Restriction map for
pFN21ASDA1202
(Linear) (Six-base) MAPSORT of: KIAA1202.seq Check: 6950 from: 1 to: 4494
KIAA1202.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1268 1645 4494
Size: 1268 377 2849
Fragments arranged by size:
2849 1268 377
AccI GT'mk_AC
Cuts at: 0 4470 4494
Size: 4470 24
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 354 383 832 2533 3095 3788 4306
Size: 353 1 29 449 1701 562 693 518
Cuts at: 4306 4494
Size: 188
Fragments arranged by size:
1701 693 562 518 449 353 188 29
1
AflII C'TTAA_G
Cuts at: 0 73 4494
Size: 73 4421
AflIII A'CryG_T
Cuts at: 0 407 539 4494
Size: 407 132 3955
Fragments arranged by size:
3955 407 132
AleI CACnn'nnGTG
Cuts at: 0 1836 2630 4494
Size: 1836 794 1864
Fragments arranged by size:
1864 1836 794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1598 4494
Size: 1598 2896
AlwNI CAG_nnn'CTG
Cuts at: 0 1248 2134 2654 4254 4494
Size: 1248 886 520 1600 240
Fragments arranged by size:
1600 1248 886 520 240
ApaI G_GGCC'C
Cuts at: 0 797 817 3813 4484 4494
Size: 797 20 2996 671 10
Fragments arranged by size:
2996 797 671 20 10
ApaLI G'TGCA_C
Cuts at: 0 1270 4494
Size: 1270 3224
ApoI r'AATT_y
Cuts at: 0 1784 2601 2896 4096 4494
Size: 1784 817 295 1200 398
Fragments arranged by size:
1784 1200 817 398 295
AvaI C'yCGr_G
Cuts at: 0 1721 2836 3209 3594 4175 4435 4494
Size: 1721 1115 373 385 581 260 59
Fragments arranged by size:
1721 1115 581 385 373 260 59
AvrII C'CTAG_G
Cuts at: 0 3915 4494
Size: 3915 579
BamHI G'GATC_C
Cuts at: 0 1102 4494
Size: 1102 3392
BanI G'GyrC_C
Cuts at: 0 54 1325 2465 2774 2853 3195 4494
Size: 54 1271 1140 309 79 342 1299
Fragments arranged by size:
1299 1271 1140 342 309 79 54
BanII G_rGCy'C
Cuts at: 0 797 817 864 1063 1307 1679 1877 2446
Size: 797 20 47 199 244 372 198 569
Cuts at: 2446 2685 3133 3239 3595 3813 4484 4494
Size: 239 448 106 356 218 671 10
Fragments arranged by size:
797 671 569 448 372 356 244 239
218 199 198 106 47 20 10
BbeI G_GCGC'C
Cuts at: 0 2469 3199 4494
Size: 2469 730 1295
Fragments arranged by size:
2469 1295 730
BbsI GAAGACnn'nnnn_
Cuts at: 0 1982 2944 4494
Size: 1982 962 1550
Fragments arranged by size:
1982 1550 962
BbvCI CC'TCA_GC
Cuts at: 0 2002 2822 3571 4494
Size: 2002 820 749 923
Fragments arranged by size:
2002 923 820 749
BciVI GTATCCnnnnn_n'
Cuts at: 0 775 4494
Size: 775 3719
BfrBI ATG'CAT
Cuts at: 0 351 2792 4494
Size: 351 2441 1702
Fragments arranged by size:
2441 1702 351
BglI GCCn_nnn'nGGC
Cuts at: 0 1058 4494
Size: 1058 3436
BlpI GC'TnA_GC
Cuts at: 0 3704 4494
Size: 3704 790
Bme1580I G_kGCm'C
Cuts at: 0 57 609 797 817 1236 1274 1328 2835
Size: 57 552 188 20 419 38 54 1507
Cuts at: 2835 3813 4484 4494
Size: 978 671 10
Fragments arranged by size:
1507 978 671 552 419 188 57 54
38 20 10
BmgBI CAC'GTC
Cuts at: 0 408 4494
Size: 408 4086
BmrI ACTGGGnnnn_n'
Cuts at: 0 674 3255 3333 3492 4494
Size: 674 2581 78 159 1002
Fragments arranged by size:
2581 1002 674 159 78
BmtI G_CTAG'C
Cuts at: 0 1174 4494
Size: 1174 3320
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 651 883 1972 2201 3147 4494
Size: 651 232 1089 229 946 1347
Fragments arranged by size:
1347 1089 946 651 232 229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 505 812 840 1792 2171 2430 3607
Size: 340 165 307 28 952 379 259 1177
Cuts at: 3607 4438 4494
Size: 831 56
Fragments arranged by size:
1177 952 831 379 340 307 259 165
56 28
Bpu10I CC'TnA_GC
Cuts at: 0 1175 2002 2822 3571 3695 4494
Size: 1175 827 820 749 124 799
Fragments arranged by size:
1175 827 820 799 749 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 2261 3034 4494
Size: 235 2026 773 1460
Fragments arranged by size:
2026 1460 773 235
BsaI GGTCTCn'nnnn_
Cuts at: 0 697 1858 2189 2400 3250 4335 4494
Size: 697 1161 331 211 850 1085 159
Fragments arranged by size:
1161 1085 850 697 331 211 159
BsaAI yAC'GTr
Cuts at: 0 4296 4494
Size: 4296 198
BsaHI Gr'CG_yC
Cuts at: 0 2466 3196 4494
Size: 2466 730 1298
Fragments arranged by size:
2466 1298 730
BsaWI w'CCGG_w
Cuts at: 0 242 4494
Size: 242 4252
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2107 2137 2672 2702 4494
Size: 2107 30 535 30 1792
Fragments arranged by size:
2107 1792 535 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 282 640 859 1806 2115 3136 3290 3293
Size: 282 358 219 947 309 1021 154 3
Cuts at: 3293 3302 3305 3421 3424 3427 3430 3433 3436
Size: 9 3 116 3 3 3 3 3
Cuts at: 3436 3469 3472 3475 3478 3487 3490 3493 3496
Size: 33 3 3 3 9 3 3 3
Cuts at: 3496 3499 3559 3937 4069 4435 4494
Size: 3 60 378 132 366 59
Fragments arranged by size:
1021 947 378 366 358 309 282 219
154 132 116 60 59 33 9 9
3 3 3 3 3 3 3 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 748 1802 1939 2281 3021 3353 3618 4007
Size: 748 1054 137 342 740 332 265 389
Cuts at: 4007 4483 4494
Size: 476 11
Fragments arranged by size:
1054 748 740 476 389 342 332 265
137 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 436 1707 2911 3368 4494
Size: 58 378 1271 1204 457 1126
Fragments arranged by size:
1271 1204 1126 457 378 58
BsiHKAI G_wGCw'C
Cuts at: 0 1274 1307 2446 2685 3133 3239 3534 4295
Size: 1274 33 1139 239 448 106 295 761
Cuts at: 4295 4494
Size: 199
Fragments arranged by size:
1274 1139 761 448 295 239 199 106
33
BsmI GAATG_Cn'
Cuts at: 0 384 2792 4191 4494
Size: 384 2408 1399 303
Fragments arranged by size:
2408 1399 384 303
Bsp1286I G_dGCh'C
Cuts at: 0 57 609 797 817 864 1063 1236 1274
Size: 57 552 188 20 47 199 173 38
Cuts at: 1274 1307 1328 1679 1877 2446 2685 2835 3133
Size: 33 21 351 198 569 239 150 298
Cuts at: 3133 3239 3534 3595 3813 4295 4484 4494
Size: 106 295 61 218 482 189 10
Fragments arranged by size:
569 552 482 351 298 295 239 218
199 198 189 188 173 150 106 61
57 47 38 33 21 20 10
BspEI T'CCGG_A
Cuts at: 0 242 4494
Size: 242 4252
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1268 1645 2662 4349 4494
Size: 1268 377 1017 1687 145
Fragments arranged by size:
1687 1268 1017 377 145
BsrBI CCG'CTC
Cuts at: 0 102 1730 4494
Size: 102 1628 2764
Fragments arranged by size:
2764 1628 102
BsrDI GCAATG_nn'
Cuts at: 0 438 4494
Size: 438 4056
BsrFI r'CCGG_y
Cuts at: 0 8 686 1742 2771 4494
Size: 8 678 1056 1029 1723
Fragments arranged by size:
1723 1056 1029 678 8
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1269 4494
Size: 1269 3225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 1437 1563 2071 4494
Size: 318 1119 126 508 2423
Fragments arranged by size:
2423 1119 508 318 126
BstYI r'GATC_y
Cuts at: 0 1102 3267 3685 4494
Size: 1102 2165 418 809
Fragments arranged by size:
2165 1102 809 418
Bsu36I CC'TnA_GG
Cuts at: 0 616 708 3542 4346 4494
Size: 616 92 2834 804 148
Fragments arranged by size:
2834 804 616 148 92
BtgI C'CryG_G
Cuts at: 0 1610 2163 2222 2474 4494
Size: 1610 553 59 252 2020
Fragments arranged by size:
2020 1610 553 252 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1207 4494
Size: 1207 3287
BtsI GCAGTG_nn'
Cuts at: 0 424 1895 2676 3154 4494
Size: 424 1471 781 478 1340
Fragments arranged by size:
1471 1340 781 478 424
ClaI AT'CG_AT
Cuts at: 0 4202 4494
Size: 4202 292
DraIII CAC_nnn'GTG
Cuts at: 0 2102 3613 4494
Size: 2102 1511 881
Fragments arranged by size:
2102 1511 881
DrdI GACnn_nn'nnGTC
Cuts at: 0 2946 4138 4494
Size: 2946 1192 356
Fragments arranged by size:
2946 1192 356
EaeI y'GGCC_r
Cuts at: 0 308 581 684 1565 2061 2073 3579 3857
Size: 308 273 103 881 496 12 1506 278
Cuts at: 3857 4494
Size: 637
Fragments arranged by size:
1506 881 637 496 308 278 273 103
12
EarI CTCTTCn'nnn_
Cuts at: 0 1744 1856 1919 1963 2327 3434 3440 3446
Size: 1744 112 63 44 364 1107 6 6
Cuts at: 3446 4436 4494
Size: 990 58
Fragments arranged by size:
1744 1107 990 364 112 63 58 44
6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1761 3759 4494
Size: 1761 1998 735
Fragments arranged by size:
1998 1761 735
EcoICRI GAG'CTC
Cuts at: 0 1305 2444 2683 3131 3237 4494
Size: 1305 1139 239 448 106 1257
Fragments arranged by size:
1305 1257 1139 448 239 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 353 354 383 505 812 832 840
Size: 340 13 1 29 122 307 20 8
Cuts at: 840 1792 2171 2430 2533 3095 3607 3788 4306
Size: 952 379 259 103 562 512 181 518
Cuts at: 4306 4438 4494
Size: 132 56
Fragments arranged by size:
952 562 518 512 379 340 307 259
181 132 122 103 56 29 20 13
8 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1391 3803 3919 4051 4345 4494
Size: 1391 2412 116 132 294 149
Fragments arranged by size:
2412 1391 294 149 132 116
EcoO109I rG'GnC_Cy
Cuts at: 0 17 216 675 793 813 1210 1718 1822
Size: 17 199 459 118 20 397 508 104
Cuts at: 1822 2230 2651 3549 3809 3810 4125 4480 4494
Size: 408 421 898 260 1 315 355 14
Fragments arranged by size:
898 508 459 421 408 397 355 315
260 199 118 104 20 17 14 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1842 2997 3309 4128 4494
Size: 1842 1155 312 819 366
Fragments arranged by size:
1842 1155 819 366 312
FseI GG_CCGG'CC
Cuts at: 0 690 4494
Size: 690 3804
FspI TGC'GCA
Cuts at: 0 3160 4494
Size: 3160 1334
HaeII r_GCGC'y
Cuts at: 0 2469 3199 4494
Size: 2469 730 1295
Fragments arranged by size:
2469 1295 730
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1432 1464 2107 2139 2454 2486 2672 2704
Size: 1432 32 643 32 315 32 186 32
Cuts at: 2704 3247 3279 3616 3648 4494
Size: 543 32 337 32 846
Fragments arranged by size:
1432 846 643 543 337 315 186 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3933 4143 4494
Size: 3933 210 351
Fragments arranged by size:
3933 351 210
KasI G'GCGC_C
Cuts at: 0 2465 3195 4494
Size: 2465 730 1299
Fragments arranged by size:
2465 1299 730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1080 1166 2261 3245 4494
Size: 1080 86 1095 984 1249
Fragments arranged by size:
1249 1095 1080 984 86
MscI TGG'CCA
Cuts at: 0 310 1567 2063 2075 3581 3859 4494
Size: 310 1257 496 12 1506 278 635
Fragments arranged by size:
1506 1257 635 496 310 278 12
MslI CAynn'nnrTG
Cuts at: 0 346 1836 2630 3650 4494
Size: 346 1490 794 1020 844
Fragments arranged by size:
1490 1020 844 794 346
MspA1I CmG'CkG
Cuts at: 0 42 1245 1836 2450 4254 4494
Size: 42 1203 591 614 1804 240
Fragments arranged by size:
1804 1203 614 591 240 42
NaeI GCC'GGC
Cuts at: 0 688 4494
Size: 688 3806
NarI GG'CG_CC
Cuts at: 0 2466 3196 4494
Size: 2466 730 1298
Fragments arranged by size:
2466 1298 730
NcoI C'CATG_G
Cuts at: 0 2222 2474 4494
Size: 2222 252 2020
Fragments arranged by size:
2222 2020 252
NgoMIV G'CCGG_C
Cuts at: 0 686 4494
Size: 686 3808
NheI G'CTAG_C
Cuts at: 0 1170 4494
Size: 1170 3324
NsiI A_TGCA'T
Cuts at: 0 353 2794 4494
Size: 353 2441 1700
Fragments arranged by size:
2441 1700 353
NspI r_CATG'y
Cuts at: 0 307 543 1270 2149 3088 4494
Size: 307 236 727 879 939 1406
Fragments arranged by size:
1406 939 879 727 307 236
PasI CC'CwG_GG
Cuts at: 0 59 661 4494
Size: 59 602 3833
Fragments arranged by size:
3833 602 59
PciI A'CATG_T
Cuts at: 0 539 4494
Size: 539 3955
PflMI CCAn_nnn'nTGG
Cuts at: 0 199 2475 3942 4494
Size: 199 2276 1467 552
Fragments arranged by size:
2276 1467 552 199
PfoI T'CCnGG_A
Cuts at: 0 1526 4494
Size: 1526 2968
PmlI CAC'GTG
Cuts at: 0 4296 4494
Size: 4296 198
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3588 3620 4494
Size: 3588 32 874
Fragments arranged by size:
3588 874 32
PpuMI rG'GwC_Cy
Cuts at: 0 17 1210 1718 1822 2651 3549 4125 4494
Size: 17 1193 508 104 829 898 576 369
Fragments arranged by size:
1193 898 829 576 508 369 104 17
PspOMI G'GGCC_C
Cuts at: 0 793 813 3809 4480 4494
Size: 793 20 2996 671 14
Fragments arranged by size:
2996 793 671 20 14
PspXI vC'TCGA_Gb
Cuts at: 0 2836 4494
Size: 2836 1658
PstI C_TGCA'G
Cuts at: 0 1167 1265 2671 4494
Size: 1167 98 1406 1823
Fragments arranged by size:
1823 1406 1167 98
PvuII CAG'CTG
Cuts at: 0 42 1245 1836 2450 4254 4494
Size: 42 1203 591 614 1804 240
Fragments arranged by size:
1804 1203 614 591 240 42
SacI G_AGCT'C
Cuts at: 0 1307 2446 2685 3133 3239 4494
Size: 1307 1139 239 448 106 1255
Fragments arranged by size:
1307 1255 1139 448 239 106
SanDI GG'GwC_CC
Cuts at: 0 1718 1822 4494
Size: 1718 104 2672
Fragments arranged by size:
2672 1718 104
SapI GCTCTTCn'nnn_
Cuts at: 0 1963 4436 4494
Size: 1963 2473 58
Fragments arranged by size:
2473 1963 58
SexAI A'CCwGG_T
Cuts at: 0 2098 4494
Size: 2098 2396
SfcI C'TryA_G
Cuts at: 0 567 638 1163 1261 1388 2417 2451 2667
Size: 567 71 525 98 127 1029 34 216
Cuts at: 2667 4494
Size: 1827
Fragments arranged by size:
1827 1029 567 525 216 127 98 71
34
SfoI GGC'GCC
Cuts at: 0 2467 3197 4494
Size: 2467 730 1297
Fragments arranged by size:
2467 1297 730
SmaI CCC'GGG
Cuts at: 0 1723 3211 4177 4494
Size: 1723 1488 966 317
Fragments arranged by size:
1723 1488 966 317
SmlI C'TyrA_G
Cuts at: 0 73 250 2276 2836 3013 3594 4494
Size: 73 177 2026 560 177 581 900
Fragments arranged by size:
2026 900 581 560 177 177 73
SspI AAT'ATT
Cuts at: 0 3849 4494
Size: 3849 645
StuI AGG'CCT
Cuts at: 0 853 1084 2070 3292 4494
Size: 853 231 986 1222 1202
Fragments arranged by size:
1222 1202 986 853 231
StyI C'CwwG_G
Cuts at: 0 1679 2222 2474 3069 3860 3915 4047 4277
Size: 1679 543 252 595 791 55 132 230
Cuts at: 4277 4494
Size: 217
Fragments arranged by size:
1679 791 595 543 252 230 217 132
55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1687 3167 4494
Size: 1687 1480 1327
Fragments arranged by size:
1687 1480 1327
TatI w'GTAC_w
Cuts at: 0 3332 4494
Size: 3332 1162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3599 3631 4494
Size: 3599 32 863
Fragments arranged by size:
3599 863 32
Tth111I GACn'n_nGTC
Cuts at: 0 1338 3261 4494
Size: 1338 1923 1233
Fragments arranged by size:
1923 1338 1233
XbaI T'CTAG_A
Cuts at: 0 2595 4494
Size: 2595 1899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 871 4117 4494
Size: 471 400 3246 377
Fragments arranged by size:
3246 471 400 377
XhoI C'TCGA_G
Cuts at: 0 2836 3594 4494
Size: 2836 758 900
Fragments arranged by size:
2836 900 758
XmaI C'CCGG_G
Cuts at: 0 1721 3209 4175 4494
Size: 1721 1488 966 319
Fragments arranged by size:
1721 1488 966 319
XmnI GAAnn'nnTTC
Cuts at: 0 3987 4494
Size: 3987 507
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BciVI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI FspI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmlI PpiI
PpuMI PspOMI PspXI PstI PvuII SacI SanDI SapI
SexAI SfcI SfoI SmaI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AloI AscI
AseI AsiSI BaeI BcgI BclI BglII BsaBI BsiEI
BsiWI BsmBI BspHI BsrGI BssHII BssSI BstBI BstEII
BstZ17I CspCI DraI EagI EcoRI EcoRV FspAI HindIII
HpaI KpnI MfeI MluI NdeI NotI NruI PacI
PmeI PshAI PsiI PsrI PvuI RsrII SacII SalI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SwaI ZraI