Restriction map for pFN21ASDA1099
 (Circular) (Six-base) MAPSORT of: pFN21ASDA1099.seq  Check: 6361  from: 1  to: 7141



pFN21ASDA1099  7141 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2788    4077    2788 
   Size:       1289    5852
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4248    4951     278 
   Size:         53      83     186     936    2712     703    2468

  Fragments arranged by size: 

                2712    2468     936     703     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3966    4484    1937 
   Size:       2029     518    4594

  Fragments arranged by size: 

                4594    2029     518
 
Acc65I G'GTAC_C

Cuts at:   2292    4462    2292 
   Size:       2170    4971
 
AclI AA'CG_TT

Cuts at:   1563    5268    5641    1563 
   Size:       3705     373    3063

  Fragments arranged by size: 

                3705    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2437    3525    5204    6588    6856    1632 
   Size:        805    1088    1679    1384     268    1917

  Fragments arranged by size: 

                1917    1679    1384    1088     805     268
 
AfeI AGC'GCT

Cuts at:   2078    4352    2078 
   Size:       2274    4867
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6944
 
AflIII A'CryG_T

Cuts at:   1203    2651    6040    7017    1203 
   Size:       1448    3389     977    1327

  Fragments arranged by size: 

                3389    1448    1327     977
 
AgeI A'CCGG_T

Cuts at:   2289    5972    2289 
   Size:       3683    3458
 
AhdI GACnn_n'nnGTC

Cuts at:   2217    3490    5870    2217 
   Size:       1273    2380    3488

  Fragments arranged by size: 

                3488    2380    1273
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       7141
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2820    4196    4337    1184 
   Size:       1636    1376     141    3988

  Fragments arranged by size: 

                3988    1636    1376     141
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3981    4013    3981 
   Size:         32    7109
 
AlwNI CAG_nnn'CTG

Cuts at:   2041    2734    3625    3927    4069    4225    4884    6456    2041 
   Size:        693     891     302     142     156     659    1572    2726

  Fragments arranged by size: 

                2726    1572     891     693     659     302     156     142
            
 
ApaI G_GGCC'C

Cuts at:   2405    2405 
   Size:       7141
 
ApaLI G'TGCA_C

Cuts at:   2285    2635    2810    5198    6354    2285 
   Size:        350     175    2388    1156    3072

  Fragments arranged by size: 

                3072    2388    1156     350     175
 
ApoI r'AATT_y

Cuts at:   1493    3520    4450    4676    4828    6937    1493 
   Size:       2027     930     226     152    2109    1697

  Fragments arranged by size: 

                2109    2027    1697     930     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    5695     160 
   Size:        872    4663    1606

  Fragments arranged by size: 

                4663    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7141
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2581    3255    3840    4131    4254    4466    1870 
   Size:         73     638     674     585     291     123     212    4545

  Fragments arranged by size: 

                4545     674     638     585     291     212     123      73
            
 
BamHI G'GATC_C

Cuts at:   3810    3996    4471    4838    3810 
   Size:        186     475     367    6113

  Fragments arranged by size: 

                6113     475     367     186
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2292    3827    4420    4462    5917 
   Size:        325     217     627     505    1535     593      42    1455

Cuts at:   5917     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1535    1455     627     593     505     325     217
                  42
 
BanII G_rGCy'C

Cuts at:   1391    2238    2405    3787    3882    4460    1391 
   Size:        847     167    1382      95     578    4072

  Fragments arranged by size: 

                4072    1382     847     578     167      95
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2443    2677     928 
   Size:        971     544     234    5392

  Fragments arranged by size: 

                5392     971     544     234
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2636    2670    5332    5366    1903 
   Size:         34     699      34    2662      34    3678

  Fragments arranged by size: 

                3678    2662     699      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   5033    6243    5033 
   Size:       1210    5931
 
BclI T'GATC_A

Cuts at:   1548    2385    1548 
   Size:        837    6304
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2051    2063    3495 
   Size:        107     122      71     631     605     379      12    1432

Cuts at:   3495    4295    5752     136 
   Size:        800    1457    1525

  Fragments arranged by size: 

                1525    1457    1432     800     631     605     379     122
                 107      71      12
 
BglII A'GATC_T

Cuts at:   7136    7136 
   Size:       7141
 
BlpI GC'TnA_GC

Cuts at:   4558    4558 
   Size:       7141
 
Bme1580I G_kGCm'C

Cuts at:   1790    2289    2306    2405    2639    2814    4425    5202    6358 
   Size:        499      17      99     234     175    1611     777    1156

Cuts at:   6358    1790 
   Size:       2573

  Fragments arranged by size: 

                2573    1611    1156     777     499     234     175      99
                  17
 
BmgBI CAC'GTC

Cuts at:   1318    2170    2652    3264    4321    1318 
   Size:        852     482     612    1057    4138

  Fragments arranged by size: 

                4138    1057     852     612     482
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3162    3375    3772 
   Size:        416      53      83     399      24    1745     213     397

Cuts at:   3772    4872    5830     442 
   Size:       1100     958    1753

  Fragments arranged by size: 

                1753    1745    1100     958     416     399     397     213
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    3917    1056 
   Size:       2861    4280
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2144    3041    3268    3358    3373    4285    5801    1239 
   Size:        905     897     227      90      15     912    1516    2579

  Fragments arranged by size: 

                2579    1516     912     905     897     227      90      15
            
 
Bpu10I CC'TnA_GC

Cuts at:   3805    3805 
   Size:       7141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   5266    6131    6429    5266 
   Size:        865     298    5978

  Fragments arranged by size: 

                5978     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3740    4142    5804     882 
   Size:        617    2241     402    1662    2219

  Fragments arranged by size: 

                2241    2219    1662     617     402
 
BsaAI yAC'GTr

Cuts at:    493    1204    2640    4438    4932     493 
   Size:        711    1436    1798     494    2702

  Fragments arranged by size: 

                2702    1798    1436     711     494
 
BsaBI GATnn'nnATC

Cuts at:   7135    7135 
   Size:       7141
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2598    3594    4245    4948 
   Size:         53      83     186     936    1065     996     651     703

Cuts at:   4948    5330    5965     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                1451    1065     996     936     703     651     635     382
                 186      83      53
 
BsaWI w'CCGG_w

Cuts at:   2289    2326    3708    3816    4398    5574    5972    6246    6393 
   Size:         37    1382     108     582    1176     398     274     147

Cuts at:   6393    6804    2289 
   Size:        411    2626

  Fragments arranged by size: 

                2626    1382    1176     582     411     398     274     147
                 108      37
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2090    2120    2402    2432    2843 
   Size:         30     165      30     687      30     282      30     411

Cuts at:   2843    2873    4230    4260    1178 
   Size:         30    1357      30    4059

  Fragments arranged by size: 

                4059    1357     687     411     282     165      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2150    2333    2851    3599    1186 
   Size:        964     183     518     748    4728

  Fragments arranged by size: 

                4728     964     748     518     183
 
BseYI C'CCAG_C

Cuts at:   2771    2879    3355    6344    6840    2771 
   Size:        108     476    2989     496    3072

  Fragments arranged by size: 

                3072    2989     496     476     108
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    4027    1163 
   Size:        573    2291    4277

  Fragments arranged by size: 

                4277    2291     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3052    4171    4591    5352    6380    1540 
   Size:        275     186    1051    1119     420     761    1028    2301

  Fragments arranged by size: 

                2301    1119    1051    1028     761     420     275     186
            
 
BsiHKAI G_wGCw'C

Cuts at:   2169    2238    2289    2639    2814    3882    4362    5202    5287 
   Size:         69      51     350     175    1068     480     840      85

Cuts at:   5287    6358    2169 
   Size:       1071    2952

  Fragments arranged by size: 

                2952    1071    1068     840     480     350     175      85
                  69      51
 
BsmI GAATG_Cn'

Cuts at:   3806    4652    4745    3806 
   Size:        846      93    6202

  Fragments arranged by size: 

                6202     846      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2642    4158    2642 
   Size:       1516    5625
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2169    2238    2289    2306    2405    2639    2814 
   Size:        399     379      69      51      17      99     234     175

Cuts at:   2814    3787    3882    4362    4425    4460    5202    5287    6358 
   Size:        973      95     480      63      35     742      85    1071

Cuts at:   6358    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     973     742     480     399     379     234
                 175      99      95      85      69      63      51      35
                  17
 
BspEI T'CCGG_A

Cuts at:   2326    3708    6804    2326 
   Size:       1382    3096    2663

  Fragments arranged by size: 

                3096    2663    1382
 
BspHI T'CATG_A

Cuts at:   5030    6760    5030 
   Size:       1730    5411
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2328    2788    4077    4104    4160    4496     844 
   Size:       1484     460    1289      27      56     336    3489

  Fragments arranged by size: 

                3489    1484    1289     460     336      56      27
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3048    3589    5028    1394 
   Size:        429    1225     541    1439    3507

  Fragments arranged by size: 

                3507    1439    1225     541     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    5636    5810      65 
   Size:       1183    4388     174    1396

  Fragments arranged by size: 

                4388    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2140    2289    2961    5785    5972    1700 
   Size:        110     330     149     672    2824     187    2869

  Fragments arranged by size: 

                2869    2824     672     330     187     149     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       7141
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7141
 
BssSI C'ACGA_G

Cuts at:   2524    5201    6213    2524 
   Size:       2677    1012    3452

  Fragments arranged by size: 

                3452    2677    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3801    4072     794 
   Size:       3007     271    3863

  Fragments arranged by size: 

                3863    3007     271
 
BstBI TT'CG_AA

Cuts at:   2007    5945    2007 
   Size:       3938    3203
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       7141
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2431    2431 
   Size:       7141
 
BstYI r'GATC_y

Cuts at:   1972    2070    2157    3621    3810    3996    4471    4838    5223 
   Size:         98      87    1464     189     186     475     367     385

Cuts at:   5223    5240    6692    6778    6790    7136    1972 
   Size:         17    1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1464    1452     475     385     367     346     189
                 186      98      87      86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    3690    4264     513 
   Size:        552    2625     574    3390

  Fragments arranged by size: 

                3390    2625     574     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2158     487 
   Size:        683      56     932    5470

  Fragments arranged by size: 

                5470     932     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2919    3498    4417    4657    5451    5471    2919 
   Size:        579     919     240     794      20    4589

  Fragments arranged by size: 

                4589     919     794     579     240      20
 
ClaI AT'CG_AT

Cuts at:   1554    2592    2907    1554 
   Size:       1038     315    5788

  Fragments arranged by size: 

                5788    1038     315
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    4393    4428     529 
   Size:         35    3829      35    3242

  Fragments arranged by size: 

                3829    3242      35      35
 
DraI TTT'AAA

Cuts at:   4445    4445 
   Size:       7141
 
DraIII CAC_nnn'GTG

Cuts at:   3138    3736    3138 
   Size:        598    6543
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3599    6148     809 
   Size:        507    2283    2549    1802

  Fragments arranged by size: 

                2549    2283    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2043    2262    2508 
   Size:         54    1425      50     275      18     213     219     246

Cuts at:   2508    3049    3550    3627    3869    4104    4168    4365    4588 
   Size:        541     501      77     242     235      64     197     223

Cuts at:   4588    5477    7106       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     541     501     275     246     242
                 235     223     219     213     197      77      64      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3049    4168    4588    1537 
   Size:        275    1237    1119     420    4090

  Fragments arranged by size: 

                4090    1237    1119     420     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3239    3927    3975    4028    5071    1353 
   Size:       1886     688      48      53    1043    3423

  Fragments arranged by size: 

                3423    1886    1043     688      53      48
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3964    5728    6102    6248     387 
   Size:       1064     138    2375    1764     374     146    1280

  Fragments arranged by size: 

                2375    1764    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2236    3880    2236 
   Size:       1644    5497
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2144    2437    3041    3268    3358    3373    3525 
   Size:        393     512     293     604     227      90      15     152

Cuts at:   3525    4285    5204    5801    6588    6856    1239 
   Size:        760     919     597     787     268    1524

  Fragments arranged by size: 

                1524     919     787     760     604     597     512     393
                 293     268     227     152      90      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3308    3308 
   Size:       7141
 
EcoO109I rG'GnC_Cy

Cuts at:   2401    2402    2744    4093    2401 
   Size:          1     342    1349    5449

  Fragments arranged by size: 

                5449    1349     342       1
 
EcoRI G'AATT_C

Cuts at:   4450    4828    4450 
   Size:        378    6763
 
FspI TGC'GCA

Cuts at:   3274    5647    3274 
   Size:       2373    4768
 
HaeII r_GCGC'y

Cuts at:   1678    2080    2962    4181    4354    6288    1678 
   Size:        402     882    1219     173    1934    2531

  Fragments arranged by size: 

                2531    1934    1219     882     402     173
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2203    2235    2843    2875    3148 
   Size:         32     135      32     631      32     608      32     273

Cuts at:   3148    3180    3476    3508    3739    3771    4230    4262    5782 
   Size:         32     296      32     231      32     459      32    1520

Cuts at:   5782    5814    5856    5888    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1520     631     608     459     296     273     231
                 135      42      32      32      32      32      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    4485    4731     669 
   Size:        988     281    2547     246    3079

  Fragments arranged by size: 

                3079    2547     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       7141
 
HpaI GTT'AAC

Cuts at:   4731    4731 
   Size:       7141
 
KpnI G_GTAC'C

Cuts at:   2296    4466    2296 
   Size:       2170    4971
 
MfeI C'AATT_G

Cuts at:   4740    4740 
   Size:       7141
 
MluI A'CGCG_T

Cuts at:   7017    7017 
   Size:       7141
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2643    2991    6255    6439    1305 
   Size:       1338     348    3264     184    2007

  Fragments arranged by size: 

                3264    2007    1338     348     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2045    2264    2510    3552    3629 
   Size:         54    1425     343     213     219     246    1042      77

Cuts at:   3629    3871    4367      10 
   Size:        242     496    2784

  Fragments arranged by size: 

                2784    1425    1042     496     343     246     242     219
                 213      77      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2429    3887    4319    5099    5458    5617     518 
   Size:        648    1263    1458     432     780     359     159    2042

  Fragments arranged by size: 

                2042    1458    1263     780     648     432     359     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    2041    2268    2734    4036    4557    5234    6382    6627 
   Size:        435     227     466    1302     521     677    1148     245

Cuts at:   6627    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1302    1148     677     521     466     435     245
                 227
 
NcoI C'CATG_G

Cuts at:    513    1065    3690     513 
   Size:        552    2625    3964

  Fragments arranged by size: 

                3964    2625     552
 
NdeI CA'TA_TG

Cuts at:    387    2308     387 
   Size:       1921    5220
 
NheI G'CTAG_C

Cuts at:   1052    3913    1052 
   Size:       2861    4280
 
NotI GC'GGCC_GC

Cuts at:   4588    4588 
   Size:       7141
 
NspI r_CATG'y

Cuts at:   1132    1229    3079    3187    3718    4499    6044    1132 
   Size:         97    1850     108     531     781    1545    2229

  Fragments arranged by size: 

                2229    1850    1545     781     531     108      97
 
PasI CC'CwG_GG

Cuts at:   1348    3239    4057    1348 
   Size:       1891     818    4432

  Fragments arranged by size: 

                4432    1891     818
 
PciI A'CATG_T

Cuts at:   6040    6040 
   Size:       7141
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3625    3736    1108 
   Size:       2517     111    4513

  Fragments arranged by size: 

                4513    2517     111
 
PfoI T'CCnGG_A

Cuts at:   2160    2229    2313    2688    4382    2160 
   Size:         69      84     375    1694    4919

  Fragments arranged by size: 

                4919    1694     375      84      69
 
PmeI GTTT'AAAC

Cuts at:   4445    4445 
   Size:       7141
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5210    5242    6723    6755    5210 
   Size:         32    1481      32    5596

  Fragments arranged by size: 

                5596    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2744    4093    2744 
   Size:       1349    5792
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       7141
 
PsiI TTA'TAA

Cuts at:   4711    4711 
   Size:       7141
 
PspOMI G'GGCC_C

Cuts at:   2401    2401 
   Size:       7141
 
PstI C_TGCA'G

Cuts at:    830    2151    3639    3702    4332    4493     830 
   Size:       1321    1488      63     630     161    3478

  Fragments arranged by size: 

                3478    1488    1321     630     161      63
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7141
 
PvuII CAG'CTG

Cuts at:   2041    2734    2041 
   Size:        693    6448
 
SacI G_AGCT'C

Cuts at:   2238    3882    2238 
   Size:       1644    5497
 
SalI G'TCGA_C

Cuts at:   1936    4483    1936 
   Size:       2547    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   4028    4028 
   Size:       7141
 
SbfI CC_TGCA'GG

Cuts at:   4493    4493 
   Size:       7141
 
ScaI AGT'ACT

Cuts at:   5389    7101    5389 
   Size:       1712    5429
 
SfcI C'TryA_G

Cuts at:    826    1045    2147    2497    3635    3698    4328    4489    5624 
   Size:        219    1102     350    1138      63     630     161    1135

Cuts at:   5624    6305    6496     826 
   Size:        681     191    1471

  Fragments arranged by size: 

                1471    1138    1135    1102     681     630     350     219
                 191     161      63
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7141
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7141
 
SmaI CCC'GGG

Cuts at:   1872    4133    4468    1872 
   Size:       2261     335    4545

  Fragments arranged by size: 

                4545    2261     335
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    5245    6146    6408     820 
   Size:        197     926    3302     901     262    1553

  Fragments arranged by size: 

                3302    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4438    4932     493 
   Size:       3945     494    2702

  Fragments arranged by size: 

                3945    2702     494
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7141
 
SphI G_CATG'C

Cuts at:   1132    3079    4499    1132 
   Size:       1947    1420    3774

  Fragments arranged by size: 

                3774    1947    1420
 
SspI AAT'ATT

Cuts at:      5      52    5065       5 
   Size:         47    5013    2081

  Fragments arranged by size: 

                5013    2081      47
 
StuI AGG'CCT

Cuts at:   1517    3018    4298    1517 
   Size:       1501    1280    4360

  Fragments arranged by size: 

                4360    1501    1280
 
StyI C'CwwG_G

Cuts at:    513    1065    3309    3690    4580     513 
   Size:        552    2244     381     890    3074

  Fragments arranged by size: 

                3074    2244     890     552     381
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2077    2419    3292    3347    3352    3845    5168 
   Size:       1255     497     342     873      55       5     493    1323

Cuts at:   5168    5185    5338    5523    5968     325 
   Size:         17     153     185     445    1498

  Fragments arranged by size: 

                1498    1323    1255     873     497     493     445     342
                 185     153      55      17       5
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    5387    7099      96 
   Size:        274      80      33      51    1443    3410    1712     138

  Fragments arranged by size: 

                3410    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   7101    7133    7101 
   Size:         32    7109
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7141
 
XbaI T'CTAG_A

Cuts at:   4477    4477 
   Size:       7141
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2828    3775    3821    4114    2828 
   Size:        947      46     293    5855

  Fragments arranged by size: 

                5855     947     293      46
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7141
 
XmaI C'CCGG_G

Cuts at:   1870    4131    4466    1870 
   Size:       2261     335    4545

  Fragments arranged by size: 

                4545    2261     335
 
XmnI GAAnn'nnTTC

Cuts at:   4022    5270    4022 
   Size:       1248    5893
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4246    4949     276 
   Size:         53      83     186     936    2712     703    2468

  Fragments arranged by size: 

                2712    2468     936     703     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI
     PvuI    PvuII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI     BplI    BsiWI
  BstZ17I   Bsu36I    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PspXI     PsrI
    RsrII    SacII    SanDI    SexAI     SfiI     SfoI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com