Restriction map for
pFN21ASDA1130
(Linear) (Six-base) MAPSORT of: KIAA1130.seq Check: 3492 from: 1 to: 1626
KIAA1130.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1527 1626
Size: 1527 99
AccI GT'mk_AC
Cuts at: 0 513 1256 1626
Size: 513 743 370
Fragments arranged by size:
743 513 370
Acc65I G'GTAC_C
Cuts at: 0 1241 1626
Size: 1241 385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 474 960 1023 1626
Size: 256 218 486 63 603
Fragments arranged by size:
603 486 256 218 63
AleI CACnn'nnGTG
Cuts at: 0 700 1626
Size: 700 926
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1213 1245 1626
Size: 1213 32 381
Fragments arranged by size:
1213 381 32
AlwNI CAG_nnn'CTG
Cuts at: 0 760 1626
Size: 760 866
ApoI r'AATT_y
Cuts at: 0 960 1626
Size: 960 666
AvaI C'yCGr_G
Cuts at: 0 581 1576 1626
Size: 581 995 50
Fragments arranged by size:
995 581 50
BamHI G'GATC_C
Cuts at: 0 47 527 1492 1626
Size: 47 480 965 134
Fragments arranged by size:
965 480 134 47
BanI G'GyrC_C
Cuts at: 0 1049 1241 1626
Size: 1049 192 385
Fragments arranged by size:
1049 385 192
BanII G_rGCy'C
Cuts at: 0 90 301 971 1268 1620 1626
Size: 90 211 670 297 352 6
Fragments arranged by size:
670 352 297 211 90 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 512 1029 1626
Size: 512 517 597
Fragments arranged by size:
597 517 512
BglI GCCn_nnn'nGGC
Cuts at: 0 1028 1581 1626
Size: 1028 553 45
Fragments arranged by size:
1028 553 45
BlpI GC'TnA_GC
Cuts at: 0 295 1626
Size: 295 1331
Bme1580I G_kGCm'C
Cuts at: 0 58 1626
Size: 58 1568
BmgBI CAC'GTC
Cuts at: 0 600 1626
Size: 600 1026
BmrI ACTGGGnnnn_n'
Cuts at: 0 1492 1626
Size: 1492 134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 301 807 899 1024 1267 1626
Size: 263 38 506 92 125 243 359
Fragments arranged by size:
506 359 263 243 125 92 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1626
Size: 841 785
BsaBI GATnn'nnATC
Cuts at: 0 943 1626
Size: 943 683
BsaHI Gr'CG_yC
Cuts at: 0 865 1626
Size: 865 761
BsaWI w'CCGG_w
Cuts at: 0 501 575 1626
Size: 501 74 1051
Fragments arranged by size:
1051 501 74
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 171 201 1626
Size: 171 30 1425
Fragments arranged by size:
1425 171 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 345 1626
Size: 345 1281
BseYI C'CCAG_C
Cuts at: 0 1568 1626
Size: 1568 58
BsiEI CG_ry'CG
Cuts at: 0 111 1626
Size: 111 1515
BsiHKAI G_wGCw'C
Cuts at: 0 971 1268 1626
Size: 971 297 358
Fragments arranged by size:
971 358 297
Bsp1286I G_dGCh'C
Cuts at: 0 58 90 301 971 1268 1620 1626
Size: 58 32 211 670 297 352 6
Fragments arranged by size:
670 352 297 211 58 32 6
BspEI T'CCGG_A
Cuts at: 0 501 1626
Size: 501 1125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 371 1527 1626
Size: 371 1156 99
Fragments arranged by size:
1156 371 99
BsrBI CCG'CTC
Cuts at: 0 171 1626
Size: 171 1455
BsrDI GCAATG_nn'
Cuts at: 0 558 1626
Size: 558 1068
BsrFI r'CCGG_y
Cuts at: 0 305 1626
Size: 305 1321
BssHII G'CGCG_C
Cuts at: 0 116 1626
Size: 116 1510
BstAPI GCAn_nnn'nTGC
Cuts at: 0 760 1626
Size: 760 866
BstYI r'GATC_y
Cuts at: 0 8 47 498 527 812 1373 1492 1626
Size: 8 39 451 29 285 561 119 134
Fragments arranged by size:
561 451 285 134 119 39 29 8
Bsu36I CC'TnA_GG
Cuts at: 0 1067 1591 1626
Size: 1067 524 35
Fragments arranged by size:
1067 524 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 9 1626
Size: 9 1617
BtsI GCAGTG_nn'
Cuts at: 0 636 1106 1191 1626
Size: 636 470 85 435
Fragments arranged by size:
636 470 435 85
DraIII CAC_nnn'GTG
Cuts at: 0 656 1626
Size: 656 970
EaeI y'GGCC_r
Cuts at: 0 108 224 1163 1626
Size: 108 116 939 463
Fragments arranged by size:
939 463 116 108
EagI C'GGCC_G
Cuts at: 0 108 1626
Size: 108 1518
EarI CTCTTCn'nnn_
Cuts at: 0 424 1626
Size: 424 1202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 610 1626
Size: 610 1016
EcoICRI GAG'CTC
Cuts at: 0 969 1266 1626
Size: 969 297 360
Fragments arranged by size:
969 360 297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 263 301 474 807 899 960 1023
Size: 256 7 38 173 333 92 61 63
Cuts at: 1023 1024 1267 1626
Size: 1 243 359
Fragments arranged by size:
359 333 256 243 173 92 63 61
38 7 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1089 1526 1626
Size: 1089 437 100
Fragments arranged by size:
1089 437 100
EcoO109I rG'GnC_Cy
Cuts at: 0 249 1626
Size: 249 1377
FspI TGC'GCA
Cuts at: 0 421 550 1626
Size: 421 129 1076
Fragments arranged by size:
1076 421 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 171 203 478 510 1484 1516 1626
Size: 171 32 275 32 974 32 110
Fragments arranged by size:
974 275 171 110 32 32 32
KpnI G_GTAC'C
Cuts at: 0 1245 1626
Size: 1245 381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1626
Size: 119 1507
MslI CAynn'nnrTG
Cuts at: 0 478 700 1477 1626
Size: 478 222 777 149
Fragments arranged by size:
777 478 222 149
MspA1I CmG'CkG
Cuts at: 0 234 279 335 678 1238 1348 1626
Size: 234 45 56 343 560 110 278
Fragments arranged by size:
560 343 278 234 110 56 45
PfoI T'CCnGG_A
Cuts at: 0 314 464 1626
Size: 314 150 1162
Fragments arranged by size:
1162 314 150
PpuMI rG'GwC_Cy
Cuts at: 0 249 1626
Size: 249 1377
PshAI GACnn'nnGTC
Cuts at: 0 868 1626
Size: 868 758
PstI C_TGCA'G
Cuts at: 0 609 677 1021 1113 1381 1511 1626
Size: 609 68 344 92 268 130 115
Fragments arranged by size:
609 344 268 130 115 92 68
PvuII CAG'CTG
Cuts at: 0 234 279 335 1348 1626
Size: 234 45 56 1013 278
Fragments arranged by size:
1013 278 234 56 45
SacI G_AGCT'C
Cuts at: 0 971 1268 1626
Size: 971 297 358
Fragments arranged by size:
971 358 297
SfcI C'TryA_G
Cuts at: 0 255 605 673 1017 1109 1377 1507 1626
Size: 255 350 68 344 92 268 130 119
Fragments arranged by size:
350 344 268 255 130 119 92 68
SmlI C'TyrA_G
Cuts at: 0 820 1626
Size: 820 806
StuI AGG'CCT
Cuts at: 0 214 1177 1626
Size: 214 963 449
Fragments arranged by size:
963 449 214
StyI C'CwwG_G
Cuts at: 0 533 1605 1626
Size: 533 1072 21
Fragments arranged by size:
1072 533 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 82 114 875 907 978 1010 1626
Size: 82 32 761 32 71 32 616
Fragments arranged by size:
761 616 82 71 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1345 1626
Size: 1345 281
XmnI GAAnn'nnTTC
Cuts at: 0 1177 1626
Size: 1177 449
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AloI AlwNI ApoI
AvaI BamHI BanI BanII BbsI BglI BlpI Bme1580I
BmgBI BmrI BpmI BpuEI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssHII BstAPI BstYI Bsu36I BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI Hin4I KpnI MmeI MslI MspA1I PfoI
PpuMI PshAI PstI PvuII SacI SfcI SmlI StuI
StyI TstI XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglII BmtI BplI
Bpu10I BsaI BsaAI BsgI BsiWI BsmI BsmBI BspHI
BsrGI BssSI BstBI BstEII BstXI BstZ17I BtgI ClaI
CspCI DraI DrdI EcoRI EcoRV FalI FseI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI ZraI