(Circular) (Six-base) MAPSORT of: pFN21ASDA1242.seq Check: 4083 from: 1 to: 6208 pFN21ASDA1242 6208 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2651 2651 Size: 6208 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4018 278 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3551 1937 Size: 1614 4594 Acc65I G'GTAC_C Cuts at: 3529 3529 Size: 6208 AclI AA'CG_TT Cuts at: 1563 4335 4708 1563 Size: 2772 373 3063 Fragments arranged by size: 3063 2772 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4271 5655 5923 1632 Size: 2639 1384 268 1917 Fragments arranged by size: 2639 1917 1384 268 AfeI AGC'GCT Cuts at: 2429 2429 Size: 6208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6011 AflIII A'CryG_T Cuts at: 1203 2089 2839 5107 6084 1203 Size: 886 750 2268 977 1327 Fragments arranged by size: 2268 1327 977 886 750 AgeI A'CCGG_T Cuts at: 5039 5039 Size: 6208 AhdI GACnn_n'nnGTC Cuts at: 2608 4937 2608 Size: 2329 3879 AleI CACnn'nnGTG Cuts at: 1166 2645 3202 1166 Size: 1479 557 4172 Fragments arranged by size: 4172 1479 557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2918 1184 Size: 1734 4474 AlwNI CAG_nnn'CTG Cuts at: 2500 3951 5523 2500 Size: 1451 1572 3185 Fragments arranged by size: 3185 1572 1451 ApaLI G'TGCA_C Cuts at: 4265 5421 4265 Size: 1156 5052 ApoI r'AATT_y Cuts at: 1493 3517 3743 3895 6004 1493 Size: 2024 226 152 2109 1697 Fragments arranged by size: 2109 2024 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4762 160 Size: 872 3730 1606 Fragments arranged by size: 3730 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 AvaI C'yCGr_G Cuts at: 1870 1943 2567 3010 3049 3139 3533 1870 Size: 73 624 443 39 90 394 4545 Fragments arranged by size: 4545 624 443 394 90 73 39 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3148 3181 3148 Size: 33 6175 BamHI G'GATC_C Cuts at: 3495 3538 3905 3495 Size: 43 367 5798 Fragments arranged by size: 5798 367 43 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2043 2324 2639 3529 4984 Size: 325 217 627 256 281 315 890 1455 Cuts at: 4984 618 Size: 1842 Fragments arranged by size: 1842 1455 890 627 325 315 281 256 217 BanII G_rGCy'C Cuts at: 1391 2979 3062 3221 3527 1391 Size: 1588 83 159 306 4072 Fragments arranged by size: 4072 1588 306 159 83 BbeI G_GCGC'C Cuts at: 2047 2047 Size: 6208 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5237 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4399 4433 1903 Size: 34 2462 34 3678 Fragments arranged by size: 3678 2462 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3176 4100 5310 3176 Size: 924 1210 4074 Fragments arranged by size: 4074 1210 924 BclI T'GATC_A Cuts at: 1548 1548 Size: 6208 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3098 4819 136 Size: 107 122 71 631 605 1426 1721 1525 Fragments arranged by size: 1721 1525 1426 631 605 122 107 71 BglII A'GATC_T Cuts at: 2532 2784 6203 2532 Size: 252 3419 2537 Fragments arranged by size: 3419 2537 252 BlpI GC'TnA_GC Cuts at: 2624 2865 3625 2624 Size: 241 760 5207 Fragments arranged by size: 5207 760 241 Bme1580I G_kGCm'C Cuts at: 1790 2765 3348 4269 5425 1790 Size: 975 583 921 1156 2573 Fragments arranged by size: 2573 1156 975 921 583 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2126 2229 3939 Size: 416 53 83 399 24 709 103 1710 Cuts at: 3939 4897 442 Size: 958 1753 Fragments arranged by size: 1753 1710 958 709 416 399 103 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2158 2212 2416 3429 4868 1239 Size: 919 54 204 1013 1439 2579 Fragments arranged by size: 2579 1439 1013 919 204 54 Bpu10I CC'TnA_GC Cuts at: 2417 3056 2417 Size: 639 5569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2291 2963 4333 5198 5496 2291 Size: 672 1370 865 298 3003 Fragments arranged by size: 3003 1370 865 672 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2151 3410 4871 882 Size: 617 652 1259 1461 2219 Fragments arranged by size: 2219 1461 1259 652 617 BsaAI yAC'GTr Cuts at: 493 1204 2409 3505 3999 493 Size: 711 1205 1096 494 2702 Fragments arranged by size: 2702 1205 1096 711 494 BsaBI GATnn'nnATC Cuts at: 2063 6202 2063 Size: 4139 2069 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2044 2117 2259 4015 Size: 53 83 186 936 511 73 142 1756 Cuts at: 4015 4397 5032 275 Size: 382 635 1451 Fragments arranged by size: 1756 1451 936 635 511 382 186 142 83 73 53 BsaWI w'CCGG_w Cuts at: 2244 2505 3498 4641 5039 5313 5460 5871 2244 Size: 261 993 1143 398 274 147 411 2581 Fragments arranged by size: 2581 1143 993 411 398 274 261 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2650 2680 3203 3233 3455 Size: 30 165 30 1247 30 523 30 222 Cuts at: 3455 3485 1178 Size: 30 3901 Fragments arranged by size: 3901 1247 523 222 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2993 1186 Size: 1807 4401 BseYI C'CCAG_C Cuts at: 2144 2320 5411 5907 2144 Size: 176 3091 496 2445 Fragments arranged by size: 3091 2445 496 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2363 2801 3086 1163 Size: 573 627 438 285 4285 Fragments arranged by size: 4285 627 573 438 285 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2893 3093 3658 4419 5447 1540 Size: 275 186 892 200 565 761 1028 2301 Fragments arranged by size: 2301 1028 892 761 565 275 200 186 BsiHKAI G_wGCw'C Cuts at: 2625 3084 3272 3480 4269 4354 5425 2625 Size: 459 188 208 789 85 1071 3408 Fragments arranged by size: 3408 1071 789 459 208 188 85 BsmI GAATG_Cn' Cuts at: 2866 3719 3812 2866 Size: 853 93 5262 Fragments arranged by size: 5262 853 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3405 3405 Size: 6208 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2625 2765 2979 3062 3084 3221 3272 Size: 399 835 140 214 83 22 137 51 Cuts at: 3272 3348 3480 3527 4269 4354 5425 1391 Size: 76 132 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 835 742 399 214 140 137 132 85 83 76 51 47 22 BspEI T'CCGG_A Cuts at: 5871 5871 Size: 6208 BspHI T'CATG_A Cuts at: 4097 5827 4097 Size: 1730 4478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2651 3563 844 Size: 1807 912 3489 Fragments arranged by size: 3489 1807 912 BsrBI CCG'CTC Cuts at: 1394 1823 2041 2740 2941 4095 1394 Size: 429 218 699 201 1154 3507 Fragments arranged by size: 3507 1154 699 429 218 201 BsrDI GCAATG_nn' Cuts at: 65 1248 3378 4703 4877 65 Size: 1183 2130 1325 174 1396 Fragments arranged by size: 2130 1396 1325 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2327 2958 4852 5039 1700 Size: 110 517 631 1894 187 2869 Fragments arranged by size: 2869 1894 631 517 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6208 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6208 BssSI C'ACGA_G Cuts at: 4268 5280 4268 Size: 1012 5196 BstAPI GCAn_nnn'nTGC Cuts at: 794 3130 794 Size: 2336 3872 BstBI TT'CG_AA Cuts at: 2007 5012 2007 Size: 3005 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6208 BstXI CCAn_nnnn'nTGG Cuts at: 3262 3262 Size: 6208 BstYI r'GATC_y Cuts at: 1972 2532 2541 2784 3294 3495 3538 3905 4290 Size: 560 9 243 510 201 43 367 385 Cuts at: 4290 4307 5759 5845 5857 6203 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 560 510 385 367 346 243 201 86 43 17 12 9 Bsu36I CC'TnA_GG Cuts at: 3422 3422 Size: 6208 BtgI C'CryG_G Cuts at: 513 1065 2022 2724 513 Size: 552 957 702 3997 Fragments arranged by size: 3997 957 702 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3088 487 Size: 683 56 1862 3607 Fragments arranged by size: 3607 1862 683 56 BtsI GCAGTG_nn' Cuts at: 2927 3197 3209 3724 4518 4538 2927 Size: 270 12 515 794 20 4597 Fragments arranged by size: 4597 794 515 270 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6173 DraI TTT'AAA Cuts at: 3512 3512 Size: 6208 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3463 5215 809 Size: 507 2147 1752 1802 Fragments arranged by size: 2147 1802 1752 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3090 3330 3655 Size: 54 1425 50 275 18 1260 240 325 Cuts at: 3655 4544 6173 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1260 889 325 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3090 3655 1537 Size: 275 1278 565 4090 Fragments arranged by size: 4090 1278 565 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3491 4138 1353 Size: 2138 647 3423 Fragments arranged by size: 3423 2138 647 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2122 4795 5169 5315 387 Size: 1064 138 533 2673 374 146 1280 Fragments arranged by size: 2673 1280 1064 533 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2212 2416 3429 4271 4868 5655 Size: 393 526 54 204 1013 842 597 787 Cuts at: 5655 5923 1239 Size: 268 1524 Fragments arranged by size: 1524 1013 842 787 597 526 393 268 204 54 EcoNI CCTnn'n_nnAGG Cuts at: 3323 3323 Size: 6208 EcoO109I rG'GnC_Cy Cuts at: 2347 2347 Size: 6208 EcoRI G'AATT_C Cuts at: 3517 3895 3517 Size: 378 5830 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2563 2563 Size: 6208 FspI TGC'GCA Cuts at: 4714 4714 Size: 6208 HaeII r_GCGC'y Cuts at: 1678 2047 2431 2852 5355 1678 Size: 369 384 421 2503 2531 Fragments arranged by size: 2531 2503 421 384 369 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 2594 2626 2858 Size: 32 135 32 677 32 313 32 232 Cuts at: 2858 2890 3203 3235 3455 3487 4849 4881 4923 Size: 32 313 32 220 32 1362 32 42 Cuts at: 4923 4955 1373 Size: 32 2626 Fragments arranged by size: 2626 1362 677 313 313 232 220 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3552 3798 669 Size: 988 281 1614 246 3079 Fragments arranged by size: 3079 1614 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6208 HpaI GTT'AAC Cuts at: 3798 3798 Size: 6208 KasI G'GCGC_C Cuts at: 2043 2043 Size: 6208 KpnI G_GTAC'C Cuts at: 3533 3533 Size: 6208 MfeI C'AATT_G Cuts at: 3807 3807 Size: 6208 MluI A'CGCG_T Cuts at: 2089 2839 6084 2089 Size: 750 3245 2213 Fragments arranged by size: 3245 2213 750 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2802 2916 5322 5506 1305 Size: 1497 114 2406 184 2007 Fragments arranged by size: 2406 2007 1497 184 114 MscI TGG'CCA Cuts at: 10 64 1489 1832 3332 10 Size: 54 1425 343 1500 2886 Fragments arranged by size: 2886 1500 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2645 3202 4166 4525 4684 518 Size: 648 933 546 557 964 359 159 2042 Fragments arranged by size: 2042 964 933 648 557 546 359 159 MspA1I CmG'CkG Cuts at: 1606 2872 3155 3162 3624 4301 5449 5694 1606 Size: 1266 283 7 462 677 1148 245 2120 Fragments arranged by size: 2120 1266 1148 677 462 283 245 7 NaeI GCC'GGC Cuts at: 2329 2329 Size: 6208 NarI GG'CG_CC Cuts at: 2044 2044 Size: 6208 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4699 Fragments arranged by size: 4699 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6208 NgoMIV G'CCGG_C Cuts at: 2327 2327 Size: 6208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6208 NotI GC'GGCC_GC Cuts at: 3655 3655 Size: 6208 NspI r_CATG'y Cuts at: 1132 1229 3566 5111 1132 Size: 97 2337 1545 2229 Fragments arranged by size: 2337 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6208 PciI A'CATG_T Cuts at: 5107 5107 Size: 6208 PflMI CCAn_nnn'nTGG Cuts at: 1108 3108 1108 Size: 2000 4208 PfoI T'CCnGG_A Cuts at: 2879 2879 Size: 6208 PmeI GTTT'AAAC Cuts at: 3512 3512 Size: 6208 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4277 4309 5790 5822 4277 Size: 32 1481 32 4663 Fragments arranged by size: 4663 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2347 2347 Size: 6208 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6208 PsiI TTA'TAA Cuts at: 3778 3778 Size: 6208 PspXI vC'TCGA_Gb Cuts at: 3049 3049 Size: 6208 PstI C_TGCA'G Cuts at: 830 2376 2577 2907 2934 2952 3204 3560 830 Size: 1546 201 330 27 18 252 356 3478 Fragments arranged by size: 3478 1546 356 330 252 201 27 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6208 PvuII CAG'CTG Cuts at: 2872 3155 2872 Size: 283 5925 SalI G'TCGA_C Cuts at: 1936 3550 1936 Size: 1614 4594 SbfI CC_TGCA'GG Cuts at: 3560 3560 Size: 6208 ScaI AGT'ACT Cuts at: 4456 6168 4456 Size: 1712 4496 SexAI A'CCwGG_T Cuts at: 2748 2748 Size: 6208 SfcI C'TryA_G Cuts at: 826 1045 2372 2573 2903 2930 2948 3200 3556 Size: 219 1327 201 330 27 18 252 356 Cuts at: 3556 4691 5372 5563 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1327 1135 681 356 330 252 219 201 191 27 18 SfiI GGCCn_nnn'nGGCC Cuts at: 3098 3098 Size: 6208 SfoI GGC'GCC Cuts at: 2045 2045 Size: 6208 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6208 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6208 SmaI CCC'GGG Cuts at: 1872 3535 1872 Size: 1663 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2306 2978 3049 4312 5213 5475 Size: 197 926 363 672 71 1263 901 262 Cuts at: 5475 820 Size: 1553 Fragments arranged by size: 1553 1263 926 901 672 363 262 197 71 SnaBI TAC'GTA Cuts at: 493 3505 3999 493 Size: 3012 494 2702 Fragments arranged by size: 3012 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6208 SphI G_CATG'C Cuts at: 1132 3566 1132 Size: 2434 3774 SspI AAT'ATT Cuts at: 5 52 4132 5 Size: 47 4080 2081 Fragments arranged by size: 4080 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6208 StyI C'CwwG_G Cuts at: 513 1065 2022 2253 3647 513 Size: 552 957 231 1394 3074 Fragments arranged by size: 3074 1394 957 552 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2312 4235 4252 4405 4590 5035 325 Size: 1255 732 1923 17 153 185 445 1498 Fragments arranged by size: 1923 1498 1255 732 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3072 4454 6166 Size: 274 80 33 51 1443 1095 1382 1712 Cuts at: 6166 96 Size: 138 Fragments arranged by size: 1712 1443 1382 1095 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6168 6200 6168 Size: 32 6176 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6208 XbaI T'CTAG_A Cuts at: 3544 3544 Size: 6208 XhoI C'TCGA_G Cuts at: 1943 3049 1943 Size: 1106 5102 XmaI C'CCGG_G Cuts at: 1870 3533 1870 Size: 1663 4545 XmnI GAAnn'nnTTC Cuts at: 4337 4337 Size: 6208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4016 276 Size: 53 83 186 936 2482 2468 Fragments arranged by size: 2482 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SrfI SwaI XcmI