(Circular) (Six-base) MAPSORT of: pFN21ASDA0969.seq Check: 199 from: 1 to: 7873 pFN21ASDA0969 7873 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5683 278 Size: 53 83 186 936 4147 2468 Fragments arranged by size: 4147 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4635 5166 5216 1937 Size: 2698 531 50 4594 Fragments arranged by size: 4594 2698 531 50 Acc65I G'GTAC_C Cuts at: 3004 5194 3004 Size: 2190 5683 AclI AA'CG_TT Cuts at: 1563 6000 6373 1563 Size: 4437 373 3063 Fragments arranged by size: 4437 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2419 2960 3036 5936 7320 7588 1632 Size: 787 541 76 2900 1384 268 1917 Fragments arranged by size: 2900 1917 1384 787 541 268 76 AfeI AGC'GCT Cuts at: 2165 2261 2165 Size: 96 7777 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7676 AflIII A'CryG_T Cuts at: 1203 2394 6772 7749 1203 Size: 1191 4378 977 1327 Fragments arranged by size: 4378 1327 1191 977 AgeI A'CCGG_T Cuts at: 6704 6704 Size: 7873 AhdI GACnn_n'nnGTC Cuts at: 6602 6602 Size: 7873 AleI CACnn'nnGTG Cuts at: 1166 4518 1166 Size: 3352 4521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7873 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4007 4039 4007 Size: 32 7841 AlwNI CAG_nnn'CTG Cuts at: 3736 5616 7188 3736 Size: 1880 1572 4421 Fragments arranged by size: 4421 1880 1572 ApaI G_GGCC'C Cuts at: 2792 3187 2792 Size: 395 7478 ApaLI G'TGCA_C Cuts at: 5930 7086 5930 Size: 1156 6717 ApoI r'AATT_y Cuts at: 1493 5182 5408 5560 7669 1493 Size: 3689 226 152 2109 1697 Fragments arranged by size: 3689 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6427 160 Size: 872 5395 1606 Fragments arranged by size: 5395 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7873 AvaI C'yCGr_G Cuts at: 1870 1943 2450 2493 2643 2783 3881 4026 4701 Size: 73 507 43 150 140 1098 145 675 Cuts at: 4701 5198 1870 Size: 497 4545 Fragments arranged by size: 4545 1098 675 507 497 150 145 140 73 43 BamHI G'GATC_C Cuts at: 2469 3291 3984 5203 5570 2469 Size: 822 693 1219 367 4772 Fragments arranged by size: 4772 1219 822 693 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3004 4512 4921 5138 5194 Size: 325 217 627 1217 1508 409 217 56 Cuts at: 5194 6649 618 Size: 1455 1842 Fragments arranged by size: 1842 1508 1455 1217 627 409 325 217 217 56 BanII G_rGCy'C Cuts at: 1391 2450 2792 2963 3187 3258 3413 4139 4160 Size: 1059 342 171 224 71 155 726 21 Cuts at: 4160 4197 4410 4743 4776 4844 5192 1391 Size: 37 213 333 33 68 348 4072 Fragments arranged by size: 4072 1059 726 348 342 333 224 213 171 155 71 68 37 33 21 BbeI G_GCGC'C Cuts at: 5142 5142 Size: 7873 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3024 928 Size: 971 1125 5777 Fragments arranged by size: 5777 1125 971 BbvCI CC'TCA_GC Cuts at: 4007 4069 4106 4007 Size: 62 37 7774 Fragments arranged by size: 7774 62 37 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2131 2165 6064 6098 1903 Size: 34 194 34 3899 34 3678 Fragments arranged by size: 3899 3678 194 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2330 5765 6975 2330 Size: 3435 1210 3228 Fragments arranged by size: 3435 3228 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7873 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2695 4282 4568 Size: 107 122 71 631 605 1023 1587 286 Cuts at: 4568 4664 4681 6484 136 Size: 96 17 1803 1525 Fragments arranged by size: 1803 1587 1525 1023 631 605 286 122 107 96 71 17 BglII A'GATC_T Cuts at: 3975 7868 3975 Size: 3893 3980 BlpI GC'TnA_GC Cuts at: 2406 5290 2406 Size: 2884 4989 Bme1580I G_kGCm'C Cuts at: 1790 2529 2792 3187 3393 3427 3460 4034 4278 Size: 739 263 395 206 34 33 574 244 Cuts at: 4278 4338 4926 4956 5934 7090 1790 Size: 60 588 30 978 1156 2573 Fragments arranged by size: 2573 1156 978 739 588 574 395 263 244 206 60 34 33 30 BmgBI CAC'GTC Cuts at: 1318 3994 4756 4951 1318 Size: 2676 762 195 4240 Fragments arranged by size: 4240 2676 762 195 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2907 3085 3447 Size: 416 53 83 399 24 1490 178 362 Cuts at: 3447 4575 5604 6562 442 Size: 1128 1029 958 1753 Fragments arranged by size: 1753 1490 1128 1029 958 416 399 362 178 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7873 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2776 4957 2776 Size: 2181 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2088 2493 3209 3758 4000 4670 4767 4841 Size: 849 405 716 549 242 670 97 74 Cuts at: 4841 5088 6533 1239 Size: 247 1445 2579 Fragments arranged by size: 2579 1445 849 716 670 549 405 247 242 97 74 Bpu10I CC'TnA_GC Cuts at: 2342 2619 2625 4007 4069 4106 4133 4838 2342 Size: 277 6 1382 62 37 27 705 5377 Fragments arranged by size: 5377 1382 705 277 62 37 27 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5998 6863 7161 5998 Size: 865 298 6710 Fragments arranged by size: 6710 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3898 6536 882 Size: 617 2399 2638 2219 Fragments arranged by size: 2638 2399 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5170 5664 493 Size: 711 3966 494 2702 Fragments arranged by size: 3966 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3105 5000 7867 3105 Size: 1895 2867 3111 Fragments arranged by size: 3111 2867 1895 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5139 5680 6062 6697 Size: 53 83 186 936 3606 541 382 635 Cuts at: 6697 275 Size: 1451 Fragments arranged by size: 3606 1451 936 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2719 2749 6306 6704 6978 7125 7536 2719 Size: 30 3557 398 274 147 411 3056 Fragments arranged by size: 3557 3056 411 398 274 147 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4007 4037 1178 Size: 30 165 30 2604 30 5014 Fragments arranged by size: 5014 2604 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2468 3076 4068 4414 4502 4558 4685 4862 Size: 1282 608 992 346 88 56 127 177 Cuts at: 4862 5091 1186 Size: 229 3968 Fragments arranged by size: 3968 1282 992 608 346 229 177 127 88 56 BseYI C'CCAG_C Cuts at: 2114 2579 2740 2823 2949 3401 3622 4743 7076 Size: 465 161 83 126 452 221 1121 2333 Cuts at: 7076 7572 2114 Size: 496 2415 Fragments arranged by size: 2415 2333 1121 496 465 452 221 161 126 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2384 3695 3887 1163 Size: 573 648 1311 192 5149 Fragments arranged by size: 5149 1311 648 573 192 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3134 5022 5323 6084 7112 1540 Size: 275 186 1133 1888 301 761 1028 2301 Fragments arranged by size: 2301 1888 1133 1028 761 301 275 186 BsiHKAI G_wGCw'C Cuts at: 2870 2963 3879 4776 5934 6019 7090 2870 Size: 93 916 897 1158 85 1071 3653 Fragments arranged by size: 3653 1158 1071 916 897 93 85 BsmI GAATG_Cn' Cuts at: 4402 4509 5384 5477 4402 Size: 107 875 93 6798 Fragments arranged by size: 6798 875 107 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2886 2886 Size: 7873 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2450 2529 2792 2870 2963 3187 3258 Size: 399 660 79 263 78 93 224 71 Cuts at: 3258 3393 3413 3427 3460 3879 4034 4139 4160 Size: 135 20 14 33 419 155 105 21 Cuts at: 4160 4197 4278 4338 4410 4743 4776 4844 4926 Size: 37 81 60 72 333 33 68 82 Cuts at: 4926 4956 5192 5934 6019 7090 1391 Size: 30 236 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 660 419 399 333 263 236 224 155 135 105 93 85 82 81 79 78 72 71 68 60 37 33 33 30 21 20 14 BspEI T'CCGG_A Cuts at: 7536 7536 Size: 7873 BspHI T'CATG_A Cuts at: 5762 7492 5762 Size: 1730 6143 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5228 844 Size: 4384 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2110 3378 3396 3447 3469 5760 1394 Size: 429 287 1268 18 51 22 2291 3507 Fragments arranged by size: 3507 2291 1268 429 287 51 22 18 BsrDI GCAATG_nn' Cuts at: 65 1248 3220 4238 4893 5063 6368 6542 65 Size: 1183 1972 1018 655 170 1305 174 1396 Fragments arranged by size: 1972 1396 1305 1183 1018 655 174 170 BsrFI r'CCGG_y Cuts at: 1700 1810 2856 2907 3154 3666 3957 6517 6704 Size: 110 1046 51 247 512 291 2560 187 Cuts at: 6704 1700 Size: 2869 Fragments arranged by size: 2869 2560 1046 512 291 247 187 110 51 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7873 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7873 BssSI C'ACGA_G Cuts at: 4628 5933 6945 4628 Size: 1305 1012 5556 Fragments arranged by size: 5556 1305 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7873 BstBI TT'CG_AA Cuts at: 2007 6677 2007 Size: 4670 3203 BstEII G'GTnAC_C Cuts at: 1184 4369 1184 Size: 3185 4688 BstXI CCAn_nnnn'nTGG Cuts at: 2831 2831 Size: 7873 BstYI r'GATC_y Cuts at: 1972 2469 2502 3291 3975 3984 5203 5570 5955 Size: 497 33 789 684 9 1219 367 385 Cuts at: 5955 5972 7424 7510 7522 7868 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1219 789 684 497 385 367 346 86 33 17 12 9 Bsu36I CC'TnA_GG Cuts at: 4845 4845 Size: 7873 BtgI C'CryG_G Cuts at: 513 1065 2478 3843 5132 513 Size: 552 1413 1365 1289 3254 Fragments arranged by size: 3254 1413 1365 1289 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2134 487 Size: 683 56 908 6226 Fragments arranged by size: 6226 908 683 56 BtsI GCAGTG_nn' Cuts at: 2255 2367 2987 3490 3794 4389 5389 6183 6203 Size: 112 620 503 304 595 1000 794 20 Cuts at: 6203 2255 Size: 3925 Fragments arranged by size: 3925 1000 794 620 595 503 304 112 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7873 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3202 3237 529 Size: 35 2638 35 5165 Fragments arranged by size: 5165 2638 35 35 DraI TTT'AAA Cuts at: 5177 5177 Size: 7873 DraIII CAC_nnn'GTG Cuts at: 3388 3455 3388 Size: 67 7806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6880 809 Size: 507 5564 1802 Fragments arranged by size: 5564 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2659 2858 3131 Size: 54 1425 50 275 18 829 199 273 Cuts at: 3131 4095 4560 4589 5320 6209 7838 8 Size: 964 465 29 731 889 1629 43 Fragments arranged by size: 1629 1425 964 889 829 731 465 275 273 199 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 3131 5320 1537 Size: 275 1319 2189 4090 Fragments arranged by size: 4090 2189 1319 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2225 2294 2307 5803 1353 Size: 872 69 13 3496 3423 Fragments arranged by size: 3496 3423 872 69 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2926 6460 6834 6980 387 Size: 1064 138 1337 3534 374 146 1280 Fragments arranged by size: 3534 1337 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2961 4774 2961 Size: 1813 6060 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2419 2493 2960 3036 3209 3758 Size: 393 456 331 74 467 76 173 549 Cuts at: 3758 4000 4670 4767 4841 5088 5936 6533 7320 Size: 242 670 97 74 247 848 597 787 Cuts at: 7320 7588 1239 Size: 268 1524 Fragments arranged by size: 1524 848 787 670 597 549 467 456 393 331 268 247 242 173 97 76 74 74 EcoNI CCTnn'n_nnAGG Cuts at: 5032 5032 Size: 7873 EcoO109I rG'GnC_Cy Cuts at: 2098 2788 3061 3184 3547 3723 4167 2098 Size: 690 273 123 363 176 444 5804 Fragments arranged by size: 5804 690 444 363 273 176 123 EcoRI G'AATT_C Cuts at: 5182 5560 5182 Size: 378 7495 FspI TGC'GCA Cuts at: 6379 6379 Size: 7873 HaeII r_GCGC'y Cuts at: 1678 2167 2263 3202 5142 7020 1678 Size: 489 96 939 1940 1878 2531 Fragments arranged by size: 2531 1940 1878 939 489 96 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2487 2519 3326 3358 6514 Size: 32 135 32 915 32 807 32 3156 Cuts at: 6514 6546 6588 6620 1373 Size: 32 42 32 2626 Fragments arranged by size: 3156 2626 915 807 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5217 5463 669 Size: 988 281 3279 246 3079 Fragments arranged by size: 3279 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7873 HpaI GTT'AAC Cuts at: 5463 5463 Size: 7873 KasI G'GCGC_C Cuts at: 5138 5138 Size: 7873 KpnI G_GTAC'C Cuts at: 3008 5198 3008 Size: 2190 5683 MfeI C'AATT_G Cuts at: 5472 5472 Size: 7873 MluI A'CGCG_T Cuts at: 7749 7749 Size: 7873 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4110 4212 4668 6987 7171 1305 Size: 2805 102 456 2319 184 2007 Fragments arranged by size: 2805 2319 2007 456 184 102 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6051 Fragments arranged by size: 6051 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 3821 4518 5831 6190 6349 518 Size: 648 1381 1274 697 1313 359 159 2042 Fragments arranged by size: 2042 1381 1313 1274 697 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2744 3238 4975 5289 5966 7114 7359 1606 Size: 1138 494 1737 314 677 1148 245 2120 Fragments arranged by size: 2120 1737 1148 1138 677 494 314 245 NaeI GCC'GGC Cuts at: 2858 3156 2858 Size: 298 7575 NarI GG'CG_CC Cuts at: 5139 5139 Size: 7873 NcoI C'CATG_G Cuts at: 513 1065 2478 513 Size: 552 1413 5908 Fragments arranged by size: 5908 1413 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7873 NgoMIV G'CCGG_C Cuts at: 2856 3154 2856 Size: 298 7575 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7873 NotI GC'GGCC_GC Cuts at: 5320 5320 Size: 7873 NspI r_CATG'y Cuts at: 1132 1229 2414 4688 5047 5231 6776 1132 Size: 97 1185 2274 359 184 1545 2229 Fragments arranged by size: 2274 2229 1545 1185 359 184 97 PasI CC'CwG_GG Cuts at: 1348 3180 1348 Size: 1832 6041 PciI A'CATG_T Cuts at: 6772 6772 Size: 7873 PflMI CCAn_nnn'nTGG Cuts at: 1108 2086 2479 2807 2835 3288 3736 1108 Size: 978 393 328 28 453 448 5245 Fragments arranged by size: 5245 978 453 448 393 328 28 PfoI T'CCnGG_A Cuts at: 3570 3987 3570 Size: 417 7456 PmeI GTTT'AAAC Cuts at: 5177 5177 Size: 7873 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3130 3162 4007 4039 5942 5974 7455 7487 3130 Size: 32 845 32 1903 32 1481 32 3516 Fragments arranged by size: 3516 1903 1481 845 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 3061 3547 3723 4167 2098 Size: 963 486 176 444 5804 Fragments arranged by size: 5804 963 486 444 176 PshAI GACnn'nnGTC Cuts at: 1613 4581 1613 Size: 2968 4905 PsiI TTA'TAA Cuts at: 5443 5443 Size: 7873 PspOMI G'GGCC_C Cuts at: 2788 3183 2788 Size: 395 7478 PspXI vC'TCGA_Gb Cuts at: 2493 2643 3881 2493 Size: 150 1238 6485 Fragments arranged by size: 6485 1238 150 PstI C_TGCA'G Cuts at: 830 3372 3801 4554 4980 5225 830 Size: 2542 429 753 426 245 3478 Fragments arranged by size: 3478 2542 753 429 426 245 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7873 PvuII CAG'CTG Cuts at: 2744 3238 4975 2744 Size: 494 1737 5642 Fragments arranged by size: 5642 1737 494 SacI G_AGCT'C Cuts at: 2963 4776 2963 Size: 1813 6060 SalI G'TCGA_C Cuts at: 1936 5215 1936 Size: 3279 4594 SanDI GG'GwC_CC Cuts at: 3061 3723 4167 3061 Size: 662 444 6767 Fragments arranged by size: 6767 662 444 SapI GCTCTTCn'nnn_ Cuts at: 2225 2225 Size: 7873 SbfI CC_TGCA'GG Cuts at: 5225 5225 Size: 7873 ScaI AGT'ACT Cuts at: 3071 3113 6121 7833 3071 Size: 42 3008 1712 3111 Fragments arranged by size: 3111 3008 1712 42 SfcI C'TryA_G Cuts at: 826 1045 3368 3415 3797 4078 4550 4976 5221 Size: 219 2323 47 382 281 472 426 245 Cuts at: 5221 6356 7037 7228 826 Size: 1135 681 191 1471 Fragments arranged by size: 2323 1471 1135 681 472 426 382 281 245 219 191 47 SfiI GGCCn_nnn'nGGCC Cuts at: 2695 4568 2695 Size: 1873 6000 SfoI GGC'GCC Cuts at: 5140 5140 Size: 7873 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7873 SgrAI Cr'CCGG_yG Cuts at: 1700 2907 1700 Size: 1207 6666 SmaI CCC'GGG Cuts at: 1872 4028 4703 5200 1872 Size: 2156 675 497 4545 Fragments arranged by size: 4545 2156 675 497 SmlI C'TyrA_G Cuts at: 820 1017 1943 2493 2643 3881 5977 6878 7140 Size: 197 926 550 150 1238 2096 901 262 Cuts at: 7140 820 Size: 1553 Fragments arranged by size: 2096 1553 1238 926 901 550 262 197 150 SnaBI TAC'GTA Cuts at: 493 5170 5664 493 Size: 4677 494 2702 Fragments arranged by size: 4677 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7873 SphI G_CATG'C Cuts at: 1132 2414 4688 5047 5231 1132 Size: 1282 2274 359 184 3774 Fragments arranged by size: 3774 2274 1282 359 184 SspI AAT'ATT Cuts at: 5 52 5797 5 Size: 47 5745 2081 Fragments arranged by size: 5745 2081 47 StuI AGG'CCT Cuts at: 1517 2465 2689 5027 1517 Size: 948 224 2338 4363 Fragments arranged by size: 4363 2338 948 224 StyI C'CwwG_G Cuts at: 513 1065 2205 2478 3543 4197 5312 513 Size: 552 1140 273 1065 654 1115 3074 Fragments arranged by size: 3074 1140 1115 1065 654 552 273 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2593 2974 3415 3466 3953 4515 5036 Size: 1255 1013 381 441 51 487 562 521 Cuts at: 5036 5900 5917 6070 6255 6700 325 Size: 864 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1013 864 562 521 487 445 441 381 185 153 51 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2497 3069 3111 Size: 274 80 33 51 1443 520 572 42 Cuts at: 3111 6119 7831 96 Size: 3008 1712 138 Fragments arranged by size: 3008 1712 1443 572 520 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4387 4419 7833 7865 4387 Size: 32 3414 32 4395 Fragments arranged by size: 4395 3414 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4790 1316 Size: 3474 4399 XbaI T'CTAG_A Cuts at: 5209 5209 Size: 7873 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2083 2083 Size: 7873 XhoI C'TCGA_G Cuts at: 1943 2493 2643 3881 1943 Size: 550 150 1238 5935 Fragments arranged by size: 5935 1238 550 150 XmaI C'CCGG_G Cuts at: 1870 4026 4701 5198 1870 Size: 2156 675 497 4545 Fragments arranged by size: 4545 2156 675 497 XmnI GAAnn'nnTTC Cuts at: 3021 6002 3021 Size: 2981 4892 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5681 276 Size: 53 83 186 936 4147 2468 Fragments arranged by size: 4147 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacII SexAI SrfI SwaI