(Circular) (Six-base) MAPSORT of: pFN21ASDA0936.seq Check: 6346 from: 1 to: 6625 pFN21ASDA0936 6625 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2995 2995 Size: 6625 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2571 3437 4435 278 Size: 53 83 186 936 1035 866 998 2468 Fragments arranged by size: 2468 1035 998 936 866 186 83 53 AccI GT'mk_AC Cuts at: 1937 2543 3968 1937 Size: 606 1425 4594 Fragments arranged by size: 4594 1425 606 Acc65I G'GTAC_C Cuts at: 3946 3946 Size: 6625 AclI AA'CG_TT Cuts at: 1563 4752 5125 1563 Size: 3189 373 3063 Fragments arranged by size: 3189 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2718 2801 2897 3407 3591 3800 4688 6072 Size: 1086 83 96 510 184 209 888 1384 Cuts at: 6072 6340 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1086 888 510 268 209 184 96 83 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6428 AflIII A'CryG_T Cuts at: 1203 5524 6501 1203 Size: 4321 977 1327 Fragments arranged by size: 4321 1327 977 AgeI A'CCGG_T Cuts at: 5456 5456 Size: 6625 AhdI GACnn_n'nnGTC Cuts at: 5354 5354 Size: 6625 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6625 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6625 AlwNI CAG_nnn'CTG Cuts at: 2803 4368 5940 2803 Size: 1565 1572 3488 Fragments arranged by size: 3488 1572 1565 ApaLI G'TGCA_C Cuts at: 4682 5838 4682 Size: 1156 5469 ApoI r'AATT_y Cuts at: 1493 2135 2655 3018 3557 3637 3934 4160 4312 Size: 642 520 363 539 80 297 226 152 Cuts at: 4312 6421 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 642 539 520 363 297 226 152 80 AseI AT'TA_AT Cuts at: 160 1032 5179 160 Size: 872 4147 1606 Fragments arranged by size: 4147 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 2051 2463 3950 1870 Size: 73 108 412 1487 4545 Fragments arranged by size: 4545 1487 412 108 73 BamHI G'GATC_C Cuts at: 2824 3955 4322 2824 Size: 1131 367 5127 Fragments arranged by size: 5127 1131 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3946 5401 618 Size: 325 217 627 2159 1455 1842 Fragments arranged by size: 2159 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2524 3944 1391 Size: 1133 1420 4072 Fragments arranged by size: 4072 1420 1133 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5654 BbvCI CC'TCA_GC Cuts at: 3311 3311 Size: 6625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4816 4850 1903 Size: 34 2879 34 3678 Fragments arranged by size: 3678 2879 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4517 5727 4517 Size: 1210 5415 BclI T'GATC_A Cuts at: 1548 2239 1548 Size: 691 5934 BfrBI ATG'CAT Cuts at: 2157 2157 Size: 6625 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5236 136 Size: 107 122 71 631 605 3564 1525 Fragments arranged by size: 3564 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3467 6620 3467 Size: 3153 3472 BlpI GC'TnA_GC Cuts at: 4042 4042 Size: 6625 Bme1580I G_kGCm'C Cuts at: 1790 3410 3424 4686 5842 1790 Size: 1620 14 1262 1156 2573 Fragments arranged by size: 2573 1620 1262 1156 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6625 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3901 4356 5314 Size: 416 53 83 399 24 2484 455 958 Cuts at: 5314 442 Size: 1753 Fragments arranged by size: 2484 1753 958 455 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2784 1056 Size: 1728 4897 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2508 2646 3073 3098 3212 3843 5285 1239 Size: 1269 138 427 25 114 631 1442 2579 Fragments arranged by size: 2579 1442 1269 631 427 138 114 25 Bpu10I CC'TnA_GC Cuts at: 3311 3311 Size: 6625 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4750 5615 5913 4750 Size: 865 298 5462 Fragments arranged by size: 5462 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3091 3784 5288 882 Size: 617 1592 693 1504 2219 Fragments arranged by size: 2219 1592 1504 693 617 BsaAI yAC'GTr Cuts at: 493 1204 3066 3922 4416 493 Size: 711 1862 856 494 2702 Fragments arranged by size: 2702 1862 856 711 494 BsaBI GATnn'nnATC Cuts at: 6619 6619 Size: 6625 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2568 3434 4432 4814 Size: 53 83 186 936 1035 866 998 382 Cuts at: 4814 5449 275 Size: 635 1451 Fragments arranged by size: 1451 1035 998 936 866 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5058 5456 5730 5877 6288 5058 Size: 398 274 147 411 5395 Fragments arranged by size: 5395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2162 2192 2540 2570 1178 Size: 30 165 30 759 30 348 30 5233 Fragments arranged by size: 5233 759 348 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 1186 Size: 1219 5406 BseYI C'CCAG_C Cuts at: 2080 2668 2991 3822 3867 5828 6324 2080 Size: 588 323 831 45 1961 496 2381 Fragments arranged by size: 2381 1961 831 588 496 323 45 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6052 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4075 4836 5864 1540 Size: 275 186 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3198 4686 4771 5842 3198 Size: 1488 85 1071 3981 Fragments arranged by size: 3981 1488 1071 85 BsmI GAATG_Cn' Cuts at: 2159 4136 4229 2159 Size: 1977 93 4555 Fragments arranged by size: 4555 1977 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2524 3198 3410 3424 3944 4686 4771 Size: 399 734 674 212 14 520 742 85 Cuts at: 4771 5842 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 734 674 520 399 212 85 14 BspEI T'CCGG_A Cuts at: 6288 6288 Size: 6625 BspHI T'CATG_A Cuts at: 4514 6244 4514 Size: 1730 4895 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2995 3354 3980 844 Size: 2151 359 626 3489 Fragments arranged by size: 3489 2151 626 359 BsrBI CCG'CTC Cuts at: 1394 1823 4512 1394 Size: 429 2689 3507 Fragments arranged by size: 3507 2689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2727 5120 5294 65 Size: 1183 1479 2393 174 1396 Fragments arranged by size: 2393 1479 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5269 5456 1700 Size: 110 3459 187 2869 Fragments arranged by size: 3459 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 4685 5697 4685 Size: 1012 5613 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6625 BstBI TT'CG_AA Cuts at: 2007 5429 2007 Size: 3422 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6625 BstYI r'GATC_y Cuts at: 1972 2824 3467 3955 4322 4707 4724 6176 6262 Size: 852 643 488 367 385 17 1452 86 Cuts at: 6262 6274 6620 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 852 643 488 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2537 2609 2537 Size: 72 6553 BtgI C'CryG_G Cuts at: 513 1065 3575 3851 513 Size: 552 2510 276 3287 Fragments arranged by size: 3287 2510 552 276 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 2791 4141 4935 4955 2791 Size: 1350 794 20 4461 Fragments arranged by size: 4461 1350 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6590 DraI TTT'AAA Cuts at: 2184 3929 2184 Size: 1745 4880 DraIII CAC_nnn'GTG Cuts at: 2744 2744 Size: 6625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5632 809 Size: 507 4316 1802 Fragments arranged by size: 4316 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2735 3880 4072 Size: 54 1425 50 275 18 905 1145 192 Cuts at: 4072 4961 6590 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1145 905 889 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4072 1537 Size: 275 2260 4090 Fragments arranged by size: 4090 2260 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2624 4555 1353 Size: 1271 1931 3423 Fragments arranged by size: 3423 1931 1271 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5212 5586 5732 387 Size: 1064 138 3623 374 146 1280 Fragments arranged by size: 3623 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 2646 2718 2801 2897 3073 3098 Size: 393 876 138 72 83 96 176 25 Cuts at: 3098 3212 3407 3591 3800 3843 4688 5285 6072 Size: 114 195 184 209 43 845 597 787 Cuts at: 6072 6340 1239 Size: 268 1524 Fragments arranged by size: 1524 876 845 787 597 393 268 209 195 184 176 138 114 96 83 72 43 25 EcoNI CCTnn'n_nnAGG Cuts at: 2168 2948 2168 Size: 780 5845 EcoRI G'AATT_C Cuts at: 3934 4312 3934 Size: 378 6247 EcoRV GAT'ATC Cuts at: 2864 2864 Size: 6625 FspI TGC'GCA Cuts at: 5131 5131 Size: 6625 HaeII r_GCGC'y Cuts at: 1678 5772 1678 Size: 4094 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2194 2486 2518 3424 Size: 32 135 32 590 32 292 32 906 Cuts at: 3424 3456 3781 3813 5266 5298 5340 5372 1373 Size: 32 325 32 1453 32 42 32 2626 Fragments arranged by size: 2626 1453 906 590 325 292 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3969 4215 669 Size: 988 281 1317 714 246 3079 Fragments arranged by size: 3079 1317 988 714 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6625 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KpnI G_GTAC'C Cuts at: 3950 3950 Size: 6625 MfeI C'AATT_G Cuts at: 4224 4224 Size: 6625 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2860 5739 5923 1305 Size: 1555 2879 184 2007 Fragments arranged by size: 2879 2007 1555 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2737 10 Size: 54 1425 343 905 3898 Fragments arranged by size: 3898 1425 905 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2202 3201 4583 4942 5101 518 Size: 648 1036 999 1382 359 159 2042 Fragments arranged by size: 2042 1382 1036 999 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3185 3445 4041 4718 5866 6111 1606 Size: 1579 260 596 677 1148 245 2120 Fragments arranged by size: 2120 1579 1148 677 596 260 245 NcoI C'CATG_G Cuts at: 513 1065 3575 513 Size: 552 2510 3563 Fragments arranged by size: 3563 2510 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6625 NheI G'CTAG_C Cuts at: 1052 2780 1052 Size: 1728 4897 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NsiI A_TGCA'T Cuts at: 2159 2159 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 2815 3983 5528 1132 Size: 97 1586 1168 1545 2229 Fragments arranged by size: 2229 1586 1545 1168 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6625 PciI A'CATG_T Cuts at: 5524 5524 Size: 6625 PflMI CCAn_nnn'nTGG Cuts at: 1108 2668 3878 1108 Size: 1560 1210 3855 Fragments arranged by size: 3855 1560 1210 PfoI T'CCnGG_A Cuts at: 2943 3089 3114 2943 Size: 146 25 6454 Fragments arranged by size: 6454 146 25 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4694 4726 6207 6239 4694 Size: 32 1481 32 5080 Fragments arranged by size: 5080 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 4195 4195 Size: 6625 PstI C_TGCA'G Cuts at: 830 3365 3475 3977 830 Size: 2535 110 502 3478 Fragments arranged by size: 3478 2535 502 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 PvuII CAG'CTG Cuts at: 3185 3185 Size: 6625 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SbfI CC_TGCA'GG Cuts at: 3977 3977 Size: 6625 ScaI AGT'ACT Cuts at: 2532 4873 6585 2532 Size: 2341 1712 2572 Fragments arranged by size: 2572 2341 1712 SfcI C'TryA_G Cuts at: 826 1045 2551 3361 3471 3973 5108 5789 5980 Size: 219 1506 810 110 502 1135 681 191 Cuts at: 5980 826 Size: 1471 Fragments arranged by size: 1506 1471 1135 810 681 502 219 191 110 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 3952 1872 Size: 2080 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4729 5630 5892 820 Size: 197 926 2786 901 262 1553 Fragments arranged by size: 2786 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3922 4416 493 Size: 3429 494 2702 Fragments arranged by size: 3429 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 3983 1132 Size: 2851 3774 SspI AAT'ATT Cuts at: 5 52 2649 4549 5 Size: 47 2597 1900 2081 Fragments arranged by size: 2597 2081 1900 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6625 StyI C'CwwG_G Cuts at: 513 1065 3575 4064 513 Size: 552 2510 489 3074 Fragments arranged by size: 3074 2510 552 489 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2901 3117 3443 3836 4652 4669 4822 Size: 1255 1321 216 326 393 816 17 153 Cuts at: 4822 5007 5452 325 Size: 185 445 1498 Fragments arranged by size: 1498 1321 1255 816 445 393 326 216 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2262 2530 4871 Size: 274 80 33 51 1443 285 268 2341 Cuts at: 4871 6583 96 Size: 1712 138 Fragments arranged by size: 2341 1712 1443 285 274 268 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6585 6617 6585 Size: 32 6593 Tth111I GACn'n_nGTC Cuts at: 1316 3616 1316 Size: 2300 4325 XbaI T'CTAG_A Cuts at: 3961 3961 Size: 6625 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3573 3875 3573 Size: 302 6323 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6625 XmaI C'CCGG_G Cuts at: 1870 3950 1870 Size: 2080 4545 XmnI GAAnn'nnTTC Cuts at: 3022 3561 3604 4754 3022 Size: 539 43 1150 4893 Fragments arranged by size: 4893 1150 539 43 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2569 3435 4433 276 Size: 53 83 186 936 1035 866 998 2468 Fragments arranged by size: 2468 1035 998 936 866 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI