(Circular) (Six-base) MAPSORT of: pFN21ASDA0923.seq Check: 9093 from: 1 to: 6571 pFN21ASDA0923 6571 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4381 278 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2656 3914 1937 Size: 719 1258 4594 Fragments arranged by size: 4594 1258 719 Acc65I G'GTAC_C Cuts at: 3892 3892 Size: 6571 AclI AA'CG_TT Cuts at: 1563 4698 5071 1563 Size: 3135 373 3063 Fragments arranged by size: 3135 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2046 2333 2471 2666 2784 3636 3741 4634 Size: 414 287 138 195 118 852 105 893 Cuts at: 4634 6018 6286 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 893 852 414 287 268 195 138 118 105 AflII C'TTAA_G Cuts at: 820 1017 2444 2852 820 Size: 197 1427 408 4539 Fragments arranged by size: 4539 1427 408 197 AflIII A'CryG_T Cuts at: 1203 3668 5470 6447 1203 Size: 2465 1802 977 1327 Fragments arranged by size: 2465 1802 1327 977 AgeI A'CCGG_T Cuts at: 5402 5402 Size: 6571 AhdI GACnn_n'nnGTC Cuts at: 5300 5300 Size: 6571 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6571 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6571 AlwNI CAG_nnn'CTG Cuts at: 2948 4314 5886 2948 Size: 1366 1572 3633 Fragments arranged by size: 3633 1572 1366 ApaLI G'TGCA_C Cuts at: 2168 3551 4628 5784 2168 Size: 1383 1077 1156 2955 Fragments arranged by size: 2955 1383 1156 1077 ApoI r'AATT_y Cuts at: 1493 2392 2524 2793 3063 3189 3355 3835 3880 Size: 899 132 269 270 126 166 480 45 Cuts at: 3880 4106 4258 6367 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 899 480 270 269 226 166 152 132 126 45 AseI AT'TA_AT Cuts at: 160 1032 5125 160 Size: 872 4093 1606 Fragments arranged by size: 4093 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 AvaI C'yCGr_G Cuts at: 1870 1943 3179 3556 3896 1870 Size: 73 1236 377 340 4545 Fragments arranged by size: 4545 1236 377 340 73 BamHI G'GATC_C Cuts at: 3901 4268 3901 Size: 367 6204 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3892 5347 618 Size: 325 217 627 2105 1455 1842 Fragments arranged by size: 2105 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3890 1391 Size: 2499 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2052 928 Size: 971 153 5447 Fragments arranged by size: 5447 971 153 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2149 2183 4762 4796 1903 Size: 34 212 34 2579 34 3678 Fragments arranged by size: 3678 2579 212 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4463 5673 4463 Size: 1210 5361 BclI T'GATC_A Cuts at: 1548 2230 1548 Size: 682 5889 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5182 136 Size: 107 122 71 631 605 3510 1525 Fragments arranged by size: 3510 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6566 6566 Size: 6571 BlpI GC'TnA_GC Cuts at: 3988 3988 Size: 6571 Bme1580I G_kGCm'C Cuts at: 1790 2172 3555 4632 5788 1790 Size: 382 1383 1077 1156 2573 Fragments arranged by size: 2573 1383 1156 1077 382 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6571 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4302 5260 442 Size: 416 53 83 399 24 2885 958 1753 Fragments arranged by size: 2885 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2449 2767 3585 5231 1239 Size: 1210 318 818 1646 2579 Fragments arranged by size: 2579 1646 1210 818 318 Bpu10I CC'TnA_GC Cuts at: 2370 2370 Size: 6571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3844 4696 5561 5859 3844 Size: 852 865 298 4556 Fragments arranged by size: 4556 865 852 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3603 5234 882 Size: 617 2104 1631 2219 Fragments arranged by size: 2219 2104 1631 617 BsaAI yAC'GTr Cuts at: 493 1204 3868 4362 493 Size: 711 2664 494 2702 Fragments arranged by size: 2702 2664 711 494 BsaBI GATnn'nnATC Cuts at: 6565 6565 Size: 6571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4378 4760 5395 275 Size: 53 83 186 936 2845 382 635 1451 Fragments arranged by size: 2845 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5004 5402 5676 5823 6234 5004 Size: 398 274 147 411 5341 Fragments arranged by size: 5341 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2740 2770 1178 Size: 30 165 30 1337 30 4979 Fragments arranged by size: 4979 1337 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3762 1186 Size: 2576 3995 BseYI C'CCAG_C Cuts at: 2401 5774 6270 2401 Size: 3373 496 2702 Fragments arranged by size: 3373 2702 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2176 1163 Size: 573 440 5558 Fragments arranged by size: 5558 573 440 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4021 4782 5810 1540 Size: 275 186 2020 761 1028 2301 Fragments arranged by size: 2301 2020 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 3555 4632 4717 5788 2172 Size: 1383 1077 85 1071 2955 Fragments arranged by size: 2955 1383 1077 1071 85 BsmI GAATG_Cn' Cuts at: 4082 4175 4082 Size: 93 6478 BsmBI CGTCTCn'nnnn_ Cuts at: 3320 3364 3320 Size: 44 6527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 3555 3890 4632 4717 5788 1391 Size: 399 382 1383 335 742 85 1071 2174 Fragments arranged by size: 2174 1383 1071 742 399 382 335 85 BspEI T'CCGG_A Cuts at: 6234 6234 Size: 6571 BspHI T'CATG_A Cuts at: 2608 4460 6190 2608 Size: 1852 1730 2989 Fragments arranged by size: 2989 1852 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3926 844 Size: 3082 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4458 1394 Size: 429 2635 3507 Fragments arranged by size: 3507 2635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2580 5066 5240 65 Size: 1183 1332 2486 174 1396 Fragments arranged by size: 2486 1396 1332 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5215 5402 1700 Size: 110 3405 187 2869 Fragments arranged by size: 3405 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3755 96 Size: 3659 2912 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6571 BssSI C'ACGA_G Cuts at: 4631 5643 4631 Size: 1012 5559 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6571 BstBI TT'CG_AA Cuts at: 2007 5375 2007 Size: 3368 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6571 BstYI r'GATC_y Cuts at: 1972 2047 3901 4268 4653 4670 6122 6208 6220 Size: 75 1854 367 385 17 1452 86 12 Cuts at: 6220 6566 1972 Size: 346 1977 Fragments arranged by size: 1977 1854 1452 385 367 346 86 75 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5062 Fragments arranged by size: 5062 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5832 Fragments arranged by size: 5832 683 56 BtsI GCAGTG_nn' Cuts at: 3203 4087 4881 4901 3203 Size: 884 794 20 4873 Fragments arranged by size: 4873 884 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6571 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6536 DraI TTT'AAA Cuts at: 3340 3875 3340 Size: 535 6036 DraIII CAC_nnn'GTG Cuts at: 3586 3586 Size: 6571 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5578 809 Size: 507 4262 1802 Fragments arranged by size: 4262 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4018 4907 6536 Size: 54 1425 50 275 18 2188 889 1629 Cuts at: 6536 8 Size: 43 Fragments arranged by size: 2188 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4018 1537 Size: 275 2206 4090 Fragments arranged by size: 4090 2206 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2612 2898 3636 4501 1353 Size: 1259 286 738 865 3423 Fragments arranged by size: 3423 1259 865 738 286 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5158 5532 5678 387 Size: 1064 138 3569 374 146 1280 Fragments arranged by size: 3569 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2046 2333 2449 2471 2666 2767 2784 Size: 393 414 287 116 22 195 101 17 Cuts at: 2784 3585 3636 3741 4634 5231 6018 6286 1239 Size: 801 51 105 893 597 787 268 1524 Fragments arranged by size: 1524 893 801 787 597 414 393 287 268 195 116 105 101 51 22 17 EcoNI CCTnn'n_nnAGG Cuts at: 2467 2467 Size: 6571 EcoO109I rG'GnC_Cy Cuts at: 3004 3004 Size: 6571 EcoRI G'AATT_C Cuts at: 3880 4258 3880 Size: 378 6193 FspI TGC'GCA Cuts at: 5077 5077 Size: 6571 HaeII r_GCGC'y Cuts at: 1678 5718 1678 Size: 4040 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3128 3160 3202 3234 5212 Size: 32 135 32 1556 32 42 32 1978 Cuts at: 5212 5244 5286 5318 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1978 1556 135 42 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3915 4161 669 Size: 988 281 1977 246 3079 Fragments arranged by size: 3079 1977 988 281 246 HindIII A'AGCT_T Cuts at: 748 3086 3376 748 Size: 2338 290 3943 Fragments arranged by size: 3943 2338 290 HpaI GTT'AAC Cuts at: 4161 4161 Size: 6571 KpnI G_GTAC'C Cuts at: 3896 3896 Size: 6571 MfeI C'AATT_G Cuts at: 4170 4170 Size: 6571 MluI A'CGCG_T Cuts at: 6447 6447 Size: 6571 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3279 3724 5685 5869 1305 Size: 1974 445 1961 184 2007 Fragments arranged by size: 2007 1974 1961 445 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4749 Fragments arranged by size: 4749 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4529 4888 5047 518 Size: 648 3363 359 159 2042 Fragments arranged by size: 3363 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2353 3987 4664 5812 6057 1606 Size: 747 1634 677 1148 245 2120 Fragments arranged by size: 2120 1634 1148 747 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5062 Fragments arranged by size: 5062 957 552 NdeI CA'TA_TG Cuts at: 387 3256 387 Size: 2869 3702 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6571 NotI GC'GGCC_GC Cuts at: 4018 4018 Size: 6571 NspI r_CATG'y Cuts at: 1132 1229 3613 3929 5474 1132 Size: 97 2384 316 1545 2229 Fragments arranged by size: 2384 2229 1545 316 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6571 PciI A'CATG_T Cuts at: 5470 5470 Size: 6571 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6571 PfoI T'CCnGG_A Cuts at: 2465 2744 2465 Size: 279 6292 PmeI GTTT'AAAC Cuts at: 3875 3875 Size: 6571 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4640 4672 6153 6185 4640 Size: 32 1481 32 5026 Fragments arranged by size: 5026 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3004 3004 Size: 6571 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6571 PsiI TTA'TAA Cuts at: 4141 4141 Size: 6571 PspXI vC'TCGA_Gb Cuts at: 3179 3556 3179 Size: 377 6194 PstI C_TGCA'G Cuts at: 830 3923 830 Size: 3093 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6571 SalI G'TCGA_C Cuts at: 1936 3913 1936 Size: 1977 4594 SbfI CC_TGCA'GG Cuts at: 3923 3923 Size: 6571 ScaI AGT'ACT Cuts at: 4819 6531 4819 Size: 1712 4859 SfcI C'TryA_G Cuts at: 826 1045 2423 3808 3919 5054 5735 5926 826 Size: 219 1378 1385 111 1135 681 191 1471 Fragments arranged by size: 1471 1385 1378 1135 681 219 191 111 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6571 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6571 SmaI CCC'GGG Cuts at: 1872 3898 1872 Size: 2026 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 2852 3179 3556 3823 4675 Size: 197 926 501 408 327 377 267 852 Cuts at: 4675 5576 5838 820 Size: 901 262 1553 Fragments arranged by size: 1553 926 901 852 501 408 377 327 267 262 197 SnaBI TAC'GTA Cuts at: 493 3868 4362 493 Size: 3375 494 2702 Fragments arranged by size: 3375 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6571 SphI G_CATG'C Cuts at: 1132 3929 1132 Size: 2797 3774 SspI AAT'ATT Cuts at: 5 52 2206 4495 5 Size: 47 2154 2289 2081 Fragments arranged by size: 2289 2154 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6571 StyI C'CwwG_G Cuts at: 513 1065 2022 4010 513 Size: 552 957 1988 3074 Fragments arranged by size: 3074 1988 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4598 4615 4768 4953 5398 325 Size: 1255 3018 17 153 185 445 1498 Fragments arranged by size: 3018 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2317 2939 3703 Size: 274 80 33 51 1443 340 622 764 Cuts at: 3703 3755 4817 6529 96 Size: 52 1062 1712 138 Fragments arranged by size: 1712 1443 1062 764 622 340 274 138 80 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6531 6563 6531 Size: 32 6539 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6571 XbaI T'CTAG_A Cuts at: 3907 3907 Size: 6571 XhoI C'TCGA_G Cuts at: 1943 3179 3556 1943 Size: 1236 377 4958 Fragments arranged by size: 4958 1236 377 XmaI C'CCGG_G Cuts at: 1870 3896 1870 Size: 2026 4545 XmnI GAAnn'nnTTC Cuts at: 3506 4700 3506 Size: 1194 5377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4379 276 Size: 53 83 186 936 2845 2468 Fragments arranged by size: 2845 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI