(Circular) (Six-base) MAPSORT of: pFN21ASDA1194.seq Check: 3672 from: 1 to: 6400 pFN21ASDA1194 6400 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4210 278 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2066 3743 1937 Size: 129 1677 4594 Fragments arranged by size: 4594 1677 129 Acc65I G'GTAC_C Cuts at: 3721 3721 Size: 6400 AclI AA'CG_TT Cuts at: 1563 4527 4900 1563 Size: 2964 373 3063 Fragments arranged by size: 3063 2964 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2208 2720 2794 2982 3352 4463 5847 6115 Size: 576 512 74 188 370 1111 1384 268 Cuts at: 6115 1632 Size: 1917 Fragments arranged by size: 1917 1384 1111 576 512 370 268 188 74 AflII C'TTAA_G Cuts at: 820 1017 2149 820 Size: 197 1132 5071 Fragments arranged by size: 5071 1132 197 AflIII A'CryG_T Cuts at: 1203 2857 5299 6276 1203 Size: 1654 2442 977 1327 Fragments arranged by size: 2442 1654 1327 977 AgeI A'CCGG_T Cuts at: 5231 5231 Size: 6400 AhdI GACnn_n'nnGTC Cuts at: 5129 5129 Size: 6400 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6400 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6400 AlwNI CAG_nnn'CTG Cuts at: 2501 3278 3585 4143 5715 2501 Size: 777 307 558 1572 3186 Fragments arranged by size: 3186 1572 777 558 307 ApaI G_GGCC'C Cuts at: 3545 3545 Size: 6400 ApaLI G'TGCA_C Cuts at: 4457 5613 4457 Size: 1156 5244 ApoI r'AATT_y Cuts at: 1493 2217 2277 2894 2924 2947 3224 3709 3935 Size: 724 60 617 30 23 277 485 226 Cuts at: 3935 4087 6196 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 724 617 485 277 226 152 60 30 23 AseI AT'TA_AT Cuts at: 160 1032 4954 160 Size: 872 3922 1606 Fragments arranged by size: 3922 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 AvaI C'yCGr_G Cuts at: 1870 1943 3725 1870 Size: 73 1782 4545 Fragments arranged by size: 4545 1782 73 BamHI G'GATC_C Cuts at: 3730 4097 3730 Size: 367 6033 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3721 5176 618 Size: 325 217 627 1934 1455 1842 Fragments arranged by size: 1934 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2328 3545 3642 3719 1391 Size: 655 282 1217 97 77 4072 Fragments arranged by size: 4072 1217 655 282 97 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2070 2726 928 Size: 971 171 656 4602 Fragments arranged by size: 4602 971 656 171 BbvCI CC'TCA_GC Cuts at: 3516 3516 Size: 6400 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3025 3059 4591 4625 1903 Size: 34 1088 34 1532 34 3678 Fragments arranged by size: 3678 1532 1088 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4292 5502 4292 Size: 1210 5190 BclI T'GATC_A Cuts at: 1548 1548 Size: 6400 BfrBI ATG'CAT Cuts at: 3109 3109 Size: 6400 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5011 136 Size: 107 122 71 631 605 3339 1525 Fragments arranged by size: 3339 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3256 6395 3256 Size: 3139 3261 BlpI GC'TnA_GC Cuts at: 3817 3817 Size: 6400 Bme1580I G_kGCm'C Cuts at: 1790 3545 4461 5617 1790 Size: 1755 916 1156 2573 Fragments arranged by size: 2573 1755 1156 916 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6400 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2122 2680 4131 Size: 416 53 83 399 24 705 558 1451 Cuts at: 4131 5089 442 Size: 958 1753 Fragments arranged by size: 1753 1451 958 705 558 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2939 1056 Size: 1883 4517 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3656 5060 1239 Size: 2417 1404 2579 Fragments arranged by size: 2579 2417 1404 Bpu10I CC'TnA_GC Cuts at: 3516 3516 Size: 6400 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2728 4525 5390 5688 2728 Size: 1797 865 298 3440 Fragments arranged by size: 3440 1797 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5063 882 Size: 617 3564 2219 Fragments arranged by size: 3564 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3697 4191 493 Size: 711 2493 494 2702 Fragments arranged by size: 2702 2493 711 494 BsaBI GATnn'nnATC Cuts at: 6394 6394 Size: 6400 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4207 4589 5224 275 Size: 53 83 186 936 2674 382 635 1451 Fragments arranged by size: 2674 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4833 5231 5505 5652 6063 4833 Size: 398 274 147 411 5170 Fragments arranged by size: 5170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3329 3359 1178 Size: 30 165 30 1926 30 4219 Fragments arranged by size: 4219 1926 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6400 BseYI C'CCAG_C Cuts at: 2368 5603 6099 2368 Size: 3235 496 2669 Fragments arranged by size: 3235 2669 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3273 1163 Size: 573 1537 4290 Fragments arranged by size: 4290 1537 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3850 4611 5639 1540 Size: 275 186 1849 761 1028 2301 Fragments arranged by size: 2301 1849 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 3302 3642 4461 4546 5617 2328 Size: 974 340 819 85 1071 3111 Fragments arranged by size: 3111 1071 974 819 340 85 BsmI GAATG_Cn' Cuts at: 3911 4004 3911 Size: 93 6307 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2328 3302 3545 3642 3719 4461 Size: 399 256 282 974 243 97 77 742 Cuts at: 4461 4546 5617 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 974 742 399 282 256 243 97 85 77 BspEI T'CCGG_A Cuts at: 6063 6063 Size: 6400 BspHI T'CATG_A Cuts at: 2982 4289 6019 2982 Size: 1307 1730 3363 Fragments arranged by size: 3363 1730 1307 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3755 844 Size: 2911 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4287 1394 Size: 429 2464 3507 Fragments arranged by size: 3507 2464 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2229 2722 4895 5069 65 Size: 1183 981 493 2173 174 1396 Fragments arranged by size: 2173 1396 1183 981 493 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5044 5231 1700 Size: 110 3234 187 2869 Fragments arranged by size: 3234 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6400 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6400 BssSI C'ACGA_G Cuts at: 4460 5472 4460 Size: 1012 5388 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6400 BstBI TT'CG_AA Cuts at: 2007 5204 2007 Size: 3197 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6400 BstXI CCAn_nnnn'nTGG Cuts at: 2541 3217 3683 2541 Size: 676 466 5258 Fragments arranged by size: 5258 676 466 BstYI r'GATC_y Cuts at: 1972 3256 3730 4097 4482 4499 5951 6037 6049 Size: 1284 474 367 385 17 1452 86 12 Cuts at: 6049 6395 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1284 474 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2067 2067 Size: 6400 Bsu36I CC'TnA_GG Cuts at: 3529 3529 Size: 6400 BtgI C'CryG_G Cuts at: 513 1065 2940 513 Size: 552 1875 3973 Fragments arranged by size: 3973 1875 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5661 Fragments arranged by size: 5661 683 56 BtsI GCAGTG_nn' Cuts at: 3916 4710 4730 3916 Size: 794 20 5586 Fragments arranged by size: 5586 794 20 ClaI AT'CG_AT Cuts at: 1554 3035 1554 Size: 1481 4919 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3092 3127 529 Size: 35 2528 35 3802 Fragments arranged by size: 3802 2528 35 35 DraI TTT'AAA Cuts at: 2334 3704 2334 Size: 1370 5030 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5407 809 Size: 507 4091 1802 Fragments arranged by size: 4091 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3847 4736 6365 Size: 54 1425 50 275 18 2017 889 1629 Cuts at: 6365 8 Size: 43 Fragments arranged by size: 2017 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3847 1537 Size: 275 2035 4090 Fragments arranged by size: 4090 2035 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4330 1353 Size: 2977 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4987 5361 5507 387 Size: 1064 138 3398 374 146 1280 Fragments arranged by size: 3398 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2326 3640 2326 Size: 1314 5086 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 2720 2794 2982 3352 3656 4463 Size: 393 576 512 74 188 370 304 807 Cuts at: 4463 5060 5847 6115 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 576 512 393 370 304 268 188 74 EcoNI CCTnn'n_nnAGG Cuts at: 2420 2441 2420 Size: 21 6379 EcoO109I rG'GnC_Cy Cuts at: 3115 3542 3115 Size: 427 5973 EcoRI G'AATT_C Cuts at: 2217 3709 4087 2217 Size: 1492 378 4530 Fragments arranged by size: 4530 1492 378 EcoRV GAT'ATC Cuts at: 2725 2725 Size: 6400 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2175 2175 Size: 6400 FspI TGC'GCA Cuts at: 4906 4906 Size: 6400 HaeII r_GCGC'y Cuts at: 1678 2666 5547 1678 Size: 988 2881 2531 Fragments arranged by size: 2881 2531 988 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2284 2477 2509 3176 Size: 32 135 32 680 32 193 32 667 Cuts at: 3176 3208 3602 3634 5041 5073 5115 5147 1373 Size: 32 394 32 1407 32 42 32 2626 Fragments arranged by size: 2626 1407 680 667 394 193 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3667 3744 3990 669 Size: 988 281 1729 77 246 3079 Fragments arranged by size: 3079 1729 988 281 246 77 HindIII A'AGCT_T Cuts at: 748 2146 2237 748 Size: 1398 91 4911 Fragments arranged by size: 4911 1398 91 HpaI GTT'AAC Cuts at: 3990 3990 Size: 6400 KpnI G_GTAC'C Cuts at: 3725 3725 Size: 6400 MfeI C'AATT_G Cuts at: 3999 3999 Size: 6400 MluI A'CGCG_T Cuts at: 6276 6276 Size: 6400 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5514 5698 1305 Size: 4209 184 2007 Fragments arranged by size: 4209 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4578 Fragments arranged by size: 4578 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2161 2196 2862 4358 4717 4876 518 Size: 648 995 35 666 1496 359 159 2042 Fragments arranged by size: 2042 1496 995 666 648 359 159 35 MspA1I CmG'CkG Cuts at: 1606 3372 3520 3816 4493 5641 5886 1606 Size: 1766 148 296 677 1148 245 2120 Fragments arranged by size: 2120 1766 1148 677 296 245 148 NcoI C'CATG_G Cuts at: 513 1065 2940 513 Size: 552 1875 3973 Fragments arranged by size: 3973 1875 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6400 NheI G'CTAG_C Cuts at: 1052 2935 1052 Size: 1883 4517 NotI GC'GGCC_GC Cuts at: 3847 3847 Size: 6400 NsiI A_TGCA'T Cuts at: 3111 3111 Size: 6400 NspI r_CATG'y Cuts at: 1132 1229 2861 3109 3758 5303 1132 Size: 97 1632 248 649 1545 2229 Fragments arranged by size: 2229 1632 1545 649 248 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6400 PciI A'CATG_T Cuts at: 2857 5299 2857 Size: 2442 3958 PflMI CCAn_nnn'nTGG Cuts at: 1108 3065 3278 3562 1108 Size: 1957 213 284 3946 Fragments arranged by size: 3946 1957 284 213 PmeI GTTT'AAAC Cuts at: 3704 3704 Size: 6400 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4469 4501 5982 6014 4469 Size: 32 1481 32 4855 Fragments arranged by size: 4855 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3115 3115 Size: 6400 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6400 PsiI TTA'TAA Cuts at: 3970 3970 Size: 6400 PspOMI G'GGCC_C Cuts at: 3541 3541 Size: 6400 PstI C_TGCA'G Cuts at: 830 3752 830 Size: 2922 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6400 PvuII CAG'CTG Cuts at: 3372 3520 3372 Size: 148 6252 SacI G_AGCT'C Cuts at: 2328 3642 2328 Size: 1314 5086 SalI G'TCGA_C Cuts at: 1936 3742 1936 Size: 1806 4594 SanDI GG'GwC_CC Cuts at: 3115 3115 Size: 6400 SbfI CC_TGCA'GG Cuts at: 3752 3752 Size: 6400 ScaI AGT'ACT Cuts at: 3012 4648 6360 3012 Size: 1636 1712 3052 Fragments arranged by size: 3052 1712 1636 SfcI C'TryA_G Cuts at: 826 1045 2677 3748 4883 5564 5755 826 Size: 219 1632 1071 1135 681 191 1471 Fragments arranged by size: 1632 1471 1135 1071 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6400 SmaI CCC'GGG Cuts at: 1872 3727 1872 Size: 1855 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2149 2707 4504 5405 5667 820 Size: 197 926 206 558 1797 901 262 1553 Fragments arranged by size: 1797 1553 926 901 558 262 206 197 SnaBI TAC'GTA Cuts at: 493 3697 4191 493 Size: 3204 494 2702 Fragments arranged by size: 3204 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6400 SphI G_CATG'C Cuts at: 1132 3758 1132 Size: 2626 3774 SspI AAT'ATT Cuts at: 5 52 2877 3293 4324 5 Size: 47 2825 416 1031 2081 Fragments arranged by size: 2825 2081 1031 416 47 StuI AGG'CCT Cuts at: 1517 2400 1517 Size: 883 5517 StyI C'CwwG_G Cuts at: 513 1065 2534 2940 3839 513 Size: 552 1469 406 899 3074 Fragments arranged by size: 3074 1469 899 552 406 SwaI ATTT'AAAT Cuts at: 2334 2334 Size: 6400 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4427 4444 4597 4782 5227 325 Size: 1255 2847 17 153 185 445 1498 Fragments arranged by size: 2847 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3010 4646 6358 Size: 274 80 33 51 1443 1033 1636 1712 Cuts at: 6358 96 Size: 138 Fragments arranged by size: 1712 1636 1443 1033 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6360 6392 6360 Size: 32 6368 Tth111I GACn'n_nGTC Cuts at: 1316 2836 1316 Size: 1520 4880 XbaI T'CTAG_A Cuts at: 2822 3736 2822 Size: 914 5486 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6400 XmaI C'CCGG_G Cuts at: 1870 3725 1870 Size: 1855 4545 XmnI GAAnn'nnTTC Cuts at: 2221 4529 2221 Size: 2308 4092 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4208 276 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI XcmI