078 4100 4460 4648 4980 5270 5402 5714 Size: 47 22 360 188 332 290 132 312 Cuts at: 5714 5734 5899 6167 6255 6398 6410 6794 6965 Size: 20 165 268 88 143 12 384 171 Cuts at: 6965 7184 7231 7406 8309 8906 9693 9961 1239 Size: 219 47 175 903 597 787 268 1524 Fragments arranged by size: 1524 903 787 606 597 454 393 390 389 384 360 332 312 290 268 268 249 230 219 188 175 171 165 143 132 88 81 47 47 22 20 12 EcoNI CCTnn'n_nnAGG Cuts at: 4652 6533 4652 Size: 1881 8365 EcoO109I rG'GnC_Cy Cuts at: 2150 2763 4171 4264 5698 5795 5878 6224 6526 Size: 613 1408 93 1434 97 83 346 302 Cuts at: 6526 6961 7172 7256 7284 2150 Size: 435 211 84 28 5112 Fragments arranged by size: 5112 1434 1408 613 435 346 302 211 97 93 84 83 28 EcoRI G'AATT_C Cuts at: 7555 7933 7555 Size: 378 9868 EcoRV GAT'ATC Cuts at: 5662 5662 Size: 10246 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2132 5719 6153 2132 Size: 3587 434 6225 Fragments arranged by size: 6225 3587 434 FspI TGC'GCA Cuts at: 8752 8752 Size: 10246 HaeII r_GCGC'y Cuts at: 1678 3027 3293 4158 4222 4545 5200 6417 9393 Size: 1349 266 865 64 323 655 1217 2976 Cuts at: 9393 1678 Size: 2531 Fragments arranged by size: 2976 2531 1349 1217 865 655 323 266 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2338 2370 2834 2866 2885 Size: 32 135 32 766 32 464 32 19 Cuts at: 2885 2917 3133 3165 3620 3652 4091 4123 5567 Size: 32 216 32 455 32 439 32 1444 Cuts at: 5567 5599 5837 5869 8887 8919 8961 8993 1373 Size: 32 238 32 3018 32 42 32 2626 Fragments arranged by size: 3018 2626 1444 766 464 455 439 238 216 135 42 32 32 32 32 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 7590 7836 669 Size: 988 281 5652 246 3079 Fragments arranged by size: 5652 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2271 5016 5644 748 Size: 1523 2745 628 5350 Fragments arranged by size: 5350 2745 1523 628 HpaI GTT'AAC Cuts at: 7836 7836 Size: 10246 KasI G'GCGC_C Cuts at: 3289 6413 3289 Size: 3124 7122 KpnI G_GTAC'C Cuts at: 7571 7571 Size: 10246 MfeI C'AATT_G Cuts at: 5407 7845 5407 Size: 2438 7808 MluI A'CGCG_T Cuts at: 10122 10122 Size: 10246 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2883 2907 4323 4804 9360 9544 1305 Size: 1578 24 1416 481 4556 184 2007 Fragments arranged by size: 4556 2007 1578 1416 481 184 24 MscI TGG'CCA Cuts at: 10 64 1489 1832 3504 4042 5216 5729 10 Size: 54 1425 343 1672 538 1174 513 4527 Fragments arranged by size: 4527 1672 1425 1174 538 513 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3458 3743 3902 4764 6296 6758 7535 Size: 648 2292 285 159 862 1532 462 777 Cuts at: 7535 8204 8563 8722 518 Size: 669 359 159 2042 Fragments arranged by size: 2292 2042 1532 862 777 669 648 462 359 285 159 159 MspA1I CmG'CkG Cuts at: 1606 2448 2455 3218 3333 3508 3836 4308 4438 Size: 842 7 763 115 175 328 472 130 Cuts at: 4438 4804 5389 5396 5792 5901 5919 7195 7453 Size: 366 585 7 396 109 18 1276 258 Cuts at: 7453 7662 8339 9487 9732 1606 Size: 209 677 1148 245 2120 Fragments arranged by size: 2120 1276 1148 842 763 677 585 472 396 366 328 258 245 209 175 130 115 109 18 7 7 NaeI GCC'GGC Cuts at: 5269 5269 Size: 10246 NarI GG'CG_CC Cuts at: 3290 6414 3290 Size: 3124 7122 NcoI C'CATG_G Cuts at: 513 1065 2421 4977 5997 7053 513 Size: 552 1356 2556 1020 1056 3706 Fragments arranged by size: 3706 2556 1356 1056 1020 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10246 NgoMIV G'CCGG_C Cuts at: 5267 5267 Size: 10246 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10246 NotI GC'GGCC_GC Cuts at: 7693 7693 Size: 10246 NsiI A_TGCA'T Cuts at: 2255 2255 Size: 10246 NspI r_CATG'y Cuts at: 1132 1229 2253 3403 4682 7604 9149 1132 Size: 97 1024 1150 1279 2922 1545 2229 Fragments arranged by size: 2922 2229 1545 1279 1150 1024 97 PasI CC'CwG_GG Cuts at: 1348 7176 1348 Size: 5828 4418 PciI A'CATG_T Cuts at: 4678 9145 4678 Size: 4467 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 3511 3650 5492 6469 1108 Size: 2403 139 1842 977 4885 Fragments arranged by size: 4885 2403 1842 977 139 PfoI T'CCnGG_A Cuts at: 4260 4272 6232 4260 Size: 12 1960 8274 Fragments arranged by size: 8274 1960 12 PmeI GTTT'AAAC Cuts at: 7550 7550 Size: 10246 PmlI CAC'GTG Cuts at: 4048 4615 7465 4048 Size: 567 2850 6829 Fragments arranged by size: 6829 2850 567 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3104 3136 8315 8347 9828 9860 3104 Size: 32 5179 32 1481 32 3490 Fragments arranged by size: 5179 3490 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2150 2763 4171 4264 5698 5878 6526 6961 7172 Size: 613 1408 93 1434 180 648 435 211 Cuts at: 7172 2150 Size: 5224 Fragments arranged by size: 5224 1434 1408 648 613 435 211 180 93 PshAI GACnn'nnGTC Cuts at: 1613 4681 4724 5352 1613 Size: 3068 43 628 6507 Fragments arranged by size: 6507 3068 628 43 PsiI TTA'TAA Cuts at: 7816 7816 Size: 10246 PspOMI G'GGCC_C Cuts at: 5794 5794 Size: 10246 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 7031 7063 7031 Size: 32 10214 PstI C_TGCA'G Cuts at: 830 2756 3111 7598 830 Size: 1926 355 4487 3478 Fragments arranged by size: 4487 3478 1926 355 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10246 PvuII CAG'CTG Cuts at: 3218 3508 3836 4438 4804 5389 7453 3218 Size: 290 328 602 366 585 2064 6011 Fragments arranged by size: 6011 2064 602 585 366 328 290 SacI G_AGCT'C Cuts at: 2328 3375 3671 7310 2328 Size: 1047 296 3639 5264 Fragments arranged by size: 5264 3639 1047 296 SacII CC_GC'GG Cuts at: 4309 4309 Size: 10246 SalI G'TCGA_C Cuts at: 1936 7588 1936 Size: 5652 4594 SanDI GG'GwC_CC Cuts at: 4171 7172 4171 Size: 3001 7245 SapI GCTCTTCn'nnn_ Cuts at: 2922 2922 Size: 10246 SbfI CC_TGCA'GG Cuts at: 7598 7598 Size: 10246 ScaI AGT'ACT Cuts at: 2849 5981 8494 10206 2849 Size: 3132 2513 1712 2889 Fragments arranged by size: 3132 2889 2513 1712 SexAI A'CCwGG_T Cuts at: 4647 4833 4959 4647 Size: 186 126 9934 Fragments arranged by size: 9934 186 126 SfcI C'TryA_G Cuts at: 826 1045 2752 3107 3268 3525 7594 8729 9410 Size: 219 1707 355 161 257 4069 1135 681 Cuts at: 9410 9601 826 Size: 191 1471 Fragments arranged by size: 4069 1707 1471 1135 681 355 257 219 191 161 SfoI GGC'GCC Cuts at: 3291 6415 3291 Size: 3124 7122 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10246 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10246 SmaI CCC'GGG Cuts at: 1872 2942 6373 6917 7573 1872 Size: 1070 3431 544 656 4545 Fragments arranged by size: 4545 3431 1070 656 544 SmlI C'TyrA_G Cuts at: 820 1017 1943 2344 2381 2555 2615 4527 5864 Size: 197 926 401 37 174 60 1912 1337 Cuts at: 5864 8350 9251 9513 820 Size: 2486 901 262 1553 Fragments arranged by size: 2486 1912 1553 1337 926 901 401 262 197 174 60 37 SnaBI TAC'GTA Cuts at: 493 7543 8037 493 Size: 7050 494 2702 Fragments arranged by size: 7050 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 10246 SphI G_CATG'C Cuts at: 1132 2253 7604 1132 Size: 1121 5351 3774 Fragments arranged by size: 5351 3774 1121 SspI AAT'ATT Cuts at: 5 52 5247 8170 5 Size: 47 5195 2923 2081 Fragments arranged by size: 5195 2923 2081 47 StuI AGG'CCT Cuts at: 1517 3054 3359 4008 1517 Size: 1537 305 649 7755 Fragments arranged by size: 7755 1537 649 305 StyI C'CwwG_G Cuts at: 513 1065 2421 2950 4165 4341 4416 4977 5997 Size: 552 1356 529 1215 176 75 561 1020 Cuts at: 5997 7053 7225 7685 513 Size: 1056 172 460 3074 Fragments arranged by size: 3074 1356 1215 1056 1020 561 552 529 460 176 172 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2258 2300 3482 4548 6041 6688 7103 Size: 1255 678 42 1182 1066 1493 647 415 Cuts at: 7103 7484 8273 8290 8443 8628 9073 325 Size: 381 789 17 153 185 445 1498 Fragments arranged by size: 1498 1493 1255 1182 1066 789 678 647 445 415 381 185 153 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2847 5979 8492 Size: 274 80 33 51 1443 870 3132 2513 Cuts at: 8492 10204 96 Size: 1712 138 Fragments arranged by size: 3132 2513 1712 1443 870 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4163 4195 6014 6046 6053 6085 7106 7138 10206 Size: 32 1819 32 7 32 1021 32 3068 Cuts at: 10206 10238 4163 Size: 32 4171 Fragments arranged by size: 4171 3068 1819 1021 32 32 32 32 32 7 Tth111I GACn'n_nGTC Cuts at: 1316 2761 4268 1316 Size: 1445 1507 7294 Fragments arranged by size: 7294 1507 1445 XbaI T'CTAG_A Cuts at: 7582 7582 Size: 10246 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4097 4145 4855 5752 6985 4097 Size: 48 710 897 1233 7358 Fragments arranged by size: 7358 1233 897 710 48 XhoI C'TCGA_G Cuts at: 1943 2555 1943 Size: 612 9634 XmaI C'CCGG_G Cuts at: 1870 2940 6371 6915 7571 1870 Size: 1070 3431 544 656 4545 Fragments arranged by size: 4545 3431 1070 656 544 XmnI GAAnn'nnTTC Cuts at: 3308 5019 8375 3308 Size: 1711 3356 5179 Fragments arranged by size: 5179 3356 1711 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4825 8054 276 Size: 53 83 186 936 3291 3229 2468 Fragments arranged by size: 3291 3229 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BsiWI BstZ17I FseI FspAI NruI PacI PspXI RsrII SfiI SrfI SwaI