Restriction map for pFN21ASDA1099
##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AclI        AflII       AocI        AscI        AseI        
AsnI        AspI        AvrII       BbeI        BbrPI       
BbvCI       BciVI       BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsiWI       Bsp1407I    
BspHI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       CpoI        DraI        Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
HincII      HindII      HindIII     HpaI        KasI        
KspI        MamI        MfeI        MluI        MunI        
NaeI        NarI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        2221  GACGTC               GACGT^C
Acc65I        269  GGTACC               G^GTACC
AccI         1942  GTAGAC               GT^[AC][GT]AC
AcsI         1497  AAATTT               [GA]^AATT[CT]
AflIII        628  ACGTGT               A^C[GA][CT]GT
AgeI          266  ACCGGT               A^CCGGT
ApaI          378  GGGCCC               GGGCC^C
ApoI         1497  AAATTT               [GA]^AATT[CT]
Asp700I      1995  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       269  GGTACC               G^GTACC
AviII        1249  TGCGCA               TGC^GCA
BcgI          624  CGAGACGTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          362  TGATCA               T^GATCA
BsaAI         615  CACGTA               [CT]AC^GT[GA]
BsgI         2019  CTGCAC               GTGCAG(16/14)
BsmI         1777  GAATGC               GAATGC(1/-1)
BssSI         501  CACGAG               CACGAG(-5/-1)
BstXI         401  CCATGTGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI         1570  GACGCCTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1281  CCTATCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          362  TGATCA               T^GATCA
FspI         1249  TGCGCA               TGC^GCA
KpnI          269  GGTACC               GGTAC^C
NcoI         1667  CCATGG               C^CATGG
NdeI          284  CATATG               CA^TATG
NheI         1890  GCTAGC               G^CTAGC
PinAI         266  ACCGGT               A^CCGGT
SapI         1998  GCTCTTC              GCTCTTC(1/4)
SmaI         2108  CCCGGG               CCC^GGG
SphI         1052  GCATGC               GCATG^C
XmaCI        2108  CCCGGG               C^CCGGG
XmaI         2108  CCCGGG               C^CCGGG
XmnI         1995  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccIII        303  TCCGGA               T^CCGGA
             1685  TCCGGA              
AhdI          189  GACGCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1462  GACGGCCTGTC         
Aor51HI        53  AGCGCT               AGC^GCT
             2327  AGCGCT              
AspEI         189  GACGCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1462  GACGGCCTGTC         
BamHI        1787  GGATCC               G^GATCC
             1973  GGATCC              
BbsI          427  GTCTTC               GAAGAC(2/6)
              661  GTCTTC              
BfaI         1445  CTAG                 C^TAG
             1891  CTAG                
BpuAI         427  GTCTTC               GAAGAC(2/6)
              661  GTCTTC              
BsaI         1723  GAGACC               GGTCTC(1/5)
             2125  GAGACC              
BseAI         303  TCCGGA               T^CCGGA
             1685  TCCGGA              
BsiEI        1026  CGGCCG               CG[GA][CT]^CG
             2145  CGGCCG              
BsmBI         625  GAGACG               CGTCTC(1/5)
             2141  GAGACG              
BspDI         568  ATCGAT               AT^CGAT
              883  ATCGAT              
BspEI         303  TCCGGA               T^CCGGA
             1685  TCCGGA              
BsrBI        1023  GAGCGG               CCGCTC(-3/-3)
             1564  GAGCGG              
BstAPI       1772  GCATCGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2043  GCACCTGCTGC         
ClaI          568  ATCGAT               AT^CGAT
              883  ATCGAT              
DraIII       1110  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1708  CACTGTGTG           
DsaI         1667  CCATGG               C^C[GA][CT]GG
             2241  CCACGG              
EagI         1026  CGGCCG               C^GGCCG
             2145  CGGCCG              
Eam1105I      189  GACGCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1462  GACGGCCTGTC         
EclXI        1026  CGGCCG               C^GGCCG
             2145  CGGCCG              
Eco47III       53  AGCGCT               AGC^GCT
             2327  AGCGCT              
Eco52I       1026  CGGCCG               C^GGCCG
             2145  CGGCCG              
EcoT14I      1286  CCAAGG               C^C[AT][AT]GG
             1667  CCATGG              
MaeI         1445  CTAG                 C^TAG
             1891  CTAG                
MroI          303  TCCGGA               T^CCGGA
             1685  TCCGGA              
MseI          823  TTAA                 T^TAA
             1043  TTAA                
PflMI        1596  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1707  CCACTGTGTGG         
PpuMI         720  AGGACCC              [GA]G^G[AT]CC[CT]
             2069  AGGACCT             
PvuII          16  CAGCTG               CAG^CTG
              709  CAGCTG              
SacI          211  GAGCTC               GAGCT^C
             1855  GAGCTC              
StuI          993  AGGCCT               AGG^CCT
             2273  AGGCCT              
StyI         1286  CCAAGG               C^C[AT][AT]GG
             1667  CCATGG              
Tru9I         823  TTAA                 T^TAA
             1043  TTAA                
Van91I       1596  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1707  CCACTGTGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  101 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaHI       BsaJI       BsaWI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       
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