(Circular) (Six-base) MAPSORT of: pFN21ASDA1067.seq Check: 9213 from: 1 to: 6781 pFN21ASDA1067 6781 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2601 4024 2601 Size: 1423 5358 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4591 278 Size: 53 83 186 936 3055 2468 Fragments arranged by size: 3055 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2676 4124 1937 Size: 739 1448 4594 Fragments arranged by size: 4594 1448 739 Acc65I G'GTAC_C Cuts at: 4102 4102 Size: 6781 AclI AA'CG_TT Cuts at: 1563 4908 5281 1563 Size: 3345 373 3063 Fragments arranged by size: 3345 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2744 4844 6228 6496 1632 Size: 464 648 2100 1384 268 1917 Fragments arranged by size: 2100 1917 1384 648 464 268 AfeI AGC'GCT Cuts at: 3294 3686 3294 Size: 392 6389 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6584 AflIII A'CryG_T Cuts at: 1203 5680 6657 1203 Size: 4477 977 1327 Fragments arranged by size: 4477 1327 977 AgeI A'CCGG_T Cuts at: 5612 5612 Size: 6781 AhdI GACnn_n'nnGTC Cuts at: 5510 5510 Size: 6781 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6781 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3515 1184 Size: 2331 4450 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2011 2043 2011 Size: 32 6749 AlwNI CAG_nnn'CTG Cuts at: 2239 3013 4524 6096 2239 Size: 774 1511 1572 2924 Fragments arranged by size: 2924 1572 1511 774 ApaI G_GGCC'C Cuts at: 2336 2336 Size: 6781 ApaLI G'TGCA_C Cuts at: 2210 4838 5994 2210 Size: 2628 1156 2997 Fragments arranged by size: 2997 2628 1156 ApoI r'AATT_y Cuts at: 1493 2480 4090 4316 4468 6577 1493 Size: 987 1610 226 152 2109 1697 Fragments arranged by size: 2109 1697 1610 987 226 152 AseI AT'TA_AT Cuts at: 160 1032 5335 160 Size: 872 4303 1606 Fragments arranged by size: 4303 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 AvaI C'yCGr_G Cuts at: 1870 1943 2597 3029 4106 1870 Size: 73 654 432 1077 4545 Fragments arranged by size: 4545 1077 654 432 73 BamHI G'GATC_C Cuts at: 4111 4478 4111 Size: 367 6414 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3284 3359 3431 4102 5557 Size: 325 217 627 1497 75 72 671 1455 Cuts at: 5557 618 Size: 1842 Fragments arranged by size: 1842 1497 1455 671 627 325 217 75 72 BanII G_rGCy'C Cuts at: 1391 2336 2903 3375 4100 1391 Size: 945 567 472 725 4072 Fragments arranged by size: 4072 945 725 567 472 BbeI G_GCGC'C Cuts at: 3435 3435 Size: 6781 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2264 3053 3157 3680 3941 928 Size: 971 365 789 104 523 261 3768 Fragments arranged by size: 3768 971 789 523 365 261 104 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4052 4086 4972 5006 1903 Size: 34 2115 34 886 34 3678 Fragments arranged by size: 3678 2115 886 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4673 5883 4673 Size: 1210 5571 BclI T'GATC_A Cuts at: 1548 2541 1548 Size: 993 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3125 3440 5392 Size: 107 122 71 631 605 1453 315 1952 Cuts at: 5392 136 Size: 1525 Fragments arranged by size: 1952 1525 1453 631 605 315 122 107 71 BglII A'GATC_T Cuts at: 6776 6776 Size: 6781 BlpI GC'TnA_GC Cuts at: 3503 3551 3681 4198 3503 Size: 48 130 517 6086 Fragments arranged by size: 6086 517 130 48 Bme1580I G_kGCm'C Cuts at: 1790 2214 2336 3227 3990 4842 5998 1790 Size: 424 122 891 763 852 1156 2573 Fragments arranged by size: 2573 1156 891 852 763 424 122 BmgBI CAC'GTC Cuts at: 1318 2578 2944 1318 Size: 1260 366 5155 Fragments arranged by size: 5155 1260 366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4512 5470 442 Size: 416 53 83 399 24 3095 958 1753 Fragments arranged by size: 3095 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6781 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3475 3815 3475 Size: 340 6441 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2210 2492 2582 2594 2606 2677 3203 Size: 899 72 282 90 12 12 71 526 Cuts at: 3203 3317 3653 5441 1239 Size: 114 336 1788 2579 Fragments arranged by size: 2579 1788 899 526 336 282 114 90 72 71 12 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3170 3608 4906 5771 6069 3170 Size: 438 1298 865 298 3882 Fragments arranged by size: 3882 1298 865 438 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2942 3269 3446 3944 5444 882 Size: 617 1443 327 177 498 1500 2219 Fragments arranged by size: 2219 1500 1443 617 498 327 177 BsaAI yAC'GTr Cuts at: 493 1204 4078 4572 493 Size: 711 2874 494 2702 Fragments arranged by size: 2874 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6775 6775 Size: 6781 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3432 4588 4970 5605 Size: 53 83 186 936 1899 1156 382 635 Cuts at: 5605 275 Size: 1451 Fragments arranged by size: 1899 1451 1156 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5214 5612 5886 6033 6444 5214 Size: 398 274 147 411 5551 Fragments arranged by size: 5551 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2081 2111 3131 3161 1178 Size: 30 165 30 678 30 1020 30 4798 Fragments arranged by size: 4798 1020 678 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2102 1186 Size: 913 3 5865 Fragments arranged by size: 5865 913 3 BseYI C'CCAG_C Cuts at: 2871 3526 3577 5984 6480 2871 Size: 655 51 2407 496 3172 Fragments arranged by size: 3172 2407 655 496 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3584 1163 Size: 573 1848 4360 Fragments arranged by size: 4360 1848 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4054 4231 4992 6020 1540 Size: 275 186 2053 177 761 1028 2301 Fragments arranged by size: 2301 2053 1028 761 275 186 177 BsiHKAI G_wGCw'C Cuts at: 2214 2529 2592 2610 3036 3159 3219 3375 3702 Size: 315 63 18 426 123 60 156 327 Cuts at: 3702 4842 4927 5998 2214 Size: 1140 85 1071 2997 Fragments arranged by size: 2997 1140 1071 426 327 315 156 123 85 63 60 18 BsmI GAATG_Cn' Cuts at: 4292 4385 4292 Size: 93 6688 BsmBI CGTCTCn'nnnn_ Cuts at: 2521 3416 2521 Size: 895 5886 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2336 2529 2592 2610 2903 3036 Size: 399 424 122 193 63 18 293 133 Cuts at: 3036 3159 3219 3227 3375 3702 3990 4100 4842 Size: 123 60 8 148 327 288 110 742 Cuts at: 4842 4927 5998 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 399 327 293 288 193 148 133 123 122 110 85 63 60 18 8 BspEI T'CCGG_A Cuts at: 6444 6444 Size: 6781 BspHI T'CATG_A Cuts at: 2667 4670 6400 2667 Size: 2003 1730 3048 Fragments arranged by size: 3048 2003 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2601 3543 4024 4136 844 Size: 1757 942 481 112 3489 Fragments arranged by size: 3489 1757 942 481 112 BsrBI CCG'CTC Cuts at: 1394 1823 2048 2709 3814 4668 1394 Size: 429 225 661 1105 854 3507 Fragments arranged by size: 3507 1105 854 661 429 225 BsrDI GCAATG_nn' Cuts at: 65 1248 3388 5276 5450 65 Size: 1183 2140 1888 174 1396 Fragments arranged by size: 2140 1888 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5425 5612 1700 Size: 110 3615 187 2869 Fragments arranged by size: 3615 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6781 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6781 BssSI C'ACGA_G Cuts at: 3368 4841 5853 3368 Size: 1473 1012 4296 Fragments arranged by size: 4296 1473 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 794 Size: 1445 5336 BstBI TT'CG_AA Cuts at: 2007 5585 2007 Size: 3578 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6781 BstXI CCAn_nnnn'nTGG Cuts at: 2701 3295 2701 Size: 594 6187 BstYI r'GATC_y Cuts at: 1972 2536 3714 4111 4478 4863 4880 6332 6418 Size: 564 1178 397 367 385 17 1452 86 Cuts at: 6418 6430 6776 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1178 564 397 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3150 3270 513 Size: 552 2085 120 4024 Fragments arranged by size: 4024 2085 552 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3742 487 Size: 683 56 2516 3526 Fragments arranged by size: 3526 2516 683 56 BtsI GCAGTG_nn' Cuts at: 2394 2965 3669 4297 5091 5111 2394 Size: 571 704 628 794 20 4064 Fragments arranged by size: 4064 794 704 628 571 20 ClaI AT'CG_AT Cuts at: 1554 4050 1554 Size: 2496 4285 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6746 DraI TTT'AAA Cuts at: 4085 4085 Size: 6781 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5788 809 Size: 507 4472 1802 Fragments arranged by size: 4472 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2301 2370 2650 Size: 54 1425 50 275 18 471 69 280 Cuts at: 2650 2825 3015 3465 4228 5117 6746 8 Size: 175 190 450 763 889 1629 43 Fragments arranged by size: 1629 1425 889 763 471 450 280 275 190 175 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4228 1537 Size: 275 2416 4090 Fragments arranged by size: 4090 2416 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 2346 3398 3466 4711 1353 Size: 764 229 1052 68 1245 3423 Fragments arranged by size: 3423 1245 1052 764 229 68 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5368 5742 5888 387 Size: 1064 138 3779 374 146 1280 Fragments arranged by size: 3779 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3373 3373 Size: 6781 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2138 2210 2492 2582 2594 2606 Size: 393 464 42 72 282 90 12 12 Cuts at: 2606 2677 2744 3203 3317 3653 4844 5441 6228 Size: 71 67 459 114 336 1191 597 787 Cuts at: 6228 6496 1239 Size: 268 1524 Fragments arranged by size: 1524 1191 787 597 464 459 393 336 282 268 114 90 72 71 67 42 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 2919 3065 2919 Size: 146 6635 EcoO109I rG'GnC_Cy Cuts at: 2332 2333 2467 3574 3960 2332 Size: 1 134 1107 386 5153 Fragments arranged by size: 5153 1107 386 134 1 EcoRI G'AATT_C Cuts at: 4090 4468 4090 Size: 378 6403 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3067 3067 Size: 6781 FspI TGC'GCA Cuts at: 5287 5287 Size: 6781 HaeII r_GCGC'y Cuts at: 1678 3296 3435 3591 3688 5928 1678 Size: 1618 139 156 97 2240 2531 Fragments arranged by size: 2531 2240 1618 156 139 97 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2212 2244 5422 Size: 32 135 32 509 32 99 32 3178 Cuts at: 5422 5454 5496 5528 1373 Size: 32 42 32 2626 Fragments arranged by size: 3178 2626 509 135 99 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4125 4371 669 Size: 988 281 2187 246 3079 Fragments arranged by size: 3079 2187 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6781 HpaI GTT'AAC Cuts at: 4371 4371 Size: 6781 KasI G'GCGC_C Cuts at: 3431 3431 Size: 6781 KpnI G_GTAC'C Cuts at: 4106 4106 Size: 6781 MfeI C'AATT_G Cuts at: 4380 4380 Size: 6781 MluI A'CGCG_T Cuts at: 6657 6657 Size: 6781 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5895 6079 1305 Size: 4590 184 2007 Fragments arranged by size: 4590 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 2372 10 Size: 54 1425 343 471 69 4419 Fragments arranged by size: 4419 1425 471 343 69 54 MslI CAynn'nnrTG Cuts at: 518 1166 2207 3269 4739 5098 5257 518 Size: 648 1041 1062 1470 359 159 2042 Fragments arranged by size: 2042 1470 1062 1041 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2236 2634 2701 3010 3655 3735 4197 4874 Size: 630 398 67 309 645 80 462 677 Cuts at: 4874 6022 6267 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 645 630 462 398 309 245 80 67 NarI GG'CG_CC Cuts at: 3432 3432 Size: 6781 NcoI C'CATG_G Cuts at: 513 1065 3270 513 Size: 552 2205 4024 Fragments arranged by size: 4024 2205 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6781 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6781 NotI GC'GGCC_GC Cuts at: 4228 4228 Size: 6781 NspI r_CATG'y Cuts at: 1132 1229 2938 3349 4139 5684 1132 Size: 97 1709 411 790 1545 2229 Fragments arranged by size: 2229 1709 1545 790 411 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6781 PciI A'CATG_T Cuts at: 5680 5680 Size: 6781 PflMI CCAn_nnn'nTGG Cuts at: 1108 3013 3658 1108 Size: 1905 645 4231 Fragments arranged by size: 4231 1905 645 PfoI T'CCnGG_A Cuts at: 2400 2610 3417 3801 2400 Size: 210 807 384 5380 Fragments arranged by size: 5380 807 384 210 PmeI GTTT'AAAC Cuts at: 4085 4085 Size: 6781 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4850 4882 6363 6395 4850 Size: 32 1481 32 5236 Fragments arranged by size: 5236 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3574 3574 Size: 6781 PshAI GACnn'nnGTC Cuts at: 1613 3941 1613 Size: 2328 4453 PsiI TTA'TAA Cuts at: 4351 4351 Size: 6781 PspOMI G'GGCC_C Cuts at: 2332 2332 Size: 6781 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2663 2695 2663 Size: 32 6749 PstI C_TGCA'G Cuts at: 830 2235 2244 3402 3540 4133 830 Size: 1405 9 1158 138 593 3478 Fragments arranged by size: 3478 1405 1158 593 138 9 PvuI CG_AT'CG Cuts at: 2001 4054 2001 Size: 2053 4728 PvuII CAG'CTG Cuts at: 2701 3010 3655 3735 2701 Size: 309 645 80 5747 Fragments arranged by size: 5747 645 309 80 SacI G_AGCT'C Cuts at: 3375 3375 Size: 6781 SalI G'TCGA_C Cuts at: 1936 4123 1936 Size: 2187 4594 SapI GCTCTTCn'nnn_ Cuts at: 2117 3466 2117 Size: 1349 5432 SbfI CC_TGCA'GG Cuts at: 4133 4133 Size: 6781 ScaI AGT'ACT Cuts at: 5029 6741 5029 Size: 1712 5069 SexAI A'CCwGG_T Cuts at: 3210 3210 Size: 6781 SfcI C'TryA_G Cuts at: 826 1045 2231 2240 3398 3475 3536 3968 4129 Size: 219 1186 9 1158 77 61 432 161 Cuts at: 4129 5264 5945 6136 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1186 1158 1135 681 432 219 191 161 77 61 9 SfoI GGC'GCC Cuts at: 3433 3433 Size: 6781 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6781 SmaI CCC'GGG Cuts at: 1872 4108 1872 Size: 2236 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3185 3623 4885 5786 6048 820 Size: 197 926 1242 438 1262 901 262 1553 Fragments arranged by size: 1553 1262 1242 926 901 438 262 197 SnaBI TAC'GTA Cuts at: 493 4078 4572 493 Size: 3585 494 2702 Fragments arranged by size: 3585 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6781 SphI G_CATG'C Cuts at: 1132 4139 1132 Size: 3007 3774 SspI AAT'ATT Cuts at: 5 52 4705 5 Size: 47 4653 2081 Fragments arranged by size: 4653 2081 47 StuI AGG'CCT Cuts at: 1517 3884 1517 Size: 2367 4414 StyI C'CwwG_G Cuts at: 513 1065 3270 3561 4220 513 Size: 552 2205 291 659 3074 Fragments arranged by size: 3074 2205 659 552 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2966 4808 4825 4978 5163 5608 325 Size: 1255 1386 1842 17 153 185 445 1498 Fragments arranged by size: 1842 1498 1386 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3339 4011 5027 Size: 274 80 33 51 1443 1362 672 1016 Cuts at: 5027 6739 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1362 1016 672 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3131 3163 6741 6773 3131 Size: 32 3578 32 3139 Fragments arranged by size: 3578 3139 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2095 2263 2279 1316 Size: 779 168 16 5818 Fragments arranged by size: 5818 779 168 16 XbaI T'CTAG_A Cuts at: 2881 4117 2881 Size: 1236 5545 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2344 2344 Size: 6781 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6781 XmaI C'CCGG_G Cuts at: 1870 4106 1870 Size: 2236 4545 XmnI GAAnn'nnTTC Cuts at: 3050 4910 3050 Size: 1860 4921 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4589 276 Size: 53 83 186 936 3055 2468 Fragments arranged by size: 3055 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SanDI SfiI SrfI SwaI