Restriction map for
pFN21ASDA0964
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BamHI BbrPI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BseAI BsgI
BsiWI BsmI Bsp1407I BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
CelII CpoI DraI Eam1105I Eco47III
EcoO65I EcoRI EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MflI MluI MroI
MseI MunI NdeI NheI NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SalI SapI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI SwaI Tru9I XbaI XhoII
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 2557 AAATTC [GA]^AATT[CT]
AgeI 1686 ACCGGT A^CCGGT
AlwI 1377 GATCC GGATC(4/5)
AocI 1888 CCTGAGG CC^T[ACGT]AGG
ApaI 1068 GGGCCC GGGCC^C
ApoI 2557 AAATTC [GA]^AATT[CT]
AspI 1576 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1091 TGCGCA TGC^GCA
BalI 1392 TGGCCA TGG^CCA
BciVI 2139 GGATAC GTATCC(6/5)
BsrDI 497 GCAATG GCAATG(2/0)
Bsu36I 1888 CCTGAGG CC^T[ACGT]AGG
DraIII 1865 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1797 GACTAGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1061 CGGCCG C^GGCCG
EclXI 1061 CGGCCG C^GGCCG
Eco52I 1061 CGGCCG C^GGCCG
Eco81I 1888 CCTGAGG CC^T[ACGT]AGG
EcoNI 2037 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2671 GATATC GAT^ATC
FspI 1091 TGCGCA TGC^GCA
MaeII 863 ACGT A^CGT
MluNI 1392 TGGCCA TGG^CCA
MscI 1392 TGGCCA TGG^CCA
MslI 343 CACCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1115 CCATGG C^CATGG
PaeR7I 2341 CTCGAG C^TCGAG
PflFI 1576 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1686 ACCGGT A^CCGGT
PvuI 2063 CGATCG CGAT^CG
SacI 615 GAGCTC GAGCT^C
SbfI 993 CCTGCAGG CCTGCA^GG
ScaI 1741 AGTACT AGT^ACT
SexAI 422 ACCTGGT A^CC[AT]GGT
SmaI 139 CCCGGG CCC^GGG
SphI 1088 GCATGC GCATG^C
Sse8387I 993 CCTGCAGG CCTGCA^GG
TfiI 1258 GATTC G^A[AT]TC
Tth111I 1576 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2341 CTCGAG C^TCGAG
XmaCI 139 CCCGGG C^CCGGG
XmaI 139 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AspHI 615 GAGCTC G[AT]GC[AT]^C
1023 GAGCAC
BbeI 646 GGCGCC GGCGC^C
1181 GGCGCC
BbsI 921 GAAGAC GAAGAC(2/6)
1674 GAAGAC
BpuAI 921 GAAGAC GAAGAC(2/6)
1674 GAAGAC
BsaWI 1686 ACCGGT [AT]^CCGG[AT]
2772 TCCGGT
BsiHKAI 615 GAGCTC G[AT]GC[AT]^C
1023 GAGCAC
BssSI 58 CACGAG CACGAG(-5/-1)
907 CACGAG
KasI 646 GGCGCC G^GCGCC
1181 GGCGCC
KspI 1058 CCGCGG CCGC^GG
1172 CCGCGG
NarI 646 GGCGCC GG^CGCC
1181 GGCGCC
PpuMI 2627 AGGACCT [GA]G^G[AT]CC[CT]
2645 GGGACCT
SacII 1058 CCGCGG CCGC^GG
1172 CCGCGG
SfoI 646 GGCGCC GGC^GCC
1181 GGCGCC
Tsp509I 368 AATT ^AATT
2558 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI AvaII BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspDI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I ClaI
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI