(Circular) (Six-base) MAPSORT of: pFN21ASDA1227.seq Check: 5222 from: 1 to: 7927 pFN21ASDA1227 7927 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5737 278 Size: 53 83 186 936 4201 2468 Fragments arranged by size: 4201 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5270 1937 Size: 3333 4594 Acc65I G'GTAC_C Cuts at: 5248 5248 Size: 7927 AclI AA'CG_TT Cuts at: 1563 6054 6427 1563 Size: 4491 373 3063 Fragments arranged by size: 4491 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4328 4557 4701 4896 5990 7374 7642 1632 Size: 2696 229 144 195 1094 1384 268 1917 Fragments arranged by size: 2696 1917 1384 1094 268 229 195 144 AflII C'TTAA_G Cuts at: 820 1017 3677 820 Size: 197 2660 5070 Fragments arranged by size: 5070 2660 197 AflIII A'CryG_T Cuts at: 1203 2405 2590 3720 4557 4842 4899 6826 7803 Size: 1202 185 1130 837 285 57 1927 977 Cuts at: 7803 1203 Size: 1327 Fragments arranged by size: 1927 1327 1202 1130 977 837 285 185 57 AgeI A'CCGG_T Cuts at: 6758 6758 Size: 7927 AhdI GACnn_n'nnGTC Cuts at: 3801 6656 3801 Size: 2855 5072 AleI CACnn'nnGTG Cuts at: 1166 4473 1166 Size: 3307 4620 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7927 AlwNI CAG_nnn'CTG Cuts at: 3389 3602 4392 5017 5670 7242 3389 Size: 213 790 625 653 1572 4074 Fragments arranged by size: 4074 1572 790 653 625 213 ApaLI G'TGCA_C Cuts at: 5984 7140 5984 Size: 1156 6771 ApoI r'AATT_y Cuts at: 1493 3620 3656 3872 5236 5462 5614 7723 1493 Size: 2127 36 216 1364 226 152 2109 1697 Fragments arranged by size: 2127 2109 1697 1364 226 216 152 36 AseI AT'TA_AT Cuts at: 160 1032 6481 160 Size: 872 5449 1606 Fragments arranged by size: 5449 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7927 AvaI C'yCGr_G Cuts at: 1870 1943 4014 4301 4355 4694 5252 1870 Size: 73 2071 287 54 339 558 4545 Fragments arranged by size: 4545 2071 558 339 287 73 54 BamHI G'GATC_C Cuts at: 5257 5624 5257 Size: 367 7560 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4398 4432 4690 5248 6703 Size: 325 217 627 2611 34 258 558 1455 Cuts at: 6703 618 Size: 1842 Fragments arranged by size: 2611 1842 1455 627 558 325 258 217 34 BanII G_rGCy'C Cuts at: 1391 2152 3321 4518 5246 1391 Size: 761 1169 1197 728 4072 Fragments arranged by size: 4072 1197 1169 761 728 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3695 4604 4626 928 Size: 971 1796 909 22 4229 Fragments arranged by size: 4229 1796 971 909 22 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6118 6152 1903 Size: 34 4181 34 3678 Fragments arranged by size: 4181 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5819 7029 5819 Size: 1210 6717 BclI T'GATC_A Cuts at: 1548 3927 1548 Size: 2379 5548 BfrBI ATG'CAT Cuts at: 4096 4096 Size: 7927 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5037 6538 136 Size: 107 122 71 631 605 3365 1501 1525 Fragments arranged by size: 3365 1525 1501 631 605 122 107 71 BglII A'GATC_T Cuts at: 3681 4010 7922 3681 Size: 329 3912 3686 Fragments arranged by size: 3912 3686 329 BlpI GC'TnA_GC Cuts at: 4209 5344 4209 Size: 1135 6792 Bme1580I G_kGCm'C Cuts at: 1790 2080 4949 5085 5988 7144 1790 Size: 290 2869 136 903 1156 2573 Fragments arranged by size: 2869 2573 1156 903 290 136 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7927 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5658 6616 442 Size: 416 53 83 399 24 4241 958 1753 Fragments arranged by size: 4241 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7927 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3580 3580 Size: 7927 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4814 4900 5039 5043 6587 1239 Size: 3575 86 139 4 1544 2579 Fragments arranged by size: 3575 2579 1544 139 86 4 Bpu10I CC'TnA_GC Cuts at: 4512 4512 Size: 7927 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2373 2702 3204 3550 4027 6052 6917 7215 2373 Size: 329 502 346 477 2025 865 298 3085 Fragments arranged by size: 3085 2025 865 502 477 346 329 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2127 2836 3408 3537 3637 6590 882 Size: 617 628 709 572 129 100 2953 2219 Fragments arranged by size: 2953 2219 709 628 617 572 129 100 BsaAI yAC'GTr Cuts at: 493 1204 4843 5224 5718 493 Size: 711 3639 381 494 2702 Fragments arranged by size: 3639 2702 711 494 381 BsaBI GATnn'nnATC Cuts at: 7921 7921 Size: 7927 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5734 6116 6751 275 Size: 53 83 186 936 4201 382 635 1451 Fragments arranged by size: 4201 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6360 6758 7032 7179 7590 6360 Size: 398 274 147 411 6697 Fragments arranged by size: 6697 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4799 4829 4948 4978 1178 Size: 30 165 30 3396 30 119 30 4127 Fragments arranged by size: 4127 3396 165 119 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 3029 4143 4676 4688 1186 Size: 916 927 1114 533 12 4425 Fragments arranged by size: 4425 1114 927 916 533 12 BseYI C'CCAG_C Cuts at: 3320 3463 4506 7130 7626 3320 Size: 143 1043 2624 496 3621 Fragments arranged by size: 3621 2624 1043 496 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2037 1163 Size: 573 301 7053 Fragments arranged by size: 7053 573 301 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4266 5377 6138 7166 1540 Size: 275 186 2265 1111 761 1028 2301 Fragments arranged by size: 2301 2265 1111 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2152 3528 4820 5988 6073 7144 2152 Size: 1376 1292 1168 85 1071 2935 Fragments arranged by size: 2935 1376 1292 1168 1071 85 BsmI GAATG_Cn' Cuts at: 2686 5438 5531 2686 Size: 2752 93 5082 Fragments arranged by size: 5082 2752 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2099 2099 Size: 7927 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2152 3321 3528 4518 4820 4949 Size: 399 290 72 1169 207 990 302 129 Cuts at: 4949 5085 5246 5988 6073 7144 1391 Size: 136 161 742 85 1071 2174 Fragments arranged by size: 2174 1169 1071 990 742 399 302 290 207 161 136 129 85 72 BspEI T'CCGG_A Cuts at: 7590 7590 Size: 7927 BspHI T'CATG_A Cuts at: 5816 7546 5816 Size: 1730 6197 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3689 4358 5282 844 Size: 2845 669 924 3489 Fragments arranged by size: 3489 2845 924 669 BsrBI CCG'CTC Cuts at: 1394 1823 4246 5814 1394 Size: 429 2423 1568 3507 Fragments arranged by size: 3507 2423 1568 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2559 3670 6422 6596 65 Size: 1183 1311 1111 2752 174 1396 Fragments arranged by size: 2752 1396 1311 1183 1111 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6571 6758 1700 Size: 110 4761 187 2869 Fragments arranged by size: 4761 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7927 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7927 BssSI C'ACGA_G Cuts at: 4241 4319 5987 6999 4241 Size: 78 1668 1012 5169 Fragments arranged by size: 5169 1668 1012 78 BstAPI GCAn_nnn'nTGC Cuts at: 794 3672 794 Size: 2878 5049 BstBI TT'CG_AA Cuts at: 2007 6731 2007 Size: 4724 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7927 BstYI r'GATC_y Cuts at: 1972 2693 2984 3681 4010 5007 5257 5624 6009 Size: 721 291 697 329 997 250 367 385 Cuts at: 6009 6026 7478 7564 7576 7922 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 997 721 697 385 367 346 329 291 250 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3242 4286 3242 Size: 1044 6883 BtgI C'CryG_G Cuts at: 513 1065 2022 2846 513 Size: 552 957 824 5594 Fragments arranged by size: 5594 957 824 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3221 487 Size: 683 56 1995 5193 Fragments arranged by size: 5193 1995 683 56 BtsI GCAGTG_nn' Cuts at: 2745 4997 5443 6237 6257 2745 Size: 2252 446 794 20 4415 Fragments arranged by size: 4415 2252 794 446 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7927 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2841 2876 529 Size: 35 2277 35 5580 Fragments arranged by size: 5580 2277 35 35 DraI TTT'AAA Cuts at: 2274 3180 3661 5140 5231 2274 Size: 906 481 1479 91 4970 Fragments arranged by size: 4970 1479 906 481 91 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4881 6934 809 Size: 507 3565 2053 1802 Fragments arranged by size: 3565 2053 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2645 2849 4263 Size: 54 1425 50 275 18 815 204 1414 Cuts at: 4263 5374 6263 7892 8 Size: 1111 889 1629 43 Fragments arranged by size: 1629 1425 1414 1111 889 815 275 204 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4263 5374 1537 Size: 275 2451 1111 4090 Fragments arranged by size: 4090 2451 1111 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2303 2834 4222 4680 5857 1353 Size: 950 531 1388 458 1177 3423 Fragments arranged by size: 3423 1388 1177 950 531 458 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6514 6888 7034 387 Size: 1064 138 4925 374 146 1280 Fragments arranged by size: 4925 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2150 2150 Size: 7927 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4328 4557 4701 4814 4896 4900 5039 Size: 393 2696 229 144 113 82 4 139 Cuts at: 5039 5043 5990 6587 7374 7642 1239 Size: 4 947 597 787 268 1524 Fragments arranged by size: 2696 1524 947 787 597 393 268 229 144 139 113 82 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2084 Size: 7927 EcoO109I rG'GnC_Cy Cuts at: 3246 3922 4497 4724 3246 Size: 676 575 227 6449 Fragments arranged by size: 6449 676 575 227 EcoRI G'AATT_C Cuts at: 5236 5614 5236 Size: 378 7549 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2556 2980 4021 2556 Size: 424 1041 6462 Fragments arranged by size: 6462 1041 424 FspI TGC'GCA Cuts at: 3305 4367 6433 3305 Size: 1062 2066 4799 Fragments arranged by size: 4799 2066 1062 FspAI rTGC'GCAy Cuts at: 3305 4367 3305 Size: 1062 6865 HaeII r_GCGC'y Cuts at: 1678 7074 1678 Size: 5396 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2875 2907 3086 3118 3515 Size: 32 135 32 1303 32 179 32 397 Cuts at: 3515 3547 3787 3819 4999 5031 6568 6600 6642 Size: 32 240 32 1180 32 1537 32 42 Cuts at: 6642 6674 1373 Size: 32 2626 Fragments arranged by size: 2626 1537 1303 1180 397 240 179 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3718 4585 5271 5517 669 Size: 988 281 1329 451 867 686 246 3079 Fragments arranged by size: 3079 1329 988 867 686 451 281 246 HindIII A'AGCT_T Cuts at: 748 4854 748 Size: 4106 3821 HpaI GTT'AAC Cuts at: 3718 4585 5517 3718 Size: 867 932 6128 Fragments arranged by size: 6128 932 867 KpnI G_GTAC'C Cuts at: 5252 5252 Size: 7927 MfeI C'AATT_G Cuts at: 5526 5526 Size: 7927 MluI A'CGCG_T Cuts at: 7803 7803 Size: 7927 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2110 2426 3315 3612 7041 7225 1305 Size: 805 316 889 297 3429 184 2007 Fragments arranged by size: 3429 2007 889 805 316 297 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6105 Fragments arranged by size: 6105 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3711 4473 5885 6244 6403 518 Size: 648 2545 762 1412 359 159 2042 Fragments arranged by size: 2545 2042 1412 762 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 3331 3389 4792 5343 6020 7168 7413 Size: 501 1224 58 1403 551 677 1148 245 Cuts at: 7413 1606 Size: 2120 Fragments arranged by size: 2120 1403 1224 1148 677 551 501 245 58 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6418 Fragments arranged by size: 6418 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7927 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7927 NotI GC'GGCC_GC Cuts at: 5374 5374 Size: 7927 NsiI A_TGCA'T Cuts at: 4098 4098 Size: 7927 NspI r_CATG'y Cuts at: 1132 1229 2594 3724 3857 4151 4366 4903 5285 Size: 97 1365 1130 133 294 215 537 382 Cuts at: 5285 6830 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1365 1130 537 382 294 215 133 97 PasI CC'CwG_GG Cuts at: 1348 3109 1348 Size: 1761 6166 PciI A'CATG_T Cuts at: 2590 3720 4899 6826 2590 Size: 1130 1179 1927 3691 Fragments arranged by size: 3691 1927 1179 1130 PflMI CCAn_nnn'nTGG Cuts at: 1108 2981 4746 5017 1108 Size: 1873 1765 271 4018 Fragments arranged by size: 4018 1873 1765 271 PfoI T'CCnGG_A Cuts at: 4099 4099 Size: 7927 PmeI GTTT'AAAC Cuts at: 5231 5231 Size: 7927 PmlI CAC'GTG Cuts at: 4843 4843 Size: 7927 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5996 6028 7509 7541 5996 Size: 32 1481 32 6382 Fragments arranged by size: 6382 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3246 4724 3246 Size: 1478 6449 PshAI GACnn'nnGTC Cuts at: 1613 3759 4626 1613 Size: 2146 867 4914 Fragments arranged by size: 4914 2146 867 PsiI TTA'TAA Cuts at: 5497 5497 Size: 7927 PspXI vC'TCGA_Gb Cuts at: 4355 4355 Size: 7927 PstI C_TGCA'G Cuts at: 830 4283 5279 830 Size: 3453 996 3478 Fragments arranged by size: 3478 3453 996 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7927 PvuII CAG'CTG Cuts at: 2107 3389 4792 2107 Size: 1282 1403 5242 Fragments arranged by size: 5242 1403 1282 RsrII CG'GwC_CG Cuts at: 3487 3487 Size: 7927 SacI G_AGCT'C Cuts at: 2152 2152 Size: 7927 SalI G'TCGA_C Cuts at: 1936 5269 1936 Size: 3333 4594 SapI GCTCTTCn'nnn_ Cuts at: 2303 2303 Size: 7927 SbfI CC_TGCA'GG Cuts at: 5279 5279 Size: 7927 ScaI AGT'ACT Cuts at: 6175 7887 6175 Size: 1712 6215 SexAI A'CCwGG_T Cuts at: 2788 4401 2788 Size: 1613 6314 SfcI C'TryA_G Cuts at: 826 1045 2341 4279 5275 6410 7091 7282 826 Size: 219 1296 1938 996 1135 681 191 1471 Fragments arranged by size: 1938 1471 1296 1135 996 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7927 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7927 SmaI CCC'GGG Cuts at: 1872 5254 1872 Size: 3382 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2388 2681 3219 3565 3677 4006 Size: 197 926 445 293 538 346 112 329 Cuts at: 4006 4014 4355 6031 6932 7194 820 Size: 8 341 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 538 445 346 341 329 293 262 197 112 8 SnaBI TAC'GTA Cuts at: 493 5224 5718 493 Size: 4731 494 2702 Fragments arranged by size: 4731 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7927 SphI G_CATG'C Cuts at: 1132 4151 4366 5285 1132 Size: 3019 215 919 3774 Fragments arranged by size: 3774 3019 919 215 SspI AAT'ATT Cuts at: 5 52 5851 5 Size: 47 5799 2081 Fragments arranged by size: 5799 2081 47 StuI AGG'CCT Cuts at: 1517 3217 5040 1517 Size: 1700 1823 4404 Fragments arranged by size: 4404 1823 1700 StyI C'CwwG_G Cuts at: 513 1065 2022 4114 5366 513 Size: 552 957 2092 1252 3074 Fragments arranged by size: 3074 2092 1252 957 552 SwaI ATTT'AAAT Cuts at: 3661 3661 Size: 7927 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3394 3504 4450 5954 5971 6124 6309 Size: 1255 1814 110 946 1504 17 153 185 Cuts at: 6309 6754 325 Size: 445 1498 Fragments arranged by size: 1814 1504 1498 1255 946 445 185 153 110 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2946 4037 6173 Size: 274 80 33 51 1443 969 1091 2136 Cuts at: 6173 7885 96 Size: 1712 138 Fragments arranged by size: 2136 1712 1443 1091 969 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4799 4831 4948 4980 7887 7919 4799 Size: 32 117 32 2907 32 4807 Fragments arranged by size: 4807 2907 117 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7927 XbaI T'CTAG_A Cuts at: 2824 5263 2824 Size: 2439 5488 XhoI C'TCGA_G Cuts at: 1943 4014 4355 1943 Size: 2071 341 5515 Fragments arranged by size: 5515 2071 341 XmaI C'CCGG_G Cuts at: 1870 5252 1870 Size: 3382 4545 XmnI GAAnn'nnTTC Cuts at: 2184 2613 4772 6056 2184 Size: 429 2159 1284 4055 Fragments arranged by size: 4055 2159 1284 429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5735 276 Size: 53 83 186 936 4201 2468 Fragments arranged by size: 4201 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstXI BstZ17I DraIII EcoRV FseI KasI NaeI NarI NgoMIV NruI PacI PspOMI PsrI SacII SanDI SfiI SfoI SrfI XcmI