Restriction map for
pFN21ASDA1029
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I AocI ApaLI AscI AseI
AsnI Asp700I AviII AvrII BbrPI
BcgI BclI BfrI BglII BlnI
BsaAI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI EclXI Eco52I Eco81I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI MfeI MluI MroI
MseI MunI NaeI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 661 GTCTAC GT^[AC][GT]AC
AcsI 348 GAATTC [GA]^AATT[CT]
AflIII 2576 ACGTGT A^C[GA][CT]GT
AhdI 1042 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2201 AGCGCT AGC^GCT
ApaI 2115 GGGCCC GGGCC^C
ApoI 348 GAATTC [GA]^AATT[CT]
AspEI 1042 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1444 GAGCTC G[AT]GC[AT]^C
AspI 969 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 731 GGATCC G^GATCC
BciVI 1029 GTATCC GTATCC(6/5)
BlpI 1441 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1441 GCTGAGC GC^T[ACGT]AGC
BsaBI 283 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2317 CGGTCG CG[GA][CT]^CG
BsiHKAI 1444 GAGCTC G[AT]GC[AT]^C
BsrBI 2556 CCGCTC CCGCTC(-3/-3)
BstEII 1164 GGTGACC G^GT[ACGT]ACC
BstPI 1164 GGTGACC G^GT[ACGT]ACC
CelII 1441 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1042 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 64 GAAGAG CTCTTC(1/4)
Eco47III 2201 AGCGCT AGC^GCT
EcoO65I 1164 GGTGACC G^GT[ACGT]ACC
EcoRI 348 GAATTC G^AATTC
Ksp632I 64 GAAGAG CTCTTC(1/4)
MaeII 2576 ACGT A^CGT
MamI 283 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 22 GCTAGC G^CTAGC
NspI 2402 GCATGC [GA]CATG^[CT]
PflFI 969 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1658 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 1444 GAGCTC GAGCT^C
SexAI 1790 ACCTGGT A^CC[AT]GGT
SphI 2402 GCATGC GCATG^C
TfiI 1173 GAATC G^A[AT]TC
Tth111I 969 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 69 GGTACC G^GTACC
1344 GGTACC
AlwNI 319 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
383 CAGGGCCTG
Asp718I 69 GGTACC G^GTACC
1344 GGTACC
BalI 50 TGGCCA TGG^CCA
686 TGGCCA
BbsI 1050 GTCTTC GAAGAC(2/6)
1491 GTCTTC
BbvCI 178 GCTGAGG CCTCAGC(-5/-2)
439 GCTGAGG
BpuAI 1050 GTCTTC GAAGAC(2/6)
1491 GTCTTC
BsmBI 783 GAGACG CGTCTC(1/5)
924 GAGACG
BsmI 96 GAATGC GAATGC(1/-1)
1094 GCATTC
BspMI 1643 ACCTGC ACCTGC(4/8)
1802 ACCTGC
BssSI 1307 CTCGTG CACGAG(-5/-1)
2190 CTCGTG
BstXI 752 CCAGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1778 CCAGCTCCTTGG
BstYI 731 GGATCC [GA]^GATC[CT]
1188 GGATCT
DraIII 405 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1304 CACCTCGTG
HincII 205 GTCAAC GT[CT]^[GA]AC
706 GTCAAC
HindII 205 GTCAAC GT[CT]^[GA]AC
706 GTCAAC
KpnI 69 GGTACC GGTAC^C
1344 GGTACC
MflI 731 GGATCC [GA]^GATC[CT]
1188 GGATCT
MluNI 50 TGGCCA TGG^CCA
686 TGGCCA
MscI 50 TGGCCA TGG^CCA
686 TGGCCA
MslI 597 CACCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1902 CACTGCCGTG
NcoI 878 CCATGG C^CATGG
1635 CCATGG
PflMI 370 CCATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1952 CCAGGGCGTGG
SmaI 1576 CCCGGG CCC^GGG
2112 CCCGGG
SmlI 1366 CTCAAG C^T[CT][GA]AG
2668 CTCAAG
Tsp509I 349 AATT ^AATT
468 AATT
Van91I 370 CCATCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1952 CCAGGGCGTGG
XcmI 593 CCAACACCCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
977 CCACAGCCCCTCTGG
XhoII 731 GGATCC [GA]^GATC[CT]
1188 GGATCT
XmaCI 1576 CCCGGG C^CCGGG
2112 CCCGGG
XmaI 1576 CCCGGG C^CCGGG
2112 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BanI BanII
BbeI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI KspI MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I