(Circular) (Six-base) MAPSORT of: pFN21ASDA1075.seq Check: 4608 from: 1 to: 8956 pFN21ASDA1075 8956 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2502 3194 5194 5265 2502 Size: 692 2000 71 6193 Fragments arranged by size: 6193 2000 692 71 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6766 278 Size: 53 83 186 936 5230 2468 Fragments arranged by size: 5230 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3024 3348 6299 1937 Size: 1087 324 2951 4594 Fragments arranged by size: 4594 2951 1087 324 Acc65I G'GTAC_C Cuts at: 4368 4590 6277 4368 Size: 222 1687 7047 Fragments arranged by size: 7047 1687 222 AclI AA'CG_TT Cuts at: 1563 7083 7456 1563 Size: 5520 373 3063 Fragments arranged by size: 5520 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2235 2362 2866 4307 5504 7019 8403 8671 Size: 603 127 504 1441 1197 1515 1384 268 Cuts at: 8671 1632 Size: 1917 Fragments arranged by size: 1917 1515 1441 1384 1197 603 504 268 127 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8759 AflIII A'CryG_T Cuts at: 1203 3122 7855 8832 1203 Size: 1919 4733 977 1327 Fragments arranged by size: 4733 1919 1327 977 AgeI A'CCGG_T Cuts at: 7787 7787 Size: 8956 AhdI GACnn_n'nnGTC Cuts at: 4810 7685 4810 Size: 2875 6081 AleI CACnn'nnGTG Cuts at: 1166 2426 3135 5123 1166 Size: 1260 709 1988 4999 Fragments arranged by size: 4999 1988 1260 709 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2381 4275 1184 Size: 1197 1894 5865 Fragments arranged by size: 5865 1894 1197 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4389 4421 5601 5633 4389 Size: 32 1180 32 7712 Fragments arranged by size: 7712 1180 32 32 AlwNI CAG_nnn'CTG Cuts at: 2208 2797 3056 3629 3643 4010 4416 4840 4977 Size: 589 259 573 14 367 406 424 137 Cuts at: 4977 6699 8271 2208 Size: 1722 1572 2893 Fragments arranged by size: 2893 1722 1572 589 573 424 406 367 259 137 14 ApaI G_GGCC'C Cuts at: 4673 5294 5646 5897 4673 Size: 621 352 251 7732 Fragments arranged by size: 7732 621 352 251 ApaLI G'TGCA_C Cuts at: 7013 8169 7013 Size: 1156 7800 ApoI r'AATT_y Cuts at: 1493 2819 6265 6491 6643 8752 1493 Size: 1326 3446 226 152 2109 1697 Fragments arranged by size: 3446 2109 1697 1326 226 152 AseI AT'TA_AT Cuts at: 160 1032 7510 160 Size: 872 6478 1606 Fragments arranged by size: 6478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8956 AvaI C'yCGr_G Cuts at: 1870 1943 3299 3444 4519 4704 5082 5261 5910 Size: 73 1356 145 1075 185 378 179 649 Cuts at: 5910 6281 1870 Size: 371 4545 Fragments arranged by size: 4545 1356 1075 649 378 371 185 179 145 73 AvrII C'CTAG_G Cuts at: 2099 3655 2099 Size: 1556 7400 BamHI G'GATC_C Cuts at: 2324 3905 6286 6653 2324 Size: 1581 2381 367 4627 Fragments arranged by size: 4627 2381 1581 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2481 3508 3945 4218 4368 Size: 325 217 627 694 1027 437 273 150 Cuts at: 4368 4487 4590 4605 4880 5015 6203 6277 7732 Size: 119 103 15 275 135 1188 74 1455 Cuts at: 7732 618 Size: 1842 Fragments arranged by size: 1842 1455 1188 1027 694 627 437 325 275 273 217 150 135 119 103 74 15 BanII G_rGCy'C Cuts at: 1391 2062 3825 4673 5033 5177 5294 5646 5682 Size: 671 1763 848 360 144 117 352 36 Cuts at: 5682 5897 6275 1391 Size: 215 378 4072 Fragments arranged by size: 4072 1763 848 671 378 360 352 215 144 117 36 BbeI G_GCGC'C Cuts at: 3949 5019 6207 3949 Size: 1070 1188 6698 Fragments arranged by size: 6698 1188 1070 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3277 928 Size: 971 1378 6607 Fragments arranged by size: 6607 1378 971 BbvCI CC'TCA_GC Cuts at: 3785 4615 4822 3785 Size: 830 207 7919 Fragments arranged by size: 7919 830 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5063 5097 7147 7181 1903 Size: 34 3126 34 2050 34 3678 Fragments arranged by size: 3678 3126 2050 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3774 4485 5419 6848 8058 3774 Size: 711 934 1429 1210 4672 Fragments arranged by size: 4672 1429 1210 934 711 BclI T'GATC_A Cuts at: 1548 5511 1548 Size: 3963 4993 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2261 3493 3810 Size: 107 122 71 631 605 589 1232 317 Cuts at: 3810 4347 5085 7567 136 Size: 537 738 2482 1525 Fragments arranged by size: 2482 1525 1232 738 631 605 589 537 317 122 107 71 BglII A'GATC_T Cuts at: 2772 6114 8951 2772 Size: 3342 2837 2777 Fragments arranged by size: 3342 2837 2777 BlpI GC'TnA_GC Cuts at: 2109 4553 6373 2109 Size: 2444 1820 4692 Fragments arranged by size: 4692 2444 1820 Bme1580I G_kGCm'C Cuts at: 1790 3149 4221 4317 4343 4492 4608 4673 4683 Size: 1359 1072 96 26 149 116 65 10 Cuts at: 4683 5040 5294 5584 5646 5802 5897 7017 8173 Size: 357 254 290 62 156 95 1120 1156 Cuts at: 8173 1790 Size: 2573 Fragments arranged by size: 2573 1359 1156 1120 1072 357 290 254 156 149 116 96 95 65 62 26 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8956 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2294 4020 4199 Size: 416 53 83 399 24 877 1726 179 Cuts at: 4199 4404 4668 4934 4965 5571 5649 5667 6245 Size: 205 264 266 31 606 78 18 578 Cuts at: 6245 6687 7645 442 Size: 442 958 1753 Fragments arranged by size: 1753 1726 958 877 606 578 442 416 399 266 264 205 179 83 78 53 31 24 18 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4342 4342 Size: 8956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 3054 3104 3277 3501 3890 4079 4169 Size: 897 918 50 173 224 389 189 90 Cuts at: 4169 4182 4440 4667 4681 5778 6043 7616 1239 Size: 13 258 227 14 1097 265 1573 2579 Fragments arranged by size: 2579 1573 1097 918 897 389 265 258 227 224 189 173 90 50 14 13 Bpu10I CC'TnA_GC Cuts at: 2616 3785 4615 4822 4896 4920 5225 2616 Size: 1169 830 207 74 24 305 6347 Fragments arranged by size: 6347 1169 830 305 207 74 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5540 6057 7081 7946 8244 5540 Size: 517 1024 865 298 6252 Fragments arranged by size: 6252 1024 865 517 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3346 4704 5115 5713 5774 7619 882 Size: 617 1847 1358 411 598 61 1845 2219 Fragments arranged by size: 2219 1847 1845 1358 617 598 411 61 BsaAI yAC'GTr Cuts at: 493 1204 2428 2698 6253 6747 493 Size: 711 1224 270 3555 494 2702 Fragments arranged by size: 3555 2702 1224 711 494 270 BsaBI GATnn'nnATC Cuts at: 8950 8950 Size: 8956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3946 5016 6204 6763 Size: 53 83 186 936 2413 1070 1188 559 Cuts at: 6763 7145 7780 275 Size: 382 635 1451 Fragments arranged by size: 2413 1451 1188 1070 936 635 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2139 3153 4883 6078 7389 7787 8061 8208 8619 Size: 1014 1730 1195 1311 398 274 147 411 Cuts at: 8619 2139 Size: 2476 Fragments arranged by size: 2476 1730 1311 1195 1014 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4389 4419 4640 4670 4942 Size: 30 165 30 2986 30 221 30 272 Cuts at: 4942 4972 5708 5738 5867 5897 6146 6176 1178 Size: 30 736 30 129 30 249 30 3958 Fragments arranged by size: 3958 2986 736 272 249 221 165 129 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2083 3086 3089 3280 3889 4118 4413 4589 Size: 897 1003 3 191 609 229 295 176 Cuts at: 4589 1186 Size: 5553 Fragments arranged by size: 5553 1003 897 609 295 229 191 176 3 BseYI C'CCAG_C Cuts at: 2406 2859 3063 3378 3692 4093 4420 4478 4961 Size: 453 204 315 314 401 327 58 483 Cuts at: 4961 5583 5712 8159 8655 2406 Size: 622 129 2447 496 2707 Fragments arranged by size: 2707 2447 622 496 483 453 401 327 315 314 204 129 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2181 2221 2918 3172 3500 4184 4742 Size: 573 445 40 697 254 328 684 558 Cuts at: 4742 1163 Size: 5377 Fragments arranged by size: 5377 697 684 573 558 445 328 254 40 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3614 3959 6406 7167 8195 1540 Size: 275 186 1613 345 2447 761 1028 2301 Fragments arranged by size: 2447 2301 1613 1028 761 345 275 186 BsiHKAI G_wGCw'C Cuts at: 2319 2651 2946 5177 5816 5859 7017 7102 8173 Size: 332 295 2231 639 43 1158 85 1071 Cuts at: 8173 2319 Size: 3102 Fragments arranged by size: 3102 2231 1158 1071 639 332 295 85 43 BsmI GAATG_Cn' Cuts at: 5754 6467 6560 5754 Size: 713 93 8150 Fragments arranged by size: 8150 713 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3695 3695 Size: 8956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2319 2651 2946 3149 3825 4221 Size: 399 272 257 332 295 203 676 396 Cuts at: 4221 4317 4343 4492 4608 4673 4683 5033 5040 Size: 96 26 149 116 65 10 350 7 Cuts at: 5040 5177 5294 5584 5646 5682 5802 5816 5859 Size: 137 117 290 62 36 120 14 43 Cuts at: 5859 5897 6275 7017 7102 8173 1391 Size: 38 378 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 676 399 396 378 350 332 295 290 272 257 203 149 137 120 117 116 96 85 65 62 43 38 36 26 14 10 7 BspEI T'CCGG_A Cuts at: 2139 8619 2139 Size: 6480 2476 BspHI T'CATG_A Cuts at: 6845 8575 6845 Size: 1730 7226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2502 2550 3194 4341 5194 5265 6311 844 Size: 1658 48 644 1147 853 71 1046 3489 Fragments arranged by size: 3489 1658 1147 1046 853 644 71 48 BsrBI CCG'CTC Cuts at: 1394 1823 2392 2401 6843 1394 Size: 429 569 9 4442 3507 Fragments arranged by size: 4442 3507 569 429 9 BsrDI GCAATG_nn' Cuts at: 65 1248 3045 4595 5413 5472 7451 7625 65 Size: 1183 1797 1550 818 59 1979 174 1396 Fragments arranged by size: 1979 1797 1550 1396 1183 818 174 59 BsrFI r'CCGG_y Cuts at: 1700 1810 3589 3609 3852 4651 7600 7787 1700 Size: 110 1779 20 243 799 2949 187 2869 Fragments arranged by size: 2949 2869 1779 799 243 187 110 20 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8956 BssSI C'ACGA_G Cuts at: 3161 3410 4316 7016 8028 3161 Size: 249 906 2700 1012 4089 Fragments arranged by size: 4089 2700 1012 906 249 BstAPI GCAn_nnn'nTGC Cuts at: 794 2208 2941 4207 4225 794 Size: 1414 733 1266 18 5525 Fragments arranged by size: 5525 1414 1266 733 18 BstBI TT'CG_AA Cuts at: 2007 7760 2007 Size: 5753 3203 BstEII G'GTnAC_C Cuts at: 1184 2173 1184 Size: 989 7967 BstXI CCAn_nnnn'nTGG Cuts at: 3779 5012 5286 3779 Size: 1233 274 7449 Fragments arranged by size: 7449 1233 274 BstYI r'GATC_y Cuts at: 1972 2324 2772 3905 4563 5767 6114 6286 6653 Size: 352 448 1133 658 1204 347 172 367 Cuts at: 6653 7038 7055 8507 8593 8605 8951 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1204 1133 658 448 385 367 352 347 346 172 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4983 4983 Size: 8956 BtgI C'CryG_G Cuts at: 513 1065 2274 2664 3196 3273 3323 4499 5604 Size: 552 1209 390 532 77 50 1176 1105 Cuts at: 5604 513 Size: 3865 Fragments arranged by size: 3865 1209 1176 1105 552 532 390 77 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3123 5292 487 Size: 683 56 1897 2169 4151 Fragments arranged by size: 4151 2169 1897 683 56 BtsI GCAGTG_nn' Cuts at: 3793 4688 4726 6472 7266 7286 3793 Size: 895 38 1746 794 20 5463 Fragments arranged by size: 5463 1746 895 794 38 20 ClaI AT'CG_AT Cuts at: 1554 2181 1554 Size: 627 8329 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8921 DraI TTT'AAA Cuts at: 6260 6260 Size: 8956 DraIII CAC_nnn'GTG Cuts at: 2941 4207 4338 5579 2941 Size: 1266 131 1241 6318 Fragments arranged by size: 6318 1266 1241 131 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3338 7963 809 Size: 507 2022 4625 1802 Fragments arranged by size: 4625 2022 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2445 2799 2838 Size: 54 1425 50 275 18 615 354 39 Cuts at: 2838 3139 3607 3611 3672 3705 3854 4429 5151 Size: 301 468 4 61 33 149 575 722 Cuts at: 5151 5562 6129 6403 7292 8921 8 Size: 411 567 274 889 1629 43 Fragments arranged by size: 1629 1425 889 722 615 575 567 468 411 354 301 275 274 149 61 54 50 43 39 33 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3611 6403 1537 Size: 275 1799 2792 4090 Fragments arranged by size: 4090 2792 1799 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2552 2933 3717 3935 5772 6023 6886 1353 Size: 1199 381 784 218 1837 251 863 3423 Fragments arranged by size: 3423 1837 1199 863 784 381 251 218 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2801 3756 5048 5609 5689 7543 Size: 1064 138 1212 955 1292 561 80 1854 Cuts at: 7543 7917 8063 387 Size: 374 146 1280 Fragments arranged by size: 1854 1292 1280 1212 1064 955 561 374 146 138 80 EcoICRI GAG'CTC Cuts at: 5175 5175 Size: 8956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2235 2362 2866 3054 3104 3277 Size: 393 504 99 127 504 188 50 173 Cuts at: 3277 3501 3890 4079 4169 4182 4307 4440 4667 Size: 224 389 189 90 13 125 133 227 Cuts at: 4667 4681 5504 5778 6043 7019 7616 8403 8671 Size: 14 823 274 265 976 597 787 268 Cuts at: 8671 1239 Size: 1524 Fragments arranged by size: 1524 976 823 787 597 504 504 393 389 274 268 265 227 224 189 188 173 133 127 125 99 90 50 14 13 EcoNI CCTnn'n_nnAGG Cuts at: 2976 5483 5834 2976 Size: 2507 351 6098 Fragments arranged by size: 6098 2507 351 EcoO109I rG'GnC_Cy Cuts at: 3053 3449 3746 4063 4622 4669 4670 4812 4891 Size: 396 297 317 559 47 1 142 79 Cuts at: 4891 5078 5087 5282 5290 5494 5502 5599 5894 Size: 187 9 195 8 204 8 97 295 Cuts at: 5894 3053 Size: 6115 Fragments arranged by size: 6115 559 396 317 297 295 204 195 187 142 97 79 47 9 8 8 1 EcoRI G'AATT_C Cuts at: 6265 6643 6265 Size: 378 8578 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 6037 6037 Size: 8956 FseI GG_CCGG'CC Cuts at: 3613 3613 Size: 8956 FspI TGC'GCA Cuts at: 5753 7462 5753 Size: 1709 7247 HaeII r_GCGC'y Cuts at: 1678 2407 3949 4118 4829 5019 6207 8103 1678 Size: 729 1542 169 711 190 1188 1896 2531 Fragments arranged by size: 2531 1896 1542 1188 729 711 190 169 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3192 3224 4640 4672 4796 Size: 32 135 32 1620 32 1416 32 124 Cuts at: 4796 4828 4860 4889 4921 4942 4974 5288 5320 Size: 32 32 29 32 21 32 314 32 Cuts at: 5320 5867 5899 7597 7629 7671 7703 1373 Size: 547 32 1698 32 42 32 2626 Fragments arranged by size: 2626 1698 1620 1416 547 314 135 124 42 32 32 32 32 32 32 32 32 32 32 32 32 29 21 HincII GTy'rAC Cuts at: 669 1657 1938 3349 6300 6546 669 Size: 988 281 1411 2951 246 3079 Fragments arranged by size: 3079 2951 1411 988 281 246 HindIII A'AGCT_T Cuts at: 748 2732 748 Size: 1984 6972 HpaI GTT'AAC Cuts at: 6546 6546 Size: 8956 KasI G'GCGC_C Cuts at: 3945 5015 6203 3945 Size: 1070 1188 6698 Fragments arranged by size: 6698 1188 1070 KpnI G_GTAC'C Cuts at: 4372 4594 6281 4372 Size: 222 1687 7047 Fragments arranged by size: 7047 1687 222 MfeI C'AATT_G Cuts at: 6555 6555 Size: 8956 MluI A'CGCG_T Cuts at: 8832 8832 Size: 8956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4973 8070 8254 1305 Size: 3668 3097 184 2007 Fragments arranged by size: 3668 3097 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2801 2840 3674 5153 5564 Size: 54 1425 343 969 39 834 1479 411 Cuts at: 5564 6131 10 Size: 567 2835 Fragments arranged by size: 2835 1479 1425 969 834 567 411 343 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2426 2909 2951 3135 4257 4446 5010 Size: 648 1260 483 42 184 1122 189 564 Cuts at: 5010 5123 5807 6914 7273 7432 518 Size: 113 684 1107 359 159 2042 Fragments arranged by size: 2042 1260 1122 1107 684 648 564 483 359 189 184 159 113 42 MspA1I CmG'CkG Cuts at: 1606 2034 2479 3484 3626 4225 4281 5614 5819 Size: 428 445 1005 142 599 56 1333 205 Cuts at: 5819 5957 6372 7049 8197 8442 1606 Size: 138 415 677 1148 245 2120 Fragments arranged by size: 2120 1333 1148 1005 677 599 445 428 415 245 205 142 138 56 NaeI GCC'GGC Cuts at: 3591 3611 3591 Size: 20 8936 NarI GG'CG_CC Cuts at: 3946 5016 6204 3946 Size: 1070 1188 6698 Fragments arranged by size: 6698 1188 1070 NcoI C'CATG_G Cuts at: 513 1065 2664 513 Size: 552 1599 6805 Fragments arranged by size: 6805 1599 552 NdeI CA'TA_TG Cuts at: 387 4426 387 Size: 4039 4917 NgoMIV G'CCGG_C Cuts at: 3589 3609 3589 Size: 20 8936 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8956 NotI GC'GGCC_GC Cuts at: 6403 6403 Size: 8956 NspI r_CATG'y Cuts at: 1132 1229 2627 2761 3126 6314 7859 1132 Size: 97 1398 134 365 3188 1545 2229 Fragments arranged by size: 3188 2229 1545 1398 365 134 97 PasI CC'CwG_GG Cuts at: 1348 2062 3913 4067 5286 5498 5983 6150 1348 Size: 714 1851 154 1219 212 485 167 4154 Fragments arranged by size: 4154 1851 1219 714 485 212 167 154 PciI A'CATG_T Cuts at: 3122 7855 3122 Size: 4733 4223 PflMI CCAn_nnn'nTGG Cuts at: 1108 2797 4010 4427 4505 4601 4977 5439 5905 Size: 1689 1213 417 78 96 376 462 466 Cuts at: 5905 1108 Size: 4159 Fragments arranged by size: 4159 1689 1213 466 462 417 376 96 78 PfoI T'CCnGG_A Cuts at: 2124 4056 4456 4658 5421 2124 Size: 1932 400 202 763 5659 Fragments arranged by size: 5659 1932 763 400 202 PmeI GTTT'AAAC Cuts at: 6260 6260 Size: 8956 PmlI CAC'GTG Cuts at: 2698 2698 Size: 8956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3320 3352 4389 4421 7025 7057 8538 8570 3320 Size: 32 1037 32 2604 32 1481 32 3706 Fragments arranged by size: 3706 2604 1481 1037 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3449 4063 4622 4812 5282 5494 5599 3449 Size: 614 559 190 470 212 105 6806 Fragments arranged by size: 6806 614 559 470 212 190 105 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8956 PsiI TTA'TAA Cuts at: 6526 6526 Size: 8956 PspOMI G'GGCC_C Cuts at: 4669 5290 5642 5893 4669 Size: 621 352 251 7732 Fragments arranged by size: 7732 621 352 251 PspXI vC'TCGA_Gb Cuts at: 5082 5082 Size: 8956 PstI C_TGCA'G Cuts at: 830 2782 2853 4639 5852 5926 6308 830 Size: 1952 71 1786 1213 74 382 3478 Fragments arranged by size: 3478 1952 1786 1213 382 74 71 PvuI CG_AT'CG Cuts at: 2001 3959 2001 Size: 1958 6998 PvuII CAG'CTG Cuts at: 3626 4281 5614 5957 3626 Size: 655 1333 343 6625 Fragments arranged by size: 6625 1333 655 343 SacI G_AGCT'C Cuts at: 5177 5177 Size: 8956 SalI G'TCGA_C Cuts at: 1936 3347 6298 1936 Size: 1411 2951 4594 Fragments arranged by size: 4594 2951 1411 SanDI GG'GwC_CC Cuts at: 3449 5599 3449 Size: 2150 6806 SapI GCTCTTCn'nnn_ Cuts at: 2552 3717 6023 2552 Size: 1165 2306 5485 Fragments arranged by size: 5485 2306 1165 SbfI CC_TGCA'GG Cuts at: 6308 6308 Size: 8956 ScaI AGT'ACT Cuts at: 7204 8916 7204 Size: 1712 7244 SexAI A'CCwGG_T Cuts at: 4808 4808 Size: 8956 SfcI C'TryA_G Cuts at: 826 1045 2764 2778 2849 3621 4122 4276 4635 Size: 219 1719 14 71 772 501 154 359 Cuts at: 4635 5848 5922 5935 6304 7439 8120 8311 826 Size: 1213 74 13 369 1135 681 191 1471 Fragments arranged by size: 1719 1471 1213 1135 772 681 501 369 359 219 191 154 74 71 14 13 SfiI GGCCn_nnn'nGGCC Cuts at: 5085 5085 Size: 8956 SfoI GGC'GCC Cuts at: 3947 5017 6205 3947 Size: 1070 1188 6698 Fragments arranged by size: 6698 1188 1070 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8956 SmaI CCC'GGG Cuts at: 1872 3446 4521 5263 5912 6283 1872 Size: 1574 1075 742 649 371 4545 Fragments arranged by size: 4545 1574 1075 742 649 371 SmlI C'TyrA_G Cuts at: 820 1017 1943 5082 5555 6072 7060 7961 8223 Size: 197 926 3139 473 517 988 901 262 Cuts at: 8223 820 Size: 1553 Fragments arranged by size: 3139 1553 988 926 901 517 473 262 197 SnaBI TAC'GTA Cuts at: 493 6253 6747 493 Size: 5760 494 2702 Fragments arranged by size: 5760 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8956 SphI G_CATG'C Cuts at: 1132 2627 6314 1132 Size: 1495 3687 3774 Fragments arranged by size: 3774 3687 1495 SrfI GCCC'GGGC Cuts at: 5263 5912 5263 Size: 649 8307 SspI AAT'ATT Cuts at: 5 52 6880 5 Size: 47 6828 2081 Fragments arranged by size: 6828 2081 47 StuI AGG'CCT Cuts at: 1517 2098 2264 3239 3778 3929 4856 1517 Size: 581 166 975 539 151 927 5617 Fragments arranged by size: 5617 975 927 581 539 166 151 StyI C'CwwG_G Cuts at: 513 1065 2099 2592 2664 3219 3655 4296 5533 Size: 552 1034 493 72 555 436 641 1237 Cuts at: 5533 5703 6395 513 Size: 170 692 3074 Fragments arranged by size: 3074 1237 1034 692 641 555 552 493 436 170 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3328 4218 4309 4686 5590 6983 7000 Size: 1255 1748 890 91 377 904 1393 17 Cuts at: 7000 7153 7338 7783 325 Size: 153 185 445 1498 Fragments arranged by size: 1748 1498 1393 1255 904 890 445 377 185 153 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 7202 8914 96 Size: 274 80 33 51 1443 5225 1712 138 Fragments arranged by size: 5225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2309 2341 2630 2662 3433 3465 4753 4785 5032 Size: 32 289 32 771 32 1288 32 247 Cuts at: 5032 5064 5175 5207 5708 5740 6146 6178 8916 Size: 32 111 32 501 32 406 32 2738 Cuts at: 8916 8948 2309 Size: 32 2317 Fragments arranged by size: 2738 2317 1288 771 501 406 289 247 111 32 32 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4842 5881 1316 Size: 3526 1039 4391 Fragments arranged by size: 4391 3526 1039 XbaI T'CTAG_A Cuts at: 6292 6292 Size: 8956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 3375 5540 6232 2038 Size: 1337 2165 692 4762 Fragments arranged by size: 4762 2165 1337 692 XhoI C'TCGA_G Cuts at: 1943 5082 1943 Size: 3139 5817 XmaI C'CCGG_G Cuts at: 1870 3444 4519 5261 5910 6281 1870 Size: 1574 1075 742 649 371 4545 Fragments arranged by size: 4545 1574 1075 742 649 371 XmnI GAAnn'nnTTC Cuts at: 2135 6017 7085 2135 Size: 3882 1068 4006 Fragments arranged by size: 4006 3882 1068 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6764 276 Size: 53 83 186 936 5230 2468 Fragments arranged by size: 5230 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FspAI NruI NsiI PacI PsrI RsrII SacII SwaI