(Circular) (Six-base) MAPSORT of: pFN21ASDA1138.seq Check: 9421 from: 1 to: 8392 pFN21ASDA1138 8392 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2839 3184 2839 Size: 345 8047 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5397 6202 278 Size: 53 83 186 936 3861 805 2468 Fragments arranged by size: 3861 2468 936 805 186 83 53 AccI GT'mk_AC Cuts at: 1937 5268 5735 1937 Size: 3331 467 4594 Fragments arranged by size: 4594 3331 467 Acc65I G'GTAC_C Cuts at: 5713 5713 Size: 8392 AclI AA'CG_TT Cuts at: 1563 6519 6892 1563 Size: 4956 373 3063 Fragments arranged by size: 4956 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3575 4959 5495 6455 7839 8107 1632 Size: 1943 1384 536 960 1384 268 1917 Fragments arranged by size: 1943 1917 1384 1384 960 536 268 AfeI AGC'GCT Cuts at: 4481 4654 5425 4481 Size: 173 771 7448 Fragments arranged by size: 7448 771 173 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8195 AflIII A'CryG_T Cuts at: 1203 5064 5116 5502 7291 8268 1203 Size: 3861 52 386 1789 977 1327 Fragments arranged by size: 3861 1789 1327 977 386 52 AgeI A'CCGG_T Cuts at: 7223 7223 Size: 8392 AhdI GACnn_n'nnGTC Cuts at: 7121 7121 Size: 8392 AleI CACnn'nnGTG Cuts at: 1166 3347 5276 5300 5495 5507 1166 Size: 2181 1929 24 195 12 4051 Fragments arranged by size: 4051 2181 1929 195 24 12 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8392 AlwNI CAG_nnn'CTG Cuts at: 4330 4938 6135 7707 4330 Size: 608 1197 1572 5015 Fragments arranged by size: 5015 1572 1197 608 ApaI G_GGCC'C Cuts at: 2091 2131 2138 2441 3172 3202 3275 3568 4117 Size: 40 7 303 731 30 73 293 549 Cuts at: 4117 2091 Size: 6366 Fragments arranged by size: 6366 731 549 303 293 73 40 30 7 ApaLI G'TGCA_C Cuts at: 5279 6449 7605 5279 Size: 1170 1156 6066 Fragments arranged by size: 6066 1170 1156 ApoI r'AATT_y Cuts at: 1493 5701 5927 6079 8188 1493 Size: 4208 226 152 2109 1697 Fragments arranged by size: 4208 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2142 2142 Size: 8392 AseI AT'TA_AT Cuts at: 160 1032 6946 160 Size: 872 5914 1606 Fragments arranged by size: 5914 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8392 AvaI C'yCGr_G Cuts at: 1870 1943 2081 2130 2772 2799 3992 4048 4767 Size: 73 138 49 642 27 1193 56 719 Cuts at: 4767 4877 5194 5717 1870 Size: 110 317 523 4545 Fragments arranged by size: 4545 1193 719 642 523 317 138 110 73 56 49 27 BamHI G'GATC_C Cuts at: 5722 6089 5722 Size: 367 8025 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 2118 2164 2240 2404 Size: 325 217 627 290 41 46 76 164 Cuts at: 2404 2612 2751 3530 3625 4066 4262 4952 5630 Size: 208 139 779 95 441 196 690 678 Cuts at: 5630 5713 7168 618 Size: 83 1455 1842 Fragments arranged by size: 1842 1455 779 690 678 627 441 325 290 217 208 196 164 139 95 83 76 46 41 BanII G_rGCy'C Cuts at: 1391 2091 2131 2138 2441 2807 2823 2925 3172 Size: 700 40 7 303 366 16 102 247 Cuts at: 3172 3202 3275 3401 3568 4047 4117 5178 5711 Size: 30 73 126 167 479 70 1061 533 Cuts at: 5711 1391 Size: 4072 Fragments arranged by size: 4072 1061 700 533 479 366 303 247 167 126 102 73 70 40 30 16 7 BbeI G_GCGC'C Cuts at: 2081 2168 2408 3534 5634 2081 Size: 87 240 1126 2100 4839 Fragments arranged by size: 4839 2100 1126 240 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5165 5673 928 Size: 971 3266 508 3647 Fragments arranged by size: 3647 3266 971 508 BbvCI CC'TCA_GC Cuts at: 2756 3659 4667 4902 2756 Size: 903 1008 235 6246 Fragments arranged by size: 6246 1008 903 235 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4129 4158 4163 4192 6583 6617 1903 Size: 34 2192 29 5 29 2391 34 3678 Fragments arranged by size: 3678 2391 2192 34 34 29 29 5 BciVI GTATCCnnnnn_n' Cuts at: 6284 7494 6284 Size: 1210 7182 BclI T'GATC_A Cuts at: 1548 1548 Size: 8392 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2086 2095 2309 Size: 107 122 71 631 605 414 9 214 Cuts at: 2309 2653 3379 3388 3727 4121 4166 4950 5148 Size: 344 726 9 339 394 45 784 198 Cuts at: 5148 7003 136 Size: 1855 1525 Fragments arranged by size: 1855 1525 784 726 631 605 414 394 344 339 214 198 122 107 71 45 9 9 BglII A'GATC_T Cuts at: 8387 8387 Size: 8392 BlpI GC'TnA_GC Cuts at: 4748 5411 5809 4748 Size: 663 398 7331 Fragments arranged by size: 7331 663 398 Bme1580I G_kGCm'C Cuts at: 1790 2091 2131 2138 2441 2617 3172 3202 3275 Size: 301 40 7 303 176 555 30 73 Cuts at: 3275 3568 4071 4117 4662 5283 5610 6453 7609 Size: 293 503 46 545 621 327 843 1156 Cuts at: 7609 1790 Size: 2573 Fragments arranged by size: 2573 1156 843 621 555 545 503 327 303 301 293 176 73 46 40 30 7 BmgBI CAC'GTC Cuts at: 1318 3058 5278 5505 1318 Size: 1740 2220 227 4205 Fragments arranged by size: 4205 2220 1740 227 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2667 5023 5053 Size: 416 53 83 399 24 1250 2356 30 Cuts at: 5053 6123 7081 442 Size: 1070 958 1753 Fragments arranged by size: 2356 1753 1250 1070 958 416 399 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8392 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2398 3550 2398 Size: 1152 7240 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2354 2381 2683 2738 3023 3053 3437 Size: 1061 54 27 302 55 285 30 384 Cuts at: 3437 3673 3778 3848 5264 5474 5599 7052 1239 Size: 236 105 70 1416 210 125 1453 2579 Fragments arranged by size: 2579 1453 1416 1061 384 302 285 236 210 125 105 70 55 54 30 27 Bpu10I CC'TnA_GC Cuts at: 2205 2756 3569 3659 4311 4386 4634 4667 4902 Size: 551 813 90 652 75 248 33 235 Cuts at: 4902 2205 Size: 5695 Fragments arranged by size: 5695 813 652 551 248 235 90 75 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3716 4496 4610 4823 5165 6517 7382 7680 3716 Size: 780 114 213 342 1352 865 298 4428 Fragments arranged by size: 4428 1352 865 780 342 298 213 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3023 5048 7055 882 Size: 617 1524 2025 2007 2219 Fragments arranged by size: 2219 2025 2007 1524 617 BsaAI yAC'GTr Cuts at: 493 1204 2686 5119 5284 5689 6183 493 Size: 711 1482 2433 165 405 494 2702 Fragments arranged by size: 2702 2433 1482 711 494 405 165 BsaBI GATnn'nnATC Cuts at: 8386 8386 Size: 8392 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 2165 2405 2570 Size: 53 83 186 936 545 87 240 165 Cuts at: 2570 3531 3543 4358 5394 5625 5631 5670 6199 Size: 961 12 815 1036 231 6 39 529 Cuts at: 6199 6581 7216 275 Size: 382 635 1451 Fragments arranged by size: 1451 1036 961 936 815 635 545 529 382 240 231 186 165 87 83 53 39 12 6 BsaWI w'CCGG_w Cuts at: 6825 7223 7497 7644 8055 6825 Size: 398 274 147 411 7162 Fragments arranged by size: 7162 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2717 2747 2818 2848 3659 Size: 30 165 30 1314 30 71 30 811 Cuts at: 3659 3671 3689 3701 3833 3863 5045 5075 1178 Size: 12 18 12 132 30 1182 30 4495 Fragments arranged by size: 4495 1314 1182 811 165 132 71 30 30 30 30 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 3757 3760 3763 3766 3769 3772 3775 Size: 1087 1484 3 3 3 3 3 3 Cuts at: 3775 3878 3971 4018 4066 4228 4517 4574 4919 Size: 103 93 47 48 162 289 57 345 Cuts at: 4919 5012 1186 Size: 93 4566 Fragments arranged by size: 4566 1484 1087 345 289 162 103 93 93 57 48 47 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2182 3079 3097 3182 3653 4116 4586 4649 5049 Size: 897 18 85 471 463 470 63 400 Cuts at: 5049 7595 8091 2182 Size: 2546 496 2483 Fragments arranged by size: 2546 2483 897 496 471 470 463 400 85 63 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3068 5141 1163 Size: 573 1332 2073 4414 Fragments arranged by size: 4414 2073 1332 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3045 3311 3395 3422 3429 5842 Size: 275 186 1044 266 84 27 7 2413 Cuts at: 5842 6603 7631 1540 Size: 761 1028 2301 Fragments arranged by size: 2413 2301 1044 1028 761 275 266 186 84 27 7 BsiHKAI G_wGCw'C Cuts at: 2379 3476 5178 5283 5514 6453 6538 7609 2379 Size: 1097 1702 105 231 939 85 1071 3162 Fragments arranged by size: 3162 1702 1097 1071 939 231 105 85 BsmI GAATG_Cn' Cuts at: 5903 5996 5903 Size: 93 8299 BsmBI CGTCTCn'nnnn_ Cuts at: 2357 3032 2357 Size: 675 7717 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2131 2138 2379 2441 2617 2807 Size: 399 301 40 7 241 62 176 190 Cuts at: 2807 2823 2925 3172 3202 3275 3401 3476 3568 Size: 16 102 247 30 73 126 75 92 Cuts at: 3568 4047 4071 4117 4662 5178 5283 5514 5610 Size: 479 24 46 545 516 105 231 96 Cuts at: 5610 5711 6453 6538 7609 1391 Size: 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 545 516 479 399 301 247 241 231 190 176 126 105 102 101 96 92 85 75 73 62 46 40 30 24 16 7 BspEI T'CCGG_A Cuts at: 8055 8055 Size: 8392 BspHI T'CATG_A Cuts at: 6281 8011 6281 Size: 1730 6662 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2839 3184 4116 4710 4827 4946 5747 844 Size: 1995 345 932 594 117 119 801 3489 Fragments arranged by size: 3489 1995 932 801 594 345 119 117 BsrBI CCG'CTC Cuts at: 1394 1823 2382 2782 3307 3505 3516 4873 6279 Size: 429 559 400 525 198 11 1357 1406 Cuts at: 6279 1394 Size: 3507 Fragments arranged by size: 3507 1406 1357 559 525 429 400 198 11 BsrDI GCAATG_nn' Cuts at: 65 1248 6887 7061 65 Size: 1183 5639 174 1396 Fragments arranged by size: 5639 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2094 2510 3378 3421 3428 3918 5084 Size: 110 284 416 868 43 7 490 1166 Cuts at: 5084 5627 7036 7223 1700 Size: 543 1409 187 2869 Fragments arranged by size: 2869 1409 1166 868 543 490 416 284 187 110 43 7 BsrGI T'GTAC_A Cuts at: 96 4797 96 Size: 4701 3691 BssHII G'CGCG_C Cuts at: 1925 2142 1925 Size: 217 8175 BssSI C'ACGA_G Cuts at: 4700 4964 6452 7464 4700 Size: 264 1488 1012 5628 Fragments arranged by size: 5628 1488 1012 264 BstAPI GCAn_nnn'nTGC Cuts at: 794 4938 5119 794 Size: 4144 181 4067 Fragments arranged by size: 4144 4067 181 BstBI TT'CG_AA Cuts at: 2007 7196 2007 Size: 5189 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8392 BstXI CCAn_nnnn'nTGG Cuts at: 2530 2716 3349 2530 Size: 186 633 7573 Fragments arranged by size: 7573 633 186 BstYI r'GATC_y Cuts at: 1972 3876 4008 5199 5722 6089 6474 6491 7943 Size: 1904 132 1191 523 367 385 17 1452 Cuts at: 7943 8029 8041 8387 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1904 1452 1191 523 385 367 346 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3358 4808 3358 Size: 1450 6942 BtgI C'CryG_G Cuts at: 513 1065 2122 2410 2488 2809 2904 2928 3858 Size: 552 1057 288 78 321 95 24 930 Cuts at: 3858 4062 5493 513 Size: 204 1431 3412 Fragments arranged by size: 3412 1431 1057 930 552 321 288 204 95 78 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2883 487 Size: 683 56 1657 5996 Fragments arranged by size: 5996 1657 683 56 BtsI GCAGTG_nn' Cuts at: 2326 2821 3056 5908 6702 6722 2326 Size: 495 235 2852 794 20 3996 Fragments arranged by size: 3996 2852 794 495 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8392 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4660 4695 529 Size: 35 4096 35 4226 Fragments arranged by size: 4226 4096 35 35 DraI TTT'AAA Cuts at: 4422 5696 4422 Size: 1274 7118 DraIII CAC_nnn'GTG Cuts at: 4705 5647 4705 Size: 942 7450 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5378 7399 809 Size: 507 4062 2021 1802 Fragments arranged by size: 4062 2021 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2532 2931 3042 Size: 54 1425 50 275 18 702 399 111 Cuts at: 3042 3308 3339 3351 3380 3392 3426 4281 4321 Size: 266 31 12 29 12 34 855 40 Cuts at: 4321 4611 4681 4725 5040 5128 5565 5839 6728 Size: 290 70 44 315 88 437 274 889 Cuts at: 6728 8357 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 855 702 437 399 315 290 275 274 266 111 88 70 54 50 44 43 40 34 31 29 18 12 12 EagI C'GGCC_G Cuts at: 1537 1812 3042 3308 3392 3426 5839 1537 Size: 275 1230 266 84 34 2413 4090 Fragments arranged by size: 4090 2413 1230 275 266 84 34 EarI CTCTTCn'nnn_ Cuts at: 1353 3464 3939 3944 3995 4205 4904 4930 4978 Size: 2111 475 5 51 210 699 26 48 Cuts at: 4978 5519 6322 1353 Size: 541 803 3423 Fragments arranged by size: 3423 2111 803 699 541 475 210 51 48 26 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2172 2374 3821 4186 4463 6979 Size: 1064 138 583 202 1447 365 277 2516 Cuts at: 6979 7353 7499 387 Size: 374 146 1280 Fragments arranged by size: 2516 1447 1280 1064 583 374 365 277 202 146 138 EcoICRI GAG'CTC Cuts at: 5176 5176 Size: 8392 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2354 2381 2683 2738 3023 3053 Size: 393 668 54 27 302 55 285 30 Cuts at: 3053 3437 3575 3673 3778 3848 4959 5264 5474 Size: 384 138 98 105 70 1111 305 210 Cuts at: 5474 5495 5599 6455 7052 7839 8107 1239 Size: 21 104 856 597 787 268 1524 Fragments arranged by size: 1524 1111 856 787 668 597 393 384 305 302 285 268 210 138 105 104 98 70 55 54 30 27 21 EcoNI CCTnn'n_nnAGG Cuts at: 2255 3830 4314 2255 Size: 1575 484 6333 Fragments arranged by size: 6333 1575 484 EcoO109I rG'GnC_Cy Cuts at: 2087 2088 2127 2134 2135 2392 2998 3064 3168 Size: 1 39 7 1 257 606 66 104 Cuts at: 3168 3169 3198 3271 3481 3565 4058 4153 4272 Size: 1 29 73 210 84 493 95 119 Cuts at: 4272 4639 4777 4935 2087 Size: 367 138 158 5544 Fragments arranged by size: 5544 606 493 367 257 210 158 138 119 104 95 84 73 66 39 29 7 1 1 1 EcoRI G'AATT_C Cuts at: 5701 6079 5701 Size: 378 8014 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4756 4756 Size: 8392 FspI TGC'GCA Cuts at: 2318 6898 2318 Size: 4580 3812 HaeII r_GCGC'y Cuts at: 1678 2081 2168 2347 2408 3534 4483 4656 5143 Size: 403 87 179 61 1126 949 173 487 Cuts at: 5143 5427 5634 7539 1678 Size: 284 207 1905 2531 Fragments arranged by size: 2531 1905 1126 949 487 403 284 207 179 173 87 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2717 2749 3659 3665 3671 Size: 32 135 32 1145 32 910 6 6 Cuts at: 3671 3691 3697 3703 3833 3865 4042 4074 5571 Size: 20 6 6 130 32 177 32 1497 Cuts at: 5571 5603 7033 7065 7107 7139 1373 Size: 32 1430 32 42 32 2626 Fragments arranged by size: 2626 1497 1430 1145 910 177 135 130 42 32 32 32 32 32 32 32 32 20 6 6 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2296 5269 5736 5982 669 Size: 988 281 358 2973 467 246 3079 Fragments arranged by size: 3079 2973 988 467 358 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8392 HpaI GTT'AAC Cuts at: 5982 5982 Size: 8392 KasI G'GCGC_C Cuts at: 2077 2164 2404 3530 5630 2077 Size: 87 240 1126 2100 4839 Fragments arranged by size: 4839 2100 1126 240 87 KpnI G_GTAC'C Cuts at: 5717 5717 Size: 8392 MfeI C'AATT_G Cuts at: 5991 5991 Size: 8392 MluI A'CGCG_T Cuts at: 8268 8268 Size: 8392 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 2760 3702 3876 4326 4551 7506 7690 Size: 960 495 942 174 450 225 2955 184 Cuts at: 7690 1305 Size: 2007 Fragments arranged by size: 2955 2007 960 942 495 450 225 184 174 MscI TGG'CCA Cuts at: 10 64 1489 1832 3353 4323 10 Size: 54 1425 343 1521 970 4079 Fragments arranged by size: 4079 1521 1425 970 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3347 5276 5300 5495 5507 6350 6709 Size: 648 2181 1929 24 195 12 843 359 Cuts at: 6709 6868 518 Size: 159 2042 Fragments arranged by size: 2181 2042 1929 843 648 359 195 159 24 12 MspA1I CmG'CkG Cuts at: 1606 2124 2341 2412 2811 3186 3457 4064 4287 Size: 518 217 71 399 375 271 607 223 Cuts at: 4287 4330 4901 4947 4983 5808 6485 7633 7878 Size: 43 571 46 36 825 677 1148 245 Cuts at: 7878 1606 Size: 2120 Fragments arranged by size: 2120 1148 825 677 607 571 518 399 375 271 245 223 217 71 46 43 36 NaeI GCC'GGC Cuts at: 2096 2512 3430 3920 5086 5629 2096 Size: 416 918 490 1166 543 4859 Fragments arranged by size: 4859 1166 918 543 490 416 NarI GG'CG_CC Cuts at: 2078 2165 2405 3531 5631 2078 Size: 87 240 1126 2100 4839 Fragments arranged by size: 4839 2100 1126 240 87 NcoI C'CATG_G Cuts at: 513 1065 3858 513 Size: 552 2793 5047 Fragments arranged by size: 5047 2793 552 NdeI CA'TA_TG Cuts at: 387 5236 387 Size: 4849 3543 NgoMIV G'CCGG_C Cuts at: 2094 2510 3428 3918 5084 5627 2094 Size: 416 918 490 1166 543 4859 Fragments arranged by size: 4859 1166 918 543 490 416 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8392 NotI GC'GGCC_GC Cuts at: 5839 5839 Size: 8392 NspI r_CATG'y Cuts at: 1132 1229 5068 5419 5641 5750 7295 1132 Size: 97 3839 351 222 109 1545 2229 Fragments arranged by size: 3839 2229 1545 351 222 109 97 PasI CC'CwG_GG Cuts at: 1348 2245 3202 1348 Size: 897 957 6538 Fragments arranged by size: 6538 957 897 PciI A'CATG_T Cuts at: 5064 7291 5064 Size: 2227 6165 PflMI CCAn_nnn'nTGG Cuts at: 1108 4039 4705 5527 1108 Size: 2931 666 822 3973 Fragments arranged by size: 3973 2931 822 666 PfoI T'CCnGG_A Cuts at: 3702 4989 5589 3702 Size: 1287 600 6505 Fragments arranged by size: 6505 1287 600 PmeI GTTT'AAAC Cuts at: 5696 5696 Size: 8392 PmlI CAC'GTG Cuts at: 5119 5284 5119 Size: 165 8227 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6461 6493 7974 8006 6461 Size: 32 1481 32 6847 Fragments arranged by size: 6847 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3064 4058 4272 4777 4935 3064 Size: 994 214 505 158 6521 Fragments arranged by size: 6521 994 505 214 158 PshAI GACnn'nnGTC Cuts at: 1613 2583 5255 1613 Size: 970 2672 4750 Fragments arranged by size: 4750 2672 970 PsiI TTA'TAA Cuts at: 5962 5962 Size: 8392 PspOMI G'GGCC_C Cuts at: 2087 2127 2134 2437 3168 3198 3271 3564 4113 Size: 40 7 303 731 30 73 293 549 Cuts at: 4113 2087 Size: 6366 Fragments arranged by size: 6366 731 549 303 293 73 40 30 7 PstI C_TGCA'G Cuts at: 830 4152 4252 4934 5744 830 Size: 3322 100 682 810 3478 Fragments arranged by size: 3478 3322 810 682 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8392 PvuII CAG'CTG Cuts at: 3186 3457 4330 4901 3186 Size: 271 873 571 6677 Fragments arranged by size: 6677 873 571 271 SacI G_AGCT'C Cuts at: 5178 5178 Size: 8392 SacII CC_GC'GG Cuts at: 2125 2413 2812 4065 2125 Size: 288 399 1253 6452 Fragments arranged by size: 6452 1253 399 288 SalI G'TCGA_C Cuts at: 1936 5267 5734 1936 Size: 3331 467 4594 Fragments arranged by size: 4594 3331 467 SanDI GG'GwC_CC Cuts at: 4058 4058 Size: 8392 SapI GCTCTTCn'nnn_ Cuts at: 3464 5519 3464 Size: 2055 6337 SbfI CC_TGCA'GG Cuts at: 4934 5744 4934 Size: 810 7582 ScaI AGT'ACT Cuts at: 2561 6640 8352 2561 Size: 4079 1712 2601 Fragments arranged by size: 4079 2601 1712 SexAI A'CCwGG_T Cuts at: 5336 5643 5336 Size: 307 8085 SfcI C'TryA_G Cuts at: 826 1045 2473 4148 4248 4930 5740 6875 7556 Size: 219 1428 1675 100 682 810 1135 681 Cuts at: 7556 7747 826 Size: 191 1471 Fragments arranged by size: 1675 1471 1428 1135 810 682 681 219 191 100 SfiI GGCCn_nnn'nGGCC Cuts at: 2309 3379 2309 Size: 1070 7322 SfoI GGC'GCC Cuts at: 2079 2166 2406 3532 5632 2079 Size: 87 240 1126 2100 4839 Fragments arranged by size: 4839 2100 1126 240 87 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8392 SgrAI Cr'CCGG_yG Cuts at: 1700 5627 1700 Size: 3927 4465 SmaI CCC'GGG Cuts at: 1872 2083 2132 2774 2801 3994 4769 4879 5196 Size: 211 49 642 27 1193 775 110 317 Cuts at: 5196 5719 1872 Size: 523 4545 Fragments arranged by size: 4545 1193 775 642 523 317 211 110 49 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 3731 4511 4625 4838 5144 6496 Size: 197 926 1788 780 114 213 306 1352 Cuts at: 6496 7397 7659 820 Size: 901 262 1553 Fragments arranged by size: 1788 1553 1352 926 901 780 306 262 213 197 114 SnaBI TAC'GTA Cuts at: 493 5689 6183 493 Size: 5196 494 2702 Fragments arranged by size: 5196 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8392 SphI G_CATG'C Cuts at: 1132 5641 5750 1132 Size: 4509 109 3774 Fragments arranged by size: 4509 3774 109 SspI AAT'ATT Cuts at: 5 52 6316 5 Size: 47 6264 2081 Fragments arranged by size: 6264 2081 47 StuI AGG'CCT Cuts at: 1517 3373 3737 1517 Size: 1856 364 6172 Fragments arranged by size: 6172 1856 364 StyI C'CwwG_G Cuts at: 513 1065 2631 3033 3135 3219 3477 3858 3939 Size: 552 1566 402 102 84 258 381 81 Cuts at: 3939 4917 5831 513 Size: 978 914 3074 Fragments arranged by size: 3074 1566 978 914 552 402 381 258 102 84 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2722 4841 4881 5204 6419 6436 6589 Size: 1255 1142 2119 40 323 1215 17 153 Cuts at: 6589 6774 7219 325 Size: 185 445 1498 Fragments arranged by size: 2119 1498 1255 1215 1142 445 323 185 153 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2559 4797 5061 Size: 274 80 33 51 1443 582 2238 264 Cuts at: 5061 6638 8350 96 Size: 1577 1712 138 Fragments arranged by size: 2238 1712 1577 1443 582 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2818 2850 4391 4423 4985 5017 8352 8384 2818 Size: 32 1541 32 562 32 3335 32 2826 Fragments arranged by size: 3335 2826 1541 562 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8392 XbaI T'CTAG_A Cuts at: 5728 5728 Size: 8392 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2431 2998 4240 5239 2431 Size: 567 1242 999 5584 Fragments arranged by size: 5584 1242 999 567 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8392 XmaI C'CCGG_G Cuts at: 1870 2081 2130 2772 2799 3992 4767 4877 5194 Size: 211 49 642 27 1193 775 110 317 Cuts at: 5194 5717 1870 Size: 523 4545 Fragments arranged by size: 4545 1193 775 642 523 317 211 110 49 27 XmnI GAAnn'nnTTC Cuts at: 6521 6521 Size: 8392 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5395 6200 276 Size: 53 83 186 936 3861 805 2468 Fragments arranged by size: 3861 2468 936 805 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SrfI SwaI