(Circular) (Six-base) MAPSORT of: pFN21ASDA0961.seq Check: 7313 from: 1 to: 6286 pFN21ASDA0961 6286 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2061 2629 3629 1937 Size: 124 568 1000 4594 Fragments arranged by size: 4594 1000 568 124 Acc65I G'GTAC_C Cuts at: 3607 3607 Size: 6286 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4349 5733 6001 1632 Size: 2717 1384 268 1917 Fragments arranged by size: 2717 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6089 AflIII A'CryG_T Cuts at: 1203 2693 5185 6162 1203 Size: 1490 2492 977 1327 Fragments arranged by size: 2492 1490 1327 977 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 3111 5015 3111 Size: 1904 4382 AleI CACnn'nnGTG Cuts at: 1166 2817 3069 1166 Size: 1651 252 4383 Fragments arranged by size: 4383 1651 252 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6286 AlwNI CAG_nnn'CTG Cuts at: 2893 4029 5601 2893 Size: 1136 1572 3578 Fragments arranged by size: 3578 1572 1136 ApaLI G'TGCA_C Cuts at: 4343 5499 4343 Size: 1156 5130 ApoI r'AATT_y Cuts at: 1493 2107 2556 2640 2724 3060 3480 3564 3595 Size: 614 449 84 84 336 420 84 31 Cuts at: 3595 3821 3973 6082 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 614 449 420 336 226 152 84 84 84 31 AseI AT'TA_AT Cuts at: 160 1032 4840 160 Size: 872 3808 1606 Fragments arranged by size: 3808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 3611 1870 Size: 73 1668 4545 Fragments arranged by size: 4545 1668 73 BamHI G'GATC_C Cuts at: 3616 3983 3616 Size: 367 5919 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3607 5062 618 Size: 325 217 627 1820 1455 1842 Fragments arranged by size: 1842 1820 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2217 3605 1391 Size: 826 1388 4072 Fragments arranged by size: 4072 1388 826 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3110 3362 928 Size: 971 1211 252 3852 Fragments arranged by size: 3852 1211 971 252 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4477 4511 1903 Size: 34 2540 34 3678 Fragments arranged by size: 3678 2540 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 2187 1548 Size: 639 5647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4897 136 Size: 107 122 71 631 605 3225 1525 Fragments arranged by size: 3225 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2685 6281 2685 Size: 3596 2690 BlpI GC'TnA_GC Cuts at: 3703 3703 Size: 6286 Bme1580I G_kGCm'C Cuts at: 1790 2620 4347 5503 1790 Size: 830 1727 1156 2573 Fragments arranged by size: 2573 1727 1156 830 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4017 4975 442 Size: 416 53 83 399 24 2600 958 1753 Fragments arranged by size: 2600 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3006 4946 1239 Size: 1767 1940 2579 Fragments arranged by size: 2579 1940 1767 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4411 5276 5574 4411 Size: 865 298 5123 Fragments arranged by size: 5123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4949 882 Size: 617 3450 2219 Fragments arranged by size: 3450 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3583 4077 493 Size: 711 2379 494 2702 Fragments arranged by size: 2702 2379 711 494 BsaBI GATnn'nnATC Cuts at: 6280 6280 Size: 6286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4093 4475 5110 275 Size: 53 83 186 936 2560 382 635 1451 Fragments arranged by size: 2560 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2781 2811 3033 3063 1178 Size: 30 165 30 1378 30 222 30 4401 Fragments arranged by size: 4401 1378 222 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6286 BseYI C'CCAG_C Cuts at: 5489 5985 5489 Size: 496 5790 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5713 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4347 4432 5503 4347 Size: 85 1071 5130 Fragments arranged by size: 5130 1071 85 BsmI GAATG_Cn' Cuts at: 2090 3797 3890 2090 Size: 1707 93 4486 Fragments arranged by size: 4486 1707 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2620 3605 4347 4432 5503 1391 Size: 399 427 403 985 742 85 1071 2174 Fragments arranged by size: 2174 1071 985 742 427 403 399 85 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 2383 4175 5905 2383 Size: 1792 1730 2764 Fragments arranged by size: 2764 1792 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3641 844 Size: 2797 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4173 1394 Size: 429 2350 3507 Fragments arranged by size: 3507 2350 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2898 4781 4955 65 Size: 1183 1650 1883 174 1396 Fragments arranged by size: 1883 1650 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4930 5117 1700 Size: 110 3120 187 2869 Fragments arranged by size: 3120 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2112 96 Size: 2016 4270 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 2028 4346 5358 2028 Size: 2318 1012 2956 Fragments arranged by size: 2956 2318 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6286 BstBI TT'CG_AA Cuts at: 2007 5090 2007 Size: 3083 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2685 3616 3983 4368 4385 5837 5923 5935 Size: 713 931 367 385 17 1452 86 12 Cuts at: 5935 6281 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 931 713 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4777 Fragments arranged by size: 4777 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5547 Fragments arranged by size: 5547 683 56 BtsI GCAGTG_nn' Cuts at: 3802 4596 4616 3802 Size: 794 20 5472 Fragments arranged by size: 5472 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251 Size: 54 1425 50 275 18 1903 889 1629 Cuts at: 6251 8 Size: 43 Fragments arranged by size: 1903 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2460 4216 1353 Size: 1107 1756 3423 Fragments arranged by size: 3423 1756 1107 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3006 4349 4946 5733 6001 1239 Size: 393 1374 1343 597 787 268 1524 Fragments arranged by size: 1524 1374 1343 787 597 393 268 EcoRI G'AATT_C Cuts at: 2556 2640 2724 3060 3480 3564 3595 3973 2556 Size: 84 84 336 420 84 31 378 4869 Fragments arranged by size: 4869 420 378 336 84 84 84 31 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 5433 1678 Size: 3755 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 3097 3129 3457 Size: 32 135 32 677 32 816 32 328 Cuts at: 3457 3489 4927 4959 5001 5033 1373 Size: 32 1438 32 42 32 2626 Fragments arranged by size: 2626 1438 816 677 328 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2630 3630 3876 669 Size: 988 281 692 1000 246 3079 Fragments arranged by size: 3079 1000 988 692 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6286 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 3611 3611 Size: 6286 MfeI C'AATT_G Cuts at: 3885 3885 Size: 6286 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5400 5584 1305 Size: 4095 184 2007 Fragments arranged by size: 4095 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4464 Fragments arranged by size: 4464 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2565 2733 2817 3069 3153 3321 3405 Size: 648 1399 168 84 252 84 168 84 Cuts at: 3405 3489 3505 4244 4603 4762 518 Size: 84 16 739 359 159 2042 Fragments arranged by size: 2042 1399 739 648 359 252 168 168 159 84 84 84 84 16 MspA1I CmG'CkG Cuts at: 1606 3702 4379 5527 5772 1606 Size: 2096 677 1148 245 2120 Fragments arranged by size: 2120 2096 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4777 Fragments arranged by size: 4777 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 2697 3644 5189 1132 Size: 97 1468 947 1545 2229 Fragments arranged by size: 2229 1545 1468 947 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 2693 5185 2693 Size: 2492 3794 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6286 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2043 4355 4387 5868 5900 2011 Size: 32 2312 32 1481 32 2397 Fragments arranged by size: 2397 2312 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6286 PsiI TTA'TAA Cuts at: 3856 3856 Size: 6286 PstI C_TGCA'G Cuts at: 830 3638 830 Size: 2808 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 SalI G'TCGA_C Cuts at: 1936 2628 3628 1936 Size: 692 1000 4594 Fragments arranged by size: 4594 1000 692 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 4534 6246 4534 Size: 1712 4574 SfcI C'TryA_G Cuts at: 826 1045 2058 2137 3634 4769 5450 5641 826 Size: 219 1013 79 1497 1135 681 191 1471 Fragments arranged by size: 1497 1471 1135 1013 681 219 191 79 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4390 5291 5553 820 Size: 197 926 2447 901 262 1553 Fragments arranged by size: 2447 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3583 4077 493 Size: 3090 494 2702 Fragments arranged by size: 3090 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 3644 1132 Size: 2512 3774 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 2855 2939 2958 3023 3443 1517 Size: 1338 84 19 65 420 4360 Fragments arranged by size: 4360 1338 420 84 65 19 StyI C'CwwG_G Cuts at: 513 1065 2022 3725 513 Size: 552 957 1703 3074 Fragments arranged by size: 3074 1703 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4313 4330 4483 4668 5113 325 Size: 1255 2733 17 153 185 445 1498 Fragments arranged by size: 2733 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 2713 2951 Size: 274 80 33 51 1443 135 601 238 Cuts at: 2951 4532 6244 96 Size: 1581 1712 138 Fragments arranged by size: 1712 1581 1443 601 274 238 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2440 2472 6246 6278 2440 Size: 32 3774 32 2448 Fragments arranged by size: 3774 2448 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6286 XbaI T'CTAG_A Cuts at: 2254 3622 2254 Size: 1368 4918 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 3107 3484 4415 3107 Size: 377 931 4978 Fragments arranged by size: 4978 931 377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI