Restriction map for
pFN21ASDA0964
(Linear) (Six-base) MAPSORT of: KIAA0964.seq Check: 3881 from: 1 to: 2967
KIAA0964.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 835 987 2967
Size: 835 152 1980
Fragments arranged by size:
1980 835 152
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 2516 2967
Size: 339 2177 451
Fragments arranged by size:
2177 451 339
AgeI A'CCGG_T
Cuts at: 0 1686 2967
Size: 1686 1281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1048 2967
Size: 1048 1919
AlwNI CAG_nnn'CTG
Cuts at: 0 673 1215 1888 2544 2631 2893 2967
Size: 673 542 673 656 87 262 74
Fragments arranged by size:
673 673 656 542 262 87 74
ApaI G_GGCC'C
Cuts at: 0 1072 2967
Size: 1072 1895
ApoI r'AATT_y
Cuts at: 0 2557 2967
Size: 2557 410
AvaI C'yCGr_G
Cuts at: 0 139 1966 2341 2737 2967
Size: 139 1827 375 396 230
Fragments arranged by size:
1827 396 375 230 139
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1827 1860 2967
Size: 1827 33 1107
Fragments arranged by size:
1827 1107 33
BanI G'GyrC_C
Cuts at: 0 243 459 646 999 1181 2076 2967
Size: 243 216 187 353 182 895 891
Fragments arranged by size:
895 891 353 243 216 187 182
BanII G_rGCy'C
Cuts at: 0 65 259 406 578 619 877 1072 2967
Size: 65 194 147 172 41 258 195 1895
Fragments arranged by size:
1895 258 195 194 172 147 65 41
BbeI G_GCGC'C
Cuts at: 0 650 1185 2967
Size: 650 535 1782
Fragments arranged by size:
1782 650 535
BbsI GAAGACnn'nnnn_
Cuts at: 0 928 1681 2967
Size: 928 753 1286
Fragments arranged by size:
1286 928 753
BbvCI CC'TCA_GC
Cuts at: 0 895 927 1624 2967
Size: 895 32 697 1343
Fragments arranged by size:
1343 895 697 32
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1036 1070 1544 1578 2667 2701 2967
Size: 1036 34 474 34 1089 34 266
Fragments arranged by size:
1089 1036 474 266 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2133 2967
Size: 2133 834
BglI GCCn_nnn'nGGC
Cuts at: 0 127 582 1552 1808 1891 2308 2399 2884
Size: 127 455 970 256 83 417 91 485
Cuts at: 2884 2967
Size: 83
Fragments arranged by size:
970 485 455 417 256 127 91 83
83
Bme1580I G_kGCm'C
Cuts at: 0 478 755 850 1004 1072 2538 2967
Size: 478 277 95 154 68 1466 429
Fragments arranged by size:
1466 478 429 277 154 95 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 1377 1604 2967
Size: 1377 227 1363
Fragments arranged by size:
1377 1363 227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 184 2331 2367 2967
Size: 184 2147 36 600
Fragments arranged by size:
2147 600 184 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1323 2337 2967
Size: 490 833 1014 630
Fragments arranged by size:
1014 833 630 490
Bpu10I CC'TnA_GC
Cuts at: 0 895 927 1624 1813 2967
Size: 895 32 697 189 1154
Fragments arranged by size:
1154 895 697 189 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 2145 2689 2967
Size: 937 1208 544 278
Fragments arranged by size:
1208 937 544 278
BsaI GGTCTCn'nnnn_
Cuts at: 0 2338 2467 2725 2967
Size: 2338 129 258 242
Fragments arranged by size:
2338 258 242 129
BsaHI Gr'CG_yC
Cuts at: 0 647 1182 2311 2831 2967
Size: 647 535 1129 520 136
Fragments arranged by size:
1129 647 535 520 136
BsaWI w'CCGG_w
Cuts at: 0 1686 2772 2967
Size: 1686 1086 195
Fragments arranged by size:
1686 1086 195
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 214 244 1504 1534 1567 1597 2197 2227
Size: 214 30 1260 30 33 30 600 30
Cuts at: 2227 2967
Size: 740
Fragments arranged by size:
1260 740 600 214 33 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 1585 2073 2196 2340 2967
Size: 265 1320 488 123 144 627
Fragments arranged by size:
1320 627 488 265 144 123
BseYI C'CCAG_C
Cuts at: 0 199 265 568 666 819 832 1215 1295
Size: 199 66 303 98 153 13 383 80
Cuts at: 1295 1507 1855 1881 2361 2537 2967
Size: 212 348 26 480 176 430
Fragments arranged by size:
480 430 383 348 303 212 199 176
153 98 80 66 26 13
BsiEI CG_ry'CG
Cuts at: 0 84 1064 1852 2066 2967
Size: 84 980 788 214 901
Fragments arranged by size:
980 901 788 214 84
BsiHKAI G_wGCw'C
Cuts at: 0 619 1027 2967
Size: 619 408 1940
Fragments arranged by size:
1940 619 408
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1042 1480 2390 2967
Size: 1042 438 910 577
Fragments arranged by size:
1042 910 577 438
Bsp1286I G_dGCh'C
Cuts at: 0 65 259 406 478 578 619 755 850
Size: 65 194 147 72 100 41 136 95
Cuts at: 850 877 1004 1027 1072 2538 2967
Size: 27 127 23 45 1466 429
Fragments arranged by size:
1466 429 194 147 136 127 100 95
72 65 45 41 27 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 835 987 1001 1395 1547 2657 2967
Size: 104 731 152 14 394 152 1110 310
Fragments arranged by size:
1110 731 394 310 152 152 104 14
BsrBI CCG'CTC
Cuts at: 0 558 593 1520 2439 2967
Size: 558 35 927 919 528
Fragments arranged by size:
927 919 558 528 35
BsrDI GCAATG_nn'
Cuts at: 0 504 2967
Size: 504 2463
BsrFI r'CCGG_y
Cuts at: 0 293 643 1082 1615 1686 2230 2370 2802
Size: 293 350 439 533 71 544 140 432
Cuts at: 2802 2967
Size: 165
Fragments arranged by size:
544 533 439 432 350 293 165 140
71
BssSI C'ACGA_G
Cuts at: 0 58 907 2967
Size: 58 849 2060
Fragments arranged by size:
2060 849 58
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 349 1360 1390 2967
Size: 312 37 1011 30 1577
Fragments arranged by size:
1577 1011 312 37 30
Bsu36I CC'TnA_GG
Cuts at: 0 1889 2967
Size: 1889 1078
BtgI C'CryG_G
Cuts at: 0 107 375 393 1058 1115 1172 2967
Size: 107 268 18 665 57 57 1795
Fragments arranged by size:
1795 665 268 107 57 57 18
BtsI GCAGTG_nn'
Cuts at: 0 666 1467 1501 2538 2813 2967
Size: 666 801 34 1037 275 154
Fragments arranged by size:
1037 801 666 275 154 34
ClaI AT'CG_AT
Cuts at: 0 1996 2066 2225 2967
Size: 1996 70 159 742
Fragments arranged by size:
1996 742 159 70
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 375 410 474 495 509 530 2967
Size: 375 35 64 21 14 21 2437
Fragments arranged by size:
2437 375 64 35 21 21 14
DraIII CAC_nnn'GTG
Cuts at: 0 1870 2967
Size: 1870 1097
DrdI GACnn_nn'nnGTC
Cuts at: 0 1803 2967
Size: 1803 1164
EaeI y'GGCC_r
Cuts at: 0 408 1061 1392 2876 2967
Size: 408 653 331 1484 91
Fragments arranged by size:
1484 653 408 331 91
EagI C'GGCC_G
Cuts at: 0 1061 2967
Size: 1061 1906
EarI CTCTTCn'nnn_
Cuts at: 0 1295 2737 2747 2967
Size: 1295 1442 10 220
Fragments arranged by size:
1442 1295 220 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 406 2967
Size: 406 2561
EcoICRI GAG'CTC
Cuts at: 0 617 2967
Size: 617 2350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 490 1323 2337 2516 2967
Size: 339 151 833 1014 179 451
Fragments arranged by size:
1014 833 451 339 179 151
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2041 2967
Size: 2041 926
EcoO109I rG'GnC_Cy
Cuts at: 0 1068 1069 1893 2244 2384 2574 2628 2646
Size: 1068 1 824 351 140 190 54 18
Cuts at: 2646 2967
Size: 321
Fragments arranged by size:
1068 824 351 321 190 140 54 18
1
EcoRV GAT'ATC
Cuts at: 0 2673 2967
Size: 2673 294
FspI TGC'GCA
Cuts at: 0 1093 2967
Size: 1093 1874
HaeII r_GCGC'y
Cuts at: 0 650 1185 2850 2967
Size: 650 535 1665 117
Fragments arranged by size:
1665 650 535 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1567 1599 2119 2151 2809 2841 2967
Size: 1567 32 520 32 658 32 126
Fragments arranged by size:
1567 658 520 126 32 32 32
KasI G'GCGC_C
Cuts at: 0 646 1181 2967
Size: 646 535 1786
Fragments arranged by size:
1786 646 535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 620 1805 2967
Size: 62 558 1185 1162
Fragments arranged by size:
1185 1162 558 62
MscI TGG'CCA
Cuts at: 0 1394 2967
Size: 1394 1573
MslI CAynn'nnrTG
Cuts at: 0 347 2967
Size: 347 2620
MspA1I CmG'CkG
Cuts at: 0 189 333 441 481 1060 1097 1174 1343
Size: 189 144 108 40 579 37 77 169
Cuts at: 1343 1480 2658 2717 2724 2967
Size: 137 1178 59 7 243
Fragments arranged by size:
1178 579 243 189 169 144 137 108
77 59 40 37 7
NaeI GCC'GGC
Cuts at: 0 645 1084 2372 2804 2967
Size: 645 439 1288 432 163
Fragments arranged by size:
1288 645 439 432 163
NarI GG'CG_CC
Cuts at: 0 647 1182 2967
Size: 647 535 1785
Fragments arranged by size:
1785 647 535
NcoI C'CATG_G
Cuts at: 0 1115 2967
Size: 1115 1852
NgoMIV G'CCGG_C
Cuts at: 0 643 1082 2370 2802 2967
Size: 643 439 1288 432 165
Fragments arranged by size:
1288 643 439 432 165
NspI r_CATG'y
Cuts at: 0 759 1092 1272 1918 2967
Size: 759 333 180 646 1049
Fragments arranged by size:
1049 759 646 333 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 163 673 2631 2717 2967
Size: 163 510 1958 86 250
Fragments arranged by size:
1958 510 250 163 86
PfoI T'CCnGG_A
Cuts at: 0 311 2189 2945 2967
Size: 311 1878 756 22
Fragments arranged by size:
1878 756 311 22
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2476 2508 2967
Size: 2476 32 459
Fragments arranged by size:
2476 459 32
PpuMI rG'GwC_Cy
Cuts at: 0 2628 2646 2967
Size: 2628 18 321
Fragments arranged by size:
2628 321 18
PspOMI G'GGCC_C
Cuts at: 0 1068 2967
Size: 1068 1899
PstI C_TGCA'G
Cuts at: 0 998 1462 1592 2426 2967
Size: 998 464 130 834 541
Fragments arranged by size:
998 834 541 464 130
PvuI CG_AT'CG
Cuts at: 0 2066 2967
Size: 2066 901
PvuII CAG'CTG
Cuts at: 0 189 333 1343 2658 2717 2724 2967
Size: 189 144 1010 1315 59 7 243
Fragments arranged by size:
1315 1010 243 189 144 59 7
SacI G_AGCT'C
Cuts at: 0 619 2967
Size: 619 2348
SacII CC_GC'GG
Cuts at: 0 1061 1175 2967
Size: 1061 114 1792
Fragments arranged by size:
1792 1061 114
SbfI CC_TGCA'GG
Cuts at: 0 998 2967
Size: 998 1969
ScaI AGT'ACT
Cuts at: 0 1743 2967
Size: 1743 1224
SexAI A'CCwGG_T
Cuts at: 0 422 2967
Size: 422 2545
SfcI C'TryA_G
Cuts at: 0 994 1458 1588 2422 2653 2967
Size: 994 464 130 834 231 314
Fragments arranged by size:
994 834 464 314 231 130
SfoI GGC'GCC
Cuts at: 0 648 1183 2967
Size: 648 535 1784
Fragments arranged by size:
1784 648 535
SmaI CCC'GGG
Cuts at: 0 141 2967
Size: 141 2826
SmlI C'TyrA_G
Cuts at: 0 952 2160 2341 2704 2967
Size: 952 1208 181 363 263
Fragments arranged by size:
1208 952 363 263 181
SphI G_CATG'C
Cuts at: 0 1092 2967
Size: 1092 1875
StuI AGG'CCT
Cuts at: 0 8 115 1369 2824 2967
Size: 8 107 1254 1455 143
Fragments arranged by size:
1455 1254 143 107 8
StyI C'CwwG_G
Cuts at: 0 386 455 563 837 935 1115 2967
Size: 386 69 108 274 98 180 1852
Fragments arranged by size:
1852 386 274 180 108 98 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 94 1179 2967
Size: 94 1085 1788
Fragments arranged by size:
1788 1085 94
TatI w'GTAC_w
Cuts at: 0 1741 2967
Size: 1741 1226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 214 246 2098 2130 2197 2229 2437 2469
Size: 214 32 1852 32 67 32 208 32
Cuts at: 2469 2967
Size: 498
Fragments arranged by size:
1852 498 214 208 67 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1579 2967
Size: 1579 1388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 373 840 2333 2967
Size: 373 467 1493 634
Fragments arranged by size:
1493 634 467 373
XhoI C'TCGA_G
Cuts at: 0 2341 2967
Size: 2341 626
XmaI C'CCGG_G
Cuts at: 0 139 2967
Size: 139 2828
Enzymes that do cut:
AarI AcuI AgeI AlfI AlwNI ApaI ApoI AvaI
BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstXI Bsu36I
BtgI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PflMI PfoI PpiI PpuMI
PspOMI PstI PvuI PvuII SacI SacII SbfI ScaI
SexAI SfcI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BamHI
BclI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI
BsgI BsiWI BsmI BspEI BspHI BsrGI BssHII BstAPI
BstBI BstEII BstYI BstZ17I BtgZI DraI EcoRI FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PshAI PsiI PspXI PsrI RsrII SalI
SanDI SapI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XbaI XmnI ZraI