Restriction map for
pFN21ASDA1052
(Linear) (Six-base) MAPSORT of: KIAA1052.seq Check: 4869 from: 1 to: 4365
KIAA1052.orf
With 182 enzymes: SgfI *
October 24, 2006 15:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1274 1614 1844 4365
Size: 1274 340 230 2521
Fragments arranged by size:
2521 1274 340 230
AatII G_ACGT'C
Cuts at: 0 531 4365
Size: 531 3834
Acc65I G'GTAC_C
Cuts at: 0 1409 4365
Size: 1409 2956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1499 2932 3055 3078 3131 3586 4365
Size: 1499 1433 123 23 53 455 779
Fragments arranged by size:
1499 1433 779 455 123 53 23
AflIII A'CryG_T
Cuts at: 0 4136 4248 4365
Size: 4136 112 117
Fragments arranged by size:
4136 117 112
AhdI GACnn_n'nnGTC
Cuts at: 0 1215 1356 3288 4365
Size: 1215 141 1932 1077
Fragments arranged by size:
1932 1215 1077 141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2530 4108 4365
Size: 2530 1578 257
Fragments arranged by size:
2530 1578 257
AlwNI CAG_nnn'CTG
Cuts at: 0 823 1747 1777 1869 2103 2259 2883 2946
Size: 823 924 30 92 234 156 624 63
Cuts at: 2946 3081 3132 4365
Size: 135 51 1233
Fragments arranged by size:
1233 924 823 624 234 156 135 92
63 51 30
ApaI G_GGCC'C
Cuts at: 0 1452 1501 1660 2691 4010 4365
Size: 1452 49 159 1031 1319 355
Fragments arranged by size:
1452 1319 1031 355 159 49
ApoI r'AATT_y
Cuts at: 0 83 91 3005 4365
Size: 83 8 2914 1360
Fragments arranged by size:
2914 1360 83 8
AvaI C'yCGr_G
Cuts at: 0 98 1501 1513 1816 3744 3893 4365
Size: 98 1403 12 303 1928 149 472
Fragments arranged by size:
1928 1403 472 303 149 98 12
BamHI G'GATC_C
Cuts at: 0 1680 3643 4365
Size: 1680 1963 722
Fragments arranged by size:
1963 1680 722
BanI G'GyrC_C
Cuts at: 0 1409 1832 2654 2750 3308 3422 3518 4365
Size: 1409 423 822 96 558 114 96 847
Fragments arranged by size:
1409 847 822 558 423 114 96 96
BanII G_rGCy'C
Cuts at: 0 514 601 1452 1490 1501 1660 2034 2504
Size: 514 87 851 38 11 159 374 470
Cuts at: 2504 2691 4010 4365
Size: 187 1319 355
Fragments arranged by size:
1319 851 514 470 374 355 187 159
87 38 11
BbeI G_GCGC'C
Cuts at: 0 2754 3426 4365
Size: 2754 672 939
Fragments arranged by size:
2754 939 672
BbsI GAAGACnn'nnnn_
Cuts at: 0 919 1346 3236 4365
Size: 919 427 1890 1129
Fragments arranged by size:
1890 1129 919 427
BbvCI CC'TCA_GC
Cuts at: 0 1561 2260 2621 3323 3820 4365
Size: 1561 699 361 702 497 545
Fragments arranged by size:
1561 702 699 545 497 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2203 2237 4365
Size: 2203 34 2128
Fragments arranged by size:
2203 2128 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 456 4365
Size: 456 3909
BglI GCCn_nnn'nGGC
Cuts at: 0 2833 4365
Size: 2833 1532
BglII A'GATC_T
Cuts at: 0 3186 4365
Size: 3186 1179
BlpI GC'TnA_GC
Cuts at: 0 2078 2324 2503 2642 3775 3808 4096 4365
Size: 2078 246 179 139 1133 33 288 269
Fragments arranged by size:
2078 1133 288 269 246 179 139 33
Bme1580I G_kGCm'C
Cuts at: 0 741 1452 1501 1660 2657 2691 3634 4010
Size: 741 711 49 159 997 34 943 376
Cuts at: 4010 4365
Size: 355
Fragments arranged by size:
997 943 741 711 376 355 159 49
34
BmgBI CAC'GTC
Cuts at: 0 3974 4365
Size: 3974 391
BmrI ACTGGGnnnn_n'
Cuts at: 0 409 751 1285 1339 4365
Size: 409 342 534 54 3026
Fragments arranged by size:
3026 534 409 342 54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1478 1785 2391 4365
Size: 1478 307 606 1974
Fragments arranged by size:
1974 1478 606 307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 837 1420 1564 1709 2194 2326 2350
Size: 197 640 583 144 145 485 132 24
Cuts at: 2350 2361 2368 2692 2725 2764 2863 2968 3061
Size: 11 7 324 33 39 99 105 93
Cuts at: 3061 3124 3523 4138 4365
Size: 63 399 615 227
Fragments arranged by size:
640 615 583 485 399 324 227 197
145 144 132 105 99 93 63 39
33 24 11 7
Bpu10I CC'TnA_GC
Cuts at: 0 1561 2033 2260 2597 2621 3323 3694 3820
Size: 1561 472 227 337 24 702 371 126
Cuts at: 3820 4365
Size: 545
Fragments arranged by size:
1561 702 545 472 371 337 227 126
24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 684 1968 2167 2758 4365
Size: 550 134 1284 199 591 1607
Fragments arranged by size:
1607 1284 591 550 199 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 52 365 568 595 2379 2851 3505 4365
Size: 52 313 203 27 1784 472 654 860
Fragments arranged by size:
1784 860 654 472 313 203 52 27
BsaAI yAC'GTr
Cuts at: 0 2481 4365
Size: 2481 1884
BsaHI Gr'CG_yC
Cuts at: 0 528 1142 2751 3423 4365
Size: 528 614 1609 672 942
Fragments arranged by size:
1609 942 672 614 528
BsaWI w'CCGG_w
Cuts at: 0 2662 4232 4365
Size: 2662 1570 133
Fragments arranged by size:
2662 1570 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 1393 1423 1867 1897 3916 3946 4365
Size: 29 1364 30 444 30 2019 30 419
Fragments arranged by size:
2019 1364 444 419 30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 682 778 805 1081 1415 1417 1456
Size: 673 9 96 27 276 334 2 39
Cuts at: 1456 1546 1663 1702 1906 1915 1990 1993 1996
Size: 90 117 39 204 9 75 3 3
Cuts at: 1996 2014 2334 2431 2568 2590 2638 2869 2965
Size: 18 320 97 137 22 48 231 96
Cuts at: 2965 3304 3592 3853 4011 4365
Size: 339 288 261 158 354
Fragments arranged by size:
673 354 339 334 320 288 276 261
231 204 158 137 117 97 96 96
90 75 48 39 39 27 22 18
9 9 3 3 2
BseYI C'CCAG_C
Cuts at: 0 1319 1653 2423 2485 3410 3881 4128 4323
Size: 1319 334 770 62 925 471 247 195
Cuts at: 4323 4365
Size: 42
Fragments arranged by size:
1319 925 770 471 334 247 195 62
42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 884 1763 2914 3382 4365
Size: 884 879 1151 468 983
Fragments arranged by size:
1151 983 884 879 468
BsiHKAI G_wGCw'C
Cuts at: 0 514 1265 2034 2318 2456 2498 2504 2582
Size: 514 751 769 284 138 42 6 78
Cuts at: 2582 2969 3910 4340 4365
Size: 387 941 430 25
Fragments arranged by size:
941 769 751 514 430 387 284 138
78 42 25 6
BsmI GAATG_Cn'
Cuts at: 0 1635 2235 4365
Size: 1635 600 2130
Fragments arranged by size:
2130 1635 600
Bsp1286I G_dGCh'C
Cuts at: 0 514 601 741 1265 1452 1490 1501 1660
Size: 514 87 140 524 187 38 11 159
Cuts at: 1660 2034 2318 2456 2498 2504 2582 2657 2691
Size: 374 284 138 42 6 78 75 34
Cuts at: 2691 2969 3634 3910 4010 4340 4365
Size: 278 665 276 100 330 25
Fragments arranged by size:
665 524 514 374 330 284 278 276
187 159 140 138 100 87 78 75
42 38 34 25 11 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1274 1614 1844 2892 3569 4365
Size: 1274 340 230 1048 677 796
Fragments arranged by size:
1274 1048 796 677 340 230
BsrBI CCG'CTC
Cuts at: 0 297 1446 1956 2334 3861 4365
Size: 297 1149 510 378 1527 504
Fragments arranged by size:
1527 1149 510 504 378 297
BsrFI r'CCGG_y
Cuts at: 0 826 2753 4365
Size: 826 1927 1612
Fragments arranged by size:
1927 1612 826
BssSI C'ACGA_G
Cuts at: 0 1438 2497 4365
Size: 1438 1059 1868
Fragments arranged by size:
1868 1438 1059
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1869 2883 4365
Size: 1869 1014 1482
Fragments arranged by size:
1869 1482 1014
BstEII G'GTnAC_C
Cuts at: 0 981 4268 4365
Size: 981 3287 97
Fragments arranged by size:
3287 981 97
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1398 3522 4116 4365
Size: 1398 2124 594 249
Fragments arranged by size:
2124 1398 594 249
BstYI r'GATC_y
Cuts at: 0 487 1253 1680 1904 3054 3186 3643 3764
Size: 487 766 427 224 1150 132 457 121
Cuts at: 3764 4365
Size: 601
Fragments arranged by size:
1150 766 601 487 457 427 224 132
121
Bsu36I CC'TnA_GG
Cuts at: 0 1685 3205 4190 4365
Size: 1685 1520 985 175
Fragments arranged by size:
1685 1520 985 175
BtgI C'CryG_G
Cuts at: 0 1224 1790 3768 4365
Size: 1224 566 1978 597
Fragments arranged by size:
1978 1224 597 566
BtsI GCAGTG_nn'
Cuts at: 0 169 888 1014 1808 3705 3819 4365
Size: 169 719 126 794 1897 114 546
Fragments arranged by size:
1897 794 719 546 169 126 114
EaeI y'GGCC_r
Cuts at: 0 1472 3434 3452 3539 3938 4365
Size: 1472 1962 18 87 399 427
Fragments arranged by size:
1962 1472 427 399 87 18
EarI CTCTTCn'nnn_
Cuts at: 0 485 946 1079 1121 1478 1664 1790 1892
Size: 485 461 133 42 357 186 126 102
Cuts at: 1892 2105 2183 2203 2908 3608 4282 4287 4365
Size: 213 78 20 705 700 674 5 78
Fragments arranged by size:
705 700 674 485 461 357 213 186
133 126 102 78 78 42 20 5
EcoICRI GAG'CTC
Cuts at: 0 512 2032 2502 4365
Size: 512 1520 470 1863
Fragments arranged by size:
1863 1520 512 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 837 1420 1499 1564 1709 2194 2326
Size: 197 640 583 79 65 145 485 132
Cuts at: 2326 2350 2361 2368 2692 2725 2764 2863 2932
Size: 24 11 7 324 33 39 99 69
Cuts at: 2932 2968 3055 3061 3078 3124 3131 3523 3586
Size: 36 87 6 17 46 7 392 63
Cuts at: 3586 4138 4365
Size: 552 227
Fragments arranged by size:
640 583 552 485 392 324 227 197
145 132 99 87 79 69 65 63
46 39 36 33 24 17 11 7
7 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 449 3505 4365
Size: 449 3056 860
Fragments arranged by size:
3056 860 449
EcoO109I rG'GnC_Cy
Cuts at: 0 435 1497 1498 1593 1657 2687 3201 3477
Size: 435 1062 1 95 64 1030 514 276
Cuts at: 3477 3897 4006 4365
Size: 420 109 359
Fragments arranged by size:
1062 1030 514 435 420 359 276 109
95 64 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 103 609 3288 4365
Size: 103 506 2679 1077
Fragments arranged by size:
2679 1077 506 103
FspI TGC'GCA
Cuts at: 0 3443 3493 4365
Size: 3443 50 872
Fragments arranged by size:
3443 872 50
HaeII r_GCGC'y
Cuts at: 0 2529 2679 2754 3426 4365
Size: 2529 150 75 672 939
Fragments arranged by size:
2529 939 672 150 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 143 175 1201 1233 1342 1374 1720
Size: 31 112 32 1026 32 109 32 346
Cuts at: 1720 1752 2021 2053 2104 2136 3274 3306 3520
Size: 32 269 32 51 32 1138 32 214
Cuts at: 3520 3552 4365
Size: 32 813
Fragments arranged by size:
1138 1026 813 346 269 214 112 109
51 32 32 32 32 32 32 32
32 31
HincII GTy'rAC
Cuts at: 0 311 4365
Size: 311 4054
KasI G'GCGC_C
Cuts at: 0 2750 3422 4365
Size: 2750 672 943
Fragments arranged by size:
2750 943 672
KpnI G_GTAC'C
Cuts at: 0 1413 4365
Size: 1413 2952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2096 2540 2774 3575 4034 4365
Size: 2096 444 234 801 459 331
Fragments arranged by size:
2096 801 459 444 331 234
MscI TGG'CCA
Cuts at: 0 1474 3436 3454 3940 4365
Size: 1474 1962 18 486 425
Fragments arranged by size:
1962 1474 486 425 18
MslI CAynn'nnrTG
Cuts at: 0 260 3524 4365
Size: 260 3264 841
Fragments arranged by size:
3264 841 260
MspA1I CmG'CkG
Cuts at: 0 33 1572 2259 2271 2427 2637 2883 2946
Size: 33 1539 687 12 156 210 246 63
Cuts at: 2946 3807 4092 4116 4323 4365
Size: 861 285 24 207 42
Fragments arranged by size:
1539 861 687 285 246 210 207 156
63 42 33 24 12
NaeI GCC'GGC
Cuts at: 0 2755 4365
Size: 2755 1610
NarI GG'CG_CC
Cuts at: 0 2751 3423 4365
Size: 2751 672 942
Fragments arranged by size:
2751 942 672
NcoI C'CATG_G
Cuts at: 0 1224 1790 4365
Size: 1224 566 2575
Fragments arranged by size:
2575 1224 566
NgoMIV G'CCGG_C
Cuts at: 0 2753 4365
Size: 2753 1612
NspI r_CATG'y
Cuts at: 0 4140 4365
Size: 4140 225
PasI CC'CwG_GG
Cuts at: 0 1494 1660 4365
Size: 1494 166 2705
Fragments arranged by size:
2705 1494 166
PciI A'CATG_T
Cuts at: 0 4136 4365
Size: 4136 229
PflMI CCAn_nnn'nTGG
Cuts at: 0 175 1155 1230 2535 3432 3983 4365
Size: 175 980 75 1305 897 551 382
Fragments arranged by size:
1305 980 897 551 382 175 75
PfoI T'CCnGG_A
Cuts at: 0 1075 2780 2849 3827 4365
Size: 1075 1705 69 978 538
Fragments arranged by size:
1705 1075 978 538 69
PmlI CAC'GTG
Cuts at: 0 2481 4365
Size: 2481 1884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1172 1204 4365
Size: 1172 32 3161
Fragments arranged by size:
3161 1172 32
PpuMI rG'GwC_Cy
Cuts at: 0 3201 3897 4365
Size: 3201 696 468
Fragments arranged by size:
3201 696 468
PshAI GACnn'nnGTC
Cuts at: 0 872 4365
Size: 872 3493
PspOMI G'GGCC_C
Cuts at: 0 1448 1497 1656 2687 4006 4365
Size: 1448 49 159 1031 1319 359
Fragments arranged by size:
1448 1319 1031 359 159 49
PspXI vC'TCGA_Gb
Cuts at: 0 1816 4365
Size: 1816 2549
PstI C_TGCA'G
Cuts at: 0 1379 1554 1571 1964 2099 2255 2432 2684
Size: 1379 175 17 393 135 156 177 252
Cuts at: 2684 3023 3065 3086 4322 4365
Size: 339 42 21 1236 43
Fragments arranged by size:
1379 1236 393 339 252 177 175 156
135 43 42 21 17
PvuII CAG'CTG
Cuts at: 0 33 1572 2259 2271 2427 2883 2946 3807
Size: 33 1539 687 12 156 456 63 861
Cuts at: 3807 4323 4365
Size: 516 42
Fragments arranged by size:
1539 861 687 516 456 156 63 42
33 12
SacI G_AGCT'C
Cuts at: 0 514 2034 2504 4365
Size: 514 1520 470 1861
Fragments arranged by size:
1861 1520 514 470
SanDI GG'GwC_CC
Cuts at: 0 3897 4365
Size: 3897 468
SapI GCTCTTCn'nnn_
Cuts at: 0 485 1478 2203 4365
Size: 485 993 725 2162
Fragments arranged by size:
2162 993 725 485
ScaI AGT'ACT
Cuts at: 0 1814 4365
Size: 1814 2551
SexAI A'CCwGG_T
Cuts at: 0 921 4365
Size: 921 3444
SfcI C'TryA_G
Cuts at: 0 1001 1375 1550 1567 1960 2095 2251 2428
Size: 1001 374 175 17 393 135 156 177
Cuts at: 2428 2680 3019 3061 3082 4165 4318 4365
Size: 252 339 42 21 1083 153 47
Fragments arranged by size:
1083 1001 393 374 339 252 177 175
156 153 135 47 42 21 17
SfoI GGC'GCC
Cuts at: 0 2752 3424 4365
Size: 2752 672 941
Fragments arranged by size:
2752 941 672
SmaI CCC'GGG
Cuts at: 0 100 3895 4365
Size: 100 3795 470
Fragments arranged by size:
3795 470 100
SmlI C'TyrA_G
Cuts at: 0 565 699 1816 1947 2182 2737 4365
Size: 565 134 1117 131 235 555 1628
Fragments arranged by size:
1628 1117 565 555 235 134 131
StuI AGG'CCT
Cuts at: 0 1397 3619 4365
Size: 1397 2222 746
Fragments arranged by size:
2222 1397 746
StyI C'CwwG_G
Cuts at: 0 395 989 1094 1224 1790 2439 3216 3347
Size: 395 594 105 130 566 649 777 131
Cuts at: 3347 4365
Size: 1018
Fragments arranged by size:
1018 777 649 594 566 395 131 130
105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1288 2950 2986 3984 3994 4305 4365
Size: 1288 1662 36 998 10 311 60
Fragments arranged by size:
1662 1288 998 311 60 36 10
TatI w'GTAC_w
Cuts at: 0 1812 4365
Size: 1812 2553
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1867 1899 4365
Size: 1867 32 2466
Fragments arranged by size:
2466 1867 32
Tth111I GACn'n_nGTC
Cuts at: 0 923 4365
Size: 923 3442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 137 387 1152 1746 4365
Size: 137 250 765 594 2619
Fragments arranged by size:
2619 765 594 250 137
XhoI C'TCGA_G
Cuts at: 0 1816 4365
Size: 1816 2549
XmaI C'CCGG_G
Cuts at: 0 98 3893 4365
Size: 98 3795 472
Fragments arranged by size:
3795 472 98
XmnI GAAnn'nnTTC
Cuts at: 0 3091 4365
Size: 3091 1274
ZraI GAC'GTC
Cuts at: 0 529 4365
Size: 529 3836
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AhdI AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuII SacI SanDI SapI
ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AleI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BclI BfrBI BmtI
BsaBI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoRI EcoRV FseI FspAI HindIII
HpaI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PmeI PsiI PsrI PvuI RsrII SacII SalI
SbfI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI