(Circular) (Six-base) MAPSORT of: pFN21ASDA0989.seq Check: 8681 from: 1 to: 7069 pFN21ASDA0989 7069 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2902 3596 4250 2902 Size: 694 654 5721 Fragments arranged by size: 5721 694 654 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4879 278 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4412 1937 Size: 2475 4594 Acc65I G'GTAC_C Cuts at: 2712 4390 2712 Size: 1678 5391 AclI AA'CG_TT Cuts at: 1563 5196 5569 1563 Size: 3633 373 3063 Fragments arranged by size: 3633 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2495 2769 3944 5132 6516 6784 1632 Size: 863 274 1175 1188 1384 268 1917 Fragments arranged by size: 1917 1384 1188 1175 863 274 268 AfeI AGC'GCT Cuts at: 3968 3968 Size: 7069 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6872 AflIII A'CryG_T Cuts at: 1203 5968 6945 1203 Size: 4765 977 1327 Fragments arranged by size: 4765 1327 977 AgeI A'CCGG_T Cuts at: 5900 5900 Size: 7069 AhdI GACnn_n'nnGTC Cuts at: 5798 5798 Size: 7069 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7069 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2388 1184 Size: 1204 5865 AlwNI CAG_nnn'CTG Cuts at: 2137 2218 2815 3406 3700 3931 4812 6384 2137 Size: 81 597 591 294 231 881 1572 2822 Fragments arranged by size: 2822 1572 881 597 591 294 231 81 ApaI G_GGCC'C Cuts at: 2431 2545 2603 2618 2633 2431 Size: 114 58 15 15 6867 Fragments arranged by size: 6867 114 58 15 15 ApaLI G'TGCA_C Cuts at: 3588 5126 6282 3588 Size: 1538 1156 4375 Fragments arranged by size: 4375 1538 1156 ApoI r'AATT_y Cuts at: 1493 4378 4604 4756 6865 1493 Size: 2885 226 152 2109 1697 Fragments arranged by size: 2885 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5623 160 Size: 872 4591 1606 Fragments arranged by size: 4591 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 AvaI C'yCGr_G Cuts at: 1870 1943 2044 2431 2538 2640 2938 3432 3500 Size: 73 101 387 107 102 298 494 68 Cuts at: 3500 4394 1870 Size: 894 4545 Fragments arranged by size: 4545 894 494 387 298 107 102 101 73 68 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2642 2675 2642 Size: 33 7036 BamHI G'GATC_C Cuts at: 2757 4399 4766 2757 Size: 1642 367 5060 Fragments arranged by size: 5060 1642 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2534 2712 2773 3359 3608 Size: 325 217 627 747 178 61 586 249 Cuts at: 3608 4024 4390 5845 618 Size: 416 366 1455 1842 Fragments arranged by size: 1842 1455 747 627 586 416 366 325 249 217 178 61 BanII G_rGCy'C Cuts at: 1391 2174 2244 2431 2545 2561 2603 2618 2633 Size: 783 70 187 114 16 42 15 15 Cuts at: 2633 2882 3035 3651 4004 4077 4388 1391 Size: 249 153 616 353 73 311 4072 Fragments arranged by size: 4072 783 616 353 311 249 187 153 114 73 70 42 16 15 15 BbeI G_GCGC'C Cuts at: 2538 3363 2538 Size: 825 6244 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2043 3149 928 Size: 971 144 1106 4848 Fragments arranged by size: 4848 1106 971 144 BbvCI CC'TCA_GC Cuts at: 2471 2625 2929 2975 2471 Size: 154 304 46 6565 Fragments arranged by size: 6565 304 154 46 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5260 5294 1903 Size: 34 3323 34 3678 Fragments arranged by size: 3678 3323 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4961 6171 4961 Size: 1210 5859 BclI T'GATC_A Cuts at: 1548 3948 1548 Size: 2400 4669 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2384 2954 5680 Size: 107 122 71 631 605 712 570 2726 Cuts at: 5680 136 Size: 1525 Fragments arranged by size: 2726 1525 712 631 605 570 122 107 71 BglII A'GATC_T Cuts at: 7064 7064 Size: 7069 BlpI GC'TnA_GC Cuts at: 3650 3798 4486 3650 Size: 148 688 6233 Fragments arranged by size: 6233 688 148 Bme1580I G_kGCm'C Cuts at: 1790 2431 2484 2545 2603 2618 2633 2722 2920 Size: 641 53 61 58 15 15 89 198 Cuts at: 2920 2950 3592 3613 5130 6286 1790 Size: 30 642 21 1517 1156 2573 Fragments arranged by size: 2573 1517 1156 642 641 198 89 61 58 53 30 21 15 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7069 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2166 4800 5758 Size: 416 53 83 399 24 749 2634 958 Cuts at: 5758 442 Size: 1753 Fragments arranged by size: 2634 1753 958 749 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2120 1056 Size: 1064 6005 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2543 3047 3158 3353 3416 3560 3584 Size: 968 336 504 111 195 63 144 24 Cuts at: 3584 3627 3728 3953 3955 5729 1239 Size: 43 101 225 2 1774 2579 Fragments arranged by size: 2579 1774 968 504 336 225 195 144 111 101 63 43 24 2 Bpu10I CC'TnA_GC Cuts at: 2471 2625 2876 2929 2975 2471 Size: 154 251 53 46 6565 Fragments arranged by size: 6565 251 154 53 46 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3560 5194 6059 6357 3560 Size: 1634 865 298 4272 Fragments arranged by size: 4272 1634 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2678 3645 3972 5732 882 Size: 617 1179 967 327 1760 2219 Fragments arranged by size: 2219 1760 1179 967 617 327 BsaAI yAC'GTr Cuts at: 493 1204 2485 4366 4860 493 Size: 711 1281 1881 494 2702 Fragments arranged by size: 2702 1881 1281 711 494 BsaBI GATnn'nnATC Cuts at: 3217 4358 7063 3217 Size: 1141 2705 3223 Fragments arranged by size: 3223 2705 1141 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2535 3360 4104 4876 Size: 53 83 186 936 1002 825 744 772 Cuts at: 4876 5258 5893 275 Size: 382 635 1451 Fragments arranged by size: 1451 1002 936 825 772 744 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4252 5502 5900 6174 6321 6732 4252 Size: 1250 398 274 147 411 4589 Fragments arranged by size: 4589 1250 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 3722 3752 4220 Size: 30 165 30 1140 30 1149 30 468 Cuts at: 4220 4250 1178 Size: 30 3997 Fragments arranged by size: 3997 1149 1140 468 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2032 2342 2360 2551 2917 3109 3190 3458 Size: 846 310 18 191 366 192 81 268 Cuts at: 3458 3704 4160 1186 Size: 246 456 4095 Fragments arranged by size: 4095 846 456 366 310 268 246 192 191 81 18 BseYI C'CCAG_C Cuts at: 2315 2379 2546 3831 3842 6272 6768 2315 Size: 64 167 1285 11 2430 496 2616 Fragments arranged by size: 2616 2430 1285 496 167 64 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 4297 1163 Size: 573 306 2255 3935 Fragments arranged by size: 3935 2255 573 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4519 5280 6308 1540 Size: 275 186 2518 761 1028 2301 Fragments arranged by size: 2518 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 2561 2835 2882 3035 3592 3651 3918 4138 Size: 302 274 47 153 557 59 267 220 Cuts at: 4138 4208 5130 5215 6286 2259 Size: 70 922 85 1071 3042 Fragments arranged by size: 3042 1071 922 557 302 274 267 220 153 85 70 59 47 BsmI GAATG_Cn' Cuts at: 2989 4580 4673 2989 Size: 1591 93 5385 Fragments arranged by size: 5385 1591 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2096 2096 Size: 7069 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2244 2259 2431 2484 2545 2561 Size: 399 384 70 15 172 53 61 16 Cuts at: 2561 2603 2618 2633 2722 2835 2882 2920 2950 Size: 42 15 15 89 113 47 38 30 Cuts at: 2950 3035 3592 3613 3651 3918 4004 4077 4138 Size: 85 557 21 38 267 86 73 61 Cuts at: 4138 4208 4388 5130 5215 6286 1391 Size: 70 180 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 557 399 384 267 180 172 113 89 86 85 85 73 70 70 61 61 53 47 42 38 38 30 21 16 15 15 15 BspEI T'CCGG_A Cuts at: 6732 6732 Size: 7069 BspHI T'CATG_A Cuts at: 2680 4958 6688 2680 Size: 2278 1730 3061 Fragments arranged by size: 3061 2278 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2823 2902 3596 3894 4250 4424 844 Size: 1979 79 694 298 356 174 3489 Fragments arranged by size: 3489 1979 694 356 298 174 79 BsrBI CCG'CTC Cuts at: 1394 1823 3010 3484 4956 1394 Size: 429 1187 474 1472 3507 Fragments arranged by size: 3507 1472 1187 474 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3418 5564 5738 65 Size: 1183 2170 2146 174 1396 Fragments arranged by size: 2170 2146 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2298 3263 5713 5900 1700 Size: 110 488 965 2450 187 2869 Fragments arranged by size: 2869 2450 965 488 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7069 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7069 BssSI C'ACGA_G Cuts at: 5129 6141 5129 Size: 1012 6057 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7069 BstBI TT'CG_AA Cuts at: 2007 5873 2007 Size: 3866 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7069 BstYI r'GATC_y Cuts at: 1972 2190 2416 2757 3199 3696 3927 4399 4766 Size: 218 226 341 442 497 231 472 367 Cuts at: 4766 5151 5168 6620 6706 6718 7064 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 497 472 442 385 367 346 341 231 226 218 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2138 2138 Size: 7069 BtgI C'CryG_G Cuts at: 513 1065 2422 2708 2722 2866 3771 3786 513 Size: 552 1357 286 14 144 905 15 3796 Fragments arranged by size: 3796 1357 905 552 286 144 15 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6330 Fragments arranged by size: 6330 683 56 BtsI GCAGTG_nn' Cuts at: 2691 2950 3347 3631 3637 3951 4585 5379 5399 Size: 259 397 284 6 314 634 794 20 Cuts at: 5399 2691 Size: 4361 Fragments arranged by size: 4361 794 634 397 314 284 259 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7069 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7034 DraI TTT'AAA Cuts at: 4373 4373 Size: 7069 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6076 809 Size: 507 4760 1802 Fragments arranged by size: 4760 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2396 2863 3049 Size: 54 1425 50 275 18 566 467 186 Cuts at: 3049 3282 3877 4516 5405 7034 8 Size: 233 595 639 889 1629 43 Fragments arranged by size: 1629 1425 889 639 595 566 467 275 233 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4516 1537 Size: 275 2704 4090 Fragments arranged by size: 4090 2704 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3435 4084 4999 1353 Size: 2082 649 915 3423 Fragments arranged by size: 3423 2082 915 649 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5656 6030 6176 387 Size: 1064 138 4067 374 146 1280 Fragments arranged by size: 4067 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2559 2880 3033 3649 2559 Size: 321 153 616 5979 Fragments arranged by size: 5979 616 321 153 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2495 2543 2769 3047 3158 3353 Size: 393 575 288 48 226 278 111 195 Cuts at: 3353 3416 3560 3584 3627 3728 3944 3953 3955 Size: 63 144 24 43 101 216 9 2 Cuts at: 3955 5132 5729 6516 6784 1239 Size: 1177 597 787 268 1524 Fragments arranged by size: 1524 1177 787 597 575 393 288 278 268 226 216 195 144 111 101 63 48 43 24 9 2 EcoNI CCTnn'n_nnAGG Cuts at: 2069 3566 3935 2069 Size: 1497 369 5203 Fragments arranged by size: 5203 1497 369 EcoO109I rG'GnC_Cy Cuts at: 2134 2391 2427 2428 2458 2542 2599 2600 2614 Size: 257 36 1 30 84 57 1 14 Cuts at: 2614 2615 2629 2645 3133 3580 3955 2134 Size: 1 14 16 488 447 375 5248 Fragments arranged by size: 5248 488 447 375 257 84 57 36 30 16 14 14 1 1 1 EcoRI G'AATT_C Cuts at: 4378 4756 4378 Size: 378 6691 FspI TGC'GCA Cuts at: 3417 3452 5575 3417 Size: 35 2123 4911 Fragments arranged by size: 4911 2123 35 HaeII r_GCGC'y Cuts at: 1678 2538 3363 3500 3657 3707 3970 6216 1678 Size: 860 825 137 157 50 263 2246 2531 Fragments arranged by size: 2531 2246 860 825 263 157 137 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4220 4252 5710 5742 5784 Size: 32 135 32 2648 32 1458 32 42 Cuts at: 5784 5816 1373 Size: 32 2626 Fragments arranged by size: 2648 2626 1458 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4413 4659 669 Size: 988 281 2475 246 3079 Fragments arranged by size: 3079 2475 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7069 HpaI GTT'AAC Cuts at: 4659 4659 Size: 7069 KasI G'GCGC_C Cuts at: 2534 3359 2534 Size: 825 6244 KpnI G_GTAC'C Cuts at: 2716 4394 2716 Size: 1678 5391 MfeI C'AATT_G Cuts at: 4668 4668 Size: 7069 MluI A'CGCG_T Cuts at: 6945 6945 Size: 7069 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2423 2815 3844 6183 6367 1305 Size: 1118 392 1029 2339 184 2007 Fragments arranged by size: 2339 2007 1118 1029 392 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3284 3879 10 Size: 54 1425 343 1452 595 3200 Fragments arranged by size: 3200 1452 1425 595 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5027 5386 5545 518 Size: 648 3861 359 159 2042 Fragments arranged by size: 3861 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 2581 2724 2855 3082 3376 3508 3538 Size: 474 501 143 131 227 294 132 30 Cuts at: 3538 4485 5162 6310 6555 1606 Size: 947 677 1148 245 2120 Fragments arranged by size: 2120 1148 947 677 501 474 294 245 227 143 132 131 30 NaeI GCC'GGC Cuts at: 3265 3265 Size: 7069 NarI GG'CG_CC Cuts at: 2535 3360 2535 Size: 825 6244 NcoI C'CATG_G Cuts at: 513 1065 2866 3771 513 Size: 552 1801 905 3811 Fragments arranged by size: 3811 1801 905 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7069 NgoMIV G'CCGG_C Cuts at: 3263 3263 Size: 7069 NheI G'CTAG_C Cuts at: 1052 2116 1052 Size: 1064 6005 NotI GC'GGCC_GC Cuts at: 4516 4516 Size: 7069 NspI r_CATG'y Cuts at: 1132 1229 2405 2747 4427 5972 1132 Size: 97 1176 342 1680 1545 2229 Fragments arranged by size: 2229 1680 1545 1176 342 97 PasI CC'CwG_GG Cuts at: 1348 2245 4060 1348 Size: 897 1815 4357 Fragments arranged by size: 4357 1815 897 PciI A'CATG_T Cuts at: 5968 5968 Size: 7069 PflMI CCAn_nnn'nTGG Cuts at: 1108 3931 1108 Size: 2823 4246 PfoI T'CCnGG_A Cuts at: 2070 3930 3971 4329 2070 Size: 1860 41 358 4810 Fragments arranged by size: 4810 1860 358 41 PmeI GTTT'AAAC Cuts at: 4373 4373 Size: 7069 PmlI CAC'GTG Cuts at: 2485 2485 Size: 7069 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5138 5170 6651 6683 5138 Size: 32 1481 32 5524 Fragments arranged by size: 5524 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2391 2645 3955 2391 Size: 254 1310 5505 Fragments arranged by size: 5505 1310 254 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7069 PsiI TTA'TAA Cuts at: 4639 4639 Size: 7069 PspOMI G'GGCC_C Cuts at: 2427 2541 2599 2614 2629 2427 Size: 114 58 15 15 6867 Fragments arranged by size: 6867 114 58 15 15 PspXI vC'TCGA_Gb Cuts at: 3432 3432 Size: 7069 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2146 2178 2279 2311 2146 Size: 32 101 32 6904 Fragments arranged by size: 6904 101 32 32 PstI C_TGCA'G Cuts at: 830 2223 2766 2820 2997 3018 3492 3513 4421 Size: 1393 543 54 177 21 474 21 908 Cuts at: 4421 830 Size: 3478 Fragments arranged by size: 3478 1393 908 543 474 177 54 21 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7069 PvuII CAG'CTG Cuts at: 2080 2581 2855 3508 3538 2080 Size: 501 274 653 30 5611 Fragments arranged by size: 5611 653 501 274 30 SacI G_AGCT'C Cuts at: 2561 2882 3035 3651 2561 Size: 321 153 616 5979 Fragments arranged by size: 5979 616 321 153 SacII CC_GC'GG Cuts at: 2725 2725 Size: 7069 SalI G'TCGA_C Cuts at: 1936 4411 1936 Size: 2475 4594 SanDI GG'GwC_CC Cuts at: 2391 2391 Size: 7069 SbfI CC_TGCA'GG Cuts at: 2766 4421 2766 Size: 1655 5414 ScaI AGT'ACT Cuts at: 2369 5317 7029 2369 Size: 2948 1712 2409 Fragments arranged by size: 2948 2409 1712 SfcI C'TryA_G Cuts at: 826 1045 2219 2762 2816 2993 3014 3488 3509 Size: 219 1174 543 54 177 21 474 21 Cuts at: 3509 4122 4311 4417 5552 6233 6424 826 Size: 613 189 106 1135 681 191 1471 Fragments arranged by size: 1471 1174 1135 681 613 543 474 219 191 189 177 106 54 21 21 SfoI GGC'GCC Cuts at: 2536 3361 2536 Size: 825 6244 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7069 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7069 SmaI CCC'GGG Cuts at: 1872 2433 2540 4396 1872 Size: 561 107 1856 4545 Fragments arranged by size: 4545 1856 561 107 SmlI C'TyrA_G Cuts at: 820 1017 1943 3432 3575 5173 6074 6336 820 Size: 197 926 1489 143 1598 901 262 1553 Fragments arranged by size: 1598 1553 1489 926 901 262 197 143 SnaBI TAC'GTA Cuts at: 493 4366 4860 493 Size: 3873 494 2702 Fragments arranged by size: 3873 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7069 SphI G_CATG'C Cuts at: 1132 2747 4427 1132 Size: 1615 1680 3774 Fragments arranged by size: 3774 1680 1615 SrfI GCCC'GGGC Cuts at: 2540 2540 Size: 7069 SspI AAT'ATT Cuts at: 5 52 4993 5 Size: 47 4941 2081 Fragments arranged by size: 4941 2081 47 StuI AGG'CCT Cuts at: 1517 2180 2924 3464 1517 Size: 663 744 540 5122 Fragments arranged by size: 5122 744 663 540 StyI C'CwwG_G Cuts at: 513 1065 2866 3771 4508 513 Size: 552 1801 905 737 3074 Fragments arranged by size: 3074 1801 905 737 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2427 3856 4153 4223 5096 5113 5266 Size: 1255 847 1429 297 70 873 17 153 Cuts at: 5266 5451 5896 325 Size: 185 445 1498 Fragments arranged by size: 1498 1429 1255 873 847 445 297 185 153 70 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2367 2671 5315 Size: 274 80 33 51 1443 390 304 2644 Cuts at: 5315 7027 96 Size: 1712 138 Fragments arranged by size: 2644 1712 1443 390 304 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 7029 7061 2543 Size: 32 4454 32 2551 Fragments arranged by size: 4454 2551 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7069 XbaI T'CTAG_A Cuts at: 4405 4405 Size: 7069 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2029 Size: 7069 XhoI C'TCGA_G Cuts at: 1943 3432 1943 Size: 1489 5580 XmaI C'CCGG_G Cuts at: 1870 2431 2538 4394 1870 Size: 561 107 1856 4545 Fragments arranged by size: 4545 1856 561 107 XmnI GAAnn'nnTTC Cuts at: 5198 5198 Size: 7069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4877 276 Size: 53 83 186 936 3343 2468 Fragments arranged by size: 3343 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI RsrII SapI SexAI SfiI SwaI