(Circular) (Six-base) MAPSORT of: pFN21ASDA1083.seq Check: 1187 from: 1 to: 6481 pFN21ASDA1083 6481 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2326 2326 Size: 6481 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4291 278 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3301 3824 1937 Size: 1364 523 4594 Fragments arranged by size: 4594 1364 523 Acc65I G'GTAC_C Cuts at: 3802 3802 Size: 6481 AclI AA'CG_TT Cuts at: 1563 4608 4981 1563 Size: 3045 373 3063 Fragments arranged by size: 3063 3045 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4544 5928 6196 1632 Size: 2912 1384 268 1917 Fragments arranged by size: 2912 1917 1384 268 AfeI AGC'GCT Cuts at: 2264 2264 Size: 6481 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6284 AflIII A'CryG_T Cuts at: 1203 5380 6357 1203 Size: 4177 977 1327 Fragments arranged by size: 4177 1327 977 AgeI A'CCGG_T Cuts at: 5312 5312 Size: 6481 AhdI GACnn_n'nnGTC Cuts at: 5210 5210 Size: 6481 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6481 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2096 1184 Size: 912 5569 AlwNI CAG_nnn'CTG Cuts at: 2318 3113 4224 5796 2318 Size: 795 1111 1572 3003 Fragments arranged by size: 3003 1572 1111 795 ApaLI G'TGCA_C Cuts at: 4538 5694 4538 Size: 1156 5325 ApoI r'AATT_y Cuts at: 1493 2026 2872 2945 3317 3329 3790 4016 4168 Size: 533 846 73 372 12 461 226 152 Cuts at: 4168 6277 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 846 533 461 372 226 152 73 12 AseI AT'TA_AT Cuts at: 160 1032 5035 160 Size: 872 4003 1606 Fragments arranged by size: 4003 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 AvaI C'yCGr_G Cuts at: 1870 1943 2147 3216 3806 1870 Size: 73 204 1069 590 4545 Fragments arranged by size: 4545 1069 590 204 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3756 3789 3756 Size: 33 6448 BamHI G'GATC_C Cuts at: 3811 4178 3811 Size: 367 6114 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2080 2338 2344 3802 Size: 325 217 627 279 14 258 6 1458 Cuts at: 3802 5257 618 Size: 1455 1842 Fragments arranged by size: 1842 1458 1455 627 325 279 258 217 14 6 BanII G_rGCy'C Cuts at: 1391 2241 2300 3046 3202 3800 1391 Size: 850 59 746 156 598 4072 Fragments arranged by size: 4072 850 746 598 156 59 BbeI G_GCGC'C Cuts at: 2070 2342 2070 Size: 272 6209 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2368 3095 928 Size: 971 469 727 4314 Fragments arranged by size: 4314 971 727 469 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2304 2338 4672 4706 1903 Size: 34 367 34 2334 34 3678 Fragments arranged by size: 3678 2334 367 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4373 5583 3548 Size: 825 1210 4446 Fragments arranged by size: 4446 1210 825 BclI T'GATC_A Cuts at: 1548 1548 Size: 6481 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2089 2135 2282 Size: 107 122 71 631 605 417 46 147 Cuts at: 2282 2709 5092 136 Size: 427 2383 1525 Fragments arranged by size: 2383 1525 631 605 427 417 147 122 107 71 46 BglII A'GATC_T Cuts at: 6476 6476 Size: 6481 BlpI GC'TnA_GC Cuts at: 3898 3898 Size: 6481 Bme1580I G_kGCm'C Cuts at: 1790 4542 5698 1790 Size: 2752 1156 2573 Fragments arranged by size: 2752 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6481 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3608 4212 5170 Size: 416 53 83 399 24 2191 604 958 Cuts at: 5170 442 Size: 1753 Fragments arranged by size: 2191 1753 958 604 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6481 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2419 2419 Size: 6481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2775 3372 5141 1239 Size: 1536 597 1769 2579 Fragments arranged by size: 2579 1769 1536 597 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4606 5471 5769 4606 Size: 865 298 5318 Fragments arranged by size: 5318 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5144 882 Size: 617 3645 2219 Fragments arranged by size: 3645 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3172 3778 4272 493 Size: 711 1968 606 494 2702 Fragments arranged by size: 2702 1968 711 606 494 BsaBI GATnn'nnATC Cuts at: 6475 6475 Size: 6481 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2339 2548 3304 Size: 53 83 186 936 534 272 209 756 Cuts at: 3304 4288 4670 5305 275 Size: 984 382 635 1451 Fragments arranged by size: 1451 984 936 756 635 534 382 272 209 186 83 53 BsaWI w'CCGG_w Cuts at: 4914 5312 5586 5733 6144 4914 Size: 398 274 147 411 5251 Fragments arranged by size: 5251 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 1178 Size: 30 165 30 762 30 5464 Fragments arranged by size: 5464 762 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2308 3473 1186 Size: 1045 77 1165 4194 Fragments arranged by size: 4194 1165 1045 77 BseYI C'CCAG_C Cuts at: 5684 6180 5684 Size: 496 5985 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5908 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3931 4692 5720 1540 Size: 275 186 1930 761 1028 2301 Fragments arranged by size: 2301 1930 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2300 2725 3046 3291 4542 4627 5698 2300 Size: 425 321 245 1251 85 1071 3083 Fragments arranged by size: 3083 1251 1071 425 321 245 85 BsmI GAATG_Cn' Cuts at: 3130 3992 4085 3130 Size: 862 93 5526 Fragments arranged by size: 5526 862 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2300 2725 3046 3202 3291 3800 Size: 399 451 59 425 321 156 89 509 Cuts at: 3800 4542 4627 5698 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 509 451 425 399 321 156 89 85 59 BspEI T'CCGG_A Cuts at: 6144 6144 Size: 6481 BspHI T'CATG_A Cuts at: 4370 6100 4370 Size: 1730 4751 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2326 3836 844 Size: 1482 1510 3489 Fragments arranged by size: 3489 1510 1482 BsrBI CCG'CTC Cuts at: 1394 1823 4368 1394 Size: 429 2545 3507 Fragments arranged by size: 3507 2545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2406 2984 4976 5150 65 Size: 1183 1158 578 1992 174 1396 Fragments arranged by size: 1992 1396 1183 1158 578 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2134 2341 5125 5312 1700 Size: 110 324 207 2784 187 2869 Fragments arranged by size: 2869 2784 324 207 187 110 BsrGI T'GTAC_A Cuts at: 96 3340 96 Size: 3244 3237 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6481 BssSI C'ACGA_G Cuts at: 4541 5553 4541 Size: 1012 5469 BstAPI GCAn_nnn'nTGC Cuts at: 794 3113 3536 3590 794 Size: 2319 423 54 3685 Fragments arranged by size: 3685 2319 423 54 BstBI TT'CG_AA Cuts at: 2007 5285 2007 Size: 3278 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6481 BstYI r'GATC_y Cuts at: 1972 2693 2768 3811 4178 4563 4580 6032 6118 Size: 721 75 1043 367 385 17 1452 86 Cuts at: 6118 6130 6476 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1043 721 385 367 346 86 75 17 12 BtgI C'CryG_G Cuts at: 513 1065 2745 513 Size: 552 1680 4249 Fragments arranged by size: 4249 1680 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5742 Fragments arranged by size: 5742 683 56 BtsI GCAGTG_nn' Cuts at: 2660 3997 4791 4811 2660 Size: 1337 794 20 4330 Fragments arranged by size: 4330 1337 794 20 ClaI AT'CG_AT Cuts at: 1554 2421 1554 Size: 867 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3153 3188 529 Size: 35 2589 35 3822 Fragments arranged by size: 3822 2589 35 35 DraI TTT'AAA Cuts at: 3785 3785 Size: 6481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5488 809 Size: 507 4172 1802 Fragments arranged by size: 4172 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2583 3928 4817 Size: 54 1425 50 275 18 753 1345 889 Cuts at: 4817 6446 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1345 889 753 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3928 1537 Size: 275 2116 4090 Fragments arranged by size: 4090 2116 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 3271 4411 1353 Size: 896 1022 1140 3423 Fragments arranged by size: 3423 1140 1022 896 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2085 2133 5068 5442 5588 387 Size: 1064 138 496 48 2935 374 146 1280 Fragments arranged by size: 2935 1280 1064 496 374 146 138 48 EcoICRI GAG'CTC Cuts at: 2298 3044 2298 Size: 746 5735 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2775 3372 4544 5141 5928 6196 1239 Size: 393 1143 597 1172 597 787 268 1524 Fragments arranged by size: 1524 1172 1143 787 597 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3051 3051 Size: 6481 EcoO109I rG'GnC_Cy Cuts at: 3602 3602 Size: 6481 EcoRI G'AATT_C Cuts at: 2026 3790 4168 2026 Size: 1764 378 4339 Fragments arranged by size: 4339 1764 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2611 3177 2611 Size: 566 5915 FseI GG_CCGG'CC Cuts at: 2138 2138 Size: 6481 FspI TGC'GCA Cuts at: 2417 4987 2417 Size: 2570 3911 HaeII r_GCGC'y Cuts at: 1678 2070 2266 2342 5628 1678 Size: 392 196 76 3286 2531 Fragments arranged by size: 3286 2531 392 196 76 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3542 3574 5122 5154 5196 Size: 32 135 32 1970 32 1548 32 42 Cuts at: 5196 5228 1373 Size: 32 2626 Fragments arranged by size: 2626 1970 1548 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3825 4071 669 Size: 988 281 1887 246 3079 Fragments arranged by size: 3079 1887 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6481 HpaI GTT'AAC Cuts at: 4071 4071 Size: 6481 KasI G'GCGC_C Cuts at: 2066 2338 2066 Size: 272 6209 KpnI G_GTAC'C Cuts at: 3806 3806 Size: 6481 MfeI C'AATT_G Cuts at: 4080 4080 Size: 6481 MluI A'CGCG_T Cuts at: 6357 6357 Size: 6481 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3725 5595 5779 1305 Size: 2420 1870 184 2007 Fragments arranged by size: 2420 2007 1870 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2585 10 Size: 54 1425 343 753 3906 Fragments arranged by size: 3906 1425 753 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4439 4798 4957 518 Size: 648 3273 359 159 2042 Fragments arranged by size: 3273 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2939 3897 4574 5722 5967 1606 Size: 585 748 958 677 1148 245 2120 Fragments arranged by size: 2120 1148 958 748 677 585 245 NaeI GCC'GGC Cuts at: 2136 2136 Size: 6481 NarI GG'CG_CC Cuts at: 2067 2339 2067 Size: 272 6209 NcoI C'CATG_G Cuts at: 513 1065 2745 513 Size: 552 1680 4249 Fragments arranged by size: 4249 1680 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6481 NgoMIV G'CCGG_C Cuts at: 2134 2134 Size: 6481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6481 NotI GC'GGCC_GC Cuts at: 3928 3928 Size: 6481 NspI r_CATG'y Cuts at: 1132 1229 3839 5384 1132 Size: 97 2610 1545 2229 Fragments arranged by size: 2610 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6481 PciI A'CATG_T Cuts at: 5380 5380 Size: 6481 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6481 PmeI GTTT'AAAC Cuts at: 3785 3785 Size: 6481 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4550 4582 6063 6095 4550 Size: 32 1481 32 4936 Fragments arranged by size: 4936 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3602 3602 Size: 6481 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6481 PsiI TTA'TAA Cuts at: 2909 4051 2909 Size: 1142 5339 PstI C_TGCA'G Cuts at: 830 2701 3118 3833 830 Size: 1871 417 715 3478 Fragments arranged by size: 3478 1871 715 417 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6481 PvuII CAG'CTG Cuts at: 2939 2939 Size: 6481 SacI G_AGCT'C Cuts at: 2300 3046 2300 Size: 746 5735 SalI G'TCGA_C Cuts at: 1936 3823 1936 Size: 1887 4594 SbfI CC_TGCA'GG Cuts at: 3833 3833 Size: 6481 ScaI AGT'ACT Cuts at: 3366 4729 6441 3366 Size: 1363 1712 3406 Fragments arranged by size: 3406 1712 1363 SfcI C'TryA_G Cuts at: 826 1045 2697 3114 3829 4964 5645 5836 826 Size: 219 1652 417 715 1135 681 191 1471 Fragments arranged by size: 1652 1471 1135 715 681 417 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2709 2709 Size: 6481 SfoI GGC'GCC Cuts at: 2068 2340 2068 Size: 272 6209 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6481 SgrAI Cr'CCGG_yG Cuts at: 1700 2341 1700 Size: 641 5840 SmaI CCC'GGG Cuts at: 1872 3808 1872 Size: 1936 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3216 4585 5486 5748 820 Size: 197 926 1273 1369 901 262 1553 Fragments arranged by size: 1553 1369 1273 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3778 4272 493 Size: 3285 494 2702 Fragments arranged by size: 3285 2702 494 SpeI A'CTAG_T Cuts at: 152 2651 152 Size: 2499 3982 SphI G_CATG'C Cuts at: 1132 3839 1132 Size: 2707 3774 SspI AAT'ATT Cuts at: 5 52 3664 4405 5 Size: 47 3612 741 2081 Fragments arranged by size: 3612 2081 741 47 StuI AGG'CCT Cuts at: 1517 2092 2390 2703 3019 1517 Size: 575 298 313 316 4979 Fragments arranged by size: 4979 575 316 313 298 StyI C'CwwG_G Cuts at: 513 1065 2586 2745 3920 513 Size: 552 1521 159 1175 3074 Fragments arranged by size: 3074 1521 1175 552 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3365 4508 4525 4678 4863 5308 325 Size: 1255 1785 1143 17 153 185 445 1498 Fragments arranged by size: 1785 1498 1255 1143 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 2397 3340 Size: 274 80 33 51 1443 195 225 943 Cuts at: 3340 3364 4727 6439 96 Size: 24 1363 1712 138 Fragments arranged by size: 1712 1443 1363 943 274 225 195 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6441 6473 6441 Size: 32 6449 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6481 XbaI T'CTAG_A Cuts at: 2605 3817 2605 Size: 1212 5269 XhoI C'TCGA_G Cuts at: 1943 3216 1943 Size: 1273 5208 XmaI C'CCGG_G Cuts at: 1870 3806 1870 Size: 1936 4545 XmnI GAAnn'nnTTC Cuts at: 4610 4610 Size: 6481 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4289 276 Size: 53 83 186 936 2755 2468 Fragments arranged by size: 2755 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI XcmI