Restriction map for
pFN21ASDA1130
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DrdI DsaI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HincII HindII
HindIII HpaI KasI KspI MfeI
MluI MluNI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1241 GGTACC G^GTACC
AccIII 501 TCCGGA T^CCGGA
AcsI 960 GAATTT [GA]^AATT[CT]
AcyI 864 GACGCC G[GA]^CG[CT]C
AfaI 1242 GTAC GT^AC
AlwNI 755 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 960 GAATTT [GA]^AATT[CT]
Asp700I 1173 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1241 GGTACC G^GTACC
BfaI 1523 CTAG C^TAG
BlpI 294 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 294 GCTGAGC GC^T[ACGT]AGC
BsaBI 939 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 864 GACGCC G[GA]^CG[CT]C
BseAI 501 TCCGGA T^CCGGA
BseRI 354 CTCCTC GAGGAG(10/8)
BsiEI 108 CGGCCG CG[GA][CT]^CG
BsmAI 1544 GTCTC GTCTC(1/5)
BspEI 501 TCCGGA T^CCGGA
BsrBI 169 CCGCTC CCGCTC(-3/-3)
BsrDI 551 GCAATG GCAATG(2/0)
BsrFI 305 ACCGGC [GA]^CCGG[CT]
BssHII 116 GCGCGC G^CGCGC
BstAPI 754 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 294 GCTGAGC GC^T[ACGT]AGC
Cfr10I 305 ACCGGC [GA]^CCGG[CT]
DraII 248 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 651 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 108 CGGCCG C^GGCCG
EarI 429 GAAGAG CTCTTC(1/4)
EclXI 108 CGGCCG C^GGCCG
Eco52I 108 CGGCCG C^GGCCG
EcoO109I 248 AGGACCC [GA]G^G[ACGT]CC[CT]
Hin1I 864 GACGCC G[GA]^CG[CT]C
KpnI 1241 GGTACC GGTAC^C
Ksp632I 429 GAAGAG CTCTTC(1/4)
MaeI 1523 CTAG C^TAG
MamI 939 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 501 TCCGGA T^CCGGA
PpuMI 248 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 864 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 1242 GTAC GT^AC
SmlI 820 CTTGAG C^T[CT][GA]AG
XcmI 1338 CCAGAACACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1173 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 512 GTCTAC GT^[AC][GT]AC
1255 GTCTAC
AocI 1066 CCTGAGG CC^T[ACGT]AGG
1590 CCTCAGG
AspHI 967 GAGCTC G[AT]GC[AT]^C
1264 GAGCTC
AvaI 581 CCCGAG C^[CT]CG[GA]G
1576 CCCGAG
AviII 419 TGCGCA TGC^GCA
548 TGCGCA
BanI 1049 GGCACC G^G[CT][GA]CC
1241 GGTACC
BbsI 505 GAAGAC GAAGAC(2/6)
1036 GTCTTC
BglI 1022 GCCGGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1575 GCCCGAGGGGC
BpuAI 505 GAAGAC GAAGAC(2/6)
1036 GTCTTC
BsaWI 501 TCCGGA [AT]^CCGG[AT]
575 TCCGGT
BsiHKAI 967 GAGCTC G[AT]GC[AT]^C
1264 GAGCTC
BsoBI 581 CCCGAG C^[CT]CG[GA]G
1576 CCCGAG
BspMI 362 ACCTGC ACCTGC(4/8)
1536 GCAGGT
BsrI 785 ACTGG ACTGG(1/-1)
1498 CCAGT
Bsu36I 1066 CCTGAGG CC^T[ACGT]AGG
1590 CCTCAGG
Eco81I 1066 CCTGAGG CC^T[ACGT]AGG
1590 CCTCAGG
EcoNI 1085 CCTACATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1522 CCTAGAGAAGG
EcoT14I 533 CCAAGG C^C[AT][AT]GG
1605 CCTTGG
FspI 419 TGCGCA TGC^GCA
548 TGCGCA
HgaI 864 GACGC GACGC(5/10)
377 GCGTC
MaeII 599 ACGT A^CGT
1253 ACGT
PleI 708 GAGTC GAGTC(4/5)
1542 GAGTC
SacI 967 GAGCTC GAGCT^C
1264 GAGCTC
StuI 212 AGGCCT AGG^CCT
1175 AGGCCT
StyI 533 CCAAGG C^C[AT][AT]GG
1605 CCTTGG
TfiI 884 GAATC G^A[AT]TC
1327 GAATC
Tsp509I 739 AATT ^AATT
961 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BamHI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I XhoII