(Circular) (Six-base) MAPSORT of: pFN21ASDA0948.seq Check: 8747 from: 1 to: 6292 pFN21ASDA0948 6292 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2224 2908 2224 Size: 684 5608 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3489 4102 278 Size: 53 83 186 936 1953 613 2468 Fragments arranged by size: 2468 1953 936 613 186 83 53 AccI GT'mk_AC Cuts at: 1937 3635 1937 Size: 1698 4594 Acc65I G'GTAC_C Cuts at: 2453 3613 2453 Size: 1160 5132 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2845 3212 4355 5739 6007 1632 Size: 1213 367 1143 1384 268 1917 Fragments arranged by size: 1917 1384 1213 1143 367 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 5191 6168 1203 Size: 3988 977 1327 Fragments arranged by size: 3988 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 2693 1166 Size: 1527 4765 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3440 1184 Size: 2256 4036 AlwNI CAG_nnn'CTG Cuts at: 2629 3052 4035 5607 2629 Size: 423 983 1572 3314 Fragments arranged by size: 3314 1572 983 423 ApaI G_GGCC'C Cuts at: 2938 3138 2938 Size: 200 6092 ApaLI G'TGCA_C Cuts at: 4349 5505 4349 Size: 1156 5136 ApoI r'AATT_y Cuts at: 1493 2993 3601 3827 3979 6088 1493 Size: 1500 608 226 152 2109 1697 Fragments arranged by size: 2109 1697 1500 608 226 152 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 3089 3097 3227 3617 1870 Size: 73 1146 8 130 390 4545 Fragments arranged by size: 4545 1146 390 130 73 8 BamHI G'GATC_C Cuts at: 3622 3989 3622 Size: 367 5925 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2453 3316 3533 3613 5068 Size: 325 217 627 666 863 217 80 1455 Cuts at: 5068 618 Size: 1842 Fragments arranged by size: 1842 1455 863 666 627 325 217 217 80 BanII G_rGCy'C Cuts at: 1391 2277 2938 3138 3611 1391 Size: 886 661 200 473 4072 Fragments arranged by size: 4072 886 661 473 200 BbeI G_GCGC'C Cuts at: 3537 3537 Size: 6292 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2757 928 Size: 971 858 4463 Fragments arranged by size: 4463 971 858 BbvCI CC'TCA_GC Cuts at: 2146 3139 3264 3570 2146 Size: 993 125 306 4868 Fragments arranged by size: 4868 993 306 125 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2034 2068 4483 4517 1903 Size: 34 97 34 2415 34 3678 Fragments arranged by size: 3678 2415 97 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 5394 4184 Size: 1210 5082 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2582 4903 136 Size: 107 122 71 631 605 910 2321 1525 Fragments arranged by size: 2321 1525 910 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 2637 3709 2637 Size: 1072 5220 Bme1580I G_kGCm'C Cuts at: 1790 2938 3138 4353 5509 1790 Size: 1148 200 1215 1156 2573 Fragments arranged by size: 2573 1215 1156 1148 200 BmgBI CAC'GTC Cuts at: 1318 2761 1318 Size: 1443 4849 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3348 4023 4981 Size: 416 53 83 399 24 1931 675 958 Cuts at: 4981 442 Size: 1753 Fragments arranged by size: 1931 1753 958 675 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3377 1056 Size: 2321 3971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2635 2635 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2079 2300 2924 3101 3342 4952 1239 Size: 840 221 624 177 241 1610 2579 Fragments arranged by size: 2579 1610 840 624 241 221 177 Bpu10I CC'TnA_GC Cuts at: 2146 3139 3264 3570 2146 Size: 993 125 306 4868 Fragments arranged by size: 4868 993 306 125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2543 3497 3527 4417 5282 5580 2543 Size: 954 30 890 865 298 3255 Fragments arranged by size: 3255 954 890 865 298 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4955 882 Size: 617 3456 2219 Fragments arranged by size: 3456 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2052 3589 4083 493 Size: 711 848 1537 494 2702 Fragments arranged by size: 2702 1537 848 711 494 BsaBI GATnn'nnATC Cuts at: 6286 6286 Size: 6292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3486 3534 4099 4481 Size: 53 83 186 936 1953 48 565 382 Cuts at: 4481 5116 275 Size: 635 1451 Fragments arranged by size: 1953 1451 936 635 565 382 186 83 53 48 BsaWI w'CCGG_w Cuts at: 4725 5123 5397 5544 5955 4725 Size: 398 274 147 411 5062 Fragments arranged by size: 5062 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2504 2534 1178 Size: 30 165 30 1101 30 4936 Fragments arranged by size: 4936 1101 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 3587 1186 Size: 1099 1302 3891 Fragments arranged by size: 3891 1302 1099 BseYI C'CCAG_C Cuts at: 2470 2881 3202 3249 3330 3427 5495 5991 2470 Size: 411 321 47 81 97 2068 496 2771 Fragments arranged by size: 2771 2068 496 411 321 97 81 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2821 1163 Size: 573 1085 4634 Fragments arranged by size: 4634 1085 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3742 4503 5531 1540 Size: 275 186 1741 761 1028 2301 Fragments arranged by size: 2301 1741 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 4353 4438 5509 2277 Size: 2076 85 1071 3060 Fragments arranged by size: 3060 2076 1071 85 BsmI GAATG_Cn' Cuts at: 3803 3896 3803 Size: 93 6199 BsmBI CGTCTCn'nnnn_ Cuts at: 3179 3179 Size: 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2938 3138 3611 4353 4438 5509 Size: 399 487 661 200 473 742 85 1071 Cuts at: 5509 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 661 487 473 399 200 85 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 2703 4181 5911 2703 Size: 1478 1730 3084 Fragments arranged by size: 3084 1730 1478 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2224 2908 3647 844 Size: 1380 684 739 3489 Fragments arranged by size: 3489 1380 739 684 BsrBI CCG'CTC Cuts at: 1394 1823 4179 1394 Size: 429 2356 3507 Fragments arranged by size: 3507 2356 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2331 4787 4961 65 Size: 1183 1083 2456 174 1396 Fragments arranged by size: 2456 1396 1183 1083 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2191 4936 5123 1700 Size: 110 381 2745 187 2869 Fragments arranged by size: 2869 2745 381 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 2629 794 Size: 1835 4457 BstBI TT'CG_AA Cuts at: 2007 5096 2007 Size: 3089 3203 BstEII G'GTnAC_C Cuts at: 1184 3157 1184 Size: 1973 4319 BstXI CCAn_nnnn'nTGG Cuts at: 3338 3338 Size: 6292 BstYI r'GATC_y Cuts at: 1972 3622 3989 4374 4391 5843 5929 5941 6287 Size: 1650 367 385 17 1452 86 12 346 Cuts at: 6287 1972 Size: 1977 Fragments arranged by size: 1977 1650 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2088 513 Size: 552 957 66 4717 Fragments arranged by size: 4717 957 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2059 487 Size: 683 56 833 4720 Fragments arranged by size: 4720 833 683 56 BtsI GCAGTG_nn' Cuts at: 3069 3808 4602 4622 3069 Size: 739 794 20 4739 Fragments arranged by size: 4739 794 739 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6257 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DraIII CAC_nnn'GTG Cuts at: 3460 3460 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2759 5299 809 Size: 507 1443 2540 1802 Fragments arranged by size: 2540 1802 1443 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2101 3234 3579 Size: 54 1425 50 275 18 271 1133 345 Cuts at: 3579 3739 4628 6257 8 Size: 160 889 1629 43 Fragments arranged by size: 1629 1425 1133 889 345 275 271 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4222 1353 Size: 2869 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 2275 Size: 6292 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2300 2845 2924 3101 3212 3342 Size: 393 447 221 545 79 177 111 130 Cuts at: 3342 4355 4952 5739 6007 1239 Size: 1013 597 787 268 1524 Fragments arranged by size: 1524 1013 787 597 545 447 393 268 221 177 130 111 79 EcoNI CCTnn'n_nnAGG Cuts at: 2606 3170 2606 Size: 564 5728 EcoO109I rG'GnC_Cy Cuts at: 2080 2935 3101 3135 2080 Size: 855 166 34 5237 Fragments arranged by size: 5237 855 166 34 EcoRI G'AATT_C Cuts at: 3601 3979 3601 Size: 378 5914 FspI TGC'GCA Cuts at: 3557 4798 3557 Size: 1241 5051 HaeII r_GCGC'y Cuts at: 1678 3213 3537 5439 1678 Size: 1535 324 1902 2531 Fragments arranged by size: 2531 1902 1535 324 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2504 2536 4933 4965 5007 Size: 32 135 32 932 32 2397 32 42 Cuts at: 5007 5039 1373 Size: 32 2626 Fragments arranged by size: 2626 2397 932 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3636 3882 669 Size: 988 281 1698 246 3079 Fragments arranged by size: 3079 1698 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6292 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KasI G'GCGC_C Cuts at: 3533 3533 Size: 6292 KpnI G_GTAC'C Cuts at: 2457 3617 2457 Size: 1160 5132 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2645 5406 5590 1305 Size: 1340 2761 184 2007 Fragments arranged by size: 2761 2007 1340 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3236 3581 10 Size: 54 1425 343 1404 345 2721 Fragments arranged by size: 2721 1425 1404 345 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2693 4250 4609 4768 518 Size: 648 1527 1557 359 159 2042 Fragments arranged by size: 2042 1557 1527 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2541 3708 4385 5533 5778 1606 Size: 935 1167 677 1148 245 2120 Fragments arranged by size: 2120 1167 1148 935 677 245 NarI GG'CG_CC Cuts at: 3534 3534 Size: 6292 NcoI C'CATG_G Cuts at: 513 1065 2022 2088 513 Size: 552 957 66 4717 Fragments arranged by size: 4717 957 552 66 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NheI G'CTAG_C Cuts at: 1052 3373 1052 Size: 2321 3971 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 2551 3650 5195 1132 Size: 97 1322 1099 1545 2229 Fragments arranged by size: 2229 1545 1322 1099 97 PasI CC'CwG_GG Cuts at: 1348 3131 1348 Size: 1783 4509 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6292 PfoI T'CCnGG_A Cuts at: 2277 2277 Size: 6292 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PmlI CAC'GTG Cuts at: 2052 2052 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2840 2872 4361 4393 5874 5906 2840 Size: 32 1489 32 1481 32 3226 Fragments arranged by size: 3226 1489 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3101 3101 Size: 6292 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PspOMI G'GGCC_C Cuts at: 2934 3134 2934 Size: 200 6092 PspXI vC'TCGA_Gb Cuts at: 3227 3227 Size: 6292 PstI C_TGCA'G Cuts at: 830 2520 2919 2952 3183 3469 3644 830 Size: 1690 399 33 231 286 175 3478 Fragments arranged by size: 3478 1690 399 286 231 175 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 SacI G_AGCT'C Cuts at: 2277 2277 Size: 6292 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SanDI GG'GwC_CC Cuts at: 3101 3101 Size: 6292 SbfI CC_TGCA'GG Cuts at: 2919 3644 2919 Size: 725 5567 ScaI AGT'ACT Cuts at: 3095 4540 6252 3095 Size: 1445 1712 3135 Fragments arranged by size: 3135 1712 1445 SexAI A'CCwGG_T Cuts at: 2034 2925 2034 Size: 891 5401 SfcI C'TryA_G Cuts at: 826 1045 2516 2915 2948 3179 3465 3640 4775 Size: 219 1471 399 33 231 286 175 1135 Cuts at: 4775 5456 5647 826 Size: 681 191 1471 Fragments arranged by size: 1471 1471 1135 681 399 286 231 219 191 175 33 SfoI GGC'GCC Cuts at: 3535 3535 Size: 6292 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2558 3227 3512 3542 4396 5297 Size: 197 926 615 669 285 30 854 901 Cuts at: 5297 5559 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 854 669 615 285 262 197 30 SnaBI TAC'GTA Cuts at: 493 3589 4083 493 Size: 3096 494 2702 Fragments arranged by size: 3096 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 2551 3650 1132 Size: 1419 1099 3774 Fragments arranged by size: 3774 1419 1099 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 2504 2901 1517 Size: 987 397 4908 Fragments arranged by size: 4908 987 397 StyI C'CwwG_G Cuts at: 513 1065 2022 2088 2499 2508 3731 513 Size: 552 957 66 411 9 1223 3074 Fragments arranged by size: 3074 1223 957 552 411 66 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2173 2431 3241 4319 4336 4489 4674 Size: 1255 593 258 810 1078 17 153 185 Cuts at: 4674 5119 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1078 810 593 445 258 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2562 3093 4538 Size: 274 80 33 51 1443 585 531 1445 Cuts at: 4538 6250 96 Size: 1712 138 Fragments arranged by size: 1712 1445 1443 585 531 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2915 2947 6252 6284 2915 Size: 32 3305 32 2923 Fragments arranged by size: 3305 2923 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6292 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3076 3498 3076 Size: 422 5870 XhoI C'TCGA_G Cuts at: 1943 3227 1943 Size: 1284 5008 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 3041 4421 3041 Size: 1380 4912 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3487 4100 276 Size: 53 83 186 936 1953 613 2468 Fragments arranged by size: 2468 1953 936 613 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI PvuII RsrII SacII SapI SfiI SrfI SwaI