(Circular) (Six-base) MAPSORT of: pFN21ASDA1202.seq Check: 8681 from: 1 to: 9223 pFN21ASDA1202 9223 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3291 3668 3291 Size: 377 8846 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7033 278 Size: 53 83 186 936 5497 2468 Fragments arranged by size: 5497 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6493 6566 1937 Size: 4556 73 4594 Fragments arranged by size: 4594 4556 73 Acc65I G'GTAC_C Cuts at: 6544 6544 Size: 9223 AclI AA'CG_TT Cuts at: 1563 7350 7723 1563 Size: 5787 373 3063 Fragments arranged by size: 5787 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2376 2377 2406 2855 4556 5118 5811 6329 Size: 744 1 29 449 1701 562 693 518 Cuts at: 6329 7286 8670 8938 1632 Size: 957 1384 268 1917 Fragments arranged by size: 1917 1701 1384 957 744 693 562 518 449 268 29 1 AflII C'TTAA_G Cuts at: 820 1017 2096 820 Size: 197 1079 7947 Fragments arranged by size: 7947 1079 197 AflIII A'CryG_T Cuts at: 1203 2430 2562 8122 9099 1203 Size: 1227 132 5560 977 1327 Fragments arranged by size: 5560 1327 1227 977 132 AgeI A'CCGG_T Cuts at: 8054 8054 Size: 9223 AhdI GACnn_n'nnGTC Cuts at: 7952 7952 Size: 9223 AleI CACnn'nnGTG Cuts at: 1166 3859 4653 1166 Size: 2693 794 5736 Fragments arranged by size: 5736 2693 794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3621 1184 Size: 2437 6786 AlwNI CAG_nnn'CTG Cuts at: 3271 4157 4677 6277 6966 8538 3271 Size: 886 520 1600 689 1572 3956 Fragments arranged by size: 3956 1600 1572 886 689 520 ApaI G_GGCC'C Cuts at: 2820 2840 5836 6507 2820 Size: 20 2996 671 5536 Fragments arranged by size: 5536 2996 671 20 ApaLI G'TGCA_C Cuts at: 3293 7280 8436 3293 Size: 3987 1156 4080 Fragments arranged by size: 4080 3987 1156 ApoI r'AATT_y Cuts at: 1493 3807 4624 4919 6119 6532 6758 6910 9019 Size: 2314 817 295 1200 413 226 152 2109 Cuts at: 9019 1493 Size: 1697 Fragments arranged by size: 2314 2109 1697 1200 817 413 295 226 152 AseI AT'TA_AT Cuts at: 160 1032 7777 160 Size: 872 6745 1606 Fragments arranged by size: 6745 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9223 AvaI C'yCGr_G Cuts at: 1870 1943 3744 4859 5232 5617 6198 6458 6548 Size: 73 1801 1115 373 385 581 260 90 Cuts at: 6548 1870 Size: 4545 Fragments arranged by size: 4545 1801 1115 581 385 373 260 90 73 AvrII C'CTAG_G Cuts at: 5938 5938 Size: 9223 BamHI G'GATC_C Cuts at: 3125 6553 6920 3125 Size: 3428 367 5428 Fragments arranged by size: 5428 3428 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 3348 4488 4797 4876 Size: 325 217 627 290 1271 1140 309 79 Cuts at: 4876 5218 6544 7999 618 Size: 342 1326 1455 1842 Fragments arranged by size: 1842 1455 1326 1271 1140 627 342 325 309 290 217 79 BanII G_rGCy'C Cuts at: 1391 2820 2840 2887 3086 3330 3702 3900 4469 Size: 1429 20 47 199 244 372 198 569 Cuts at: 4469 4708 5156 5262 5618 5836 6507 6542 1391 Size: 239 448 106 356 218 671 35 4072 Fragments arranged by size: 4072 1429 671 569 448 372 356 244 239 218 199 198 106 47 35 20 BbeI G_GCGC'C Cuts at: 4492 5222 4492 Size: 730 8493 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4005 4967 928 Size: 971 2106 962 5184 Fragments arranged by size: 5184 2106 971 962 BbvCI CC'TCA_GC Cuts at: 4025 4845 5594 4025 Size: 820 749 7654 Fragments arranged by size: 7654 820 749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7414 7448 1903 Size: 34 5477 34 3678 Fragments arranged by size: 5477 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2798 7115 8325 2798 Size: 4317 1210 3696 Fragments arranged by size: 4317 3696 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9223 BfrBI ATG'CAT Cuts at: 2374 4815 2374 Size: 2441 6782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3081 7834 136 Size: 107 122 71 631 605 1409 4753 1525 Fragments arranged by size: 4753 1525 1409 631 605 122 107 71 BglII A'GATC_T Cuts at: 9218 9218 Size: 9223 BlpI GC'TnA_GC Cuts at: 5727 6640 5727 Size: 913 8310 Bme1580I G_kGCm'C Cuts at: 1790 2080 2632 2820 2840 3259 3297 3351 4858 Size: 290 552 188 20 419 38 54 1507 Cuts at: 4858 5836 6507 7284 8440 1790 Size: 978 671 777 1156 2573 Fragments arranged by size: 2573 1507 1156 978 777 671 552 419 290 188 54 38 20 BmgBI CAC'GTC Cuts at: 1318 2431 1318 Size: 1113 8110 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2697 5278 5356 Size: 416 53 83 399 24 1280 2581 78 Cuts at: 5356 5515 6954 7912 442 Size: 159 1439 958 1753 Fragments arranged by size: 2581 1753 1439 1280 958 416 399 159 83 78 53 24 BmtI G_CTAG'C Cuts at: 1056 3197 1056 Size: 2141 7082 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2674 2906 3995 4224 5170 2674 Size: 232 1089 229 946 6727 Fragments arranged by size: 6727 1089 946 232 229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2363 2528 2835 2863 3815 4194 4453 5630 Size: 1124 165 307 28 952 379 259 1177 Cuts at: 5630 6461 7883 1239 Size: 831 1422 2579 Fragments arranged by size: 2579 1422 1177 1124 952 831 379 307 259 165 28 Bpu10I CC'TnA_GC Cuts at: 3198 4025 4845 5594 5718 3198 Size: 827 820 749 124 6703 Fragments arranged by size: 6703 827 820 749 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 4284 5057 7348 8213 8511 2258 Size: 2026 773 2291 865 298 2970 Fragments arranged by size: 2970 2291 2026 865 773 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2720 3881 4212 4423 5273 6358 7886 Size: 617 1221 1161 331 211 850 1085 1528 Cuts at: 7886 882 Size: 2219 Fragments arranged by size: 2219 1528 1221 1161 1085 850 617 331 211 BsaAI yAC'GTr Cuts at: 493 1204 6319 6520 7014 493 Size: 711 5115 201 494 2702 Fragments arranged by size: 5115 2702 711 494 201 BsaBI GATnn'nnATC Cuts at: 9217 9217 Size: 9223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4489 5219 7030 7412 Size: 53 83 186 936 2956 730 1811 382 Cuts at: 7412 8047 275 Size: 635 1451 Fragments arranged by size: 2956 1811 1451 936 730 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2265 7656 8054 8328 8475 8886 2265 Size: 5391 398 274 147 411 2602 Fragments arranged by size: 5391 2602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4130 4160 4695 4725 1178 Size: 30 165 30 2727 30 535 30 5676 Fragments arranged by size: 5676 2727 535 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2305 2663 2882 3829 4138 5159 5313 5316 Size: 1119 358 219 947 309 1021 154 3 Cuts at: 5316 5325 5328 5444 5447 5450 5453 5456 5459 Size: 9 3 116 3 3 3 3 3 Cuts at: 5459 5492 5495 5498 5501 5510 5513 5516 5519 Size: 33 3 3 3 9 3 3 3 Cuts at: 5519 5522 5582 5960 6092 6458 1186 Size: 3 60 378 132 366 3951 Fragments arranged by size: 3951 1119 1021 947 378 366 358 309 219 154 132 116 60 33 9 9 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2771 3825 3962 4304 5044 5376 5641 6030 6506 Size: 1054 137 342 740 332 265 389 476 Cuts at: 6506 8426 8922 2771 Size: 1920 496 3072 Fragments arranged by size: 3072 1920 1054 740 496 476 389 342 332 265 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2459 3730 4934 5391 1163 Size: 573 345 378 1271 1204 457 4995 Fragments arranged by size: 4995 1271 1204 573 457 378 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6673 7434 8462 1540 Size: 275 186 4672 761 1028 2301 Fragments arranged by size: 4672 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3297 3330 4469 4708 5156 5262 5557 6318 7284 Size: 33 1139 239 448 106 295 761 966 Cuts at: 7284 7369 8440 3297 Size: 85 1071 4080 Fragments arranged by size: 4080 1139 1071 966 761 448 295 239 106 85 33 BsmI GAATG_Cn' Cuts at: 2407 4815 6214 6734 6827 2407 Size: 2408 1399 520 93 4803 Fragments arranged by size: 4803 2408 1399 520 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2632 2820 2840 2887 3086 3259 Size: 399 290 552 188 20 47 199 173 Cuts at: 3259 3297 3330 3351 3702 3900 4469 4708 4858 Size: 38 33 21 351 198 569 239 150 Cuts at: 4858 5156 5262 5557 5618 5836 6318 6507 6542 Size: 298 106 295 61 218 482 189 35 Cuts at: 6542 7284 7369 8440 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 552 482 399 351 298 295 290 239 218 199 198 189 188 173 150 106 85 61 47 38 35 33 21 20 BspEI T'CCGG_A Cuts at: 2265 8886 2265 Size: 6621 2602 BspHI T'CATG_A Cuts at: 7112 8842 7112 Size: 1730 7493 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3291 3668 4685 6372 6578 844 Size: 2447 377 1017 1687 206 3489 Fragments arranged by size: 3489 2447 1687 1017 377 206 BsrBI CCG'CTC Cuts at: 1394 1823 2125 3753 7110 1394 Size: 429 302 1628 3357 3507 Fragments arranged by size: 3507 3357 1628 429 302 BsrDI GCAATG_nn' Cuts at: 65 1248 2461 7718 7892 65 Size: 1183 1213 5257 174 1396 Fragments arranged by size: 5257 1396 1213 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 2709 3765 4794 7867 8054 1700 Size: 110 221 678 1056 1029 3073 187 2869 Fragments arranged by size: 3073 2869 1056 1029 678 221 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9223 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9223 BssSI C'ACGA_G Cuts at: 7283 8295 7283 Size: 1012 8211 BstAPI GCAn_nnn'nTGC Cuts at: 794 3292 794 Size: 2498 6725 BstBI TT'CG_AA Cuts at: 2007 8027 2007 Size: 6020 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9223 BstXI CCAn_nnnn'nTGG Cuts at: 2341 3460 3586 4094 2341 Size: 1119 126 508 7470 Fragments arranged by size: 7470 1119 508 126 BstYI r'GATC_y Cuts at: 1972 3125 5290 5708 6553 6920 7305 7322 8774 Size: 1153 2165 418 845 367 385 17 1452 Cuts at: 8774 8860 8872 9218 1972 Size: 86 12 346 1977 Fragments arranged by size: 2165 1977 1452 1153 845 418 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2639 2731 5565 6369 2639 Size: 92 2834 804 5493 Fragments arranged by size: 5493 2834 804 92 BtgI C'CryG_G Cuts at: 513 1065 2022 3633 4186 4245 4497 513 Size: 552 957 1611 553 59 252 5239 Fragments arranged by size: 5239 1611 957 553 552 252 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3230 487 Size: 683 56 2004 6480 Fragments arranged by size: 6480 2004 683 56 BtsI GCAGTG_nn' Cuts at: 2447 3918 4699 5177 6739 7533 7553 2447 Size: 1471 781 478 1562 794 20 4117 Fragments arranged by size: 4117 1562 1471 794 781 478 20 ClaI AT'CG_AT Cuts at: 1554 6225 1554 Size: 4671 4552 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9188 DraI TTT'AAA Cuts at: 6527 6527 Size: 9223 DraIII CAC_nnn'GTG Cuts at: 4125 5636 4125 Size: 1511 7712 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 6161 8230 809 Size: 507 3653 1192 2069 1802 Fragments arranged by size: 3653 2069 1802 1192 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2331 2604 2707 Size: 54 1425 50 275 18 501 273 103 Cuts at: 2707 3588 4084 4096 5602 5880 6670 7559 9188 Size: 881 496 12 1506 278 790 889 1629 Cuts at: 9188 8 Size: 43 Fragments arranged by size: 1629 1506 1425 889 881 790 501 496 278 275 273 103 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 6670 1537 Size: 275 4858 4090 Fragments arranged by size: 4858 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3767 3879 3942 3986 4350 5457 5463 5469 Size: 2414 112 63 44 364 1107 6 6 Cuts at: 5469 6459 7153 1353 Size: 990 694 3423 Fragments arranged by size: 3423 2414 1107 990 694 364 112 63 44 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3784 5782 7810 8184 8330 387 Size: 1064 138 2195 1998 2028 374 146 1280 Fragments arranged by size: 2195 2028 1998 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3328 4467 4706 5154 5260 3328 Size: 1139 239 448 106 7291 Fragments arranged by size: 7291 1139 448 239 106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2363 2376 2377 2406 2528 2835 2855 Size: 393 731 13 1 29 122 307 20 Cuts at: 2855 2863 3815 4194 4453 4556 5118 5630 5811 Size: 8 952 379 259 103 562 512 181 Cuts at: 5811 6329 6461 7286 7883 8670 8938 1239 Size: 518 132 825 597 787 268 1524 Fragments arranged by size: 1524 952 825 787 731 597 562 518 512 393 379 307 268 259 181 132 122 103 29 20 13 8 1 EcoNI CCTnn'n_nnAGG Cuts at: 3414 5826 5942 6074 6368 3414 Size: 2412 116 132 294 6269 Fragments arranged by size: 6269 2412 294 132 116 EcoO109I rG'GnC_Cy Cuts at: 2040 2239 2698 2816 2836 3233 3741 3845 4253 Size: 199 459 118 20 397 508 104 408 Cuts at: 4253 4674 5572 5832 5833 6148 6503 2040 Size: 421 898 260 1 315 355 4760 Fragments arranged by size: 4760 898 508 459 421 408 397 355 315 260 199 118 104 20 1 EcoRI G'AATT_C Cuts at: 6532 6910 6532 Size: 378 8845 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3865 5020 5332 6151 3865 Size: 1155 312 819 6937 Fragments arranged by size: 6937 1155 819 312 FseI GG_CCGG'CC Cuts at: 2713 2713 Size: 9223 FspI TGC'GCA Cuts at: 5183 7729 5183 Size: 2546 6677 HaeII r_GCGC'y Cuts at: 1678 4492 5222 8370 1678 Size: 2814 730 3148 2531 Fragments arranged by size: 3148 2814 2531 730 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3455 3487 4130 4162 4477 Size: 32 135 32 1883 32 643 32 315 Cuts at: 4477 4509 4695 4727 5270 5302 5639 5671 7864 Size: 32 186 32 543 32 337 32 2193 Cuts at: 7864 7896 7938 7970 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2193 1883 643 543 337 315 186 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5956 6166 6567 6813 669 Size: 988 281 4018 210 401 246 3079 Fragments arranged by size: 4018 3079 988 401 281 246 210 HindIII A'AGCT_T Cuts at: 748 748 Size: 9223 HpaI GTT'AAC Cuts at: 6813 6813 Size: 9223 KasI G'GCGC_C Cuts at: 4488 5218 4488 Size: 730 8493 KpnI G_GTAC'C Cuts at: 6548 6548 Size: 9223 MfeI C'AATT_G Cuts at: 6822 6822 Size: 9223 MluI A'CGCG_T Cuts at: 9099 9099 Size: 9223 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3103 3189 4284 5268 8337 8521 1305 Size: 1798 86 1095 984 3069 184 2007 Fragments arranged by size: 3069 2007 1798 1095 984 184 86 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 3590 4086 4098 5604 Size: 54 1425 343 501 1257 496 12 1506 Cuts at: 5604 5882 10 Size: 278 3351 Fragments arranged by size: 3351 1506 1425 1257 501 496 343 278 54 12 MslI CAynn'nnrTG Cuts at: 518 1166 2369 3859 4653 5673 7181 7540 7699 Size: 648 1203 1490 794 1020 1508 359 159 Cuts at: 7699 518 Size: 2042 Fragments arranged by size: 2042 1508 1490 1203 1020 794 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 3268 3859 4473 6277 6639 7316 8464 Size: 459 1203 591 614 1804 362 677 1148 Cuts at: 8464 8709 1606 Size: 245 2120 Fragments arranged by size: 2120 1804 1203 1148 677 614 591 459 362 245 NaeI GCC'GGC Cuts at: 2711 2711 Size: 9223 NarI GG'CG_CC Cuts at: 4489 5219 4489 Size: 730 8493 NcoI C'CATG_G Cuts at: 513 1065 2022 4245 4497 513 Size: 552 957 2223 252 5239 Fragments arranged by size: 5239 2223 957 552 252 NdeI CA'TA_TG Cuts at: 387 387 Size: 9223 NgoMIV G'CCGG_C Cuts at: 2709 2709 Size: 9223 NheI G'CTAG_C Cuts at: 1052 3193 1052 Size: 2141 7082 NotI GC'GGCC_GC Cuts at: 6670 6670 Size: 9223 NsiI A_TGCA'T Cuts at: 2376 4817 2376 Size: 2441 6782 NspI r_CATG'y Cuts at: 1132 1229 2330 2566 3293 4172 5111 6581 8126 Size: 97 1101 236 727 879 939 1470 1545 Cuts at: 8126 1132 Size: 2229 Fragments arranged by size: 2229 1545 1470 1101 939 879 727 236 97 PasI CC'CwG_GG Cuts at: 1348 2082 2684 1348 Size: 734 602 7887 Fragments arranged by size: 7887 734 602 PciI A'CATG_T Cuts at: 2562 8122 2562 Size: 5560 3663 PflMI CCAn_nnn'nTGG Cuts at: 1108 2222 4498 5965 1108 Size: 1114 2276 1467 4366 Fragments arranged by size: 4366 2276 1467 1114 PfoI T'CCnGG_A Cuts at: 3549 3549 Size: 9223 PmeI GTTT'AAAC Cuts at: 6527 6527 Size: 9223 PmlI CAC'GTG Cuts at: 6319 6319 Size: 9223 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5611 5643 7292 7324 8805 8837 5611 Size: 32 1649 32 1481 32 5997 Fragments arranged by size: 5997 1649 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2040 3233 3741 3845 4674 5572 6148 2040 Size: 1193 508 104 829 898 576 5115 Fragments arranged by size: 5115 1193 898 829 576 508 104 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9223 PsiI TTA'TAA Cuts at: 6793 6793 Size: 9223 PspOMI G'GGCC_C Cuts at: 2816 2836 5832 6503 2816 Size: 20 2996 671 5536 Fragments arranged by size: 5536 2996 671 20 PspXI vC'TCGA_Gb Cuts at: 4859 4859 Size: 9223 PstI C_TGCA'G Cuts at: 830 3190 3288 4694 6575 830 Size: 2360 98 1406 1881 3478 Fragments arranged by size: 3478 2360 1881 1406 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9223 PvuII CAG'CTG Cuts at: 2065 3268 3859 4473 6277 2065 Size: 1203 591 614 1804 5011 Fragments arranged by size: 5011 1804 1203 614 591 SacI G_AGCT'C Cuts at: 3330 4469 4708 5156 5262 3330 Size: 1139 239 448 106 7291 Fragments arranged by size: 7291 1139 448 239 106 SalI G'TCGA_C Cuts at: 1936 6565 1936 Size: 4629 4594 SanDI GG'GwC_CC Cuts at: 3741 3845 3741 Size: 104 9119 SapI GCTCTTCn'nnn_ Cuts at: 3986 6459 3986 Size: 2473 6750 SbfI CC_TGCA'GG Cuts at: 6575 6575 Size: 9223 ScaI AGT'ACT Cuts at: 7471 9183 7471 Size: 1712 7511 SexAI A'CCwGG_T Cuts at: 4121 4121 Size: 9223 SfcI C'TryA_G Cuts at: 826 1045 2590 2661 3186 3284 3411 4440 4474 Size: 219 1545 71 525 98 127 1029 34 Cuts at: 4474 4690 6571 7706 8387 8578 826 Size: 216 1881 1135 681 191 1471 Fragments arranged by size: 1881 1545 1471 1135 1029 681 525 219 216 191 127 98 71 34 SfoI GGC'GCC Cuts at: 4490 5220 4490 Size: 730 8493 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9223 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9223 SmaI CCC'GGG Cuts at: 1872 3746 5234 6200 6550 1872 Size: 1874 1488 966 350 4545 Fragments arranged by size: 4545 1874 1488 966 350 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 2273 4299 4859 5036 5617 Size: 197 926 153 177 2026 560 177 581 Cuts at: 5617 7327 8228 8490 820 Size: 1710 901 262 1553 Fragments arranged by size: 2026 1710 1553 926 901 581 560 262 197 177 177 153 SnaBI TAC'GTA Cuts at: 493 6520 7014 493 Size: 6027 494 2702 Fragments arranged by size: 6027 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9223 SphI G_CATG'C Cuts at: 1132 6581 1132 Size: 5449 3774 SspI AAT'ATT Cuts at: 5 52 5872 7147 5 Size: 47 5820 1275 2081 Fragments arranged by size: 5820 2081 1275 47 StuI AGG'CCT Cuts at: 1517 2876 3107 4093 5315 1517 Size: 1359 231 986 1222 5425 Fragments arranged by size: 5425 1359 1222 986 231 StyI C'CwwG_G Cuts at: 513 1065 2022 3702 4245 4497 5092 5883 5938 Size: 552 957 1680 543 252 595 791 55 Cuts at: 5938 6070 6300 6662 513 Size: 132 230 362 3074 Fragments arranged by size: 3074 1680 957 791 595 552 543 362 252 230 132 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3710 5190 7250 7267 7420 7605 8050 Size: 1255 2130 1480 2060 17 153 185 445 Cuts at: 8050 325 Size: 1498 Fragments arranged by size: 2130 2060 1498 1480 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5355 7469 9181 Size: 274 80 33 51 1443 3378 2114 1712 Cuts at: 9181 96 Size: 138 Fragments arranged by size: 3378 2114 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5622 5654 9183 9215 5622 Size: 32 3529 32 5630 Fragments arranged by size: 5630 3529 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3361 5284 1316 Size: 2045 1923 5255 Fragments arranged by size: 5255 2045 1923 XbaI T'CTAG_A Cuts at: 4618 6559 4618 Size: 1941 7282 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 2894 6140 2494 Size: 400 3246 5577 Fragments arranged by size: 5577 3246 400 XhoI C'TCGA_G Cuts at: 1943 4859 5617 1943 Size: 2916 758 5549 Fragments arranged by size: 5549 2916 758 XmaI C'CCGG_G Cuts at: 1870 3744 5232 6198 6548 1870 Size: 1874 1488 966 350 4545 Fragments arranged by size: 4545 1874 1488 966 350 XmnI GAAnn'nnTTC Cuts at: 6010 7352 6010 Size: 1342 7881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7031 276 Size: 53 83 186 936 5497 2468 Fragments arranged by size: 5497 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BsiWI BsmBI BstZ17I EcoRV FspAI NruI PacI PsrI RsrII SacII SfiI SrfI SwaI