(Circular) (Six-base) MAPSORT of: pFN21ASDA1186.seq Check: 988 from: 1 to: 5938 pFN21ASDA1186 5938 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3092 3092 Size: 5938 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3748 278 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3281 1937 Size: 1344 4594 Acc65I G'GTAC_C Cuts at: 3259 3259 Size: 5938 AclI AA'CG_TT Cuts at: 1563 4065 4438 1563 Size: 2502 373 3063 Fragments arranged by size: 3063 2502 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 3002 3087 4001 5385 5653 1632 Size: 968 402 85 914 1384 268 1917 Fragments arranged by size: 1917 1384 968 914 402 268 85 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5741 AflIII A'CryG_T Cuts at: 1203 4837 5814 1203 Size: 3634 977 1327 Fragments arranged by size: 3634 1327 977 AgeI A'CCGG_T Cuts at: 4769 4769 Size: 5938 AhdI GACnn_n'nnGTC Cuts at: 4667 4667 Size: 5938 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5938 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2442 1184 Size: 1258 4680 AlwNI CAG_nnn'CTG Cuts at: 2272 2380 3681 5253 2272 Size: 108 1301 1572 2957 Fragments arranged by size: 2957 1572 1301 108 ApaLI G'TGCA_C Cuts at: 3995 5151 3995 Size: 1156 4782 ApoI r'AATT_y Cuts at: 1493 3247 3473 3625 5734 1493 Size: 1754 226 152 2109 1697 Fragments arranged by size: 2109 1754 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4492 160 Size: 872 3460 1606 Fragments arranged by size: 3460 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 AvaI C'yCGr_G Cuts at: 1870 1943 3263 1870 Size: 73 1320 4545 Fragments arranged by size: 4545 1320 73 BamHI G'GATC_C Cuts at: 3268 3635 3268 Size: 367 5571 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2731 2750 3221 3259 4714 Size: 325 217 627 944 19 471 38 1455 Cuts at: 4714 618 Size: 1842 Fragments arranged by size: 1842 1455 944 627 471 325 217 38 19 BanII G_rGCy'C Cuts at: 1391 2051 3257 1391 Size: 660 1206 4072 Fragments arranged by size: 4072 1206 660 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2990 928 Size: 971 1091 3876 Fragments arranged by size: 3876 1091 971 BbvCI CC'TCA_GC Cuts at: 2384 2384 Size: 5938 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4163 1903 Size: 34 2192 34 3678 Fragments arranged by size: 3678 2192 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3830 5040 3830 Size: 1210 4728 BclI T'GATC_A Cuts at: 1548 1548 Size: 5938 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4549 136 Size: 107 122 71 631 605 2877 1525 Fragments arranged by size: 2877 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5933 5933 Size: 5938 BlpI GC'TnA_GC Cuts at: 3355 3355 Size: 5938 Bme1580I G_kGCm'C Cuts at: 1790 2431 2753 3999 5155 1790 Size: 641 322 1246 1156 2573 Fragments arranged by size: 2573 1246 1156 641 322 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5938 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2306 3669 4627 Size: 416 53 83 399 24 889 1363 958 Cuts at: 4627 442 Size: 1753 Fragments arranged by size: 1753 1363 958 889 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5938 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2561 2561 Size: 5938 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2235 4598 1239 Size: 996 2363 2579 Fragments arranged by size: 2579 2363 996 Bpu10I CC'TnA_GC Cuts at: 2384 2384 Size: 5938 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2486 4063 4928 5226 2486 Size: 1577 865 298 3198 Fragments arranged by size: 3198 1577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2456 2829 4601 882 Size: 617 957 373 1772 2219 Fragments arranged by size: 2219 1772 957 617 373 BsaAI yAC'GTr Cuts at: 493 1204 3235 3729 493 Size: 711 2031 494 2702 Fragments arranged by size: 2702 2031 711 494 BsaBI GATnn'nnATC Cuts at: 5932 5932 Size: 5938 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3745 4127 4762 275 Size: 53 83 186 936 2212 382 635 1451 Fragments arranged by size: 2212 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2190 4371 4769 5043 5190 5601 2190 Size: 2181 398 274 147 411 2527 Fragments arranged by size: 2527 2181 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2946 2976 1178 Size: 30 165 30 1543 30 4140 Fragments arranged by size: 4140 1543 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2875 1186 Size: 1689 4249 BseYI C'CCAG_C Cuts at: 3211 5141 5637 3211 Size: 1930 496 3512 Fragments arranged by size: 3512 1930 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2888 1163 Size: 573 1152 4213 Fragments arranged by size: 4213 1152 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3388 4149 5177 1540 Size: 275 186 1387 761 1028 2301 Fragments arranged by size: 2301 1387 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3999 4084 5155 3999 Size: 85 1071 4782 Fragments arranged by size: 4782 1071 85 BsmI GAATG_Cn' Cuts at: 3449 3542 3449 Size: 93 5845 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2431 2753 3257 3999 4084 5155 Size: 399 261 380 322 504 742 85 1071 Cuts at: 5155 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 504 399 380 322 261 85 BspEI T'CCGG_A Cuts at: 2190 5601 2190 Size: 3411 2527 BspHI T'CATG_A Cuts at: 3827 5557 3827 Size: 1730 4208 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 2388 3092 3293 844 Size: 1331 213 704 201 3489 Fragments arranged by size: 3489 1331 704 213 201 BsrBI CCG'CTC Cuts at: 1394 1823 2028 2957 3825 1394 Size: 429 205 929 868 3507 Fragments arranged by size: 3507 929 868 429 205 BsrDI GCAATG_nn' Cuts at: 65 1248 4433 4607 65 Size: 1183 3185 174 1396 Fragments arranged by size: 3185 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2104 4582 4769 1700 Size: 110 294 2478 187 2869 Fragments arranged by size: 2869 2478 294 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5938 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5938 BssSI C'ACGA_G Cuts at: 3998 5010 3998 Size: 1012 4926 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5938 BstBI TT'CG_AA Cuts at: 2007 4742 2007 Size: 2735 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5938 BstXI CCAn_nnnn'nTGG Cuts at: 2446 2446 Size: 5938 BstYI r'GATC_y Cuts at: 1972 2907 3268 3635 4020 4037 5489 5575 5587 Size: 935 361 367 385 17 1452 86 12 Cuts at: 5587 5933 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 935 385 367 361 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2930 2930 Size: 5938 BtgI C'CryG_G Cuts at: 513 1065 2878 513 Size: 552 1813 3573 Fragments arranged by size: 3573 1813 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5199 Fragments arranged by size: 5199 683 56 BtsI GCAGTG_nn' Cuts at: 3094 3454 4248 4268 3094 Size: 360 794 20 4764 Fragments arranged by size: 4764 794 360 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5903 DraI TTT'AAA Cuts at: 3242 3242 Size: 5938 DraIII CAC_nnn'GTG Cuts at: 2866 2896 2866 Size: 30 5908 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4945 809 Size: 507 3629 1802 Fragments arranged by size: 3629 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 3385 4274 Size: 54 1425 50 275 18 240 1315 889 Cuts at: 4274 5903 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1315 889 275 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3385 1537 Size: 275 1573 4090 Fragments arranged by size: 4090 1573 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2918 3189 3868 1353 Size: 1565 271 679 3423 Fragments arranged by size: 3423 1565 679 271 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4525 4899 5045 387 Size: 1064 138 2936 374 146 1280 Fragments arranged by size: 2936 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2235 2600 3002 3087 4001 4598 5385 Size: 393 603 365 402 85 914 597 787 Cuts at: 5385 5653 1239 Size: 268 1524 Fragments arranged by size: 1524 914 787 603 597 402 393 365 268 85 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2651 Size: 5938 EcoO109I rG'GnC_Cy Cuts at: 2377 3075 3169 2377 Size: 698 94 5146 Fragments arranged by size: 5146 698 94 EcoRI G'AATT_C Cuts at: 3247 3625 3247 Size: 378 5560 FspI TGC'GCA Cuts at: 4444 4444 Size: 5938 HaeII r_GCGC'y Cuts at: 1678 5085 1678 Size: 3407 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3173 3205 4579 4611 4653 Size: 32 135 32 1601 32 1374 32 42 Cuts at: 4653 4685 1373 Size: 32 2626 Fragments arranged by size: 2626 1601 1374 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3282 3528 669 Size: 988 281 1344 246 3079 Fragments arranged by size: 3079 1344 988 281 246 HindIII A'AGCT_T Cuts at: 748 2158 748 Size: 1410 4528 HpaI GTT'AAC Cuts at: 3528 3528 Size: 5938 KpnI G_GTAC'C Cuts at: 3263 3263 Size: 5938 MfeI C'AATT_G Cuts at: 3537 3537 Size: 5938 MluI A'CGCG_T Cuts at: 5814 5814 Size: 5938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2157 2287 5052 5236 1305 Size: 852 130 2765 184 2007 Fragments arranged by size: 2765 2007 852 184 130 MscI TGG'CCA Cuts at: 10 64 1489 1832 2072 10 Size: 54 1425 343 240 3876 Fragments arranged by size: 3876 1425 343 240 54 MslI CAynn'nnrTG Cuts at: 518 1166 3896 4255 4414 518 Size: 648 2730 359 159 2042 Fragments arranged by size: 2730 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3007 3211 3354 4031 5179 5424 1606 Size: 1401 204 143 677 1148 245 2120 Fragments arranged by size: 2120 1401 1148 677 245 204 143 NaeI GCC'GGC Cuts at: 2106 2106 Size: 5938 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5386 NdeI CA'TA_TG Cuts at: 387 387 Size: 5938 NgoMIV G'CCGG_C Cuts at: 2104 2104 Size: 5938 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5938 NotI GC'GGCC_GC Cuts at: 3385 3385 Size: 5938 NspI r_CATG'y Cuts at: 1132 1229 2201 2554 3296 4841 1132 Size: 97 972 353 742 1545 2229 Fragments arranged by size: 2229 1545 972 742 353 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5938 PciI A'CATG_T Cuts at: 4837 4837 Size: 5938 PflMI CCAn_nnn'nTGG Cuts at: 1108 2896 1108 Size: 1788 4150 PfoI T'CCnGG_A Cuts at: 2091 2091 Size: 5938 PmeI GTTT'AAAC Cuts at: 3242 3242 Size: 5938 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4007 4039 5520 5552 4007 Size: 32 1481 32 4393 Fragments arranged by size: 4393 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2377 3075 3169 2377 Size: 698 94 5146 Fragments arranged by size: 5146 698 94 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5938 PsiI TTA'TAA Cuts at: 3508 3508 Size: 5938 PstI C_TGCA'G Cuts at: 830 2172 3089 3290 830 Size: 1342 917 201 3478 Fragments arranged by size: 3478 1342 917 201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5938 PvuII CAG'CTG Cuts at: 3211 3211 Size: 5938 SalI G'TCGA_C Cuts at: 1936 3280 1936 Size: 1344 4594 SanDI GG'GwC_CC Cuts at: 3075 3075 Size: 5938 SbfI CC_TGCA'GG Cuts at: 3290 3290 Size: 5938 ScaI AGT'ACT Cuts at: 4186 5898 4186 Size: 1712 4226 SexAI A'CCwGG_T Cuts at: 2727 2838 3057 2727 Size: 111 219 5608 Fragments arranged by size: 5608 219 111 SfcI C'TryA_G Cuts at: 826 1045 2168 3085 3127 3286 4421 5102 5293 Size: 219 1123 917 42 159 1135 681 191 Cuts at: 5293 826 Size: 1471 Fragments arranged by size: 1471 1135 1123 917 681 219 191 159 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5938 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5938 SmaI CCC'GGG Cuts at: 1872 3265 1872 Size: 1393 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2501 4042 4943 5205 820 Size: 197 926 558 1541 901 262 1553 Fragments arranged by size: 1553 1541 926 901 558 262 197 SnaBI TAC'GTA Cuts at: 493 3235 3729 493 Size: 2742 494 2702 Fragments arranged by size: 2742 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5938 SphI G_CATG'C Cuts at: 1132 3296 1132 Size: 2164 3774 SspI AAT'ATT Cuts at: 5 52 3862 5 Size: 47 3810 2081 Fragments arranged by size: 3810 2081 47 StuI AGG'CCT Cuts at: 1517 2531 1517 Size: 1014 4924 StyI C'CwwG_G Cuts at: 513 1065 2346 3377 513 Size: 552 1281 1031 3074 Fragments arranged by size: 3074 1281 1031 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3965 3982 4135 4320 4765 325 Size: 1255 2385 17 153 185 445 1498 Fragments arranged by size: 2385 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4184 5896 96 Size: 274 80 33 51 1443 2207 1712 138 Fragments arranged by size: 2207 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5898 5930 5898 Size: 32 5906 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5938 XbaI T'CTAG_A Cuts at: 3274 3274 Size: 5938 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5938 XmaI C'CCGG_G Cuts at: 1870 3263 1870 Size: 1393 4545 XmnI GAAnn'nnTTC Cuts at: 4067 4067 Size: 5938 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3746 276 Size: 53 83 186 936 2212 2468 Fragments arranged by size: 2468 2212 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI