(Circular) (Six-base) MAPSORT of: pFN21ASDA1197.seq Check: 5736 from: 1 to: 8995 pFN21ASDA1197 8995 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3535 5287 3535 Size: 1752 7243 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6805 278 Size: 53 83 186 936 5269 2468 Fragments arranged by size: 5269 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6338 1937 Size: 4401 4594 Acc65I G'GTAC_C Cuts at: 3979 6316 3979 Size: 2337 6658 AclI AA'CG_TT Cuts at: 1563 4238 7122 7495 1563 Size: 2675 2884 373 3063 Fragments arranged by size: 3063 2884 2675 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2963 3210 4311 4959 6125 7058 8442 8710 Size: 1331 247 1101 648 1166 933 1384 268 Cuts at: 8710 1632 Size: 1917 Fragments arranged by size: 1917 1384 1331 1166 1101 933 648 268 247 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8798 AflIII A'CryG_T Cuts at: 1203 2324 6072 7894 8871 1203 Size: 1121 3748 1822 977 1327 Fragments arranged by size: 3748 1822 1327 1121 977 AgeI A'CCGG_T Cuts at: 7826 7826 Size: 8995 AhdI GACnn_n'nnGTC Cuts at: 7724 7724 Size: 8995 AleI CACnn'nnGTG Cuts at: 1166 3785 1166 Size: 2619 6376 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3337 3507 1184 Size: 2153 170 6672 Fragments arranged by size: 6672 2153 170 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5963 5995 5963 Size: 32 8963 AlwNI CAG_nnn'CTG Cuts at: 2224 3839 4045 4561 5336 6005 6738 8310 2224 Size: 1615 206 516 775 669 733 1572 2909 Fragments arranged by size: 2909 1615 1572 775 733 669 516 206 ApaLI G'TGCA_C Cuts at: 7052 8208 7052 Size: 1156 7839 ApoI r'AATT_y Cuts at: 1493 2116 2363 3642 4276 6008 6304 6530 6682 Size: 623 247 1279 634 1732 296 226 152 Cuts at: 6682 8791 1493 Size: 2109 1697 Fragments arranged by size: 2109 1732 1697 1279 634 623 296 247 226 152 AseI AT'TA_AT Cuts at: 160 1032 2763 3507 5510 7549 160 Size: 872 1731 744 2003 2039 1606 Fragments arranged by size: 2039 2003 1731 1606 872 744 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8995 AvaI C'yCGr_G Cuts at: 1870 1943 2124 5804 5885 6320 1870 Size: 73 181 3680 81 435 4545 Fragments arranged by size: 4545 3680 435 181 81 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2322 2355 3147 3180 2322 Size: 33 792 33 8137 Fragments arranged by size: 8137 792 33 33 BamHI G'GATC_C Cuts at: 4214 6325 6692 4214 Size: 2111 367 6517 Fragments arranged by size: 6517 2111 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3979 4427 5709 6316 7771 Size: 325 217 627 2192 448 1282 607 1455 Cuts at: 7771 618 Size: 1842 Fragments arranged by size: 2192 1842 1455 1282 627 607 448 325 217 BanII G_rGCy'C Cuts at: 1391 5805 6314 1391 Size: 4414 509 4072 Fragments arranged by size: 4414 4072 509 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2288 3036 4817 4950 5357 928 Size: 971 389 748 1781 133 407 4566 Fragments arranged by size: 4566 1781 971 748 407 389 133 BbvCI CC'TCA_GC Cuts at: 5912 5912 Size: 8995 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5215 5249 7186 7220 1903 Size: 34 3278 34 1937 34 3678 Fragments arranged by size: 3678 3278 1937 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2554 5125 6152 6887 8097 2554 Size: 2571 1027 735 1210 3452 Fragments arranged by size: 3452 2571 1210 1027 735 BclI T'GATC_A Cuts at: 1548 3789 1548 Size: 2241 6754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4271 7606 136 Size: 107 122 71 631 605 2599 3335 1525 Fragments arranged by size: 3335 2599 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3591 8990 3591 Size: 5399 3596 BlpI GC'TnA_GC Cuts at: 4155 4405 6412 4155 Size: 250 2007 6738 Fragments arranged by size: 6738 2007 250 Bme1580I G_kGCm'C Cuts at: 1790 3828 4604 5813 7056 8212 1790 Size: 2038 776 1209 1243 1156 2573 Fragments arranged by size: 2573 2038 1243 1209 1156 776 BmgBI CAC'GTC Cuts at: 1318 5070 5779 1318 Size: 3752 709 4534 Fragments arranged by size: 4534 3752 709 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3101 3561 3937 Size: 416 53 83 399 24 1684 460 376 Cuts at: 3937 4665 4688 6726 7684 442 Size: 728 23 2038 958 1753 Fragments arranged by size: 2038 1753 1684 958 728 460 416 399 376 83 53 24 23 BmtI G_CTAG'C Cuts at: 1056 3186 1056 Size: 2130 6865 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3453 3948 4379 4563 5122 5232 5292 5313 Size: 2214 495 431 184 559 110 60 21 Cuts at: 5313 5669 5873 7655 1239 Size: 356 204 1782 2579 Fragments arranged by size: 2579 2214 1782 559 495 431 356 204 184 110 60 21 Bpu10I CC'TnA_GC Cuts at: 5912 5912 Size: 8995 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4341 4568 7120 7985 8283 4341 Size: 227 2552 865 298 5053 Fragments arranged by size: 5053 2552 865 298 227 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2530 3616 7658 882 Size: 617 1031 1086 4042 2219 Fragments arranged by size: 4042 2219 1086 1031 617 BsaAI yAC'GTr Cuts at: 493 1204 3478 3823 6292 6786 493 Size: 711 2274 345 2469 494 2702 Fragments arranged by size: 2702 2469 2274 711 494 345 BsaBI GATnn'nnATC Cuts at: 2927 5939 6038 8989 2927 Size: 3012 99 2951 2933 Fragments arranged by size: 3012 2951 2933 99 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6802 7184 7819 275 Size: 53 83 186 936 5269 382 635 1451 Fragments arranged by size: 5269 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2683 5117 5734 7428 7826 8100 8247 8658 2683 Size: 2434 617 1694 398 274 147 411 3020 Fragments arranged by size: 3020 2434 1694 617 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3451 3481 3578 3608 1178 Size: 30 165 30 2048 30 97 30 6565 Fragments arranged by size: 6565 2048 165 97 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4449 4452 4455 4458 4461 4464 4467 4470 Size: 3263 3 3 3 3 3 3 3 Cuts at: 4470 4509 4512 4515 4530 5815 5909 5912 1186 Size: 39 3 3 15 1285 94 3 4269 Fragments arranged by size: 4269 3263 1285 94 39 15 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2756 2865 3178 5177 5318 5431 8198 8694 2756 Size: 109 313 1999 141 113 2767 496 3057 Fragments arranged by size: 3057 2767 1999 496 313 141 113 109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 4184 4683 4911 5240 1163 Size: 573 423 2025 499 228 329 4918 Fragments arranged by size: 4918 2025 573 499 423 329 228 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6445 7206 8234 1540 Size: 275 186 4444 761 1028 2301 Fragments arranged by size: 4444 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 7056 7141 8212 7056 Size: 85 1071 7839 Fragments arranged by size: 7839 1071 85 BsmI GAATG_Cn' Cuts at: 2608 6506 6599 2608 Size: 3898 93 5004 Fragments arranged by size: 5004 3898 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2981 2981 Size: 8995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3828 4604 5805 5813 6314 7056 7141 Size: 399 2038 776 1201 8 501 742 85 Cuts at: 7141 8212 1391 Size: 1071 2174 Fragments arranged by size: 2174 2038 1201 1071 776 742 501 399 85 8 BspEI T'CCGG_A Cuts at: 2683 5117 8658 2683 Size: 2434 3541 3020 Fragments arranged by size: 3541 3020 2434 BspHI T'CATG_A Cuts at: 6884 8614 6884 Size: 1730 7265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2282 3535 5042 5287 5868 5994 6350 844 Size: 1438 1253 1507 245 581 126 356 3489 Fragments arranged by size: 3489 1507 1438 1253 581 356 245 126 BsrBI CCG'CTC Cuts at: 1394 1823 6882 1394 Size: 429 5059 3507 Fragments arranged by size: 5059 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2668 3443 5622 7490 7664 65 Size: 1183 1420 775 2179 1868 174 1396 Fragments arranged by size: 2179 1868 1420 1396 1183 775 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3494 7639 7826 1700 Size: 110 1684 4145 187 2869 Fragments arranged by size: 4145 2869 1684 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8995 BssSI C'ACGA_G Cuts at: 5075 7055 8067 5075 Size: 1980 1012 6003 Fragments arranged by size: 6003 1980 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5339 794 Size: 4545 4450 BstBI TT'CG_AA Cuts at: 2007 7799 2007 Size: 5792 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8995 BstXI CCAn_nnnn'nTGG Cuts at: 4261 4637 4261 Size: 376 8619 BstYI r'GATC_y Cuts at: 1972 3591 4041 4214 6325 6692 7077 7094 8546 Size: 1619 450 173 2111 367 385 17 1452 Cuts at: 8546 8632 8644 8990 1972 Size: 86 12 346 1977 Fragments arranged by size: 2111 1977 1619 1452 450 385 367 346 173 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3735 5750 3735 Size: 2015 6980 BtgI C'CryG_G Cuts at: 513 1065 3286 3597 4765 4902 5025 6054 6084 Size: 552 2221 311 1168 137 123 1029 30 Cuts at: 6084 513 Size: 3424 Fragments arranged by size: 3424 2221 1168 1029 552 311 137 123 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2996 5683 487 Size: 683 56 1770 2687 3799 Fragments arranged by size: 3799 2687 1770 683 56 BtsI GCAGTG_nn' Cuts at: 3489 4471 5412 5514 6511 7305 7325 3489 Size: 982 941 102 997 794 20 5159 Fragments arranged by size: 5159 997 982 941 794 102 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2637 2672 5538 5573 529 Size: 35 2073 35 2866 35 3951 Fragments arranged by size: 3951 2866 2073 35 35 35 DraI TTT'AAA Cuts at: 6299 6299 Size: 8995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8002 809 Size: 507 6686 1802 Fragments arranged by size: 6686 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4036 4263 4615 Size: 54 1425 50 275 18 2206 227 352 Cuts at: 4615 6442 7331 8960 8 Size: 1827 889 1629 43 Fragments arranged by size: 2206 1827 1629 1425 889 352 275 227 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6442 1537 Size: 275 4630 4090 Fragments arranged by size: 4630 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2854 4069 4202 5952 6925 1353 Size: 1501 1215 133 1750 973 3423 Fragments arranged by size: 3423 1750 1501 1215 973 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7582 7956 8102 387 Size: 1064 138 5993 374 146 1280 Fragments arranged by size: 5993 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2963 3210 3453 3948 4311 4379 4563 Size: 393 1331 247 243 495 363 68 184 Cuts at: 4563 4959 5122 5232 5292 5313 5669 5873 6125 Size: 396 163 110 60 21 356 204 252 Cuts at: 6125 7058 7655 8442 8710 1239 Size: 933 597 787 268 1524 Fragments arranged by size: 1524 1331 933 787 597 495 396 393 363 356 268 252 247 243 204 184 163 110 68 60 21 EcoNI CCTnn'n_nnAGG Cuts at: 3759 3930 5387 6140 3759 Size: 171 1457 753 6614 Fragments arranged by size: 6614 1457 753 171 EcoO109I rG'GnC_Cy Cuts at: 2742 5857 2742 Size: 3115 5880 EcoRI G'AATT_C Cuts at: 2116 6304 6682 2116 Size: 4188 378 4429 Fragments arranged by size: 4429 4188 378 EcoRV GAT'ATC Cuts at: 2929 4551 6133 2929 Size: 1622 1582 5791 Fragments arranged by size: 5791 1622 1582 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2463 2580 2463 Size: 117 8878 FspI TGC'GCA Cuts at: 7501 7501 Size: 8995 HaeII r_GCGC'y Cuts at: 1678 5067 8142 1678 Size: 3389 3075 2531 Fragments arranged by size: 3389 3075 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2435 2456 2467 Size: 32 135 32 488 32 343 21 11 Cuts at: 2467 2488 3451 3483 4863 4895 5750 5782 5798 Size: 21 963 32 1380 32 855 32 16 Cuts at: 5798 5830 6157 6189 7636 7668 7710 7742 1373 Size: 32 327 32 1447 32 42 32 2626 Fragments arranged by size: 2626 1447 1380 963 855 488 343 327 135 42 32 32 32 32 32 32 32 32 32 32 21 21 16 11 HincII GTy'rAC Cuts at: 669 1657 1938 2712 6339 6585 669 Size: 988 281 774 3627 246 3079 Fragments arranged by size: 3627 3079 988 774 281 246 HindIII A'AGCT_T Cuts at: 748 3114 748 Size: 2366 6629 HpaI GTT'AAC Cuts at: 6585 6585 Size: 8995 KpnI G_GTAC'C Cuts at: 3983 6320 3983 Size: 2337 6658 MfeI C'AATT_G Cuts at: 2109 3720 4122 6594 2109 Size: 1611 402 2472 4510 Fragments arranged by size: 4510 2472 1611 402 MluI A'CGCG_T Cuts at: 8871 8871 Size: 8995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6136 8109 8293 1305 Size: 4831 1973 184 2007 Fragments arranged by size: 4831 2007 1973 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4038 4265 4617 10 Size: 54 1425 343 2206 227 352 4388 Fragments arranged by size: 4388 2206 1425 352 343 227 54 MslI CAynn'nnrTG Cuts at: 518 1166 3251 3389 3785 6953 7312 7471 518 Size: 648 2085 138 396 3168 359 159 2042 Fragments arranged by size: 3168 2085 2042 648 396 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2599 4196 4409 4473 5336 5538 5600 5715 Size: 993 1597 213 64 863 202 62 115 Cuts at: 5715 6121 6411 7088 8236 8481 1606 Size: 406 290 677 1148 245 2120 Fragments arranged by size: 2120 1597 1148 993 863 677 406 290 245 213 202 115 64 62 NaeI GCC'GGC Cuts at: 3496 3496 Size: 8995 NcoI C'CATG_G Cuts at: 513 1065 3286 4765 513 Size: 552 2221 1479 4743 Fragments arranged by size: 4743 2221 1479 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8995 NgoMIV G'CCGG_C Cuts at: 3494 3494 Size: 8995 NheI G'CTAG_C Cuts at: 1052 3182 1052 Size: 2130 6865 NotI GC'GGCC_GC Cuts at: 6442 6442 Size: 8995 NspI r_CATG'y Cuts at: 1132 1229 2328 6353 7898 1132 Size: 97 1099 4025 1545 2229 Fragments arranged by size: 4025 2229 1545 1099 97 PasI CC'CwG_GG Cuts at: 1348 4219 4597 5999 1348 Size: 2871 378 1402 4344 Fragments arranged by size: 4344 2871 1402 378 PciI A'CATG_T Cuts at: 2324 7894 2324 Size: 5570 3425 PflMI CCAn_nnn'nTGG Cuts at: 1108 4045 1108 Size: 2937 6058 PfoI T'CCnGG_A Cuts at: 3925 5646 3925 Size: 1721 7274 PmeI GTTT'AAAC Cuts at: 6299 6299 Size: 8995 PmlI CAC'GTG Cuts at: 3478 3823 3478 Size: 345 8650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2877 2909 7064 7096 8577 8609 2877 Size: 32 4155 32 1481 32 3263 Fragments arranged by size: 4155 3263 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2742 5857 2742 Size: 3115 5880 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8995 PsiI TTA'TAA Cuts at: 6565 6565 Size: 8995 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4513 4545 4513 Size: 32 8963 PstI C_TGCA'G Cuts at: 830 2293 5491 6051 6347 830 Size: 1463 3198 560 296 3478 Fragments arranged by size: 3478 3198 1463 560 296 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8995 PvuII CAG'CTG Cuts at: 4409 5336 5538 6121 4409 Size: 927 202 583 7283 Fragments arranged by size: 7283 927 583 202 SalI G'TCGA_C Cuts at: 1936 6337 1936 Size: 4401 4594 SanDI GG'GwC_CC Cuts at: 2742 2742 Size: 8995 SapI GCTCTTCn'nnn_ Cuts at: 4069 4069 Size: 8995 SbfI CC_TGCA'GG Cuts at: 6347 6347 Size: 8995 ScaI AGT'ACT Cuts at: 4531 5567 7243 8955 4531 Size: 1036 1676 1712 4571 Fragments arranged by size: 4571 1712 1676 1036 SexAI A'CCwGG_T Cuts at: 3805 3805 Size: 8995 SfcI C'TryA_G Cuts at: 826 1045 2289 5292 5487 6047 6111 6343 7478 Size: 219 1244 3003 195 560 64 232 1135 Cuts at: 7478 8159 8350 826 Size: 681 191 1471 Fragments arranged by size: 3003 1471 1244 1135 681 560 232 219 195 191 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8995 SmaI CCC'GGG Cuts at: 1872 6322 1872 Size: 4450 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2124 4356 4583 7099 8000 8262 Size: 197 926 181 2232 227 2516 901 262 Cuts at: 8262 820 Size: 1553 Fragments arranged by size: 2516 2232 1553 926 901 262 227 197 181 SnaBI TAC'GTA Cuts at: 493 6292 6786 493 Size: 5799 494 2702 Fragments arranged by size: 5799 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8995 SphI G_CATG'C Cuts at: 1132 6353 1132 Size: 5221 3774 SspI AAT'ATT Cuts at: 5 52 6211 6273 6919 5 Size: 47 6159 62 646 2081 Fragments arranged by size: 6159 2081 646 62 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8995 StyI C'CwwG_G Cuts at: 513 1065 3286 3616 3966 4053 4105 4176 4669 Size: 552 2221 330 350 87 52 71 493 Cuts at: 4669 4765 6434 513 Size: 96 1669 3074 Fragments arranged by size: 3074 2221 1669 552 493 350 330 96 87 71 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3259 5389 7022 7039 7192 7377 7822 Size: 1255 1679 2130 1633 17 153 185 445 Cuts at: 7822 325 Size: 1498 Fragments arranged by size: 2130 1679 1633 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3569 4529 5565 Size: 274 80 33 51 1443 1592 960 1036 Cuts at: 5565 7241 8953 96 Size: 1676 1712 138 Fragments arranged by size: 1712 1676 1592 1443 1036 960 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8955 8987 8955 Size: 32 8963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8995 XbaI T'CTAG_A Cuts at: 2567 6331 2567 Size: 3764 5231 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4660 4660 Size: 8995 XhoI C'TCGA_G Cuts at: 1943 2124 1943 Size: 181 8814 XmaI C'CCGG_G Cuts at: 1870 6320 1870 Size: 4450 4545 XmnI GAAnn'nnTTC Cuts at: 3173 3625 7124 3173 Size: 452 3499 5044 Fragments arranged by size: 5044 3499 452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6803 276 Size: 53 83 186 936 5269 2468 Fragments arranged by size: 5269 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SfiI SfoI SrfI SwaI