(Circular) (Six-base) MAPSORT of: pFN21ASDA0976.seq Check: 4616 from: 1 to: 5821 pFN21ASDA0976 5821 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3631 278 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2663 3164 1937 Size: 726 501 4594 Fragments arranged by size: 4594 726 501 Acc65I G'GTAC_C Cuts at: 3142 3142 Size: 5821 AclI AA'CG_TT Cuts at: 1563 3948 4321 1563 Size: 2385 373 3063 Fragments arranged by size: 3063 2385 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3884 5268 5536 1632 Size: 2252 1384 268 1917 Fragments arranged by size: 2252 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5624 AflIII A'CryG_T Cuts at: 1203 2280 4720 5697 1203 Size: 1077 2440 977 1327 Fragments arranged by size: 2440 1327 1077 977 AgeI A'CCGG_T Cuts at: 4652 4652 Size: 5821 AhdI GACnn_n'nnGTC Cuts at: 4550 4550 Size: 5821 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5821 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5821 AlwNI CAG_nnn'CTG Cuts at: 3564 5136 3564 Size: 1572 4249 ApaLI G'TGCA_C Cuts at: 3878 5034 3878 Size: 1156 4665 ApoI r'AATT_y Cuts at: 1493 3130 3356 3508 5617 1493 Size: 1637 226 152 2109 1697 Fragments arranged by size: 2109 1697 1637 226 152 AseI AT'TA_AT Cuts at: 160 1032 4375 160 Size: 872 3343 1606 Fragments arranged by size: 3343 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 AvaI C'yCGr_G Cuts at: 1870 1943 3146 1870 Size: 73 1203 4545 Fragments arranged by size: 4545 1203 73 BamHI G'GATC_C Cuts at: 2587 3151 3518 2587 Size: 564 367 4890 Fragments arranged by size: 4890 564 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3142 4597 618 Size: 325 217 627 1355 1455 1842 Fragments arranged by size: 1842 1455 1355 627 325 217 BanII G_rGCy'C Cuts at: 1391 3140 1391 Size: 1749 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2362 928 Size: 971 463 4387 Fragments arranged by size: 4387 971 463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2892 2926 4012 4046 1903 Size: 34 955 34 1086 34 3678 Fragments arranged by size: 3678 1086 955 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2409 2758 3095 3713 4923 2409 Size: 349 337 618 1210 3307 Fragments arranged by size: 3307 1210 618 349 337 BclI T'GATC_A Cuts at: 1548 1548 Size: 5821 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4432 136 Size: 107 122 71 631 605 2760 1525 Fragments arranged by size: 2760 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5816 5816 Size: 5821 BlpI GC'TnA_GC Cuts at: 3238 3238 Size: 5821 Bme1580I G_kGCm'C Cuts at: 1790 3882 5038 1790 Size: 2092 1156 2573 Fragments arranged by size: 2573 2092 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5821 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3084 3552 4510 Size: 416 53 83 399 24 1667 468 958 Cuts at: 4510 442 Size: 1753 Fragments arranged by size: 1753 1667 958 468 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5821 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2396 2528 4481 1239 Size: 1157 132 1953 2579 Fragments arranged by size: 2579 1953 1157 132 Bpu10I CC'TnA_GC Cuts at: 2313 2331 2313 Size: 18 5803 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3946 4811 5109 3946 Size: 865 298 4658 Fragments arranged by size: 4658 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2235 4484 882 Size: 617 736 2249 2219 Fragments arranged by size: 2249 2219 736 617 BsaAI yAC'GTr Cuts at: 493 1204 3118 3612 493 Size: 711 1914 494 2702 Fragments arranged by size: 2702 1914 711 494 BsaBI GATnn'nnATC Cuts at: 5815 5815 Size: 5821 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2488 3628 4010 4645 Size: 53 83 186 936 955 1140 382 635 Cuts at: 4645 275 Size: 1451 Fragments arranged by size: 1451 1140 955 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2224 4254 4652 4926 5073 5484 2224 Size: 2030 398 274 147 411 2561 Fragments arranged by size: 2561 2030 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5596 Fragments arranged by size: 5596 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5821 BseYI C'CCAG_C Cuts at: 5024 5520 5024 Size: 496 5325 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5248 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3271 4032 5060 1540 Size: 275 186 1270 761 1028 2301 Fragments arranged by size: 2301 1270 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2979 3882 3967 5038 2979 Size: 903 85 1071 3762 Fragments arranged by size: 3762 1071 903 85 BsmI GAATG_Cn' Cuts at: 3332 3425 3332 Size: 93 5728 BsmBI CGTCTCn'nnnn_ Cuts at: 2246 2246 Size: 5821 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2979 3140 3882 3967 5038 1391 Size: 399 1189 161 742 85 1071 2174 Fragments arranged by size: 2174 1189 1071 742 399 161 85 BspEI T'CCGG_A Cuts at: 2224 5484 2224 Size: 3260 2561 BspHI T'CATG_A Cuts at: 3710 5440 3710 Size: 1730 4091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3176 844 Size: 2332 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3708 1394 Size: 429 1885 3507 Fragments arranged by size: 3507 1885 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2271 4316 4490 65 Size: 1183 1023 2045 174 1396 Fragments arranged by size: 2045 1396 1183 1023 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4465 4652 1700 Size: 110 2655 187 2869 Fragments arranged by size: 2869 2655 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5821 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5821 BssSI C'ACGA_G Cuts at: 3881 4893 3881 Size: 1012 4809 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5821 BstBI TT'CG_AA Cuts at: 2007 4625 2007 Size: 2618 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5821 BstYI r'GATC_y Cuts at: 1972 2587 3151 3518 3903 3920 5372 5458 5470 Size: 615 564 367 385 17 1452 86 12 Cuts at: 5470 5816 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 615 564 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2664 2664 Size: 5821 Bsu36I CC'TnA_GG Cuts at: 2807 2807 Size: 5821 BtgI C'CryG_G Cuts at: 513 1065 2184 513 Size: 552 1119 4150 Fragments arranged by size: 4150 1119 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5082 Fragments arranged by size: 5082 683 56 BtsI GCAGTG_nn' Cuts at: 3069 3337 4131 4151 3069 Size: 268 794 20 4739 Fragments arranged by size: 4739 794 268 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5821 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5786 DraI TTT'AAA Cuts at: 3125 3125 Size: 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3036 4828 809 Size: 507 1720 1792 1802 Fragments arranged by size: 1802 1792 1720 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3268 4157 5786 Size: 54 1425 50 275 18 1438 889 1629 Cuts at: 5786 8 Size: 43 Fragments arranged by size: 1629 1438 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3268 1537 Size: 275 1456 4090 Fragments arranged by size: 4090 1456 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2640 3751 1353 Size: 1287 1111 3423 Fragments arranged by size: 3423 1287 1111 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4408 4782 4928 387 Size: 1064 138 2819 374 146 1280 Fragments arranged by size: 2819 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2396 2528 3884 4481 5268 5536 1239 Size: 393 764 132 1356 597 787 268 1524 Fragments arranged by size: 1524 1356 787 764 597 393 268 132 EcoRI G'AATT_C Cuts at: 3130 3508 3130 Size: 378 5443 FspI TGC'GCA Cuts at: 4327 4327 Size: 5821 HaeII r_GCGC'y Cuts at: 1678 4968 1678 Size: 3290 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2185 2217 2579 2611 4462 Size: 32 135 32 613 32 362 32 1851 Cuts at: 4462 4494 4536 4568 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1851 613 362 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2419 3165 3411 669 Size: 988 281 481 746 246 3079 Fragments arranged by size: 3079 988 746 481 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5821 HpaI GTT'AAC Cuts at: 2419 3411 2419 Size: 992 4829 KpnI G_GTAC'C Cuts at: 3146 3146 Size: 5821 MfeI C'AATT_G Cuts at: 3420 3420 Size: 5821 MluI A'CGCG_T Cuts at: 5697 5697 Size: 5821 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4935 5119 1305 Size: 3630 184 2007 Fragments arranged by size: 3630 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3999 Fragments arranged by size: 3999 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2897 2968 3779 4138 4297 518 Size: 648 1023 708 71 811 359 159 2042 Fragments arranged by size: 2042 1023 811 708 648 359 159 71 MspA1I CmG'CkG Cuts at: 1606 2761 3237 3914 5062 5307 1606 Size: 1155 476 677 1148 245 2120 Fragments arranged by size: 2120 1155 1148 677 476 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5269 NdeI CA'TA_TG Cuts at: 387 387 Size: 5821 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5821 NotI GC'GGCC_GC Cuts at: 3268 3268 Size: 5821 NspI r_CATG'y Cuts at: 1132 1229 2284 2967 3179 4724 1132 Size: 97 1055 683 212 1545 2229 Fragments arranged by size: 2229 1545 1055 683 212 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5821 PciI A'CATG_T Cuts at: 2280 4720 2280 Size: 2440 3381 PflMI CCAn_nnn'nTGG Cuts at: 1108 2373 2834 2999 1108 Size: 1265 461 165 3930 Fragments arranged by size: 3930 1265 461 165 PfoI T'CCnGG_A Cuts at: 2505 2505 Size: 5821 PmeI GTTT'AAAC Cuts at: 3125 3125 Size: 5821 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3890 3922 5403 5435 3890 Size: 32 1481 32 4276 Fragments arranged by size: 4276 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5821 PsiI TTA'TAA Cuts at: 3391 3391 Size: 5821 PstI C_TGCA'G Cuts at: 830 3173 830 Size: 2343 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5821 PvuII CAG'CTG Cuts at: 2761 2761 Size: 5821 SalI G'TCGA_C Cuts at: 1936 3163 1936 Size: 1227 4594 SbfI CC_TGCA'GG Cuts at: 3173 3173 Size: 5821 ScaI AGT'ACT Cuts at: 2651 4069 5781 2651 Size: 1418 1712 2691 Fragments arranged by size: 2691 1712 1418 SfcI C'TryA_G Cuts at: 826 1045 2985 3169 4304 4985 5176 826 Size: 219 1940 184 1135 681 191 1471 Fragments arranged by size: 1940 1471 1135 681 219 191 184 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5821 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5821 SmaI CCC'GGG Cuts at: 1872 3148 1872 Size: 1276 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3925 4826 5088 820 Size: 197 926 1982 901 262 1553 Fragments arranged by size: 1982 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3118 3612 493 Size: 2625 494 2702 Fragments arranged by size: 2702 2625 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5821 SphI G_CATG'C Cuts at: 1132 3179 1132 Size: 2047 3774 SspI AAT'ATT Cuts at: 5 52 2844 3745 5 Size: 47 2792 901 2081 Fragments arranged by size: 2792 2081 901 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5821 StyI C'CwwG_G Cuts at: 513 1065 3032 3260 513 Size: 552 1967 228 3074 Fragments arranged by size: 3074 1967 552 228 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3848 3865 4018 4203 4648 325 Size: 1255 2268 17 153 185 445 1498 Fragments arranged by size: 2268 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2649 4067 5779 Size: 274 80 33 51 1443 672 1418 1712 Cuts at: 5779 96 Size: 138 Fragments arranged by size: 1712 1443 1418 672 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2974 3006 5781 5813 2974 Size: 32 2775 32 2982 Fragments arranged by size: 2982 2775 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5821 XbaI T'CTAG_A Cuts at: 3157 3157 Size: 5821 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5821 XmaI C'CCGG_G Cuts at: 1870 3146 1870 Size: 1276 4545 XmnI GAAnn'nnTTC Cuts at: 3950 3950 Size: 5821 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3629 276 Size: 53 83 186 936 2095 2468 Fragments arranged by size: 2468 2095 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI