Restriction map for
pFN21ASDA1067
(Linear) (Six-base) MAPSORT of: KIAA1067.seq Check: 7327 from: 1 to: 2052
KIAA1067.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 578 2001 2052
Size: 578 1423 51
Fragments arranged by size:
1423 578 51
AccI GT'mk_AC
Cuts at: 0 653 2052
Size: 653 1399
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 721 2052
Size: 73 648 1331
Fragments arranged by size:
1331 648 73
AfeI AGC'GCT
Cuts at: 0 1271 1663 2052
Size: 1271 392 389
Fragments arranged by size:
1271 392 389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1492 2052
Size: 1492 560
AlwNI CAG_nnn'CTG
Cuts at: 0 216 990 2052
Size: 216 774 1062
Fragments arranged by size:
1062 774 216
ApaI G_GGCC'C
Cuts at: 0 313 2052
Size: 313 1739
ApaLI G'TGCA_C
Cuts at: 0 187 2052
Size: 187 1865
ApoI r'AATT_y
Cuts at: 0 457 2052
Size: 457 1595
AvaI C'yCGr_G
Cuts at: 0 574 1006 2052
Size: 574 432 1046
Fragments arranged by size:
1046 574 432
BanI G'GyrC_C
Cuts at: 0 1261 1336 1408 2052
Size: 1261 75 72 644
Fragments arranged by size:
1261 644 75 72
BanII G_rGCy'C
Cuts at: 0 313 880 1352 2052
Size: 313 567 472 700
Fragments arranged by size:
700 567 472 313
BbeI G_GCGC'C
Cuts at: 0 1412 2052
Size: 1412 640
BbsI GAAGACnn'nnnn_
Cuts at: 0 241 1030 1134 1657 1918 2052
Size: 241 789 104 523 261 134
Fragments arranged by size:
789 523 261 241 134 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2029 2052
Size: 2029 23
BclI T'GATC_A
Cuts at: 0 518 2052
Size: 518 1534
BglI GCCn_nnn'nGGC
Cuts at: 0 1102 1417 2052
Size: 1102 315 635
Fragments arranged by size:
1102 635 315
BlpI GC'TnA_GC
Cuts at: 0 1480 1528 1658 2052
Size: 1480 48 130 394
Fragments arranged by size:
1480 394 130 48
Bme1580I G_kGCm'C
Cuts at: 0 191 313 1204 1967 2052
Size: 191 122 891 763 85
Fragments arranged by size:
891 763 191 122 85
BmgBI CAC'GTC
Cuts at: 0 555 921 2052
Size: 555 366 1131
Fragments arranged by size:
1131 555 366
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1452 1792 2052
Size: 1452 340 260
Fragments arranged by size:
1452 340 260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 187 469 559 571 583 654 1180
Size: 115 72 282 90 12 12 71 526
Cuts at: 1180 1294 1630 2052
Size: 114 336 422
Fragments arranged by size:
526 422 336 282 115 114 90 72
71 12 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 1585 2052
Size: 1147 438 467
Fragments arranged by size:
1147 467 438
BsaI GGTCTCn'nnnn_
Cuts at: 0 919 1246 1423 1921 2052
Size: 919 327 177 498 131
Fragments arranged by size:
919 498 327 177 131
BsaHI Gr'CG_yC
Cuts at: 0 1409 2052
Size: 1409 643
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 58 88 1108 1138 2052
Size: 58 30 1020 30 914
Fragments arranged by size:
1020 914 58 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 79 2052
Size: 76 3 1973
Fragments arranged by size:
1973 76 3
BseYI C'CCAG_C
Cuts at: 0 848 1503 1554 2052
Size: 848 655 51 498
Fragments arranged by size:
848 655 498 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1561 2052
Size: 1561 491
BsiEI CG_ry'CG
Cuts at: 0 2031 2052
Size: 2031 21
BsiHKAI G_wGCw'C
Cuts at: 0 191 506 569 587 1013 1136 1196 1352
Size: 191 315 63 18 426 123 60 156
Cuts at: 1352 1679 2052
Size: 327 373
Fragments arranged by size:
426 373 327 315 191 156 123 63
60 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 498 1393 2052
Size: 498 895 659
Fragments arranged by size:
895 659 498
Bsp1286I G_dGCh'C
Cuts at: 0 191 313 506 569 587 880 1013 1136
Size: 191 122 193 63 18 293 133 123
Cuts at: 1136 1196 1204 1352 1679 1967 2052
Size: 60 8 148 327 288 85
Fragments arranged by size:
327 293 288 193 191 148 133 123
122 85 63 60 18 8
BspHI T'CATG_A
Cuts at: 0 644 2052
Size: 644 1408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 578 1520 2001 2052
Size: 578 942 481 51
Fragments arranged by size:
942 578 481 51
BsrBI CCG'CTC
Cuts at: 0 25 686 1791 2052
Size: 25 661 1105 261
Fragments arranged by size:
1105 661 261 25
BsrDI GCAATG_nn'
Cuts at: 0 1365 2052
Size: 1365 687
BssSI C'ACGA_G
Cuts at: 0 1345 2052
Size: 1345 707
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 2052
Size: 216 1836
BstXI CCAn_nnnn'nTGG
Cuts at: 0 678 1272 2052
Size: 678 594 780
Fragments arranged by size:
780 678 594
BstYI r'GATC_y
Cuts at: 0 513 1691 2052
Size: 513 1178 361
Fragments arranged by size:
1178 513 361
BtgI C'CryG_G
Cuts at: 0 1127 1247 2052
Size: 1127 120 805
Fragments arranged by size:
1127 805 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1719 2052
Size: 1719 333
BtsI GCAGTG_nn'
Cuts at: 0 371 942 1646 2052
Size: 371 571 704 406
Fragments arranged by size:
704 571 406 371
ClaI AT'CG_AT
Cuts at: 0 2027 2052
Size: 2027 25
EaeI y'GGCC_r
Cuts at: 0 278 347 627 802 992 1442 2052
Size: 278 69 280 175 190 450 610
Fragments arranged by size:
610 450 280 278 190 175 69
EarI CTCTTCn'nnn_
Cuts at: 0 94 323 1375 1443 2052
Size: 94 229 1052 68 609
Fragments arranged by size:
1052 609 229 94 68
EcoICRI GAG'CTC
Cuts at: 0 1350 2052
Size: 1350 702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 115 187 469 559 571 583 654
Size: 73 42 72 282 90 12 12 71
Cuts at: 654 721 1180 1294 1630 2052
Size: 67 459 114 336 422
Fragments arranged by size:
459 422 336 282 114 90 73 72
71 67 42 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 896 1042 2052
Size: 896 146 1010
Fragments arranged by size:
1010 896 146
EcoO109I rG'GnC_Cy
Cuts at: 0 309 310 444 1551 1937 2052
Size: 309 1 134 1107 386 115
Fragments arranged by size:
1107 386 309 134 115 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1044 2052
Size: 1044 1008
HaeII r_GCGC'y
Cuts at: 0 1273 1412 1568 1665 2052
Size: 1273 139 156 97 387
Fragments arranged by size:
1273 387 156 139 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 189 221 2052
Size: 58 32 99 32 1831
Fragments arranged by size:
1831 99 58 32 32
KasI G'GCGC_C
Cuts at: 0 1408 2052
Size: 1408 644
MscI TGG'CCA
Cuts at: 0 280 349 2052
Size: 280 69 1703
Fragments arranged by size:
1703 280 69
MslI CAynn'nnrTG
Cuts at: 0 184 1246 2052
Size: 184 1062 806
Fragments arranged by size:
1062 806 184
MspA1I CmG'CkG
Cuts at: 0 213 611 678 987 1632 1712 2052
Size: 213 398 67 309 645 80 340
Fragments arranged by size:
645 398 340 309 213 80 67
NarI GG'CG_CC
Cuts at: 0 1409 2052
Size: 1409 643
NcoI C'CATG_G
Cuts at: 0 1247 2052
Size: 1247 805
NspI r_CATG'y
Cuts at: 0 915 1326 2052
Size: 915 411 726
Fragments arranged by size:
915 726 411
PflMI CCAn_nnn'nTGG
Cuts at: 0 990 1635 2052
Size: 990 645 417
Fragments arranged by size:
990 645 417
PfoI T'CCnGG_A
Cuts at: 0 377 587 1394 1778 2052
Size: 377 210 807 384 274
Fragments arranged by size:
807 384 377 274 210
PpuMI rG'GwC_Cy
Cuts at: 0 1551 2052
Size: 1551 501
PshAI GACnn'nnGTC
Cuts at: 0 1918 2052
Size: 1918 134
PspOMI G'GGCC_C
Cuts at: 0 309 2052
Size: 309 1743
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 640 672 2052
Size: 640 32 1380
Fragments arranged by size:
1380 640 32
PstI C_TGCA'G
Cuts at: 0 212 221 1379 1517 2052
Size: 212 9 1158 138 535
Fragments arranged by size:
1158 535 212 138 9
PvuI CG_AT'CG
Cuts at: 0 2031 2052
Size: 2031 21
PvuII CAG'CTG
Cuts at: 0 678 987 1632 1712 2052
Size: 678 309 645 80 340
Fragments arranged by size:
678 645 340 309 80
SacI G_AGCT'C
Cuts at: 0 1352 2052
Size: 1352 700
SapI GCTCTTCn'nnn_
Cuts at: 0 94 1443 2052
Size: 94 1349 609
Fragments arranged by size:
1349 609 94
SexAI A'CCwGG_T
Cuts at: 0 1187 2052
Size: 1187 865
SfcI C'TryA_G
Cuts at: 0 208 217 1375 1452 1513 1945 2052
Size: 208 9 1158 77 61 432 107
Fragments arranged by size:
1158 432 208 107 77 61 9
SfoI GGC'GCC
Cuts at: 0 1410 2052
Size: 1410 642
SmlI C'TyrA_G
Cuts at: 0 1162 1600 2052
Size: 1162 438 452
Fragments arranged by size:
1162 452 438
StuI AGG'CCT
Cuts at: 0 1861 2052
Size: 1861 191
StyI C'CwwG_G
Cuts at: 0 1247 1538 2052
Size: 1247 291 514
Fragments arranged by size:
1247 514 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 943 2052
Size: 943 1109
TatI w'GTAC_w
Cuts at: 0 1316 1988 2052
Size: 1316 672 64
Fragments arranged by size:
1316 672 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 2052
Size: 1108 32 912
Fragments arranged by size:
1108 912 32
Tth111I GACn'n_nGTC
Cuts at: 0 72 240 256 2052
Size: 72 168 16 1796
Fragments arranged by size:
1796 168 72 16
XbaI T'CTAG_A
Cuts at: 0 858 2052
Size: 858 1194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 2052
Size: 321 1731
XmnI GAAnn'nnTTC
Cuts at: 0 1027 2052
Size: 1027 1025
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BcgI BclI
BglI BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI ClaI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MscI
MslI MspA1I NarI NcoI NspI PflMI PfoI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII SacI SapI
SexAI SfcI SfoI SmlI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BfrBI BglII BmrI BmtI Bpu10I BsaAI BsaBI
BsaWI BsiWI BsmI BspEI BsrFI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PsiI PspXI RsrII SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XhoI XmaI ZraI