(Circular) (Six-base) MAPSORT of: pFN21ASDA1130.seq Check: 7178 from: 1 to: 6355 pFN21ASDA1130 6355 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3550 3550 Size: 6355 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2536 3279 3698 1937 Size: 599 743 419 4594 Fragments arranged by size: 4594 743 599 419 Acc65I G'GTAC_C Cuts at: 3264 3676 3264 Size: 412 5943 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2279 2497 2983 3046 4418 5802 6070 1632 Size: 647 218 486 63 1372 1384 268 1917 Fragments arranged by size: 1917 1384 1372 647 486 268 218 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6158 AflIII A'CryG_T Cuts at: 1203 5254 6231 1203 Size: 4051 977 1327 Fragments arranged by size: 4051 1327 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 5084 5084 Size: 6355 AleI CACnn'nnGTG Cuts at: 1166 2723 1166 Size: 1557 4798 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6355 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3236 3268 3236 Size: 32 6323 AlwNI CAG_nnn'CTG Cuts at: 2783 4098 5670 2783 Size: 1315 1572 3468 Fragments arranged by size: 3468 1572 1315 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 2983 3664 3890 4042 6151 1493 Size: 1490 681 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 681 226 152 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 2604 3599 3680 1870 Size: 73 661 995 81 4545 Fragments arranged by size: 4545 995 661 81 73 BamHI G'GATC_C Cuts at: 2070 2550 3515 3685 4052 2070 Size: 480 965 170 367 4373 Fragments arranged by size: 4373 965 480 367 170 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3072 3264 3676 5131 618 Size: 325 217 627 1285 192 412 1455 1842 Fragments arranged by size: 1842 1455 1285 627 412 325 217 192 BanII G_rGCy'C Cuts at: 1391 2113 2324 2994 3291 3643 3674 1391 Size: 722 211 670 297 352 31 4072 Fragments arranged by size: 4072 722 670 352 297 211 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2535 3052 928 Size: 971 636 517 4231 Fragments arranged by size: 4231 971 636 517 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4546 4580 1903 Size: 34 2609 34 3678 Fragments arranged by size: 3678 2609 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4247 5457 4247 Size: 1210 5145 BclI T'GATC_A Cuts at: 1548 1548 Size: 6355 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3051 3604 4966 Size: 107 122 71 631 605 1379 553 1362 Cuts at: 4966 136 Size: 1525 Fragments arranged by size: 1525 1379 1362 631 605 553 122 107 71 BglII A'GATC_T Cuts at: 6350 6350 Size: 6355 BlpI GC'TnA_GC Cuts at: 2318 3772 2318 Size: 1454 4901 Bme1580I G_kGCm'C Cuts at: 1790 2081 4416 5572 1790 Size: 291 2335 1156 2573 Fragments arranged by size: 2573 2335 1156 291 BmgBI CAC'GTC Cuts at: 1318 2623 1318 Size: 1305 5050 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3515 4086 5044 Size: 416 53 83 399 24 2098 571 958 Cuts at: 5044 442 Size: 1753 Fragments arranged by size: 2098 1753 958 571 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2286 2324 2830 2922 3047 3290 5015 1239 Size: 1047 38 506 92 125 243 1725 2579 Fragments arranged by size: 2579 1725 1047 506 243 125 92 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2864 4480 5345 5643 2864 Size: 1616 865 298 3576 Fragments arranged by size: 3576 1616 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5018 882 Size: 617 3519 2219 Fragments arranged by size: 3519 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3652 4146 493 Size: 711 2448 494 2702 Fragments arranged by size: 2702 2448 711 494 BsaBI GATnn'nnATC Cuts at: 2966 6349 2966 Size: 3383 2972 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2888 4162 4544 5179 Size: 53 83 186 936 1355 1274 382 635 Cuts at: 5179 275 Size: 1451 Fragments arranged by size: 1451 1355 1274 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2524 2598 4788 5186 5460 5607 6018 2524 Size: 74 2190 398 274 147 411 2861 Fragments arranged by size: 2861 2190 411 398 274 147 74 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2194 2224 1178 Size: 30 165 30 791 30 5309 Fragments arranged by size: 5309 791 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2368 1186 Size: 1182 5173 BseYI C'CCAG_C Cuts at: 3591 5558 6054 3591 Size: 1967 496 3892 Fragments arranged by size: 3892 1967 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5782 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2134 3805 4566 5594 1540 Size: 275 186 133 1671 761 1028 2301 Fragments arranged by size: 2301 1671 1028 761 275 186 133 BsiHKAI G_wGCw'C Cuts at: 2994 3291 4416 4501 5572 2994 Size: 297 1125 85 1071 3777 Fragments arranged by size: 3777 1125 1071 297 85 BsmI GAATG_Cn' Cuts at: 3866 3959 3866 Size: 93 6262 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2113 2324 2994 3291 3643 3674 Size: 399 291 32 211 670 297 352 31 Cuts at: 3674 4416 4501 5572 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 670 399 352 297 291 211 85 32 31 BspEI T'CCGG_A Cuts at: 2524 6018 2524 Size: 3494 2861 BspHI T'CATG_A Cuts at: 4244 5974 4244 Size: 1730 4625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2394 3550 3710 844 Size: 1550 1156 160 3489 Fragments arranged by size: 3489 1550 1156 160 BsrBI CCG'CTC Cuts at: 1394 1823 2194 4242 1394 Size: 429 371 2048 3507 Fragments arranged by size: 3507 2048 429 371 BsrDI GCAATG_nn' Cuts at: 65 1248 2581 4850 5024 65 Size: 1183 1333 2269 174 1396 Fragments arranged by size: 2269 1396 1333 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2328 4999 5186 1700 Size: 110 518 2671 187 2869 Fragments arranged by size: 2869 2671 518 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6355 BssHII G'CGCG_C Cuts at: 1925 2139 1925 Size: 214 6141 BssSI C'ACGA_G Cuts at: 4415 5427 4415 Size: 1012 5343 BstAPI GCAn_nnn'nTGC Cuts at: 794 2783 794 Size: 1989 4366 BstBI TT'CG_AA Cuts at: 2007 5159 2007 Size: 3152 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6355 BstYI r'GATC_y Cuts at: 1972 2031 2070 2521 2550 2835 3396 3515 3685 Size: 59 39 451 29 285 561 119 170 Cuts at: 3685 4052 4437 4454 5906 5992 6004 6350 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 561 451 385 367 346 285 170 119 86 59 39 29 17 12 Bsu36I CC'TnA_GG Cuts at: 3090 3614 3090 Size: 524 5831 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5803 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2032 487 Size: 683 56 806 4810 Fragments arranged by size: 4810 806 683 56 BtsI GCAGTG_nn' Cuts at: 2659 3129 3214 3871 4665 4685 2659 Size: 470 85 657 794 20 4329 Fragments arranged by size: 4329 794 657 470 85 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6320 DraI TTT'AAA Cuts at: 3659 3659 Size: 6355 DraIII CAC_nnn'GTG Cuts at: 2679 2679 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5362 809 Size: 507 4046 1802 Fragments arranged by size: 4046 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2131 2247 3186 Size: 54 1425 50 275 18 301 116 939 Cuts at: 3186 3802 4691 6320 8 Size: 616 889 1629 43 Fragments arranged by size: 1629 1425 939 889 616 301 275 116 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2131 3802 1537 Size: 275 319 1671 4090 Fragments arranged by size: 4090 1671 319 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2447 4285 1353 Size: 1094 1838 3423 Fragments arranged by size: 3423 1838 1094 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2633 4942 5316 5462 387 Size: 1064 138 1044 2309 374 146 1280 Fragments arranged by size: 2309 1280 1064 1044 374 146 138 EcoICRI GAG'CTC Cuts at: 2992 3289 2992 Size: 297 6058 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2286 2324 2497 2830 2922 2983 Size: 393 647 7 38 173 333 92 61 Cuts at: 2983 3046 3047 3290 4418 5015 5802 6070 1239 Size: 63 1 243 1128 597 787 268 1524 Fragments arranged by size: 1524 1128 787 647 597 393 333 268 243 173 92 63 61 38 7 1 EcoNI CCTnn'n_nnAGG Cuts at: 3112 3549 3112 Size: 437 5918 EcoO109I rG'GnC_Cy Cuts at: 2272 2272 Size: 6355 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 FspI TGC'GCA Cuts at: 2444 2573 4861 2444 Size: 129 2288 3938 Fragments arranged by size: 3938 2288 129 HaeII r_GCGC'y Cuts at: 1678 5502 1678 Size: 3824 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2194 2226 2501 2533 3507 Size: 32 135 32 622 32 275 32 974 Cuts at: 3507 3539 4996 5028 5070 5102 1373 Size: 32 1457 32 42 32 2626 Fragments arranged by size: 2626 1457 974 622 275 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3699 3945 669 Size: 988 281 1761 246 3079 Fragments arranged by size: 3079 1761 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6355 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KpnI G_GTAC'C Cuts at: 3268 3680 3268 Size: 412 5943 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2142 5469 5653 1305 Size: 837 3327 184 2007 Fragments arranged by size: 3327 2007 837 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4533 Fragments arranged by size: 4533 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2501 2723 3500 4313 4672 4831 518 Size: 648 1335 222 777 813 359 159 2042 Fragments arranged by size: 2042 1335 813 777 648 359 222 159 MspA1I CmG'CkG Cuts at: 1606 2257 2302 2358 2701 3261 3371 3771 4448 Size: 651 45 56 343 560 110 400 677 Cuts at: 4448 5596 5841 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 651 560 400 343 245 110 56 45 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5803 NdeI CA'TA_TG Cuts at: 387 387 Size: 6355 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 3713 5258 1132 Size: 97 2484 1545 2229 Fragments arranged by size: 2484 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6355 PciI A'CATG_T Cuts at: 5254 5254 Size: 6355 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6355 PfoI T'CCnGG_A Cuts at: 2337 2487 2337 Size: 150 6205 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4424 4456 5937 5969 4424 Size: 32 1481 32 4810 Fragments arranged by size: 4810 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2272 2272 Size: 6355 PshAI GACnn'nnGTC Cuts at: 1613 2891 1613 Size: 1278 5077 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PstI C_TGCA'G Cuts at: 830 2632 2700 3044 3136 3404 3534 3707 830 Size: 1802 68 344 92 268 130 173 3478 Fragments arranged by size: 3478 1802 344 268 173 130 92 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 PvuII CAG'CTG Cuts at: 2257 2302 2358 3371 2257 Size: 45 56 1013 5241 Fragments arranged by size: 5241 1013 56 45 SacI G_AGCT'C Cuts at: 2994 3291 2994 Size: 297 6058 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SbfI CC_TGCA'GG Cuts at: 3707 3707 Size: 6355 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SfcI C'TryA_G Cuts at: 826 1045 2278 2628 2696 3040 3132 3400 3530 Size: 219 1233 350 68 344 92 268 130 Cuts at: 3530 3703 4838 5519 5710 826 Size: 173 1135 681 191 1471 Fragments arranged by size: 1471 1233 1135 681 350 344 268 219 191 173 130 92 68 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3682 1872 Size: 1810 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2843 4459 5360 5622 820 Size: 197 926 900 1616 901 262 1553 Fragments arranged by size: 1616 1553 926 901 900 262 197 SnaBI TAC'GTA Cuts at: 493 3652 4146 493 Size: 3159 494 2702 Fragments arranged by size: 3159 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 3713 1132 Size: 2581 3774 SspI AAT'ATT Cuts at: 5 52 4279 5 Size: 47 4227 2081 Fragments arranged by size: 4227 2081 47 StuI AGG'CCT Cuts at: 1517 2237 3200 1517 Size: 720 963 4672 Fragments arranged by size: 4672 963 720 StyI C'CwwG_G Cuts at: 513 1065 2556 3628 3794 513 Size: 552 1491 1072 166 3074 Fragments arranged by size: 3074 1491 1072 552 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4382 4399 4552 4737 5182 325 Size: 1255 2802 17 153 185 445 1498 Fragments arranged by size: 2802 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4601 6313 96 Size: 274 80 33 51 1443 2624 1712 138 Fragments arranged by size: 2624 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2105 2137 2898 2930 3001 3033 6315 6347 2105 Size: 32 761 32 71 32 3282 32 2113 Fragments arranged by size: 3282 2113 761 71 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6355 XbaI T'CTAG_A Cuts at: 3691 3691 Size: 6355 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3368 3368 Size: 6355 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6355 XmaI C'CCGG_G Cuts at: 1870 3680 1870 Size: 1810 4545 XmnI GAAnn'nnTTC Cuts at: 3200 4484 3200 Size: 1284 5071 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI