(Circular) (Six-base) MAPSORT of: pFN21ASDA1152.seq Check: 3687 from: 1 to: 5782 pFN21ASDA1152 5782 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2392 2950 3125 1937 Size: 455 558 175 4594 Fragments arranged by size: 4594 558 455 175 Acc65I G'GTAC_C Cuts at: 2928 3103 2928 Size: 175 5607 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2435 2483 2868 3845 5229 5497 1632 Size: 803 48 385 977 1384 268 1917 Fragments arranged by size: 1917 1384 977 803 385 268 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 2673 4681 5658 1203 Size: 1470 2008 977 1327 Fragments arranged by size: 2008 1470 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2986 1184 Size: 1802 3980 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2149 2181 2149 Size: 32 5750 AlwNI CAG_nnn'CTG Cuts at: 2461 2524 3525 5097 2461 Size: 63 1001 1572 3146 Fragments arranged by size: 3146 1572 1001 63 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 2879 3091 3317 3469 5578 1493 Size: 1386 212 226 152 2109 1697 Fragments arranged by size: 2109 1697 1386 226 212 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 3107 1870 Size: 73 1164 4545 Fragments arranged by size: 4545 1164 73 BamHI G'GATC_C Cuts at: 3112 3479 3112 Size: 367 5415 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2928 3103 4558 618 Size: 325 217 627 1141 175 1455 1842 Fragments arranged by size: 1842 1455 1141 627 325 217 175 BanII G_rGCy'C Cuts at: 1391 2117 2663 2724 2833 3101 1391 Size: 726 546 61 109 268 4072 Fragments arranged by size: 4072 726 546 268 109 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BbvCI CC'TCA_GC Cuts at: 2050 2050 Size: 5782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3079 3674 4884 3079 Size: 595 1210 3977 Fragments arranged by size: 3977 1210 595 BclI T'GATC_A Cuts at: 1548 2836 1548 Size: 1288 4494 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4393 136 Size: 107 122 71 631 605 2721 1525 Fragments arranged by size: 2721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 2111 3199 2111 Size: 1088 4694 Bme1580I G_kGCm'C Cuts at: 1790 3843 4999 1790 Size: 2053 1156 2573 Fragments arranged by size: 2573 2053 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2727 3513 4471 Size: 416 53 83 399 24 1310 786 958 Cuts at: 4471 442 Size: 1753 Fragments arranged by size: 1753 1310 958 786 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2448 1056 Size: 1392 4390 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2976 4442 1239 Size: 1737 1466 2579 Fragments arranged by size: 2579 1737 1466 Bpu10I CC'TnA_GC Cuts at: 2050 2603 2050 Size: 553 5229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4445 882 Size: 617 2946 2219 Fragments arranged by size: 2946 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3079 3573 493 Size: 711 1875 494 2702 Fragments arranged by size: 2702 1875 711 494 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2263 4215 4613 4887 5034 5445 2263 Size: 1952 398 274 147 411 2600 Fragments arranged by size: 2600 1952 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2775 2805 1178 Size: 30 165 30 1372 30 4155 Fragments arranged by size: 4155 1372 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 1186 Size: 928 4854 BseYI C'CCAG_C Cuts at: 2314 4985 5481 2314 Size: 2671 496 2615 Fragments arranged by size: 2671 2615 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3843 3928 4999 3843 Size: 85 1071 4626 Fragments arranged by size: 4626 1071 85 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2663 2724 2833 3101 3843 3928 Size: 399 327 546 61 109 268 742 85 Cuts at: 3928 4999 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 546 399 327 268 109 85 61 BspEI T'CCGG_A Cuts at: 5445 5445 Size: 5782 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2204 2487 3137 844 Size: 1360 283 650 3489 Fragments arranged by size: 3489 1360 650 283 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 2007 4586 2007 Size: 2579 3203 BstEII G'GTnAC_C Cuts at: 1184 2266 1184 Size: 1082 4700 BstYI r'GATC_y Cuts at: 1972 3112 3479 3864 3881 5333 5419 5431 5777 Size: 1140 367 385 17 1452 86 12 346 Cuts at: 5777 1972 Size: 1977 Fragments arranged by size: 1977 1452 1140 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2566 513 Size: 552 957 544 3729 Fragments arranged by size: 3729 957 552 544 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 2701 2716 3298 4092 4112 2701 Size: 15 582 794 20 4371 Fragments arranged by size: 4371 794 582 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2912 3229 4118 Size: 54 1425 50 275 18 1082 317 889 Cuts at: 4118 5747 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1082 889 317 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3712 1353 Size: 2359 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2425 4369 4743 4889 387 Size: 1064 138 836 1944 374 146 1280 Fragments arranged by size: 1944 1280 1064 836 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2435 2483 2868 2976 3845 4442 5229 Size: 393 803 48 385 108 869 597 787 Cuts at: 5229 5497 1239 Size: 268 1524 Fragments arranged by size: 1524 869 803 787 597 393 385 268 108 48 EcoNI CCTnn'n_nnAGG Cuts at: 2978 2978 Size: 5782 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2111 4929 1678 Size: 433 2818 2531 Fragments arranged by size: 2818 2531 433 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2051 2065 2083 2660 Size: 32 135 32 461 18 14 18 577 Cuts at: 2660 2692 2775 2790 2807 2822 4423 4455 4497 Size: 32 83 15 17 15 1601 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 1601 577 461 135 83 42 32 32 32 32 32 18 18 17 15 15 14 HincII GTy'rAC Cuts at: 669 1657 1938 2393 3126 3372 669 Size: 988 281 455 733 246 3079 Fragments arranged by size: 3079 988 733 455 281 246 HindIII A'AGCT_T Cuts at: 748 2081 748 Size: 1333 4449 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 2932 3107 2932 Size: 175 5607 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2135 4896 5080 1305 Size: 830 2761 184 2007 Fragments arranged by size: 2761 2007 830 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3740 4099 4258 518 Size: 648 2574 359 159 2042 Fragments arranged by size: 2574 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2532 3198 3875 5023 5268 1606 Size: 926 666 677 1148 245 2120 Fragments arranged by size: 2120 1148 926 677 666 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4273 Fragments arranged by size: 4273 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 2444 1052 Size: 1392 4390 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2677 3140 4685 1132 Size: 97 1448 463 1545 2229 Fragments arranged by size: 2229 1545 1448 463 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 2673 4681 2673 Size: 2008 3774 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2498 2965 3134 830 Size: 1668 467 169 3478 Fragments arranged by size: 3478 1668 467 169 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 SalI G'TCGA_C Cuts at: 1936 2391 3124 1936 Size: 455 733 4594 Fragments arranged by size: 4594 733 455 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2203 2494 2961 3130 4265 4946 5137 Size: 219 1158 291 467 169 1135 681 191 Cuts at: 5137 826 Size: 1471 Fragments arranged by size: 1471 1158 1135 681 467 291 219 191 169 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3079 3573 493 Size: 2586 494 2702 Fragments arranged by size: 2702 2586 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3049 3706 5 Size: 47 2997 657 2081 Fragments arranged by size: 2997 2081 657 47 StuI AGG'CCT Cuts at: 1517 2213 1517 Size: 696 5086 StyI C'CwwG_G Cuts at: 513 1065 2022 2055 3221 513 Size: 552 957 33 1166 3074 Fragments arranged by size: 3074 1166 957 552 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2328 3809 3826 3979 4164 4609 325 Size: 1255 748 1481 17 153 185 445 1498 Fragments arranged by size: 1498 1481 1255 748 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4028 5740 96 Size: 274 80 33 51 1443 2051 1712 138 Fragments arranged by size: 2051 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2620 2652 5742 5774 2620 Size: 32 3090 32 2628 Fragments arranged by size: 3090 2628 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 2863 3911 2863 Size: 1048 4734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI