(Circular) (Six-base) MAPSORT of: pFN21ASDA0957.seq Check: 1563 from: 1 to: 6805 pFN21ASDA0957 6805 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4615 278 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4148 1937 Size: 2211 4594 Acc65I G'GTAC_C Cuts at: 4126 4126 Size: 6805 AclI AA'CG_TT Cuts at: 1563 4932 5305 1563 Size: 3369 373 3063 Fragments arranged by size: 3369 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2228 4868 6252 6520 1632 Size: 596 2640 1384 268 1917 Fragments arranged by size: 2640 1917 1384 596 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6608 AflIII A'CryG_T Cuts at: 1203 5704 6681 1203 Size: 4501 977 1327 Fragments arranged by size: 4501 1327 977 AgeI A'CCGG_T Cuts at: 5636 5636 Size: 6805 AhdI GACnn_n'nnGTC Cuts at: 3555 5534 3555 Size: 1979 4826 AleI CACnn'nnGTG Cuts at: 1166 2273 1166 Size: 1107 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2184 2580 1184 Size: 1000 396 5409 Fragments arranged by size: 5409 1000 396 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2465 2497 2465 Size: 32 6773 AlwNI CAG_nnn'CTG Cuts at: 3454 3649 3840 4548 6120 3454 Size: 195 191 708 1572 4139 Fragments arranged by size: 4139 1572 708 195 191 ApaI G_GGCC'C Cuts at: 3753 3753 Size: 6805 ApaLI G'TGCA_C Cuts at: 4862 6018 4862 Size: 1156 5649 ApoI r'AATT_y Cuts at: 1493 4114 4340 4492 6601 1493 Size: 2621 226 152 2109 1697 Fragments arranged by size: 2621 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5359 160 Size: 872 4327 1606 Fragments arranged by size: 4327 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 AvaI C'yCGr_G Cuts at: 1870 1943 4130 1870 Size: 73 2187 4545 Fragments arranged by size: 4545 2187 73 BamHI G'GATC_C Cuts at: 4135 4502 4135 Size: 367 6438 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 3136 4126 5581 618 Size: 325 217 627 333 1016 990 1455 1842 Fragments arranged by size: 1842 1455 1016 990 627 333 325 217 BanII G_rGCy'C Cuts at: 1391 3753 4124 1391 Size: 2362 371 4072 Fragments arranged by size: 4072 2362 371 BbeI G_GCGC'C Cuts at: 2124 2124 Size: 6805 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3648 928 Size: 971 1749 4085 Fragments arranged by size: 4085 1749 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2300 2334 4996 5030 1903 Size: 34 363 34 2662 34 3678 Fragments arranged by size: 3678 2662 363 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4697 5907 4697 Size: 1210 5595 BclI T'GATC_A Cuts at: 1548 3736 1548 Size: 2188 4617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3805 5416 136 Size: 107 122 71 631 605 2133 1611 1525 Fragments arranged by size: 2133 1611 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2199 3731 6800 2199 Size: 1532 3069 2204 Fragments arranged by size: 3069 2204 1532 BlpI GC'TnA_GC Cuts at: 4222 4222 Size: 6805 Bme1580I G_kGCm'C Cuts at: 1790 2323 3753 3817 4866 6022 1790 Size: 533 1430 64 1049 1156 2573 Fragments arranged by size: 2573 1430 1156 1049 533 64 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6805 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3916 4536 5494 Size: 416 53 83 399 24 2499 620 958 Cuts at: 5494 442 Size: 1753 Fragments arranged by size: 2499 1753 958 620 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6805 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2661 2774 3200 3476 3492 3570 3623 3932 Size: 1422 113 426 276 16 78 53 309 Cuts at: 3932 5465 1239 Size: 1533 2579 Fragments arranged by size: 2579 1533 1422 426 309 276 113 78 53 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4930 5795 6093 4930 Size: 865 298 5642 Fragments arranged by size: 5642 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5468 882 Size: 617 3969 2219 Fragments arranged by size: 3969 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4102 4596 493 Size: 711 2898 494 2702 Fragments arranged by size: 2898 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6799 6799 Size: 6805 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 4612 4994 5629 Size: 53 83 186 936 588 2491 382 635 Cuts at: 5629 275 Size: 1451 Fragments arranged by size: 2491 1451 936 635 588 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5238 5636 5910 6057 6468 5238 Size: 398 274 147 411 5575 Fragments arranged by size: 5575 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2406 2436 2465 2495 2791 Size: 30 165 30 1003 30 29 30 296 Cuts at: 2791 2821 3173 3203 3638 3668 1178 Size: 30 352 30 435 30 4315 Fragments arranged by size: 4315 1003 435 352 296 165 30 30 30 30 30 30 30 29 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2588 2591 2944 3221 1186 Size: 1402 3 353 277 4770 Fragments arranged by size: 4770 1402 353 277 3 BseYI C'CCAG_C Cuts at: 2840 3223 3256 3279 3746 3816 4011 6008 6504 Size: 383 33 23 467 70 195 1997 496 Cuts at: 6504 2840 Size: 3141 Fragments arranged by size: 3141 1997 496 467 383 195 70 33 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2031 2441 4106 1163 Size: 573 295 410 1665 3862 Fragments arranged by size: 3862 1665 573 410 295 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2043 4255 5016 6044 1540 Size: 275 186 42 2212 761 1028 2301 Fragments arranged by size: 2301 2212 1028 761 275 186 42 BsiHKAI G_wGCw'C Cuts at: 2492 4866 4951 6022 2492 Size: 2374 85 1071 3275 Fragments arranged by size: 3275 2374 1071 85 BsmI GAATG_Cn' Cuts at: 4316 4409 4316 Size: 93 6712 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2323 2492 3753 3817 4124 4866 4951 Size: 399 533 169 1261 64 307 742 85 Cuts at: 4951 6022 1391 Size: 1071 2174 Fragments arranged by size: 2174 1261 1071 742 533 399 307 169 85 64 BspEI T'CCGG_A Cuts at: 6468 6468 Size: 6805 BspHI T'CATG_A Cuts at: 4694 6424 4694 Size: 1730 5075 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3018 3545 4160 844 Size: 2174 527 615 3489 Fragments arranged by size: 3489 2174 615 527 BsrBI CCG'CTC Cuts at: 1394 1823 4692 1394 Size: 429 2869 3507 Fragments arranged by size: 3507 2869 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5300 5474 65 Size: 1183 4052 174 1396 Fragments arranged by size: 4052 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5449 5636 1700 Size: 110 3639 187 2869 Fragments arranged by size: 3639 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3665 96 Size: 3569 3236 BssHII G'CGCG_C Cuts at: 1925 2567 1925 Size: 642 6163 BssSI C'ACGA_G Cuts at: 4865 5877 4865 Size: 1012 5793 BstAPI GCAn_nnn'nTGC Cuts at: 794 3289 794 Size: 2495 4310 BstBI TT'CG_AA Cuts at: 2007 5609 2007 Size: 3602 3203 BstEII G'GTnAC_C Cuts at: 1184 2134 1184 Size: 950 5855 BstXI CCAn_nnnn'nTGG Cuts at: 2240 2275 2993 2240 Size: 35 718 6052 Fragments arranged by size: 6052 718 35 BstYI r'GATC_y Cuts at: 1972 2199 3731 4135 4502 4887 4904 6356 6442 Size: 227 1532 404 367 385 17 1452 86 Cuts at: 6442 6454 6800 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1532 1452 404 385 367 346 227 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6253 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2278 487 Size: 683 56 1052 5014 Fragments arranged by size: 5014 1052 683 56 BtsI GCAGTG_nn' Cuts at: 2604 2824 2851 3621 4321 5115 5135 2604 Size: 220 27 770 700 794 20 4274 Fragments arranged by size: 4274 794 770 700 220 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6805 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6770 DraI TTT'AAA Cuts at: 4109 4109 Size: 6805 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2228 5812 809 Size: 507 912 3584 1802 Fragments arranged by size: 3584 1802 912 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2146 2508 2688 Size: 54 1425 50 275 18 316 362 180 Cuts at: 2688 2859 4252 5141 6770 8 Size: 171 1393 889 1629 43 Fragments arranged by size: 1629 1425 1393 889 362 316 275 180 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4252 1537 Size: 275 2440 4090 Fragments arranged by size: 4090 2440 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2796 2877 3785 4050 4735 1353 Size: 1443 81 908 265 685 3423 Fragments arranged by size: 3423 1443 908 685 265 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5392 5766 5912 387 Size: 1064 138 3803 374 146 1280 Fragments arranged by size: 3803 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2661 2774 3200 3476 3492 3570 Size: 393 596 433 113 426 276 16 78 Cuts at: 3570 3623 3932 4868 5465 6252 6520 1239 Size: 53 309 936 597 787 268 1524 Fragments arranged by size: 1524 936 787 597 596 433 426 393 309 276 268 113 78 53 16 EcoO109I rG'GnC_Cy Cuts at: 2514 3483 3610 3750 2514 Size: 969 127 140 5569 Fragments arranged by size: 5569 969 140 127 EcoRI G'AATT_C Cuts at: 4114 4492 4114 Size: 378 6427 EcoRV GAT'ATC Cuts at: 3405 3405 Size: 6805 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2807 4079 2807 Size: 1272 5533 FspI TGC'GCA Cuts at: 5311 5311 Size: 6805 HaeII r_GCGC'y Cuts at: 1678 2062 2124 2303 5952 1678 Size: 384 62 179 3649 2531 Fragments arranged by size: 3649 2531 384 179 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2876 2908 3212 3244 3436 Size: 32 135 32 1304 32 304 32 192 Cuts at: 3436 3468 3541 3573 3638 3670 3868 3900 5446 Size: 32 73 32 65 32 198 32 1546 Cuts at: 5446 5478 5520 5552 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1546 1304 304 198 192 135 73 65 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2731 3760 4149 4395 669 Size: 988 281 793 1029 389 246 3079 Fragments arranged by size: 3079 1029 988 793 389 281 246 HindIII A'AGCT_T Cuts at: 748 3464 748 Size: 2716 4089 HpaI GTT'AAC Cuts at: 2731 4395 2731 Size: 1664 5141 KasI G'GCGC_C Cuts at: 2120 2120 Size: 6805 KpnI G_GTAC'C Cuts at: 4130 4130 Size: 6805 MfeI C'AATT_G Cuts at: 4404 4404 Size: 6805 MluI A'CGCG_T Cuts at: 6681 6681 Size: 6805 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3171 3597 5919 6103 1305 Size: 1866 426 2322 184 2007 Fragments arranged by size: 2322 2007 1866 426 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2690 2861 10 Size: 54 1425 343 858 171 3954 Fragments arranged by size: 3954 1425 858 343 171 54 MslI CAynn'nnrTG Cuts at: 518 1166 2273 4763 5122 5281 518 Size: 648 1107 2490 359 159 2042 Fragments arranged by size: 2490 2042 1107 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2752 4221 4898 6046 6291 1606 Size: 1146 1469 677 1148 245 2120 Fragments arranged by size: 2120 1469 1148 1146 677 245 NarI GG'CG_CC Cuts at: 2121 2121 Size: 6805 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6253 NdeI CA'TA_TG Cuts at: 387 387 Size: 6805 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6805 NotI GC'GGCC_GC Cuts at: 4252 4252 Size: 6805 NspI r_CATG'y Cuts at: 1132 1229 3017 3439 4163 5708 1132 Size: 97 1788 422 724 1545 2229 Fragments arranged by size: 2229 1788 1545 724 422 97 PasI CC'CwG_GG Cuts at: 1348 2812 1348 Size: 1464 5341 PciI A'CATG_T Cuts at: 5704 5704 Size: 6805 PflMI CCAn_nnn'nTGG Cuts at: 1108 3146 3544 3910 1108 Size: 2038 398 366 4003 Fragments arranged by size: 4003 2038 398 366 PfoI T'CCnGG_A Cuts at: 2232 2232 Size: 6805 PmeI GTTT'AAAC Cuts at: 4109 4109 Size: 6805 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2465 2497 4874 4906 6387 6419 2465 Size: 32 2377 32 1481 32 2851 Fragments arranged by size: 2851 2377 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2514 2514 Size: 6805 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6805 PsiI TTA'TAA Cuts at: 4375 4375 Size: 6805 PspOMI G'GGCC_C Cuts at: 3749 3749 Size: 6805 PstI C_TGCA'G Cuts at: 830 3331 3491 3556 4157 830 Size: 2501 160 65 601 3478 Fragments arranged by size: 3478 2501 601 160 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6805 SalI G'TCGA_C Cuts at: 1936 4147 1936 Size: 2211 4594 SanDI GG'GwC_CC Cuts at: 2514 2514 Size: 6805 SapI GCTCTTCn'nnn_ Cuts at: 2796 2796 Size: 6805 SbfI CC_TGCA'GG Cuts at: 4157 4157 Size: 6805 ScaI AGT'ACT Cuts at: 5053 6765 5053 Size: 1712 5093 SexAI A'CCwGG_T Cuts at: 3428 3428 Size: 6805 SfcI C'TryA_G Cuts at: 826 1045 3327 3487 3525 3552 4153 5288 5969 Size: 219 2282 160 38 27 601 1135 681 Cuts at: 5969 6160 826 Size: 191 1471 Fragments arranged by size: 2282 1471 1135 681 601 219 191 160 38 27 SfoI GGC'GCC Cuts at: 2122 2122 Size: 6805 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6805 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6805 SmaI CCC'GGG Cuts at: 1872 4132 1872 Size: 2260 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4909 5810 6072 820 Size: 197 926 2966 901 262 1553 Fragments arranged by size: 2966 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4102 4596 493 Size: 3609 494 2702 Fragments arranged by size: 3609 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6805 SphI G_CATG'C Cuts at: 1132 3017 4163 1132 Size: 1885 1146 3774 Fragments arranged by size: 3774 1885 1146 SspI AAT'ATT Cuts at: 5 52 4729 5 Size: 47 4677 2081 Fragments arranged by size: 4677 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6805 StyI C'CwwG_G Cuts at: 513 1065 2946 3408 4244 513 Size: 552 1881 462 836 3074 Fragments arranged by size: 3074 1881 836 552 462 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4832 4849 5002 5187 5632 325 Size: 1255 3252 17 153 185 445 1498 Fragments arranged by size: 3252 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3665 5051 6763 Size: 274 80 33 51 1443 1688 1386 1712 Cuts at: 6763 96 Size: 138 Fragments arranged by size: 1712 1688 1443 1386 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2406 2438 6765 6797 2406 Size: 32 4327 32 2414 Fragments arranged by size: 4327 2414 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6805 XbaI T'CTAG_A Cuts at: 4141 4141 Size: 6805 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2698 2698 Size: 6805 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6805 XmaI C'CCGG_G Cuts at: 1870 4130 1870 Size: 2260 4545 XmnI GAAnn'nnTTC Cuts at: 4934 4934 Size: 6805 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4613 276 Size: 53 83 186 936 3079 2468 Fragments arranged by size: 3079 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SfiI SrfI SwaI