Restriction map for
pFN21ASDA1067
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbrPI BbvCI BciVI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspEI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 652 GTCTAC GT^[AC][GT]AC
AcsI 457 GAATTT [GA]^AATT[CT]
AcyI 1408 GGCGCC G[GA]^CG[CT]C
Alw44I 187 GTGCAC G^TGCAC
ApaI 309 GGGCCC GGGCC^C
ApaLI 187 GTGCAC G^TGCAC
ApoI 457 GAATTT [GA]^AATT[CT]
Asp700I 1023 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1408 GGCGCC GGCGC^C
BcgI 2040 CGACACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 518 TGATCA T^GATCA
BfaI 859 CTAG C^TAG
BsaHI 1408 GGCGCC G[GA]^CG[CT]C
BsgI 1576 CTGCAC GTGCAG(16/14)
BsiEI 2028 CGATCG CG[GA][CT]^CG
BspDI 2026 ATCGAT AT^CGAT
BspHI 644 TCATGA T^CATGA
BsrDI 1358 GCAATG GCAATG(2/0)
BssSI 1345 CACGAG CACGAG(-5/-1)
BstAPI 210 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2026 ATCGAT AT^CGAT
FbaI 518 TGATCA T^GATCA
Hin1I 1408 GGCGCC G[GA]^CG[CT]C
KasI 1408 GGCGCC G^GCGCC
MaeI 859 CTAG C^TAG
NarI 1408 GGCGCC GG^CGCC
NcoI 1247 CCATGG C^CATGG
PpuMI 1550 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1914 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2028 CGATCG CGAT^CG
RcaI 644 TCATGA T^CATGA
SacI 1348 GAGCTC GAGCT^C
SexAI 1187 ACCAGGT A^CC[AT]GGT
SfoI 1408 GGCGCC GGC^GCC
StuI 1859 AGGCCT AGG^CCT
XbaI 858 TCTAGA T^CTAGA
XcmI 314 CCACAGGTAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1023 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 433 CGCG CG^CG
476 CGCG
AlwNI 211 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
985 CAGCTGCTG
Aor51HI 1269 AGCGCT AGC^GCT
1661 AGCGCT
AvaI 574 CCCGAG C^[CT]CG[GA]G
1006 CCCGAG
BalI 278 TGGCCA TGG^CCA
347 TGGCCA
BglI 1096 GCCCGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1411 GCCATGCTGGC
BseRI 61 GAGGAG GAGGAG(10/8)
64 GAGGAG
BsmBI 492 CGTCTC CGTCTC(1/5)
1399 GAGACG
BsoBI 574 CCCGAG C^[CT]CG[GA]G
1006 CCCGAG
BsrI 281 CCAGT ACTGG(1/-1)
793 CCAGT
BstUI 433 CGCG CG^CG
476 CGCG
BstXI 671 CCAGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1265 CCAAAGCGCTGG
BstYI 513 GGATCT [GA]^GATC[CT]
1691 AGATCC
DsaI 1127 CCACGG C^C[GA][CT]GG
1247 CCATGG
Eco47III 1269 AGCGCT AGC^GCT
1661 AGCGCT
EcoNI 892 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1038 CCTGATACAGG
EcoT14I 1247 CCATGG C^C[AT][AT]GG
1538 CCAAGG
MflI 513 GGATCT [GA]^GATC[CT]
1691 AGATCC
MluNI 278 TGGCCA TGG^CCA
347 TGGCCA
MscI 278 TGGCCA TGG^CCA
347 TGGCCA
MseI 1717 TTAA T^TAA
1814 TTAA
MslI 180 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1242 CACATCCATG
MvnI 433 CGCG CG^CG
476 CGCG
NspI 911 ACATGC [GA]CATG^[CT]
1322 ACATGC
PflMI 984 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1629 CCAGCTGGTGG
SapI 99 GAAGAGC GCTCTTC(1/4)
1436 GCTCTTC
SmlI 1162 CTCAAG C^T[CT][GA]AG
1600 CTCAAG
StyI 1247 CCATGG C^C[AT][AT]GG
1538 CCAAGG
Tru9I 1717 TTAA T^TAA
1814 TTAA
Tsp509I 458 AATT ^AATT
1591 AATT
Van91I 984 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1629 CCAGCTGGTGG
XhoII 513 GGATCT [GA]^GATC[CT]
1691 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AspI AvaII BanI BanII BbsI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
TseI Tsp45I TspRI Tth111I TthHB8I