(Circular) (Six-base) MAPSORT of: pFN21ASDA0940.seq Check: 293 from: 1 to: 6781 pFN21ASDA0940 6781 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4591 278 Size: 53 83 186 936 3055 2468 Fragments arranged by size: 3055 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3387 4124 1937 Size: 1450 737 4594 Fragments arranged by size: 4594 1450 737 Acc65I G'GTAC_C Cuts at: 4102 4102 Size: 6781 AclI AA'CG_TT Cuts at: 1563 4908 5281 1563 Size: 3345 373 3063 Fragments arranged by size: 3345 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3761 3809 4844 6228 6496 1632 Size: 2129 48 1035 1384 268 1917 Fragments arranged by size: 2129 1917 1384 1035 268 48 AfeI AGC'GCT Cuts at: 3808 3808 Size: 6781 AflII C'TTAA_G Cuts at: 820 1017 3250 820 Size: 197 2233 4351 Fragments arranged by size: 4351 2233 197 AflIII A'CryG_T Cuts at: 1203 5680 6657 1203 Size: 4477 977 1327 Fragments arranged by size: 4477 1327 977 AgeI A'CCGG_T Cuts at: 5612 5612 Size: 6781 AhdI GACnn_n'nnGTC Cuts at: 5510 5510 Size: 6781 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6781 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6781 AlwNI CAG_nnn'CTG Cuts at: 2201 2352 2708 4524 6096 2201 Size: 151 356 1816 1572 2886 Fragments arranged by size: 2886 1816 1572 356 151 ApaLI G'TGCA_C Cuts at: 4838 5994 4838 Size: 1156 5625 ApoI r'AATT_y Cuts at: 1493 3133 3558 4090 4316 4468 6577 1493 Size: 1640 425 532 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 532 425 226 152 AseI AT'TA_AT Cuts at: 160 1032 5335 160 Size: 872 4303 1606 Fragments arranged by size: 4303 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 AvaI C'yCGr_G Cuts at: 1870 1943 3996 4106 1870 Size: 73 2053 110 4545 Fragments arranged by size: 4545 2053 110 73 BamHI G'GATC_C Cuts at: 4111 4478 4111 Size: 367 6414 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2314 2363 2617 2866 4102 Size: 325 217 627 527 49 254 249 1236 Cuts at: 4102 5557 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1236 627 527 325 254 249 217 49 BanII G_rGCy'C Cuts at: 1391 2310 4100 1391 Size: 919 1790 4072 Fragments arranged by size: 4072 1790 919 BbeI G_GCGC'C Cuts at: 2621 2621 Size: 6781 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2452 2682 928 Size: 971 553 230 5027 Fragments arranged by size: 5027 971 553 230 BbvCI CC'TCA_GC Cuts at: 2189 2283 2189 Size: 94 6687 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2905 2939 4972 5006 1903 Size: 34 968 34 2033 34 3678 Fragments arranged by size: 3678 2033 968 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2128 4673 5883 2128 Size: 2545 1210 3026 Fragments arranged by size: 3026 2545 1210 BclI T'GATC_A Cuts at: 1548 3100 3229 3681 3877 1548 Size: 1552 129 452 196 4452 Fragments arranged by size: 4452 1552 452 196 129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2210 2645 2732 Size: 107 122 71 631 605 538 435 87 Cuts at: 2732 5392 136 Size: 2660 1525 Fragments arranged by size: 2660 1525 631 605 538 435 122 107 87 71 BglII A'GATC_T Cuts at: 6776 6776 Size: 6781 BlpI GC'TnA_GC Cuts at: 4198 4198 Size: 6781 Bme1580I G_kGCm'C Cuts at: 1790 2366 3905 4842 5998 1790 Size: 576 1539 937 1156 2573 Fragments arranged by size: 2573 1539 1156 937 576 BmgBI CAC'GTC Cuts at: 1318 4057 1318 Size: 2739 4042 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2402 2450 4512 Size: 416 53 83 399 24 985 48 2062 Cuts at: 4512 5470 442 Size: 958 1753 Fragments arranged by size: 2062 1753 985 958 416 399 83 53 48 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6781 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3481 3481 Size: 6781 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2797 2896 4090 5441 1239 Size: 1558 99 1194 1351 2579 Fragments arranged by size: 2579 1558 1351 1194 99 Bpu10I CC'TnA_GC Cuts at: 2189 2283 2189 Size: 94 6687 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2284 4906 5771 6069 2284 Size: 2622 865 298 2996 Fragments arranged by size: 2996 2622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2144 5444 882 Size: 617 645 3300 2219 Fragments arranged by size: 3300 2219 645 617 BsaAI yAC'GTr Cuts at: 493 1204 4078 4572 493 Size: 711 2874 494 2702 Fragments arranged by size: 2874 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6775 6775 Size: 6781 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2618 4588 4970 5605 Size: 53 83 186 936 1085 1970 382 635 Cuts at: 5605 275 Size: 1451 Fragments arranged by size: 1970 1451 1085 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2991 3030 5214 5612 5886 6033 6444 2991 Size: 39 2184 398 274 147 411 3328 Fragments arranged by size: 3328 2184 411 398 274 147 39 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6556 Fragments arranged by size: 6556 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2134 2271 2647 1186 Size: 948 137 376 5320 Fragments arranged by size: 5320 948 376 137 BseYI C'CCAG_C Cuts at: 2058 2064 2070 2194 2502 2609 3978 5984 6480 Size: 6 6 124 308 107 1369 2006 496 Cuts at: 6480 2058 Size: 2359 Fragments arranged by size: 2359 2006 1369 496 308 124 107 6 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3839 1163 Size: 573 2103 4105 Fragments arranged by size: 4105 2103 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2838 4045 4231 4992 6020 1540 Size: 275 186 837 1207 186 761 1028 2301 Fragments arranged by size: 2301 1207 1028 837 761 275 186 186 BsiHKAI G_wGCw'C Cuts at: 4842 4927 5998 4842 Size: 85 1071 5625 Fragments arranged by size: 5625 1071 85 BsiWI C'GTAC_G Cuts at: 2649 2649 Size: 6781 BsmI GAATG_Cn' Cuts at: 2773 3770 4292 4385 2773 Size: 997 522 93 5169 Fragments arranged by size: 5169 997 522 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2366 3905 4100 4842 4927 5998 Size: 399 520 56 1539 195 742 85 1071 Cuts at: 5998 1391 Size: 2174 Fragments arranged by size: 2174 1539 1071 742 520 399 195 85 56 BspEI T'CCGG_A Cuts at: 2991 6444 2991 Size: 3453 3328 BspHI T'CATG_A Cuts at: 2667 3103 4670 6400 2667 Size: 436 1567 1730 3048 Fragments arranged by size: 3048 1730 1567 436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3357 4136 844 Size: 2513 779 3489 Fragments arranged by size: 3489 2513 779 BsrBI CCG'CTC Cuts at: 1394 1823 2251 2595 2878 2896 4668 1394 Size: 429 428 344 283 18 1772 3507 Fragments arranged by size: 3507 1772 429 428 344 283 18 BsrDI GCAATG_nn' Cuts at: 65 1248 3684 3792 5276 5450 65 Size: 1183 2436 108 1484 174 1396 Fragments arranged by size: 2436 1484 1396 1183 174 108 BsrFI r'CCGG_y Cuts at: 1700 1810 2182 2545 5425 5612 1700 Size: 110 372 363 2880 187 2869 Fragments arranged by size: 2880 2869 372 363 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6781 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6781 BssSI C'ACGA_G Cuts at: 4841 5853 4841 Size: 1012 5769 BstAPI GCAn_nnn'nTGC Cuts at: 794 2708 794 Size: 1914 4867 BstBI TT'CG_AA Cuts at: 2007 5585 2007 Size: 3578 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6781 BstYI r'GATC_y Cuts at: 1972 4111 4478 4863 4880 6332 6418 6430 6776 Size: 2139 367 385 17 1452 86 12 346 Cuts at: 6776 1972 Size: 1977 Fragments arranged by size: 2139 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2301 2301 Size: 6781 BtgI C'CryG_G Cuts at: 513 1065 2310 2358 2643 3223 3566 513 Size: 552 1245 48 285 580 343 3728 Fragments arranged by size: 3728 1245 580 552 343 285 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6042 Fragments arranged by size: 6042 683 56 BtsI GCAGTG_nn' Cuts at: 2184 3351 4297 5091 5111 2184 Size: 1167 946 794 20 3854 Fragments arranged by size: 3854 1167 946 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6781 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6746 DraI TTT'AAA Cuts at: 4085 4085 Size: 6781 DraIII CAC_nnn'GTG Cuts at: 3911 3911 Size: 6781 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5788 809 Size: 507 4472 1802 Fragments arranged by size: 4472 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2653 3760 4228 Size: 54 1425 50 275 18 823 1107 468 Cuts at: 4228 5117 6746 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1107 889 823 468 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4228 1537 Size: 275 2416 4090 Fragments arranged by size: 4090 2416 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2432 4711 1353 Size: 1079 2279 3423 Fragments arranged by size: 3423 2279 1079 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5368 5742 5888 387 Size: 1064 138 3779 374 146 1280 Fragments arranged by size: 3779 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2797 2896 3761 3809 4090 4844 5441 Size: 393 1165 99 865 48 281 754 597 Cuts at: 5441 6228 6496 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1165 865 787 754 597 393 281 268 99 48 EcoNI CCTnn'n_nnAGG Cuts at: 3114 3429 3114 Size: 315 6466 EcoO109I rG'GnC_Cy Cuts at: 2821 3039 2821 Size: 218 6563 EcoRI G'AATT_C Cuts at: 4090 4468 4090 Size: 378 6403 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3432 3432 Size: 6781 FspI TGC'GCA Cuts at: 5287 5287 Size: 6781 HaeII r_GCGC'y Cuts at: 1678 2593 2621 2640 3810 5928 1678 Size: 915 28 19 1170 2118 2531 Fragments arranged by size: 2531 2118 1170 915 28 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2257 2289 3171 3203 5422 Size: 32 135 32 685 32 882 32 2219 Cuts at: 5422 5454 5496 5528 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2219 882 685 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2413 2452 4125 4371 669 Size: 988 281 475 39 1673 246 3079 Fragments arranged by size: 3079 1673 988 475 281 246 39 HindIII A'AGCT_T Cuts at: 748 3474 748 Size: 2726 4055 HpaI GTT'AAC Cuts at: 4371 4371 Size: 6781 KasI G'GCGC_C Cuts at: 2617 2617 Size: 6781 KpnI G_GTAC'C Cuts at: 4106 4106 Size: 6781 MfeI C'AATT_G Cuts at: 4380 4380 Size: 6781 MluI A'CGCG_T Cuts at: 6657 6657 Size: 6781 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2814 3048 3295 5895 6079 1305 Size: 1509 234 247 2600 184 2007 Fragments arranged by size: 2600 2007 1509 247 234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4959 Fragments arranged by size: 4959 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4739 5098 5257 518 Size: 648 3573 359 159 2042 Fragments arranged by size: 3573 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2083 2201 2312 2645 2708 2745 4024 4068 Size: 477 118 111 333 63 37 1279 44 Cuts at: 4068 4197 4874 6022 6267 1606 Size: 129 677 1148 245 2120 Fragments arranged by size: 2120 1279 1148 677 477 333 245 129 118 111 63 44 37 NaeI GCC'GGC Cuts at: 2184 2547 2184 Size: 363 6418 NarI GG'CG_CC Cuts at: 2618 2618 Size: 6781 NcoI C'CATG_G Cuts at: 513 1065 3223 3566 513 Size: 552 2158 343 3728 Fragments arranged by size: 3728 2158 552 343 NdeI CA'TA_TG Cuts at: 387 3865 387 Size: 3478 3303 NgoMIV G'CCGG_C Cuts at: 2182 2545 2182 Size: 363 6418 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6781 NotI GC'GGCC_GC Cuts at: 4228 4228 Size: 6781 NspI r_CATG'y Cuts at: 1132 1229 4139 5684 1132 Size: 97 2910 1545 2229 Fragments arranged by size: 2910 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2796 1348 Size: 1448 5333 PciI A'CATG_T Cuts at: 5680 5680 Size: 6781 PflMI CCAn_nnn'nTGG Cuts at: 1108 2796 2970 1108 Size: 1688 174 4919 Fragments arranged by size: 4919 1688 174 PfoI T'CCnGG_A Cuts at: 2969 3145 3210 2969 Size: 176 65 6540 Fragments arranged by size: 6540 176 65 PmeI GTTT'AAAC Cuts at: 4085 4085 Size: 6781 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4850 4882 6363 6395 4850 Size: 32 1481 32 5236 Fragments arranged by size: 5236 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2821 2821 Size: 6781 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6781 PsiI TTA'TAA Cuts at: 4351 4351 Size: 6781 PstI C_TGCA'G Cuts at: 830 2811 3960 4133 830 Size: 1981 1149 173 3478 Fragments arranged by size: 3478 1981 1149 173 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6781 PvuII CAG'CTG Cuts at: 2745 2745 Size: 6781 RsrII CG'GwC_CG Cuts at: 2994 2994 Size: 6781 SacII CC_GC'GG Cuts at: 2313 2646 2313 Size: 333 6448 SalI G'TCGA_C Cuts at: 1936 4123 1936 Size: 2187 4594 SanDI GG'GwC_CC Cuts at: 2821 2821 Size: 6781 SapI GCTCTTCn'nnn_ Cuts at: 2432 2432 Size: 6781 SbfI CC_TGCA'GG Cuts at: 2811 4133 2811 Size: 1322 5459 ScaI AGT'ACT Cuts at: 5029 6741 5029 Size: 1712 5069 SexAI A'CCwGG_T Cuts at: 2351 2351 Size: 6781 SfcI C'TryA_G Cuts at: 826 1045 2807 3956 4129 5264 5945 6136 826 Size: 219 1762 1149 173 1135 681 191 1471 Fragments arranged by size: 1762 1471 1149 1135 681 219 191 173 SfoI GGC'GCC Cuts at: 2619 2619 Size: 6781 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6781 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6781 SmaI CCC'GGG Cuts at: 1872 4108 1872 Size: 2236 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2263 3250 4885 5786 6048 820 Size: 197 926 320 987 1635 901 262 1553 Fragments arranged by size: 1635 1553 987 926 901 320 262 197 SnaBI TAC'GTA Cuts at: 493 4078 4572 493 Size: 3585 494 2702 Fragments arranged by size: 3585 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6781 SphI G_CATG'C Cuts at: 1132 4139 1132 Size: 3007 3774 SspI AAT'ATT Cuts at: 5 52 3160 4705 5 Size: 47 3108 1545 2081 Fragments arranged by size: 3108 2081 1545 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6781 StyI C'CwwG_G Cuts at: 513 1065 3223 3566 4220 513 Size: 552 2158 343 654 3074 Fragments arranged by size: 3074 2158 654 552 343 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2840 2846 3011 4808 4825 4978 5163 Size: 1255 1260 6 165 1797 17 153 185 Cuts at: 5163 5608 325 Size: 445 1498 Fragments arranged by size: 1797 1498 1260 1255 445 185 165 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3469 5027 6739 Size: 274 80 33 51 1443 1492 1558 1712 Cuts at: 6739 96 Size: 138 Fragments arranged by size: 1712 1558 1492 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3562 3594 6741 6773 3562 Size: 32 3147 32 3570 Fragments arranged by size: 3570 3147 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6781 XbaI T'CTAG_A Cuts at: 3299 4117 3299 Size: 818 5963 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2971 2971 Size: 6781 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6781 XmaI C'CCGG_G Cuts at: 1870 4106 1870 Size: 2236 4545 XmnI GAAnn'nnTTC Cuts at: 4910 4910 Size: 6781 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4589 276 Size: 53 83 186 936 3055 2468 Fragments arranged by size: 3055 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI SacI SfiI SrfI SwaI