(Circular) (Six-base) MAPSORT of: pFN21ASDA1099.seq Check: 6361 from: 1 to: 7141 pFN21ASDA1099 7141 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2788 4077 2788 Size: 1289 5852 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4248 4951 278 Size: 53 83 186 936 2712 703 2468 Fragments arranged by size: 2712 2468 936 703 186 83 53 AccI GT'mk_AC Cuts at: 1937 3966 4484 1937 Size: 2029 518 4594 Fragments arranged by size: 4594 2029 518 Acc65I G'GTAC_C Cuts at: 2292 4462 2292 Size: 2170 4971 AclI AA'CG_TT Cuts at: 1563 5268 5641 1563 Size: 3705 373 3063 Fragments arranged by size: 3705 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2437 3525 5204 6588 6856 1632 Size: 805 1088 1679 1384 268 1917 Fragments arranged by size: 1917 1679 1384 1088 805 268 AfeI AGC'GCT Cuts at: 2078 4352 2078 Size: 2274 4867 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6944 AflIII A'CryG_T Cuts at: 1203 2651 6040 7017 1203 Size: 1448 3389 977 1327 Fragments arranged by size: 3389 1448 1327 977 AgeI A'CCGG_T Cuts at: 2289 5972 2289 Size: 3683 3458 AhdI GACnn_n'nnGTC Cuts at: 2217 3490 5870 2217 Size: 1273 2380 3488 Fragments arranged by size: 3488 2380 1273 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2820 4196 4337 1184 Size: 1636 1376 141 3988 Fragments arranged by size: 3988 1636 1376 141 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3981 4013 3981 Size: 32 7109 AlwNI CAG_nnn'CTG Cuts at: 2041 2734 3625 3927 4069 4225 4884 6456 2041 Size: 693 891 302 142 156 659 1572 2726 Fragments arranged by size: 2726 1572 891 693 659 302 156 142 ApaI G_GGCC'C Cuts at: 2405 2405 Size: 7141 ApaLI G'TGCA_C Cuts at: 2285 2635 2810 5198 6354 2285 Size: 350 175 2388 1156 3072 Fragments arranged by size: 3072 2388 1156 350 175 ApoI r'AATT_y Cuts at: 1493 3520 4450 4676 4828 6937 1493 Size: 2027 930 226 152 2109 1697 Fragments arranged by size: 2109 2027 1697 930 226 152 AseI AT'TA_AT Cuts at: 160 1032 5695 160 Size: 872 4663 1606 Fragments arranged by size: 4663 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 AvaI C'yCGr_G Cuts at: 1870 1943 2581 3255 3840 4131 4254 4466 1870 Size: 73 638 674 585 291 123 212 4545 Fragments arranged by size: 4545 674 638 585 291 212 123 73 BamHI G'GATC_C Cuts at: 3810 3996 4471 4838 3810 Size: 186 475 367 6113 Fragments arranged by size: 6113 475 367 186 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2292 3827 4420 4462 5917 Size: 325 217 627 505 1535 593 42 1455 Cuts at: 5917 618 Size: 1842 Fragments arranged by size: 1842 1535 1455 627 593 505 325 217 42 BanII G_rGCy'C Cuts at: 1391 2238 2405 3787 3882 4460 1391 Size: 847 167 1382 95 578 4072 Fragments arranged by size: 4072 1382 847 578 167 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2443 2677 928 Size: 971 544 234 5392 Fragments arranged by size: 5392 971 544 234 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2636 2670 5332 5366 1903 Size: 34 699 34 2662 34 3678 Fragments arranged by size: 3678 2662 699 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5033 6243 5033 Size: 1210 5931 BclI T'GATC_A Cuts at: 1548 2385 1548 Size: 837 6304 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2051 2063 3495 Size: 107 122 71 631 605 379 12 1432 Cuts at: 3495 4295 5752 136 Size: 800 1457 1525 Fragments arranged by size: 1525 1457 1432 800 631 605 379 122 107 71 12 BglII A'GATC_T Cuts at: 7136 7136 Size: 7141 BlpI GC'TnA_GC Cuts at: 4558 4558 Size: 7141 Bme1580I G_kGCm'C Cuts at: 1790 2289 2306 2405 2639 2814 4425 5202 6358 Size: 499 17 99 234 175 1611 777 1156 Cuts at: 6358 1790 Size: 2573 Fragments arranged by size: 2573 1611 1156 777 499 234 175 99 17 BmgBI CAC'GTC Cuts at: 1318 2170 2652 3264 4321 1318 Size: 852 482 612 1057 4138 Fragments arranged by size: 4138 1057 852 612 482 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3162 3375 3772 Size: 416 53 83 399 24 1745 213 397 Cuts at: 3772 4872 5830 442 Size: 1100 958 1753 Fragments arranged by size: 1753 1745 1100 958 416 399 397 213 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3917 1056 Size: 2861 4280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 3041 3268 3358 3373 4285 5801 1239 Size: 905 897 227 90 15 912 1516 2579 Fragments arranged by size: 2579 1516 912 905 897 227 90 15 Bpu10I CC'TnA_GC Cuts at: 3805 3805 Size: 7141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5266 6131 6429 5266 Size: 865 298 5978 Fragments arranged by size: 5978 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3740 4142 5804 882 Size: 617 2241 402 1662 2219 Fragments arranged by size: 2241 2219 1662 617 402 BsaAI yAC'GTr Cuts at: 493 1204 2640 4438 4932 493 Size: 711 1436 1798 494 2702 Fragments arranged by size: 2702 1798 1436 711 494 BsaBI GATnn'nnATC Cuts at: 7135 7135 Size: 7141 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2598 3594 4245 4948 Size: 53 83 186 936 1065 996 651 703 Cuts at: 4948 5330 5965 275 Size: 382 635 1451 Fragments arranged by size: 1451 1065 996 936 703 651 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2289 2326 3708 3816 4398 5574 5972 6246 6393 Size: 37 1382 108 582 1176 398 274 147 Cuts at: 6393 6804 2289 Size: 411 2626 Fragments arranged by size: 2626 1382 1176 582 411 398 274 147 108 37 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 2402 2432 2843 Size: 30 165 30 687 30 282 30 411 Cuts at: 2843 2873 4230 4260 1178 Size: 30 1357 30 4059 Fragments arranged by size: 4059 1357 687 411 282 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 2333 2851 3599 1186 Size: 964 183 518 748 4728 Fragments arranged by size: 4728 964 748 518 183 BseYI C'CCAG_C Cuts at: 2771 2879 3355 6344 6840 2771 Size: 108 476 2989 496 3072 Fragments arranged by size: 3072 2989 496 476 108 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4027 1163 Size: 573 2291 4277 Fragments arranged by size: 4277 2291 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 4171 4591 5352 6380 1540 Size: 275 186 1051 1119 420 761 1028 2301 Fragments arranged by size: 2301 1119 1051 1028 761 420 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 2238 2289 2639 2814 3882 4362 5202 5287 Size: 69 51 350 175 1068 480 840 85 Cuts at: 5287 6358 2169 Size: 1071 2952 Fragments arranged by size: 2952 1071 1068 840 480 350 175 85 69 51 BsmI GAATG_Cn' Cuts at: 3806 4652 4745 3806 Size: 846 93 6202 Fragments arranged by size: 6202 846 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2642 4158 2642 Size: 1516 5625 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2238 2289 2306 2405 2639 2814 Size: 399 379 69 51 17 99 234 175 Cuts at: 2814 3787 3882 4362 4425 4460 5202 5287 6358 Size: 973 95 480 63 35 742 85 1071 Cuts at: 6358 1391 Size: 2174 Fragments arranged by size: 2174 1071 973 742 480 399 379 234 175 99 95 85 69 63 51 35 17 BspEI T'CCGG_A Cuts at: 2326 3708 6804 2326 Size: 1382 3096 2663 Fragments arranged by size: 3096 2663 1382 BspHI T'CATG_A Cuts at: 5030 6760 5030 Size: 1730 5411 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2328 2788 4077 4104 4160 4496 844 Size: 1484 460 1289 27 56 336 3489 Fragments arranged by size: 3489 1484 1289 460 336 56 27 BsrBI CCG'CTC Cuts at: 1394 1823 3048 3589 5028 1394 Size: 429 1225 541 1439 3507 Fragments arranged by size: 3507 1439 1225 541 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5636 5810 65 Size: 1183 4388 174 1396 Fragments arranged by size: 4388 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2140 2289 2961 5785 5972 1700 Size: 110 330 149 672 2824 187 2869 Fragments arranged by size: 2869 2824 672 330 187 149 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7141 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7141 BssSI C'ACGA_G Cuts at: 2524 5201 6213 2524 Size: 2677 1012 3452 Fragments arranged by size: 3452 2677 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3801 4072 794 Size: 3007 271 3863 Fragments arranged by size: 3863 3007 271 BstBI TT'CG_AA Cuts at: 2007 5945 2007 Size: 3938 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7141 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2431 Size: 7141 BstYI r'GATC_y Cuts at: 1972 2070 2157 3621 3810 3996 4471 4838 5223 Size: 98 87 1464 189 186 475 367 385 Cuts at: 5223 5240 6692 6778 6790 7136 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1464 1452 475 385 367 346 189 186 98 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3690 4264 513 Size: 552 2625 574 3390 Fragments arranged by size: 3390 2625 574 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2158 487 Size: 683 56 932 5470 Fragments arranged by size: 5470 932 683 56 BtsI GCAGTG_nn' Cuts at: 2919 3498 4417 4657 5451 5471 2919 Size: 579 919 240 794 20 4589 Fragments arranged by size: 4589 919 794 579 240 20 ClaI AT'CG_AT Cuts at: 1554 2592 2907 1554 Size: 1038 315 5788 Fragments arranged by size: 5788 1038 315 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4393 4428 529 Size: 35 3829 35 3242 Fragments arranged by size: 3829 3242 35 35 DraI TTT'AAA Cuts at: 4445 4445 Size: 7141 DraIII CAC_nnn'GTG Cuts at: 3138 3736 3138 Size: 598 6543 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3599 6148 809 Size: 507 2283 2549 1802 Fragments arranged by size: 2549 2283 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2262 2508 Size: 54 1425 50 275 18 213 219 246 Cuts at: 2508 3049 3550 3627 3869 4104 4168 4365 4588 Size: 541 501 77 242 235 64 197 223 Cuts at: 4588 5477 7106 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 541 501 275 246 242 235 223 219 213 197 77 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 4168 4588 1537 Size: 275 1237 1119 420 4090 Fragments arranged by size: 4090 1237 1119 420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3239 3927 3975 4028 5071 1353 Size: 1886 688 48 53 1043 3423 Fragments arranged by size: 3423 1886 1043 688 53 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3964 5728 6102 6248 387 Size: 1064 138 2375 1764 374 146 1280 Fragments arranged by size: 2375 1764 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2236 3880 2236 Size: 1644 5497 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2437 3041 3268 3358 3373 3525 Size: 393 512 293 604 227 90 15 152 Cuts at: 3525 4285 5204 5801 6588 6856 1239 Size: 760 919 597 787 268 1524 Fragments arranged by size: 1524 919 787 760 604 597 512 393 293 268 227 152 90 15 EcoNI CCTnn'n_nnAGG Cuts at: 3308 3308 Size: 7141 EcoO109I rG'GnC_Cy Cuts at: 2401 2402 2744 4093 2401 Size: 1 342 1349 5449 Fragments arranged by size: 5449 1349 342 1 EcoRI G'AATT_C Cuts at: 4450 4828 4450 Size: 378 6763 FspI TGC'GCA Cuts at: 3274 5647 3274 Size: 2373 4768 HaeII r_GCGC'y Cuts at: 1678 2080 2962 4181 4354 6288 1678 Size: 402 882 1219 173 1934 2531 Fragments arranged by size: 2531 1934 1219 882 402 173 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2203 2235 2843 2875 3148 Size: 32 135 32 631 32 608 32 273 Cuts at: 3148 3180 3476 3508 3739 3771 4230 4262 5782 Size: 32 296 32 231 32 459 32 1520 Cuts at: 5782 5814 5856 5888 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1520 631 608 459 296 273 231 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4485 4731 669 Size: 988 281 2547 246 3079 Fragments arranged by size: 3079 2547 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7141 HpaI GTT'AAC Cuts at: 4731 4731 Size: 7141 KpnI G_GTAC'C Cuts at: 2296 4466 2296 Size: 2170 4971 MfeI C'AATT_G Cuts at: 4740 4740 Size: 7141 MluI A'CGCG_T Cuts at: 7017 7017 Size: 7141 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2643 2991 6255 6439 1305 Size: 1338 348 3264 184 2007 Fragments arranged by size: 3264 2007 1338 348 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 2264 2510 3552 3629 Size: 54 1425 343 213 219 246 1042 77 Cuts at: 3629 3871 4367 10 Size: 242 496 2784 Fragments arranged by size: 2784 1425 1042 496 343 246 242 219 213 77 54 MslI CAynn'nnrTG Cuts at: 518 1166 2429 3887 4319 5099 5458 5617 518 Size: 648 1263 1458 432 780 359 159 2042 Fragments arranged by size: 2042 1458 1263 780 648 432 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2268 2734 4036 4557 5234 6382 6627 Size: 435 227 466 1302 521 677 1148 245 Cuts at: 6627 1606 Size: 2120 Fragments arranged by size: 2120 1302 1148 677 521 466 435 245 227 NcoI C'CATG_G Cuts at: 513 1065 3690 513 Size: 552 2625 3964 Fragments arranged by size: 3964 2625 552 NdeI CA'TA_TG Cuts at: 387 2308 387 Size: 1921 5220 NheI G'CTAG_C Cuts at: 1052 3913 1052 Size: 2861 4280 NotI GC'GGCC_GC Cuts at: 4588 4588 Size: 7141 NspI r_CATG'y Cuts at: 1132 1229 3079 3187 3718 4499 6044 1132 Size: 97 1850 108 531 781 1545 2229 Fragments arranged by size: 2229 1850 1545 781 531 108 97 PasI CC'CwG_GG Cuts at: 1348 3239 4057 1348 Size: 1891 818 4432 Fragments arranged by size: 4432 1891 818 PciI A'CATG_T Cuts at: 6040 6040 Size: 7141 PflMI CCAn_nnn'nTGG Cuts at: 1108 3625 3736 1108 Size: 2517 111 4513 Fragments arranged by size: 4513 2517 111 PfoI T'CCnGG_A Cuts at: 2160 2229 2313 2688 4382 2160 Size: 69 84 375 1694 4919 Fragments arranged by size: 4919 1694 375 84 69 PmeI GTTT'AAAC Cuts at: 4445 4445 Size: 7141 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5210 5242 6723 6755 5210 Size: 32 1481 32 5596 Fragments arranged by size: 5596 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2744 4093 2744 Size: 1349 5792 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7141 PsiI TTA'TAA Cuts at: 4711 4711 Size: 7141 PspOMI G'GGCC_C Cuts at: 2401 2401 Size: 7141 PstI C_TGCA'G Cuts at: 830 2151 3639 3702 4332 4493 830 Size: 1321 1488 63 630 161 3478 Fragments arranged by size: 3478 1488 1321 630 161 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7141 PvuII CAG'CTG Cuts at: 2041 2734 2041 Size: 693 6448 SacI G_AGCT'C Cuts at: 2238 3882 2238 Size: 1644 5497 SalI G'TCGA_C Cuts at: 1936 4483 1936 Size: 2547 4594 SapI GCTCTTCn'nnn_ Cuts at: 4028 4028 Size: 7141 SbfI CC_TGCA'GG Cuts at: 4493 4493 Size: 7141 ScaI AGT'ACT Cuts at: 5389 7101 5389 Size: 1712 5429 SfcI C'TryA_G Cuts at: 826 1045 2147 2497 3635 3698 4328 4489 5624 Size: 219 1102 350 1138 63 630 161 1135 Cuts at: 5624 6305 6496 826 Size: 681 191 1471 Fragments arranged by size: 1471 1138 1135 1102 681 630 350 219 191 161 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7141 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7141 SmaI CCC'GGG Cuts at: 1872 4133 4468 1872 Size: 2261 335 4545 Fragments arranged by size: 4545 2261 335 SmlI C'TyrA_G Cuts at: 820 1017 1943 5245 6146 6408 820 Size: 197 926 3302 901 262 1553 Fragments arranged by size: 3302 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4438 4932 493 Size: 3945 494 2702 Fragments arranged by size: 3945 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7141 SphI G_CATG'C Cuts at: 1132 3079 4499 1132 Size: 1947 1420 3774 Fragments arranged by size: 3774 1947 1420 SspI AAT'ATT Cuts at: 5 52 5065 5 Size: 47 5013 2081 Fragments arranged by size: 5013 2081 47 StuI AGG'CCT Cuts at: 1517 3018 4298 1517 Size: 1501 1280 4360 Fragments arranged by size: 4360 1501 1280 StyI C'CwwG_G Cuts at: 513 1065 3309 3690 4580 513 Size: 552 2244 381 890 3074 Fragments arranged by size: 3074 2244 890 552 381 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2077 2419 3292 3347 3352 3845 5168 Size: 1255 497 342 873 55 5 493 1323 Cuts at: 5168 5185 5338 5523 5968 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1323 1255 873 497 493 445 342 185 153 55 17 5 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5387 7099 96 Size: 274 80 33 51 1443 3410 1712 138 Fragments arranged by size: 3410 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7101 7133 7101 Size: 32 7109 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7141 XbaI T'CTAG_A Cuts at: 4477 4477 Size: 7141 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2828 3775 3821 4114 2828 Size: 947 46 293 5855 Fragments arranged by size: 5855 947 293 46 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7141 XmaI C'CCGG_G Cuts at: 1870 4131 4466 1870 Size: 2261 335 4545 Fragments arranged by size: 4545 2261 335 XmnI GAAnn'nnTTC Cuts at: 4022 5270 4022 Size: 1248 5893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4246 4949 276 Size: 53 83 186 936 2712 703 2468 Fragments arranged by size: 2712 2468 936 703 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI