(Circular) (Six-base) MAPSORT of: pFN21ASDA1014.seq Check: 9325 from: 1 to: 7780 pFN21ASDA1014 7780 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4636 4636 Size: 7780 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5590 278 Size: 53 83 186 936 4054 2468 Fragments arranged by size: 4054 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5123 1937 Size: 3186 4594 Acc65I G'GTAC_C Cuts at: 2044 5101 2044 Size: 3057 4723 AclI AA'CG_TT Cuts at: 1563 5907 6280 1563 Size: 4344 373 3063 Fragments arranged by size: 4344 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2214 3752 4094 4296 5066 5843 7227 7495 Size: 582 1538 342 202 770 777 1384 268 Cuts at: 7495 1632 Size: 1917 Fragments arranged by size: 1917 1538 1384 777 770 582 342 268 202 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7583 AflIII A'CryG_T Cuts at: 1203 6679 7656 1203 Size: 5476 977 1327 Fragments arranged by size: 5476 1327 977 AgeI A'CCGG_T Cuts at: 6611 6611 Size: 7780 AhdI GACnn_n'nnGTC Cuts at: 6509 6509 Size: 7780 AleI CACnn'nnGTG Cuts at: 1166 2195 4418 1166 Size: 1029 2223 4528 Fragments arranged by size: 4528 2223 1029 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7780 AlwNI CAG_nnn'CTG Cuts at: 2510 2822 4628 4978 5523 7095 2510 Size: 312 1806 350 545 1572 3195 Fragments arranged by size: 3195 1806 1572 545 350 312 ApaLI G'TGCA_C Cuts at: 5837 6993 5837 Size: 1156 6624 ApoI r'AATT_y Cuts at: 1493 2943 3493 3514 3602 3641 4000 5089 5315 Size: 1450 550 21 88 39 359 1089 226 Cuts at: 5315 5467 7576 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1450 1089 550 359 226 152 88 39 21 AseI AT'TA_AT Cuts at: 160 1032 3660 6334 160 Size: 872 2628 2674 1606 Fragments arranged by size: 2674 2628 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7780 AvaI C'yCGr_G Cuts at: 1870 1943 2037 2088 4701 5105 1870 Size: 73 94 51 2613 404 4545 Fragments arranged by size: 4545 2613 404 94 73 51 BamHI G'GATC_C Cuts at: 5110 5477 5110 Size: 367 7413 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2044 5101 6556 618 Size: 325 217 627 257 3057 1455 1842 Fragments arranged by size: 3057 1842 1455 627 325 257 217 BanII G_rGCy'C Cuts at: 1391 3739 4272 5099 1391 Size: 2348 533 827 4072 Fragments arranged by size: 4072 2348 827 533 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3168 928 Size: 971 1269 5540 Fragments arranged by size: 5540 1269 971 BbvCI CC'TCA_GC Cuts at: 2496 2496 Size: 7780 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5971 6005 1903 Size: 34 4034 34 3678 Fragments arranged by size: 4034 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4889 5672 6882 4889 Size: 783 1210 5787 Fragments arranged by size: 5787 1210 783 BclI T'GATC_A Cuts at: 1548 1548 Size: 7780 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2177 2210 6391 Size: 107 122 71 631 605 505 33 4181 Cuts at: 6391 136 Size: 1525 Fragments arranged by size: 4181 1525 631 605 505 122 107 71 33 BglII A'GATC_T Cuts at: 7775 7775 Size: 7780 BlpI GC'TnA_GC Cuts at: 5197 5197 Size: 7780 Bme1580I G_kGCm'C Cuts at: 1790 5841 6997 1790 Size: 4051 1156 2573 Fragments arranged by size: 4051 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7780 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2799 3844 5511 Size: 416 53 83 399 24 1382 1045 1667 Cuts at: 5511 6469 442 Size: 958 1753 Fragments arranged by size: 1753 1667 1382 1045 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7780 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2158 2158 Size: 7780 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3222 3263 3444 3531 4161 4437 6440 1239 Size: 1983 41 181 87 630 276 2003 2579 Fragments arranged by size: 2579 2003 1983 630 276 181 87 41 Bpu10I CC'TnA_GC Cuts at: 2496 2496 Size: 7780 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3333 5905 6770 7068 3333 Size: 2572 865 298 4045 Fragments arranged by size: 4045 2572 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6443 882 Size: 617 4944 2219 Fragments arranged by size: 4944 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5077 5571 493 Size: 711 3873 494 2702 Fragments arranged by size: 3873 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7774 7774 Size: 7780 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5587 5969 6604 275 Size: 53 83 186 936 4054 382 635 1451 Fragments arranged by size: 4054 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6213 6611 6885 7032 7443 6213 Size: 398 274 147 411 6550 Fragments arranged by size: 6550 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3424 3454 1178 Size: 30 165 30 2021 30 5504 Fragments arranged by size: 5504 2021 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2484 2981 2984 3083 3227 4188 4200 4280 Size: 1298 497 3 99 144 961 12 80 Cuts at: 4280 4740 4743 4746 4749 4752 1186 Size: 460 3 3 3 3 4214 Fragments arranged by size: 4214 1298 961 497 460 144 99 80 12 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2159 6983 7479 2159 Size: 4824 496 2460 Fragments arranged by size: 4824 2460 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 4926 1163 Size: 573 543 2647 4017 Fragments arranged by size: 4017 2647 573 543 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2053 5230 5991 7019 1540 Size: 275 186 52 3177 761 1028 2301 Fragments arranged by size: 3177 2301 1028 761 275 186 52 BsiHKAI G_wGCw'C Cuts at: 5841 5926 6997 5841 Size: 85 1071 6624 Fragments arranged by size: 6624 1071 85 BsmI GAATG_Cn' Cuts at: 3363 5291 5384 3363 Size: 1928 93 5759 Fragments arranged by size: 5759 1928 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3739 4272 5099 5841 5926 6997 1391 Size: 399 1949 533 827 742 85 1071 2174 Fragments arranged by size: 2174 1949 1071 827 742 533 399 85 BspEI T'CCGG_A Cuts at: 7443 7443 Size: 7780 BspHI T'CATG_A Cuts at: 2301 5669 7399 2301 Size: 3368 1730 2682 Fragments arranged by size: 3368 2682 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4636 5135 844 Size: 3792 499 3489 Fragments arranged by size: 3792 3489 499 BsrBI CCG'CTC Cuts at: 1394 1823 5667 1394 Size: 429 3844 3507 Fragments arranged by size: 3844 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4682 6275 6449 65 Size: 1183 3434 1593 174 1396 Fragments arranged by size: 3434 1593 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6424 6611 1700 Size: 110 4614 187 2869 Fragments arranged by size: 4614 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7780 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7780 BssSI C'ACGA_G Cuts at: 5840 6852 5840 Size: 1012 6768 BstAPI GCAn_nnn'nTGC Cuts at: 794 3485 4628 794 Size: 2691 1143 3946 Fragments arranged by size: 3946 2691 1143 BstBI TT'CG_AA Cuts at: 2007 6584 2007 Size: 4577 3203 BstEII G'GTnAC_C Cuts at: 1184 4487 1184 Size: 3303 4477 BstXI CCAn_nnnn'nTGG Cuts at: 2621 2621 Size: 7780 BstYI r'GATC_y Cuts at: 1972 3178 4230 5110 5477 5862 5879 7331 7417 Size: 1206 1052 880 367 385 17 1452 86 Cuts at: 7417 7429 7775 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1206 1052 880 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3015 5025 3015 Size: 2010 5770 BtgI C'CryG_G Cuts at: 513 1065 2022 2148 2193 2208 513 Size: 552 957 126 45 15 6085 Fragments arranged by size: 6085 957 552 126 45 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2976 487 Size: 683 56 1750 5291 Fragments arranged by size: 5291 1750 683 56 BtsI GCAGTG_nn' Cuts at: 3193 4413 4425 5296 6090 6110 3193 Size: 1220 12 871 794 20 4863 Fragments arranged by size: 4863 1220 871 794 20 12 ClaI AT'CG_AT Cuts at: 1554 2478 1554 Size: 924 6856 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7745 DraI TTT'AAA Cuts at: 5084 5084 Size: 7780 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6787 809 Size: 507 5471 1802 Fragments arranged by size: 5471 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2050 2457 5227 Size: 54 1425 50 275 18 220 407 2770 Cuts at: 5227 6116 7745 8 Size: 889 1629 43 Fragments arranged by size: 2770 1629 1425 889 407 275 220 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2050 5227 1537 Size: 275 238 3177 4090 Fragments arranged by size: 4090 3177 275 238 EarI CTCTTCn'nnn_ Cuts at: 1353 3030 3213 3906 4829 4893 4917 4953 5042 Size: 1677 183 693 923 64 24 36 89 Cuts at: 5042 5710 1353 Size: 668 3423 Fragments arranged by size: 3423 1677 923 693 668 183 89 64 36 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2067 6367 6741 6887 387 Size: 1064 138 478 4300 374 146 1280 Fragments arranged by size: 4300 1280 1064 478 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 3222 3263 3444 3531 3752 4094 Size: 393 582 1008 41 181 87 221 342 Cuts at: 4094 4161 4296 4437 5066 5843 6440 7227 7495 Size: 67 135 141 629 777 597 787 268 Cuts at: 7495 1239 Size: 1524 Fragments arranged by size: 1524 1008 787 777 629 597 582 393 342 268 221 181 141 135 87 67 41 EcoNI CCTnn'n_nnAGG Cuts at: 2232 4278 5024 2232 Size: 2046 746 4988 Fragments arranged by size: 4988 2046 746 EcoO109I rG'GnC_Cy Cuts at: 2084 2110 2084 Size: 26 7754 EcoRI G'AATT_C Cuts at: 2943 3514 5089 5467 2943 Size: 571 1575 378 5256 Fragments arranged by size: 5256 1575 571 378 EcoRV GAT'ATC Cuts at: 3331 3331 Size: 7780 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3251 3755 3251 Size: 504 7276 FspI TGC'GCA Cuts at: 6286 6286 Size: 7780 HaeII r_GCGC'y Cuts at: 1678 6927 1678 Size: 5249 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3657 3689 3694 3726 4919 Size: 32 135 32 2085 32 5 32 1193 Cuts at: 4919 4951 6421 6453 6495 6527 1373 Size: 32 1470 32 42 32 2626 Fragments arranged by size: 2626 2085 1470 1193 135 42 32 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 2436 5124 5370 669 Size: 988 281 498 2688 246 3079 Fragments arranged by size: 3079 2688 988 498 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7780 HpaI GTT'AAC Cuts at: 5370 5370 Size: 7780 KpnI G_GTAC'C Cuts at: 2048 5105 2048 Size: 3057 4723 MfeI C'AATT_G Cuts at: 3480 4662 5379 3480 Size: 1182 717 5881 Fragments arranged by size: 5881 1182 717 MluI A'CGCG_T Cuts at: 7656 7656 Size: 7780 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2558 2641 3129 3702 6894 7078 1305 Size: 1253 83 488 573 3192 184 2007 Fragments arranged by size: 3192 2007 1253 573 488 184 83 MscI TGG'CCA Cuts at: 10 64 1489 1832 2459 10 Size: 54 1425 343 627 5331 Fragments arranged by size: 5331 1425 627 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2195 4418 5738 6097 6256 518 Size: 648 1029 2223 1320 359 159 2042 Fragments arranged by size: 2223 2042 1320 1029 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2150 2195 2210 2321 4978 5196 5873 7021 Size: 544 45 15 111 2657 218 677 1148 Cuts at: 7021 7266 1606 Size: 245 2120 Fragments arranged by size: 2657 2120 1148 677 544 245 218 111 45 15 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6271 Fragments arranged by size: 6271 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7780 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7780 NotI GC'GGCC_GC Cuts at: 5227 5227 Size: 7780 NspI r_CATG'y Cuts at: 1132 1229 5138 6683 1132 Size: 97 3909 1545 2229 Fragments arranged by size: 3909 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7780 PciI A'CATG_T Cuts at: 6679 6679 Size: 7780 PflMI CCAn_nnn'nTGG Cuts at: 1108 3467 1108 Size: 2359 5421 PfoI T'CCnGG_A Cuts at: 4233 4233 Size: 7780 PmeI GTTT'AAAC Cuts at: 5084 5084 Size: 7780 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5849 5881 7362 7394 5849 Size: 32 1481 32 6235 Fragments arranged by size: 6235 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2084 2110 2084 Size: 26 7754 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7780 PsiI TTA'TAA Cuts at: 5350 5350 Size: 7780 PstI C_TGCA'G Cuts at: 830 2506 4420 4599 5132 830 Size: 1676 1914 179 533 3478 Fragments arranged by size: 3478 1914 1676 533 179 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7780 PvuII CAG'CTG Cuts at: 2321 4978 2321 Size: 2657 5123 SacII CC_GC'GG Cuts at: 2151 2196 2211 2151 Size: 45 15 7720 Fragments arranged by size: 7720 45 15 SalI G'TCGA_C Cuts at: 1936 5122 1936 Size: 3186 4594 SanDI GG'GwC_CC Cuts at: 2110 2110 Size: 7780 SbfI CC_TGCA'GG Cuts at: 5132 5132 Size: 7780 ScaI AGT'ACT Cuts at: 6028 7740 6028 Size: 1712 6068 SfcI C'TryA_G Cuts at: 826 1045 2502 2511 4401 4416 4462 4476 4482 Size: 219 1457 9 1890 15 46 14 6 Cuts at: 4482 4595 5128 6263 6944 7135 826 Size: 113 533 1135 681 191 1471 Fragments arranged by size: 1890 1471 1457 1135 681 533 219 191 113 46 15 14 9 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7780 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7780 SmaI CCC'GGG Cuts at: 1872 2039 5107 1872 Size: 167 3068 4545 Fragments arranged by size: 4545 3068 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 3348 5884 6785 7047 820 Size: 197 926 1405 2536 901 262 1553 Fragments arranged by size: 2536 1553 1405 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5077 5571 493 Size: 4584 494 2702 Fragments arranged by size: 4584 2702 494 SpeI A'CTAG_T Cuts at: 152 2828 152 Size: 2676 5104 SphI G_CATG'C Cuts at: 1132 5138 1132 Size: 4006 3774 SspI AAT'ATT Cuts at: 5 52 5704 5 Size: 47 5652 2081 Fragments arranged by size: 5652 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7780 StyI C'CwwG_G Cuts at: 513 1065 2022 3282 4146 4609 5219 513 Size: 552 957 1260 864 463 610 3074 Fragments arranged by size: 3074 1260 957 864 610 552 463 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2627 5807 5824 5977 6162 6607 325 Size: 1255 1047 3180 17 153 185 445 1498 Fragments arranged by size: 3180 1498 1255 1047 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2450 3098 3562 Size: 274 80 33 51 1443 473 648 464 Cuts at: 3562 6026 7738 96 Size: 2464 1712 138 Fragments arranged by size: 2464 1712 1443 648 473 464 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7740 7772 7740 Size: 32 7748 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7780 XbaI T'CTAG_A Cuts at: 5116 5116 Size: 7780 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7780 XmaI C'CCGG_G Cuts at: 1870 2037 5105 1870 Size: 167 3068 4545 Fragments arranged by size: 4545 3068 167 XmnI GAAnn'nnTTC Cuts at: 2943 5019 5909 2943 Size: 2076 890 4814 Fragments arranged by size: 4814 2076 890 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5588 276 Size: 53 83 186 936 4054 2468 Fragments arranged by size: 4054 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI