(Circular) (Six-base) MAPSORT of: pFN21ASDA1225.seq Check: 3114 from: 1 to: 8842 pFN21ASDA1225 8842 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6652 278 Size: 53 83 186 936 5116 2468 Fragments arranged by size: 5116 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2071 6185 1937 Size: 134 4114 4594 Fragments arranged by size: 4594 4114 134 Acc65I G'GTAC_C Cuts at: 2056 6163 2056 Size: 4107 4735 AclI AA'CG_TT Cuts at: 1563 2270 6969 7342 1563 Size: 707 4699 373 3063 Fragments arranged by size: 4699 3063 707 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2643 2801 3735 3837 4602 5343 6003 6905 Size: 1011 158 934 102 765 741 660 902 Cuts at: 6905 8289 8557 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1011 934 902 765 741 660 268 158 102 AflII C'TTAA_G Cuts at: 820 1017 2992 3494 820 Size: 197 1975 502 6168 Fragments arranged by size: 6168 1975 502 197 AflIII A'CryG_T Cuts at: 1203 2774 3297 7741 8718 1203 Size: 1571 523 4444 977 1327 Fragments arranged by size: 4444 1571 1327 977 523 AgeI A'CCGG_T Cuts at: 7673 7673 Size: 8842 AhdI GACnn_n'nnGTC Cuts at: 7571 7571 Size: 8842 AleI CACnn'nnGTG Cuts at: 1166 2615 1166 Size: 1449 7393 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8842 AlwNI CAG_nnn'CTG Cuts at: 3749 5276 6585 8157 3749 Size: 1527 1309 1572 4434 Fragments arranged by size: 4434 1572 1527 1309 ApaLI G'TGCA_C Cuts at: 6899 8055 6899 Size: 1156 7686 ApoI r'AATT_y Cuts at: 1493 2488 2606 2766 3166 3457 4123 5107 6151 Size: 995 118 160 400 291 666 984 1044 Cuts at: 6151 6377 6529 8638 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1044 995 984 666 400 291 226 160 152 118 AseI AT'TA_AT Cuts at: 160 1032 2298 3141 3869 4095 4416 5469 7396 Size: 872 1266 843 728 226 321 1053 1927 Cuts at: 7396 160 Size: 1606 Fragments arranged by size: 1927 1606 1266 1053 872 843 728 321 226 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8842 AvaI C'yCGr_G Cuts at: 1870 1943 6167 1870 Size: 73 4224 4545 Fragments arranged by size: 4545 4224 73 BamHI G'GATC_C Cuts at: 5884 6172 6539 5884 Size: 288 367 8187 Fragments arranged by size: 8187 367 288 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 3439 6163 7618 618 Size: 325 217 627 269 1383 2724 1455 1842 Fragments arranged by size: 2724 1842 1455 1383 627 325 269 217 BanII G_rGCy'C Cuts at: 1391 3397 5314 6161 1391 Size: 2006 1917 847 4072 Fragments arranged by size: 4072 2006 1917 847 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3426 3564 3629 928 Size: 971 1527 138 65 6141 Fragments arranged by size: 6141 1527 971 138 65 BbvCI CC'TCA_GC Cuts at: 4698 5043 4698 Size: 345 8497 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7033 7067 1903 Size: 34 5096 34 3678 Fragments arranged by size: 5096 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4470 6734 7944 4470 Size: 2264 1210 5368 Fragments arranged by size: 5368 2264 1210 BclI T'GATC_A Cuts at: 1548 3010 4783 1548 Size: 1462 1773 5607 Fragments arranged by size: 5607 1773 1462 BfrBI ATG'CAT Cuts at: 5438 5438 Size: 8842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5692 7453 136 Size: 107 122 71 631 605 4020 1761 1525 Fragments arranged by size: 4020 1761 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4585 8837 4585 Size: 4252 4590 BlpI GC'TnA_GC Cuts at: 6259 6259 Size: 8842 Bme1580I G_kGCm'C Cuts at: 1790 6903 8059 1790 Size: 5113 1156 2573 Fragments arranged by size: 5113 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2440 4507 6573 Size: 416 53 83 399 24 1023 2067 2066 Cuts at: 6573 7531 442 Size: 958 1753 Fragments arranged by size: 2067 2066 1753 1023 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8842 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5371 5371 Size: 8842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4053 5565 5629 7502 1239 Size: 2814 1512 64 1873 2579 Fragments arranged by size: 2814 2579 1873 1512 64 Bpu10I CC'TnA_GC Cuts at: 4698 5043 4698 Size: 345 8497 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2492 2651 3113 3381 6967 7832 8130 2492 Size: 159 462 268 3586 865 298 3204 Fragments arranged by size: 3586 3204 865 462 298 268 159 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2081 3263 3542 5389 7505 882 Size: 617 582 1182 279 1847 2116 2219 Fragments arranged by size: 2219 2116 1847 1182 617 582 279 BsaAI yAC'GTr Cuts at: 493 1204 6139 6633 493 Size: 711 4935 494 2702 Fragments arranged by size: 4935 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2187 4866 8836 2187 Size: 2679 3970 2193 Fragments arranged by size: 3970 2679 2193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6649 7031 7666 275 Size: 53 83 186 936 5116 382 635 1451 Fragments arranged by size: 5116 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7275 7673 7947 8094 8505 7275 Size: 398 274 147 411 7612 Fragments arranged by size: 7612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8617 Fragments arranged by size: 8617 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 5031 5361 1186 Size: 913 2932 330 4667 Fragments arranged by size: 4667 2932 913 330 BseYI C'CCAG_C Cuts at: 2574 5170 8045 8541 2574 Size: 2596 2875 496 2875 Fragments arranged by size: 2875 2875 2596 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4908 5391 1163 Size: 573 3172 483 4614 Fragments arranged by size: 4614 3172 573 483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5212 6292 7053 8081 1540 Size: 275 186 3211 1080 761 1028 2301 Fragments arranged by size: 3211 2301 1080 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4440 5069 5458 6903 6988 8059 4440 Size: 629 389 1445 85 1071 5223 Fragments arranged by size: 5223 1445 1071 629 389 85 BsmI GAATG_Cn' Cuts at: 5563 6353 6446 5563 Size: 790 93 7959 Fragments arranged by size: 7959 790 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3397 4440 5069 5314 5458 6161 6903 Size: 399 1607 1043 629 245 144 703 742 Cuts at: 6903 6988 8059 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1607 1071 1043 742 703 629 399 245 144 85 BspEI T'CCGG_A Cuts at: 8505 8505 Size: 8842 BspHI T'CATG_A Cuts at: 4189 6731 8461 4189 Size: 2542 1730 4570 Fragments arranged by size: 4570 2542 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4786 6197 844 Size: 3942 1411 3489 Fragments arranged by size: 3942 3489 1411 BsrBI CCG'CTC Cuts at: 1394 1823 6729 1394 Size: 429 4906 3507 Fragments arranged by size: 4906 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2113 2283 4790 7337 7511 65 Size: 1183 865 170 2507 2547 174 1396 Fragments arranged by size: 2547 2507 1396 1183 865 174 170 BsrFI r'CCGG_y Cuts at: 1700 1810 7486 7673 1700 Size: 110 5676 187 2869 Fragments arranged by size: 5676 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3526 3694 96 Size: 3430 168 5244 Fragments arranged by size: 5244 3430 168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8842 BssSI C'ACGA_G Cuts at: 5601 6902 7914 5601 Size: 1301 1012 6529 Fragments arranged by size: 6529 1301 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2285 794 Size: 1491 7351 BstBI TT'CG_AA Cuts at: 2007 7646 2007 Size: 5639 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8842 BstYI r'GATC_y Cuts at: 1972 4585 4616 5575 5884 6172 6539 6924 6941 Size: 2613 31 959 309 288 367 385 17 Cuts at: 6941 8393 8479 8491 8837 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2613 1977 1452 959 385 367 346 309 288 86 31 17 12 Bsu36I CC'TnA_GG Cuts at: 5697 5730 5697 Size: 33 8809 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 8290 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8103 Fragments arranged by size: 8103 683 56 BtsI GCAGTG_nn' Cuts at: 4978 6078 6358 7152 7172 4978 Size: 1100 280 794 20 6648 Fragments arranged by size: 6648 1100 794 280 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8807 DraI TTT'AAA Cuts at: 3462 4158 6146 3462 Size: 696 1988 6158 Fragments arranged by size: 6158 1988 696 DraIII CAC_nnn'GTG Cuts at: 4995 4995 Size: 8842 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7849 809 Size: 507 6533 1802 Fragments arranged by size: 6533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6289 7178 8807 Size: 54 1425 50 275 18 4459 889 1629 Cuts at: 8807 8 Size: 43 Fragments arranged by size: 4459 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6289 1537 Size: 275 4477 4090 Fragments arranged by size: 4477 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2193 3252 3540 3562 3831 4230 4875 Size: 723 117 1059 288 22 269 399 645 Cuts at: 4875 6772 1353 Size: 1897 3423 Fragments arranged by size: 3423 1897 1059 723 645 399 288 269 117 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3866 7429 7803 7949 387 Size: 1064 138 2277 3563 374 146 1280 Fragments arranged by size: 3563 2277 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2643 2801 3735 3837 4053 4602 5343 Size: 393 1011 158 934 102 216 549 741 Cuts at: 5343 5565 5629 6003 6905 7502 8289 8557 1239 Size: 222 64 374 902 597 787 268 1524 Fragments arranged by size: 1524 1011 934 902 787 741 597 549 393 374 268 222 216 158 102 64 EcoNI CCTnn'n_nnAGG Cuts at: 5835 5835 Size: 8842 EcoO109I rG'GnC_Cy Cuts at: 4901 4901 Size: 8842 EcoRI G'AATT_C Cuts at: 2606 6151 6529 2606 Size: 3545 378 4919 Fragments arranged by size: 4919 3545 378 EcoRV GAT'ATC Cuts at: 3470 3470 Size: 8842 FspI TGC'GCA Cuts at: 7348 7348 Size: 8842 HaeII r_GCGC'y Cuts at: 1678 7989 1678 Size: 6311 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3212 3244 3935 3967 4817 Size: 32 135 32 1640 32 691 32 850 Cuts at: 4817 4849 5282 5314 5321 5353 7483 7515 7557 Size: 32 433 32 7 32 2130 32 42 Cuts at: 7557 7589 1373 Size: 32 2626 Fragments arranged by size: 2626 2130 1640 850 691 433 135 42 32 32 32 32 32 32 32 32 32 7 HincII GTy'rAC Cuts at: 669 1657 1938 3790 4267 4558 6186 6432 669 Size: 988 281 1852 477 291 1628 246 3079 Fragments arranged by size: 3079 1852 1628 988 477 291 281 246 HindIII A'AGCT_T Cuts at: 748 2958 4402 748 Size: 2210 1444 5188 Fragments arranged by size: 5188 2210 1444 HpaI GTT'AAC Cuts at: 3790 6432 3790 Size: 2642 6200 KpnI G_GTAC'C Cuts at: 2060 6167 2060 Size: 4107 4735 MfeI C'AATT_G Cuts at: 2376 3197 3991 6441 2376 Size: 821 794 2450 4777 Fragments arranged by size: 4777 2450 821 794 MluI A'CGCG_T Cuts at: 8718 8718 Size: 8842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3497 4594 7956 8140 1305 Size: 2192 1097 3362 184 2007 Fragments arranged by size: 3362 2192 2007 1097 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7020 Fragments arranged by size: 7020 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2615 3409 3798 4542 5124 6800 7159 Size: 648 1449 794 389 744 582 1676 359 Cuts at: 7159 7318 518 Size: 159 2042 Fragments arranged by size: 2042 1676 1449 794 744 648 582 389 359 159 MspA1I CmG'CkG Cuts at: 1606 2431 2578 3391 4283 5360 6258 6935 8083 Size: 825 147 813 892 1077 898 677 1148 Cuts at: 8083 8328 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1077 898 892 825 813 677 245 147 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8290 NdeI CA'TA_TG Cuts at: 387 387 Size: 8842 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8842 NotI GC'GGCC_GC Cuts at: 6289 6289 Size: 8842 NsiI A_TGCA'T Cuts at: 5440 5440 Size: 8842 NspI r_CATG'y Cuts at: 1132 1229 2778 3301 6200 7745 1132 Size: 97 1549 523 2899 1545 2229 Fragments arranged by size: 2899 2229 1549 1545 523 97 PasI CC'CwG_GG Cuts at: 1348 3447 1348 Size: 2099 6743 PciI A'CATG_T Cuts at: 2774 3297 7741 2774 Size: 523 4444 3875 Fragments arranged by size: 4444 3875 523 PflMI CCAn_nnn'nTGG Cuts at: 1108 3044 3280 5848 5894 1108 Size: 1936 236 2568 46 4056 Fragments arranged by size: 4056 2568 1936 236 46 PfoI T'CCnGG_A Cuts at: 5581 5581 Size: 8842 PmeI GTTT'AAAC Cuts at: 6146 6146 Size: 8842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 6911 6943 8424 8456 4163 Size: 32 2716 32 1481 32 4549 Fragments arranged by size: 4549 2716 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8842 PsiI TTA'TAA Cuts at: 4334 6050 6412 4334 Size: 1716 362 6764 Fragments arranged by size: 6764 1716 362 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2011 2043 2011 Size: 32 8810 PstI C_TGCA'G Cuts at: 830 4981 5227 6009 6194 830 Size: 4151 246 782 185 3478 Fragments arranged by size: 4151 3478 782 246 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8842 PvuII CAG'CTG Cuts at: 2431 2578 4283 2431 Size: 147 1705 6990 Fragments arranged by size: 6990 1705 147 SalI G'TCGA_C Cuts at: 1936 6184 1936 Size: 4248 4594 SapI GCTCTTCn'nnn_ Cuts at: 2193 4230 4875 2193 Size: 2037 645 6160 Fragments arranged by size: 6160 2037 645 SbfI CC_TGCA'GG Cuts at: 6194 6194 Size: 8842 ScaI AGT'ACT Cuts at: 2628 3637 5622 7090 8802 2628 Size: 1009 1985 1468 1712 2668 Fragments arranged by size: 2668 1985 1712 1468 1009 SfcI C'TryA_G Cuts at: 826 1045 2904 3191 3642 4977 5223 6005 6040 Size: 219 1859 287 451 1335 246 782 35 Cuts at: 6040 6190 7325 8006 8197 826 Size: 150 1135 681 191 1471 Fragments arranged by size: 1859 1471 1335 1135 782 681 451 287 246 219 191 150 35 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8842 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8842 SmaI CCC'GGG Cuts at: 1872 6169 1872 Size: 4297 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2471 2630 2992 3092 3396 3494 Size: 197 926 528 159 362 100 304 98 Cuts at: 3494 6946 7847 8109 820 Size: 3452 901 262 1553 Fragments arranged by size: 3452 1553 926 901 528 362 304 262 197 159 100 98 SnaBI TAC'GTA Cuts at: 493 6139 6633 493 Size: 5646 494 2702 Fragments arranged by size: 5646 2702 494 SpeI A'CTAG_T Cuts at: 152 5522 152 Size: 5370 3472 SphI G_CATG'C Cuts at: 1132 6200 1132 Size: 5068 3774 SspI AAT'ATT Cuts at: 5 52 2517 2746 6055 6766 5 Size: 47 2465 229 3309 711 2081 Fragments arranged by size: 3309 2465 2081 711 229 47 StuI AGG'CCT Cuts at: 1517 5695 1517 Size: 4178 4664 StyI C'CwwG_G Cuts at: 513 1065 2163 3314 6281 513 Size: 552 1098 1151 2967 3074 Fragments arranged by size: 3074 2967 1151 1098 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3104 3605 5226 6869 6886 7039 7224 Size: 1255 1524 501 1621 1643 17 153 185 Cuts at: 7224 7669 325 Size: 445 1498 Fragments arranged by size: 1643 1621 1524 1498 1255 501 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2626 3526 3635 Size: 274 80 33 51 1443 649 900 109 Cuts at: 3635 3694 4709 5237 5620 7088 8800 96 Size: 59 1015 528 383 1468 1712 138 Fragments arranged by size: 1712 1468 1443 1015 900 649 528 383 274 138 109 80 59 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4608 4640 6057 6089 8802 8834 4608 Size: 32 1417 32 2713 32 4616 Fragments arranged by size: 4616 2713 1417 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8842 XbaI T'CTAG_A Cuts at: 5756 6178 5756 Size: 422 8420 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8842 XmaI C'CCGG_G Cuts at: 1870 6167 1870 Size: 4297 4545 XmnI GAAnn'nnTTC Cuts at: 2130 2202 2784 6971 2130 Size: 72 582 4187 4001 Fragments arranged by size: 4187 4001 582 72 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6650 276 Size: 53 83 186 936 5116 2468 Fragments arranged by size: 5116 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI